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Citations for Mirus Bio :
251 - 300 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell lines stably expressing dCas9-KRAB or dCas9-P300 were transfected with plasmids encoding gRNAs or lentiGuide empty vector using TransIT-X2 (Mirus Bio). RNA was extracted 2 days after transfection ...
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bioRxiv - Genetics 2024Quote: ... Transfections of wild-type and Gly1170Ser COL2A1-encoding plasmids were performed using Transit-2020 transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cambridge Institute for Medical Research) was transfected into the cells by using Trans-IT HeLaMONSTER reagent (Mirus Bio). DMSO and XXI were maintained in the culture medium during transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 293T cells were transfected with pairs of sgRNAs targeting SNORD32A and SNORD51 using TransIT-LT1 (Mirus). D3H2LN cell lines were derived from Jenkins et al32 ...
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bioRxiv - Molecular Biology 2024Quote: ... The Ramos cells were treated with different CRISPR-dCas9 (RNP) complexes using TransIT-X2 transfection reagents (Mirus Bio, USA) when cells were ∼70% confluent ...
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bioRxiv - Molecular Biology 2024Quote: ... they were transfected with the CRISPR-dCas9 (RNP) complexes using TransIT-X2 transfection reagents (Mirus Bio, USA). TransIT-X2 reagents were stored at room temperature to warm up and gently vortexed before use in order to prepare the TransIT-X2:RNP complex for transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 70% confluent) were transfected with 10 µg pMT-FLAG-Ago1 plasmid using 20 µl TransIT-Insect Transfection Reagent (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Chimeric receptors were transiently expressed in HEK Slc35A1 KO cells by transfection of plasmids with Transit-X2 dynamic delivery system (Mirus Bio) 24hrs prior to virus (Ca’09 ...
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bioRxiv - Microbiology 2024Quote: ... in serum-free DMEM and the TransIT-LT1 reagent (Mirus Bio, MIR2305). Virus was harvested and J-Lat cells were transduced with the TxLatent library at a multiplicity of infection of 0.2 ...
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bioRxiv - Molecular Biology 2024Quote: ... XLone-derived constructs were co-transfected with a plasmid encoding piggybac transposase into MCF7 cells with TransIT-LT1 Transfection Reagent (Mirus). Transfected cells were selected for blasticidin resistance for 2 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... hsPB126 was transfected with Cas9 landing pad plasmid (pHPHS800) and Bxb1 expression plasmid (pHPHS115) using TransIT-LT1 reagent (Mirus). 72 hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... using CHOgro high yield expression system (Mirus Bio, Madison, WI, USA) according to the manufacturer’s protocol and purified following the protocol by Nesspor et al ...
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bioRxiv - Neuroscience 2024Quote: ... Primary cortical neurons were electroporated using Ingenio electroporation solution (Mirus) following the manufacturer’s recommendations and the proportion 2 μg DNA:2*106 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected using TransIT-Lenti (Mirus) for 24 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... On the day of transfection 0.5 µg total DNA is diluted in 50 µL of OptiMEM per well and 1.5 µL TransIT-X2 transfection reagent (Mirus, MIR 6003). Further details about transfection conditions are provided in the Supplementary Files.
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bioRxiv - Neuroscience 2024Quote: ... All transfections were performed via lipofection using TransIt-LT1 transfection reagent (Mirus). Astrocyte progenitors were passaged into 24 well plates one day before the transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... using TransIT®-293 Transfection Reagent (Mirus, MIR 2700). 48 hr post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... the IVOC product containing all the genomic plasmids for virus particle synthesis was fully transfected into HEK293T cells (1×106 cells) with the use of TransIT-293 Transfection Reagent (Mirus). 48 hours after transfection ...
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bioRxiv - Genomics 2024Quote: ... mRNA transfections were performed using 0.5 µg of mRNA in 6-well plates using TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... with 2 μl TransIT-293 (Mirus), 0.25 μg psPAX2 (Addgene ...
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bioRxiv - Synthetic Biology 2024Quote: Fluorescence labeled IVT mRNA (Fluorescein-mRNA) was prepared using Label IT® Nucleic Acid Labeling Kit (MIR 3200, Mirus bio, USA) according to the instructions ...
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bioRxiv - Microbiology 2024Quote: ... Transfections were performed with TransIT-LT1 transfection reagent (Mirus Bio) according to manufacturer specifications ...
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bioRxiv - Microbiology 2024Quote: ... and either TransIT-LT1 transfection reagent (Mirus Bio) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... pCMVΔR8.91 and pMD2.G (5:4:1) using TransIT-293 transfection reagent (Mirus) at a 1µg DNA:2.25µl ratio ...
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bioRxiv - Genomics 2024Quote: ... using 3 µL of TransIT-LT1 Transfection Reagent (Mirus Bio) per 1 µg of DNA and Opti-MEM media ...
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bioRxiv - Genomics 2024Quote: ... myotis PKR2 per million cells using TransIT-LTI transfection reagent (Mirus Bio). The next day ...
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bioRxiv - Bioengineering 2024Quote: ... ATCC) were co-transfected with lentiviral vector plasmids pCMV-DR8.9 and pCAGGS-VSVG using TransIT-293 (Cat: MIR2704, Mirus BIO). After 17 h ...
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bioRxiv - Biochemistry 2024Quote: ... and transfected with 1.5 μg FLAG-tagged and 1.5 μg HA-tagged plasmids using TransIT-LT1 transfection reagents (Mirus Bio, Madison, WI, USA). The transfected cells were cultured for another 36 h ...
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bioRxiv - Bioengineering 2024Quote: ... and 0.3 µL of TransIT-X2 (Mirus). Genomic DNA was extracted ∼72 hours post transfection using the DNAdvance Kit (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... 0.2 µL of TransIT®-2020 transfection reagent (Mirus Bio) was diluted into 4 µL of Opti-MEM ...
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bioRxiv - Bioengineering 2024Quote: ... and TransIT-X2® Dynamic Delivery System (Mirus bio), were carried out per manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Transfections were performed using TransIT-293 Transfection Reagent (Mirus #2704) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... using TransIT-LT1 (Mirus Bio) at a 3:1 TransIT-LT1:plasmid ratio ...
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bioRxiv - Bioengineering 2024Quote: ... using TransIT-Lenti Transfection Reagent (Mirus) (3 μl of the reagent per 1 μg of total DNA) ...
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bioRxiv - Bioengineering 2024Quote: ... particles were prepared by transient transfection of HEK 293T cells with a 4:1:4 molar ratio of lentiGuide-puro plasmids:pVSVG:psPAX2 using TransIt-Lenti Transfection reagent (Mirus Bio 6603). The pVSVG and psPAX2 plasmids were gifts from Bob Weinberg (Addgene plasmid #8454 ...
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bioRxiv - Biochemistry 2024Quote: ... and 300 ng of each base editor (20 min at room temperature with 1.8 μL of TransIT-LT1 (Mirus) and 100 μL of Opti-MEM (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... using TransIT-LT1 transfection reagent (Mirus Bio). Media was changed 6-8 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus ...
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bioRxiv - Cancer Biology 2024Quote: ... and transfected with 1.5 μg FLAG-tagged full-length BRD4 and 1.5 μg V5-tagged p53Y220C plasmids using TransIT-LT1 transfection reagents (Mirus Bio, Madison, WI, USA). The transfected cells were cultured for another 48 h and treated with either compound or DMSO for 4 h before collection ...
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bioRxiv - Biophysics 2024Quote: ... or 0.4 μg dRfxCas13d-EGFP plus 0.6 μg XIST-targeting RfxCas13d crRNA plasmid using 5 μl TransIT-293 transfection reagent (Mirus Bio). After transfection ...
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bioRxiv - Biophysics 2024Quote: ... 0.8 μg cytoplasmic-targeting Csm complex plasmid and two MAP1B crRNA array plasmids (0.7 μg each) were transfected into cells using 5 μl TransIT-LT1 transfection reagent (Mirus Bio). After transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... 165 ng of packaging vector pMD2-G were combined into 7.5uL of TransIT-LT1 (Mirus Bio, MIR 2300) in 300uL Opti-MEM (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 μg of the transfer vector of interest and three volumes of TransIT-LT1 Transfection Reagent (Mirus). 48 h after transfection ...
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bioRxiv - Biophysics 2024Quote: ... Cells were grown to 60-80% confluency in 10 cm dishes and transfected using 7 µg plasmid DNA and 7 µL TransIT-Pro reagent (Mirus Bio). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... d8.9 packaging vector and VSV-G envelope vector mixed with TransIT-293 (Mirus Bio). Virus-containing supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: Plasmid constructs for the production of lentivirus were transfected with packaging plasmids psPAX2 and pMD2.G into HEK-293FT cells utilizing TransduceIT™ Transfection Reagent (Mirus Bio LLC, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were co-transfected with both plasmids using TransIt-LT1 (Mirus Bio, Madison, WI) following manufacture’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... Flp-In 293 T-REx cells were reverse co-transfected with pKI and individual pX459 plasmids using TransIT-293 (Mirus) according to the manufacturer’s instructions in media containing 690 nM Alt-R™ HDR Enhancer V2 (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... Transfections were performed using Transit LT1 reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: All transfections were performed using Transit LT1 reagent (Mirus Bio) according to the manufacturer’s protocol ...