Labshake search
Citations for Mirus Bio :
401 - 450 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Infected cells were transfected with the transfer plasmid using TransIT-LT1 (Mirus Bio, Madison, WI, USA). Recombinant viruses BeninΔA179L expressing the β-GUS gene were identified by incubation with 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid and purified by limiting dilution ...
-
bioRxiv - Molecular Biology 2023Quote: ... 7.5 µl transfection reagent TransIT-2020 (Mirus Bio) was added and incubated for 30 min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... These cells were transfected using TransIT-Lenti (Mirus Bio) using a ratio of 5:3.75:1.25 of Brunello:psPAX2:pMD2.G for a total of 10 μg per dish ...
-
bioRxiv - Cell Biology 2023Quote: ... in accordance with the manufacturer’s instructions using Ingenio electroporation solution (Mirus). Equal volumes of cell suspension were divided and plated in 3 wells of 8-well dishes (µ-Slide 8 well high glass bottom ...
-
bioRxiv - Microbiology 2023Quote: ... HeLa cells were co-transfected on a six-well plate with a 3:1 mass ratio mix of pM1-2C and pArf1-EGFP plasmids (or a plasmid expressing EGFP instead of Arf-EGFP in controls) using Mirus 2020 transfection reagent (Mirus Bio). Infected cells were processed at 6 ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfection reagents TransIT-X2® and Lipofectamine® 2000 were from Mirus Bio (Madison ...
-
bioRxiv - Cell Biology 2023Quote: ... Jurkat cells were electroporated using the Ingenio electroporation solution following the manufacturer’s instructions (Mirus 50111).
-
bioRxiv - Molecular Biology 2023Quote: ... pAC5.1 vectors of CLIP-CRYΔ and TIM- SNAP-HA-ALFA were delivered into S2 cells using the TransIT-Insect Transfection Reagent (Cat# MIR6100, Mirus) following a modified version of the manufacturer’s instruction ...
-
bioRxiv - Biophysics 2023Quote: ... the cells were plated in a 12.5 cm2 dish and were transfected at 60% confluency with 0.8 µg DNA using TransIT-293 (Mirus, MIR2700). We used a GFP-tagged and C-terminally truncated human HCN1 construct which was cloned into a pEG plasmid as previously reported by Lou & MacKinnon (2017) ...
-
bioRxiv - Genomics 2023Quote: ... and 8.25 µL TransIT-X2 (Mirus, catalog #MIR2300) suspended in a total of 250 µL of Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: Differentiated N2a cells were transfected with mRFP or syn-p2a-mRFP (1 μg) and mito GcaMP6f (1 μg) using TransIT-X2® Dynamic Delivery System (Mirus Bio) according to manufacturer’s instructions before reculturing in normal medium for 24 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... were packaged in HEK 293T cells as previously described [15] using Turbofect (Fisher, UK) or trans-IT (Mirus. USA) as lipofection reagents ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected to produce lentiviral particles with TransIT-Lenti transfection reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µg of PCR product was fluorescently labeled using the Label IT-Cy5 Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... or TransIT-X2 (Mirus) in a 6-well plate (10 μl Lipofectamine + up to 100 μl of BAC-VZV DNA) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 ng pEZY_AR plasmid and either 100 ng pEZY_eGFP or pEZY_Flag_HOXB13 using TransIT-2020 (Mirus) and then plated in DMEM (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... and TransIT-X2 (Mirus, #MIR 6000).
-
bioRxiv - Microbiology 2023Quote: ... For CPV infections, MEF cells was transfected with feline TfR1 plasmid (pCTfR, 2.5 μg)25 by using TransIT-LT1transfection reagent (Mirus Bio, WI, USA). To generate MEFDyn1,2,3 TKO cells stably expressing hACE2 ...
-
bioRxiv - Biochemistry 2023Quote: ... 48 uL TransIT-293 transfection reagent (Mirus Bio LLC, catalog no. MIR 2704) diluted with 1300 uL serum-free media (SFM ...
-
bioRxiv - Microbiology 2023Quote: ... via TransIT-X2 (Mirus). After 24 hr post-transfection ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were reverse transfected with expression plasmids via TransIT-X2 (Mirus) at a 1:3 ratio (2.5 μg plasmid ...
-
bioRxiv - Neuroscience 2023Quote: ... TransIT-LT1 (Mirus Bio, Cat. No. MIR6600) was diluted into Opti-MEM and incubated at room temperature for 5 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-Lenti Trasfection Reagent (6605, Mirus). CH157-MN cells were stably transduced with lentiviral particles to generate CH-157MN NOTCH3ICD or empty pLVX vector (EV ...
-
bioRxiv - Neuroscience 2023Quote: ... 90 μL of TransIT-LT1 (Mirus Bio, Cat. No. MIR6600) was diluted into 3 mL of Opti-MEM and incubated at RT for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... pCMV R8.74 and pCDH1-CMV-ATG3-mCherry-SV40-Hygromycin using TransIT-LT1 reagent (Mirus). After 72 hr post-transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of total plasmid DNA and 1.5 μL of TransIT ®-2020 Transfection Reagent (Mirus Bio, WI, USA) were mixed by gentle pipetting ...
-
bioRxiv - Neuroscience 2023Quote: ... and a Venus-tagged N-terminal β-arrestin2 using 3:1 ratio of TransiT-2020 (Mirus). 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced by transient transfection of Phoenix-ECO cells (CRL-3214) using TransIT®-LT1 Reagent (Mirus Bio LLC, Madison, WI, USA). The lentiCRISPR construct or the pLJM1-EGFP plasmid (Addgene plasmid #19319 ...
-
bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
-
bioRxiv - Genetics 2023Quote: ... and 305 µL of TransIT-LT1 Transfection Reagent (Mirus Bio, MIR 2300) per T175 flask ...
-
bioRxiv - Cancer Biology 2023Quote: ... The transfection was carried out with TransIT-X2 reagent (Mirus Bio.) in 6-wells ...
-
bioRxiv - Cancer Biology 2023Quote: ... using TransIT-siQUEST reagent (Mirus Bio) following manufacturer’s protocol for 48-72h ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using Transit-LT1 transfection reagent (Mirus Bio) at a 1:3 DNA to transfection reagent ratio ...
-
bioRxiv - Biophysics 2023Quote: ... 2 µg of plasmid DNA was transfected per dish using Transit 293T (Mirus) following the manufacturer’s instructions and measured at least 48 h later.
-
bioRxiv - Cancer Biology 2023Quote: Label IT Tracker (Mirus Bio, cat no. MIR7025) protocol was used to covalently attach a fluorescein-492 tag to pHPV16-LCR-luc (long control region containing transcriptional control elements ...
-
bioRxiv - Biochemistry 2023Quote: ... the cells were transfected with 1.5 μL of TransIT 2020 (Mirus) and 300 ng of plasmid DNA of interest in 50 μL of OptiMem per well ...
-
bioRxiv - Biochemistry 2023Quote: Ptch1−/− MEFs were seeded in 24-well plates and transfected using TransIT-X2® (Mirus) and Opti-MEM reduced serum media (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... psPAX2 and pMD2.G packaging constructs using TransIT-VirusGEN (Mirus). Supernatants containing high titer lentiviruses were collected at 48 and 72 hr after transfection and were filtered through a 0.45 µm cellulose acetate filters before use ...
-
bioRxiv - Immunology 2023Quote: ... mtDNA was biotinylated with Label IT Nucleic Acid Labeling Kits (Mirus) and fragmented with HaeIII (New England Biolabs ...
-
bioRxiv - Genomics 2023Quote: ... 2.1 × 107 cells were transfected 24h after plating using 510.3 µl of TransIT reagent (#MIR2300, Mirus) diluted in 18.4 ml of OPTI-MEM that was combined with 75.6 µg of each sgRNA libraries and 94.5 µg of lentiviral packaging mix (#CPCP- K2A ...
-
bioRxiv - Neuroscience 2023Quote: ... using the TransIT2020 transfection reagent (Mirus Bio). Cells were harvested 48 h post-transfection ...
-
bioRxiv - Neuroscience 2023Quote: ... TransIT-TKO (Mirus) transfection reagent was prepared adding 2.5μL to 100µl of media without serum ...
-
bioRxiv - Microbiology 2023Quote: ... siRNA transfections were performed with TransIT-X2 Transfection Kit (Mirus Bio) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 500 ng of viral genomic RNA was transfected per well in 12-well plates through the TransIT®-mRNA Transfection Kit (Mirus Bio) following manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... at a ratio of 3:1 PEI:total DNA or TransIT-LT1 (Mirus) at a ratio of 3:1 TransIT:DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1 Flag-MAVS-C79F or pcDNA3.1-Flag-MAVS-CcardA expressing constructs using TransIT-LT1 transfection reagent (Mirus) before selection using 800µg/ml geneticin (G418 ...
-
bioRxiv - Molecular Biology 2023Quote: ... before adding 6 µl of TransIT VirusGen transfection reagent (Mirus MIR6700). The transfection mixture was incubated for 10 to 15 min and then added dropwise to HEK293T cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... by using Mirus TransIT-LT1 transfection reagent (Mirus Bio LLC, cat # MIR 2305). 24 h post-transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... into Hek293T cells (ATCC) using transIT®-LT1 (Mirus Bio). The growth media was replenished one day later ...
-
bioRxiv - Molecular Biology 2023Quote: ... 250 ng of each plasmid construct (Empty vector, DDI2 WT, DDI D252, NFE2L1 WT, and NFE2L1-8ND) was added to TransIT-X2® Transfection Reagent (Mirus) and after 10 min incubation ...