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Citations for Mirus Bio :
1 - 50 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 6 µl of Transit-LT1 (Mirus #MIR2300). Two days after transfection lentivirus was harvested and mixed with equal parts fresh media before overlaying on top of target cell lines ...
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bioRxiv - Immunology 2024Quote: ... using TransIT-293 transfection reagent (Mirus Bio Cat. 2705). Media was changed 16 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 μL Ingenio Electroporation Solution (Mirus - MIR50111). Samples were electroporated using the D-23 program on an Amaxa Nucleofector ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... cells with successful integration were selected by addition of 100 ug/ml Hygromycin (Mirus Bio, MIR5930) for 4 days ...
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bioRxiv - Microbiology 2024Quote: ... Transfection experiments were conducted using TransIT-LT1 reagent (Mirus Bio LLC, Madison, WI, USA). After five days of incubation ...
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bioRxiv - Microbiology 2024Quote: ... using TransIT-LT1 (Mirus Bio LLC) and incubated at 37 °C for 30 h ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected into 293T/17 cells (P27, ATCC) using TransIT-Lenti Transfection Reagent (Mirus). Cells ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells seeded in a 10 cm dish were transfected using TransIT-293 (Mirus Bio, Madison, WI, USA) with 670 ng pCAGGS-GP ...
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bioRxiv - Cell Biology 2024Quote: ... cells were harvested and resuspended in 100μL of Ingenio® EZporator® Electroporation Solution (Mirus Bio). Ligand was added (2μg LPS for epithelial cells ...
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bioRxiv - Molecular Biology 2024Quote: ... or Transit-2020 (Mirus Bio LLC; iron export experiments), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... NIH-3T3 fibroblasts and C2C12 myoblasts were transfected using TransIT-X2 (Mirus Bio LLC) and HEK-293 cells with ScreenFect-A (ScreenFect ...
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bioRxiv - Physiology 2024Quote: ... Cell transfections were done using TransIT-X2 (Mirus) according to the manufacturer’s protocol with a 3:1 ratio of transfection agent to DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl Mirus TransIT-LT1 transfection reagent (Mirus #MIR2300), and up to 1 µg of plasmid DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were co-transfected (Mirus LT1 or Mirus X2) with uniform masses of dCasMINI-modulator- and sgRNA-expressing plasmids such that each well received a single modulator construct to be tested ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were co-transfected (Mirus LT1 or Mirus X2) with uniform masses of dCasMINI-modulator- and sgRNA-expressing plasmids such that each well received a single modulator construct to be tested ...
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bioRxiv - Biophysics 2024Quote: ... Lentiviral particles containing each plasmid were produced by transfecting standard packaging vectors along with the plasmids into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). Media was changed at 24 hours post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected using TransIT-2020 Transfection Reagent (Mirus Bio) with 1 ug of EFHD1 cDNA and 0.5 µg of mt-AEQ or cytosolic aequorin ...
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bioRxiv - Bioengineering 2024Quote: ... Cell transfection was performed using the TransIT-Lenti transfection reagent (Mirus bio, Madison, WI) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 at a ratio of 1:1:0.1 using TransIT-LT1 transfection reagent (Mirus). The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 16 μL TransIT®-LT1 (Mirus) and 1 μg for each of the 8 plasmids encoding the corresponding virus fragments were mixed thoroughly for transfection ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Molecular Biology 2024Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (35) ...
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bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Transient transfections were carried using TransIT-LT1 reagent (Mirus Bio). HEK293T ...
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bioRxiv - Biochemistry 2024Quote: ... they were transfected with 1800 ng of plasmid containing either N- or C-terminal tagged GFP-AMPKγ3 using TransITx2 transfection reagent (Mirus Bio, cat no. MIR 6000) and then lysed 48 hours later (lysis buffer – 50 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... using a TransIT-X2 transfection kit (Mirus). Tether radius was obtained by comparing tether fluorescence to fluorescence counts from a known area of the parent cell membrane ...
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bioRxiv - Cell Biology 2024Quote: ... and PEX19 sgRNAs were transfected in 96-well plates by lipofectamine (TransIT LT-1, Mirus). A normalized 55 ng of total plasmid DNA was used at a ratio of 50:5 TOPFlash/FOPFlash:Renilla ...
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bioRxiv - Cell Biology 2024Quote: ... using TransIT-2020 (Mirus, MIR 5400). The cells were passaged when needed ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight and transfected after 24 hours with x2 lipid (Mirus Bio) and either 25 nM control (Horizon ...
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bioRxiv - Cancer Biology 2024Quote: ... using the SiQuest (Mirus, TransIT-siQUEST®) using previously described procedure ...
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bioRxiv - Cancer Biology 2024Quote: ... 22 µL of Mirus LT1 transfection reagent (Mirus) and 4 µg of lentiviral plasmids of packaging vector (pPAX ...
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bioRxiv - Cell Biology 2024Quote: ... Generation of lentivirus was performed by transfecting HEK293T cells with standard delta VPR and VSVG packaging vectors paired with TransIT-LTI Transfection Reagent (MIR2305, Mirus). Lentivirus was harvested 72 hrs following transfection and frozen at -80°C.
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bioRxiv - Microbiology 2024Quote: ... 80% confluent monolayers of Vero E6 cells in 12-well plates were transfected with 1.0 μg per well of infectious SARS-CoV-2 BAC DNA or its mutated derivatives using TransIT®-LT1 transfection reagent according to manufacturer’s instructions (Mirus Bio). At 48 h post transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... and 10μg vectors (from pLX_311 lentiviral expression of dCas9-KRAB and the entire constructed pXPR_050 CRISPRi sgRNA expression library) using TransIT-LT1 (Mirus; 2305). HEK293T culture media was collected at both 48 and 72 hours post transfection and concentrated using Amicon Ultra-15 columns (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... the transfer plasmid and helper plasmids VSV-G and PSP (generously provided by David Sanders) were transfected into the Lenti-X cells using Transit293 transfection reagent (Mirus, Cat. No. MIR 2700) and incubated in OptiMEM (ThermoFisher Cat No ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the barcoded viral genomes were transfected into 293T cells using TransIT-LT1 (Mirus Bio, Madison, WI), and incubated for 48 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 400’000 cells were transfected for 24h using Lipofectamine 300 (Mirus Bio, ref. MIR2300) and 2µg of sgRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were transfected into the KOLF2_C1 hiPSC line with TransIT-LT1 (Mirus Bio) and editing efficiencies measured at day 4 post-transfection by flow cytometry (gating on GFP positive cells ...
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bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
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bioRxiv - Immunology 2024Quote: ... Immortalized Hoil1f/f MEFs were transiently transfected with a plasmid expressing Cre-recombinase (pTrip-CMV-Cre-Puro-T2A-RFP) using TransIT-LT1 (Mirus), and Hoil1-/- and Hoil1f/f single-cell clones isolated and identified by PCR ...
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bioRxiv - Genomics 2024Quote: ... The T25D-Geminin stable cell line was generated by transfecting (Mirus Bio) with two plasmids ...
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bioRxiv - Plant Biology 2024Quote: ... This sgRNA-SpCas9 complex solution was then combined with TransIT-LT1 reagent (Mirus Bio) and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: iSLK.219 cells were maintained as previously described and were transfected with TransIT-X2 (Mirus MIR6004) according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were resuspended in nucleofector solution (Mirus) and transfected using an Amaxa/Lonza Nucleofector II ...
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bioRxiv - Immunology 2024Quote: ... or complexes of polymeric nanoparticles (TRANS-ITX2-Mirus bionova) containing the same concentration of Poly I:C (Nano-PIC) ...
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bioRxiv - Cell Biology 2024Quote: ... 24h later cells were transfected with TransIT-2020 (Mirus, MIR 5404) as previously described (Nawara et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Clonal cell lines expressing GFP/biotin ligase fusions were generated using TransIT transfection reagent (Mirus) by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen) ...