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Citations for Mirus Bio :
51 - 100 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and TransIT-LT1 (Mirus MIR 2300) transfection reagent ...
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bioRxiv - Immunology 2024Quote: ... LT1 transfection reagent (Mirus Bio, Madison, WI), and the pCL-ECO packaging plasmid ...
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bioRxiv - Genetics 2024Quote: ... using TransIT-LT1 (Mirus) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using TransIT-LT1 (Mirus). Supernatants were collected at 24 and 48 h ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Microbiology 2024Quote: ... and L segments using the TransIT-LT1transfection reagent (Mirus Bio) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and a CMV-Renilla plasmid using Mirus Transit X2 (Mirus MIR6004). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... cells in one 35 mm dish were either transiently transfected with the construct to be imaged using TransIT-293 lipofection reagent (Mirus Bio) or were virally transduced with a lentivirus ...
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bioRxiv - Microbiology 2024Quote: ... TransIT LTI (Mirus Bio) was used to transfect 30ng of either IFNβ promoter or ISRE promoter-firefly luciferase reporters and 30ng pRL-TK Renilla luciferase ...
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bioRxiv - Microbiology 2024Quote: ... A ratio of 1ug DNA to 3ul TransIT LTI (Mirus Bio, Madison, WI, USA) was used to transfect cells with 20.8ng VP35 ...
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bioRxiv - Microbiology 2024Quote: ... pTM-P and 1 pTM-L using TransIT-LT1 (Mirus) and incubated at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.2µl TransIT-X2 Dynamic Delivery System (Mirus, 6003) and supplement-free DMEM and incubated at RT for 20mins ...
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bioRxiv - Immunology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
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bioRxiv - Cell Biology 2024Quote: Transfection of Capsaspora cells and stable cell line generation was done using the TransIT-X2 transfection reagent (Mirus Bio) as previously described (Phillips et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... into the HEK293T cells by using the TransIT-Lenti transfection reagent (Mirus Bio). Lentiviral transduction of the CRISPR construct was performed with 10 µg/mL polybrene (ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 μl of Ingenio electroporation solution (Mirus, MIR50114) and nucleofected with an Amaxa Nucleofector I (Lonza ...
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bioRxiv - Immunology 2024Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7Lμg of the vector ...
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bioRxiv - Microbiology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
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bioRxiv - Cell Biology 2024Quote: ... and 2μg pMD2.G using TransIT (Mirus). Viral supernatant was collected 48 and 72h after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... transiently expressed in HEK293 cells using TransIT-293 transfection reagent (Mirus, Madison, WI), and characterized using whole-cell patch-clamp electrophysiology and single cell calcium microfluorimetry as described above.
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bioRxiv - Cell Biology 2024Quote: ... and either 3 µl Mirus TransIT-X2 transfection reagent (Mirus #MIR6000) for hTERT-RPE1 cells ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM NEAA and penicillin–streptomycin) were transfected with 500μl Opti-MEM containing 30 μl TransITβ-LT1 Transfection Reagent (Mirus, MIR2305), 10μg pLV-tetO-Tfap2c ...
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bioRxiv - Cell Biology 2024Quote: ... pPBhCMV1-Prdm14-pA and pPBhCMV1-Nanog-pA vectors were transfected using TransITβ-LT1 Transfection Reagent (Mirus BioTM, MIR2304) into ESCs together with pPBCAG-rtTA and pPyCAG-PBase vectors ...
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bioRxiv - Systems Biology 2024Quote: ... 8µl TransIT-293 (Mirus Bio), 1µg psPAX2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA and siRNA transfections were performed with TransIT-LT1 (Mirus Bio) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... as described previously,17 using TransIT Lenti Transfection Reagent (Mirus Bio; Cat. No. MIR 6600) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
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bioRxiv - Genomics 2024Quote: ... following the manufacturers protocol (#MIR6605, Mirus). GL261 GBM cells were transduced with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Cell Biology 2024Quote: ... TransIT-X2 (MirusBio, MIR 6000) or TransIT2020 (Mirus, MIR 5400) were used for cell transfection according to manufacturer’s instruction.
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bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were transfected using TransIT LT1 DNA transfection reagent (Mirus, Madison, WI) as described previously (Ladefoged ...
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bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: 1x 105 HeLa-GL cells/well were grown on coverslips with 500 µL medium in 24-well plates and one day later transfected with a control vector or expression vectors of SARS-CoV-2 E (Hu-1) or E T9I by using Opti-MEM reduced serum media and TransIT-LT1 Transfection Reagent (Mirus, #MIR2306) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using TransIT-LT1 transfection reagent (Mirus). Retrovirus was harvested 48 hours post-transfection from cell culture supernatant.
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bioRxiv - Evolutionary Biology 2024Quote: ... and 333 ng of pMD2.G per well) using 3 μL of Trans-IT 293T transfection reagent (Mirus Bio) per μg of DNA ...
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bioRxiv - Genetics 2024Quote: HEK293T cells were transfected with the TransIT®-LT1 transfection reagent (Mirus Bio) in 6-well plates with 1.25μg of plasmids for the CRISPR-construct and 1.25μg of plasmids for single gRNA ...
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bioRxiv - Genomics 2024Quote: ... pGYL1 contains a 318 nt mouse Alb promoter sequence derived from pLIVE plasmid (Mirus Inc., Madison, WI, #MIR 5420) [59] followed by a 1,653 nt firefly luciferase reporter sequence derived from plasmid pGL4.10 (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus expressing specific constructs was produced by transfecting HEK-293 cells in 6-well plates with a 1:1:0.1 ratio of lentiviral vector:pMD2.G:psPAX2 using the TransIT-LT1 transfection reagent (Mirus). Subsequently ...
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bioRxiv - Microbiology 2024Quote: LMH cells were seeded into 6-well plates at a density of 8.0×10⁵ and transfected with 2.5 µg of GFP-tagged cIL-4 or GFP-tagged vIL-4 using TransIT (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells at 90% conluency were transfected using TransIT (Mirus) with 7.5 µg of plasmid containing the sgRNAs and cas9 gene according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... using transIT-LT1 transfection reagent (Mirus, MIR2306). Lentivirus was harvested 48 and 72 hrs post-transfection by ultracentrifugation from cell culture supernatant (50,000 g at 4°C for 3 hours) ...
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bioRxiv - Genomics 2024Quote: ... comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [59] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... into HEK293T cells using TransIT-Lenti (Mirus Bio, Madison, WI). A 3:1 ratio of transfection reagent to total plasmid was used for all transfections ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 µg plasmid containing the appropriate transgene were used for transfection using TransIT-293 transfection reagent (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... lentiviral particles were produced by transfecting standard packaging vectors into HEK293T using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). At <24 hours post-transfection culture media with exchanged with fresh media supplemented with ViralBoost (Alstem Bio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK-293T cells were co-transfected using TransIT-Lenti transfection reagent (Mirus, USA), with 500ng psPAX2 (addgene ...
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bioRxiv - Microbiology 2024Quote: ... and lentiviral transfer plasmid pLENTI-puro using TransIT-293 Transfection reagent (Mirus). Viral harvests were conducted at 24 and 48 hours post-transfection ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were transfected with pMTBS and pCS2-ST at ∼80% confluence using TransIT-293 transfection reagent (Mirus). Cells were harvested for downstream applications 48 hours post-transfection.
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bioRxiv - Microbiology 2024Quote: Huh7.NTCP cells seeded in a 48-well plates were transfected using TransIT-LT1 transfection reagent (Mirus) and lysed 72 hours later using the Bright-Glo Luciferase reagent (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...