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Citations for Mirus Bio :
401 - 450 of 1473 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 6-well dishes and transfected with mEGFP-tagged DNAJC17 or deletion mutants using TransIt-LT1 reagent at a 3:1 ratio (Mirus). Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek ...
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bioRxiv - Cancer Biology 2023Quote: ... pCMV-dR8.91 and a transfer plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) and 8 ng/uL polybrene ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...
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bioRxiv - Microbiology 2023Quote: ... 3μL of TransIT-2020 (Mirus Bio, Madison, WI) was added and the reactions were incubated for 30 minutes prior to addition to the cells in the BSL-3 ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Biophysics 2023Quote: ... and 100 ng of the plasmid of interest were transiently co-transfected using 1.875 µL TransIT (Mirus Bio), and Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA mixtures were mixed with TransIT-X2 (Mirus) at a ratio of 0.5 µL of TransIT-X2 per 100 ng of total DNA ...
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bioRxiv - Bioengineering 2023Quote: ... TransIT-LT1 (Mirus Bio) was used as transfection reagent according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cell line was used for retroviral and lentiviral system amplification with TransIT-293 Transfection Reagent (Mirus).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using TransIT-Lenti Transfection Reagent (Mirus Bio, Madison, WI). A 3:1 ratio of transfection reagent to total plasmid was used for all transfections ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors were produced in HEK-293T cells by co-transfection of the previously sequenced vector constructs with psPAX and pMDG.2 packaging plasmids using Trans-IT LT-1 (Mirus, MIR2700). HEK293T cells were incubated overnight at 37°C and the next day medium was changed for IMDM 10% HI-FBS ...
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bioRxiv - Cell Biology 2023Quote: ... All transfections were performed using TransIT-2020 transfection reagent (Mirus) according to the supplier’s manual ...
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bioRxiv - Genetics 2023Quote: ... using the TransIT Lentivirus transfection reagent (MIR 6600, Mirus Bio, Madison, WI, USA). gRNA libraries were designed from Brunello gRNAs in seven sub-pools with 4-gRNAs per gene and 5% Non-Target (Table S1) ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus were produced with HEK293T cells and Mirus TransIT Lentivirus System (Mirus, 6650) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed with 2 × SSC three times and then the nucleic acids in the samples were amine-modified using a Label IT Amine modifying kit (Mirus Bio) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: 293T cells were transfected using Trans IT®-293 (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Stable chemokine receptor-expressing cells (WT CCR5 and CCR5-APEX) were generated by transfecting HEK293T with pcDNA3.1 Hygro+ expression vectors using the TransIT-LT1 transfection reagent (Mirus Bio, MIR2305). Stable transfectants were subsequently selected using 100 ug/mL Hygromycin B (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell lines stably expressing dCas9-KRAB or dCas9-P300 were transfected with plasmids encoding gRNAs or lentiGuide empty vector using TransIT-X2 (Mirus Bio). RNA was extracted 2 days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... TransIT-X2® Dynamic Delivery System (Mirus) was used for transfection according to manufacturers’ instructions.
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bioRxiv - Microbiology 2023Quote: ... Total of 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus) in 250 μL Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Immunology 2023Quote: ... 16 hpt using TransIT X2 (Mirus) transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Cancer Biology 2023Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... At 30% confluence cell were transfected with siRNAs at final concentrations of 20 nM using transfection agent TransIT-X2® Dynamic Delivery System (Mirus). Cells were lysed for western blot and/or fixed for confocal microscopy analysis and images acquisition ...
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HEK-293T cells were co-transfected using TransIT-Lenti transfection reagent (Mirus, USA), with 500ng psPAX2 (addgene ...
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bioRxiv - Immunology 2023Quote: ... or transfected using TransIT-LT1 (Mirus Bio, USA) with HT-DNA (5 µg/ml) ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were performed using TransIT X2 (Mirus, MIR6003) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... TransIT-X2® transfection reagent was purchased from Mirus (Cat #6000) and used as received.
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bioRxiv - Biophysics 2023Quote: COS-7 cells were transfected with Trans-IT LT1 (Mirus) according to the manufacturer’s instructions and collected 48 h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... MCF10A cells were treated with an additional 750 μL of medium containing 3 μM puromycin (Mirus Bio 5940), achieving a final concentration of 1 μM (0.5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid constructs was performed using Trans-IT 2020 reagent (Mirus Bio, #MIR 5400) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug plasmid DNA and 3 uL TransIT-LT1 transfection reagent (Mirus, Cat#2300) were diluted in 100 uL Opti-MEM Reduced-Serum Medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... MEFs were nucleofected using Ingenio Solution electroporation reagent (Mirus Bio) and Lonza AMAXA Nucleofector II ...
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bioRxiv - Cell Biology 2023Quote: ... This mixture was incubated with the TransIT-293 Transfection Reagent (Mirus Bio) and used to transfect the 293T cells following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Transfection was performed using TransIT-LT1 (Mirus) transfection reagent according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with 1.5 μl X2 Transfection Reagent (Mirus) and 200ng plasmid DNA (pEGFP-C2 containing an insert derived from CIZ1) ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently overexpressed using TransIT-LT1 (Mirus, MIR2306) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... TransIT (Mirus) was used to transfect the plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... 48 μl of TransIT-LT1 (Mirus Bio LLC) reagent was mixed into 1.3 ml Opti-MEMTM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells were transiently transfected using TransIT-LT1 (Mirus #MIR2306) according to the manufacturer’s protocol at a ratio of 3µL of transfection reagent per 1µg of DNA and the medium was replaced 24 hours post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day (Day 0) plated cells were transfected with the TransIT-LT1 Transfection Reagent (Mirus #2306), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... TransIT-X2 Dynamic Delivery system (Mirus) was used for transfection following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... TransIT-LT1 (Mirus Bio) was added dropwise ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2.5x105 wildtype K562 cells and 2.5x105 reporter K562 cells were mixed for a total of 5.0x105 cells per well and transfected with various amounts of the purified mRNA using the TransIT®-mRNA Transfection Kit (Mirus Bio, catalog# MIR2250). To induce the reporter cell lines ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared cells were co-transfected using the TransIT®-Lenti transfection reagent (Mirus Bio) with MISSION® Genomics Lentivirus Packaging Mix (Mirus Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... with MISSION® Genomics Lentivirus Packaging Mix (Mirus Bio) and lentiviral plasmids containing i53 variants/libraries of interest ...