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Citations for Mirus Bio :
1 - 50 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the barcoded viral genomes were transfected into 293T cells using TransIT-LT1 (Mirus Bio, Madison, WI), and incubated for 48 h at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... 8µl TransIT-293 (Mirus Bio), 1µg psPAX2 ...
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bioRxiv - Immunology 2024Quote: ... or complexes of polymeric nanoparticles (TRANS-ITX2-Mirus bionova) containing the same concentration of Poly I:C (Nano-PIC) ...
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bioRxiv - Genomics 2024Quote: ... following the manufacturers protocol (#MIR6605, Mirus). GL261 GBM cells were transduced with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM NEAA and penicillin–streptomycin) were transfected with 500μl Opti-MEM containing 30 μl TransITβ-LT1 Transfection Reagent (Mirus, MIR2305), 10μg pLV-tetO-Tfap2c ...
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bioRxiv - Cell Biology 2024Quote: ... pPBhCMV1-Prdm14-pA and pPBhCMV1-Nanog-pA vectors were transfected using TransITβ-LT1 Transfection Reagent (Mirus BioTM, MIR2304) into ESCs together with pPBCAG-rtTA and pPyCAG-PBase vectors ...
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bioRxiv - Cell Biology 2024Quote: ... and either 3 µl Mirus TransIT-X2 transfection reagent (Mirus #MIR6000) for hTERT-RPE1 cells ...
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bioRxiv - Neuroscience 2024Quote: ... transiently expressed in HEK293 cells using TransIT-293 transfection reagent (Mirus, Madison, WI), and characterized using whole-cell patch-clamp electrophysiology and single cell calcium microfluorimetry as described above.
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bioRxiv - Microbiology 2024Quote: ... 80% confluent monolayers of Vero E6 cells in 12-well plates were transfected with 1.0 μg per well of infectious SARS-CoV-2 BAC DNA or its mutated derivatives using TransIT®-LT1 transfection reagent according to manufacturer’s instructions (Mirus Bio). At 48 h post transfection ...
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bioRxiv - Microbiology 2024Quote: ... Transfection experiments were conducted using TransIT-LT1 reagent (Mirus Bio LLC, Madison, WI, USA). After five days of incubation ...
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bioRxiv - Microbiology 2024Quote: ... using TransIT-LT1 (Mirus Bio LLC) and incubated at 37 °C for 30 h ...
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bioRxiv - Cell Biology 2024Quote: ... cells were harvested and resuspended in 100μL of Ingenio® EZporator® Electroporation Solution (Mirus Bio). Ligand was added (2μg LPS for epithelial cells ...
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bioRxiv - Molecular Biology 2024Quote: ... the transfer plasmid and helper plasmids VSV-G and PSP (generously provided by David Sanders) were transfected into the Lenti-X cells using Transit293 transfection reagent (Mirus, Cat. No. MIR 2700) and incubated in OptiMEM (ThermoFisher Cat No ...
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bioRxiv - Developmental Biology 2024Quote: ... and 10μg vectors (from pLX_311 lentiviral expression of dCas9-KRAB and the entire constructed pXPR_050 CRISPRi sgRNA expression library) using TransIT-LT1 (Mirus; 2305). HEK293T culture media was collected at both 48 and 72 hours post transfection and concentrated using Amicon Ultra-15 columns (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... NIH-3T3 fibroblasts and C2C12 myoblasts were transfected using TransIT-X2 (Mirus Bio LLC) and HEK-293 cells with ScreenFect-A (ScreenFect ...
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bioRxiv - Molecular Biology 2024Quote: ... or Transit-2020 (Mirus Bio LLC; iron export experiments), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The T25D-Geminin stable cell line was generated by transfecting (Mirus Bio) with two plasmids ...
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bioRxiv - Molecular Biology 2024Quote: ... 400’000 cells were transfected for 24h using Lipofectamine 300 (Mirus Bio, ref. MIR2300) and 2µg of sgRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were transfected into the KOLF2_C1 hiPSC line with TransIT-LT1 (Mirus Bio) and editing efficiencies measured at day 4 post-transfection by flow cytometry (gating on GFP positive cells ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were resuspended in nucleofector solution (Mirus) and transfected using an Amaxa/Lonza Nucleofector II ...
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bioRxiv - Microbiology 2024Quote: iSLK.219 cells were maintained as previously described and were transfected with TransIT-X2 (Mirus MIR6004) according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2024Quote: ... cells in one 35 mm dish were either transiently transfected with the construct to be imaged using TransIT-293 lipofection reagent (Mirus Bio) or were virally transduced with a lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected into 293T/17 cells (P27, ATCC) using TransIT-Lenti Transfection Reagent (Mirus). Cells ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells seeded in a 10 cm dish were transfected using TransIT-293 (Mirus Bio, Madison, WI, USA) with 670 ng pCAGGS-GP ...
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bioRxiv - Microbiology 2024Quote: ... and a CMV-Renilla plasmid using Mirus Transit X2 (Mirus MIR6004). At 48 hours post-transfection ...
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bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
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bioRxiv - Immunology 2024Quote: ... Immortalized Hoil1f/f MEFs were transiently transfected with a plasmid expressing Cre-recombinase (pTrip-CMV-Cre-Puro-T2A-RFP) using TransIT-LT1 (Mirus), and Hoil1-/- and Hoil1f/f single-cell clones isolated and identified by PCR ...
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA and siRNA transfections were performed with TransIT-LT1 (Mirus Bio) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... as described previously,17 using TransIT Lenti Transfection Reagent (Mirus Bio; Cat. No. MIR 6600) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2024Quote: ... This sgRNA-SpCas9 complex solution was then combined with TransIT-LT1 reagent (Mirus Bio) and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... TransIT LTI (Mirus Bio) was used to transfect 30ng of either IFNβ promoter or ISRE promoter-firefly luciferase reporters and 30ng pRL-TK Renilla luciferase ...
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bioRxiv - Microbiology 2024Quote: ... A ratio of 1ug DNA to 3ul TransIT LTI (Mirus Bio, Madison, WI, USA) was used to transfect cells with 20.8ng VP35 ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... cells with successful integration were selected by addition of 100 ug/ml Hygromycin (Mirus Bio, MIR5930) for 4 days ...
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bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
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bioRxiv - Immunology 2024Quote: ... and 6 µl of Transit-LT1 (Mirus #MIR2300). Two days after transfection lentivirus was harvested and mixed with equal parts fresh media before overlaying on top of target cell lines ...
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bioRxiv - Cell Biology 2024Quote: ... and 2μg pMD2.G using TransIT (Mirus). Viral supernatant was collected 48 and 72h after transfection ...
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bioRxiv - Cell Biology 2024Quote: Transfection of Capsaspora cells and stable cell line generation was done using the TransIT-X2 transfection reagent (Mirus Bio) as previously described (Phillips et al. ...
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bioRxiv - Microbiology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl Mirus TransIT-LT1 transfection reagent (Mirus #MIR2300), and up to 1 µg of plasmid DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were co-transfected (Mirus LT1 or Mirus X2) with uniform masses of dCasMINI-modulator- and sgRNA-expressing plasmids such that each well received a single modulator construct to be tested ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were co-transfected (Mirus LT1 or Mirus X2) with uniform masses of dCasMINI-modulator- and sgRNA-expressing plasmids such that each well received a single modulator construct to be tested ...
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bioRxiv - Immunology 2024Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7Lμg of the vector ...
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bioRxiv - Physiology 2024Quote: ... Cell transfections were done using TransIT-X2 (Mirus) according to the manufacturer’s protocol with a 3:1 ratio of transfection agent to DNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 16 μL TransIT®-LT1 (Mirus) and 1 μg for each of the 8 plasmids encoding the corresponding virus fragments were mixed thoroughly for transfection ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Molecular Biology 2024Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (35) ...