Labshake search
Citations for Mirus Bio :
1 - 50 of 1473 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... 24h later cells were transfected with TransIT-2020 (Mirus, MIR 5404) as previously described (Nawara et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... Clonal cell lines expressing GFP/biotin ligase fusions were generated using TransIT transfection reagent (Mirus) by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen) ...
-
bioRxiv - Genomics 2024Quote: ... and TransIT-LT1 (Mirus MIR 2300) transfection reagent ...
-
bioRxiv - Genetics 2024Quote: ... using TransIT-LT1 (Mirus) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 0.2µl TransIT-X2 Dynamic Delivery System (Mirus, 6003) and supplement-free DMEM and incubated at RT for 20mins ...
-
bioRxiv - Immunology 2024Quote: ... LT1 transfection reagent (Mirus Bio, Madison, WI), and the pCL-ECO packaging plasmid ...
-
bioRxiv - Microbiology 2024Quote: ... and L segments using the TransIT-LT1transfection reagent (Mirus Bio) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... using TransIT-LT1 (Mirus). Supernatants were collected at 24 and 48 h ...
-
bioRxiv - Microbiology 2024Quote: ... pTM-P and 1 pTM-L using TransIT-LT1 (Mirus) and incubated at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... using TransIT-293 transfection reagent (Mirus Bio Cat. 2705). Media was changed 16 hours post-transfection ...
-
bioRxiv - Immunology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
-
bioRxiv - Cell Biology 2024Quote: ... and PEX19 sgRNAs were transfected in 96-well plates by lipofectamine (TransIT LT-1, Mirus). A normalized 55 ng of total plasmid DNA was used at a ratio of 50:5 TOPFlash/FOPFlash:Renilla ...
-
bioRxiv - Cell Biology 2024Quote: ... TransIT-X2 (MirusBio, MIR 6000) or TransIT2020 (Mirus, MIR 5400) were used for cell transfection according to manufacturer’s instruction.
-
bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were transfected using TransIT LT1 DNA transfection reagent (Mirus, Madison, WI) as described previously (Ladefoged ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cy5-labeled probes were generated from RP11-440N18 BAC DNA sonicated to 150 bp and labeled using a DNA labeling kit (Mirus Bio). Slides were denatured with 0.5 M NaOH for 30 min at room temperature ...
-
bioRxiv - Microbiology 2024Quote: Plasmids encoding the barcoded viral genomes were transfected into 293T cells using TransIT-LT1 (Mirus Bio, Madison, WI), and incubated for 48 h at 37°C ...
-
bioRxiv - Systems Biology 2024Quote: ... 8µl TransIT-293 (Mirus Bio), 1µg psPAX2 ...
-
bioRxiv - Immunology 2024Quote: ... or complexes of polymeric nanoparticles (TRANS-ITX2-Mirus bionova) containing the same concentration of Poly I:C (Nano-PIC) ...
-
bioRxiv - Genomics 2024Quote: ... following the manufacturers protocol (#MIR6605, Mirus). GL261 GBM cells were transduced with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
-
bioRxiv - Cell Biology 2024Quote: ... The human AD293 and iPSC cell lines were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat # MIR 2300, Mirus Bio LLC, Madison, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... 1mM NEAA and penicillin–streptomycin) were transfected with 500μl Opti-MEM containing 30 μl TransITβ-LT1 Transfection Reagent (Mirus, MIR2305), 10μg pLV-tetO-Tfap2c ...
-
bioRxiv - Cell Biology 2024Quote: ... pPBhCMV1-Prdm14-pA and pPBhCMV1-Nanog-pA vectors were transfected using TransITβ-LT1 Transfection Reagent (Mirus BioTM, MIR2304) into ESCs together with pPBCAG-rtTA and pPyCAG-PBase vectors ...
-
bioRxiv - Cell Biology 2024Quote: ... and either 3 µl Mirus TransIT-X2 transfection reagent (Mirus #MIR6000) for hTERT-RPE1 cells ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were cultured in T175 cm2 plates to 85–90% confluency and transfected with the target vectors (10 µg/plate) using TransIT-X2® dynamic delivery transfection reagent (#MIR6006, Mirus). After 48 hours post-transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... transiently expressed in HEK293 cells using TransIT-293 transfection reagent (Mirus, Madison, WI), and characterized using whole-cell patch-clamp electrophysiology and single cell calcium microfluorimetry as described above.
-
bioRxiv - Microbiology 2024Quote: ... 80% confluent monolayers of Vero E6 cells in 12-well plates were transfected with 1.0 μg per well of infectious SARS-CoV-2 BAC DNA or its mutated derivatives using TransIT®-LT1 transfection reagent according to manufacturer’s instructions (Mirus Bio). At 48 h post transfection ...
-
bioRxiv - Microbiology 2024Quote: ... Transfection experiments were conducted using TransIT-LT1 reagent (Mirus Bio LLC, Madison, WI, USA). After five days of incubation ...
-
bioRxiv - Microbiology 2024Quote: ... using TransIT-LT1 (Mirus Bio LLC) and incubated at 37 °C for 30 h ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were harvested and resuspended in 100μL of Ingenio® EZporator® Electroporation Solution (Mirus Bio). Ligand was added (2μg LPS for epithelial cells ...
-
bioRxiv - Molecular Biology 2024Quote: ... the transfer plasmid and helper plasmids VSV-G and PSP (generously provided by David Sanders) were transfected into the Lenti-X cells using Transit293 transfection reagent (Mirus, Cat. No. MIR 2700) and incubated in OptiMEM (ThermoFisher Cat No ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 10μg vectors (from pLX_311 lentiviral expression of dCas9-KRAB and the entire constructed pXPR_050 CRISPRi sgRNA expression library) using TransIT-LT1 (Mirus; 2305). HEK293T culture media was collected at both 48 and 72 hours post transfection and concentrated using Amicon Ultra-15 columns (Millipore ...
-
bioRxiv - Molecular Biology 2024Quote: ... NIH-3T3 fibroblasts and C2C12 myoblasts were transfected using TransIT-X2 (Mirus Bio LLC) and HEK-293 cells with ScreenFect-A (ScreenFect ...
-
bioRxiv - Molecular Biology 2024Quote: ... or Transit-2020 (Mirus Bio LLC; iron export experiments), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... The T25D-Geminin stable cell line was generated by transfecting (Mirus Bio) with two plasmids ...
-
bioRxiv - Molecular Biology 2024Quote: ... 400’000 cells were transfected for 24h using Lipofectamine 300 (Mirus Bio, ref. MIR2300) and 2µg of sgRNAs ...
-
bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
-
bioRxiv - Molecular Biology 2024Quote: ... Plasmids were transfected into the KOLF2_C1 hiPSC line with TransIT-LT1 (Mirus Bio) and editing efficiencies measured at day 4 post-transfection by flow cytometry (gating on GFP positive cells ...
-
bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were resuspended in nucleofector solution (Mirus) and transfected using an Amaxa/Lonza Nucleofector II ...
-
bioRxiv - Microbiology 2024Quote: iSLK.219 cells were maintained as previously described and were transfected with TransIT-X2 (Mirus MIR6004) according to the manufacturer’s specifications ...
-
bioRxiv - Neuroscience 2024Quote: ... cells in one 35 mm dish were either transiently transfected with the construct to be imaged using TransIT-293 lipofection reagent (Mirus Bio) or were virally transduced with a lentivirus ...
-
bioRxiv - Cell Biology 2024Quote: ... as for HEK293T using TransIT-293 Transfection Reagent (MIR2704, Mirus), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... were transfected into 293T/17 cells (P27, ATCC) using TransIT-Lenti Transfection Reagent (Mirus). Cells ...
-
bioRxiv - Microbiology 2024Quote: HEK293T cells seeded in a 10 cm dish were transfected using TransIT-293 (Mirus Bio, Madison, WI, USA) with 670 ng pCAGGS-GP ...
-
bioRxiv - Microbiology 2024Quote: ... and a CMV-Renilla plasmid using Mirus Transit X2 (Mirus MIR6004). At 48 hours post-transfection ...
-
bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
-
bioRxiv - Immunology 2024Quote: ... Immortalized Hoil1f/f MEFs were transiently transfected with a plasmid expressing Cre-recombinase (pTrip-CMV-Cre-Puro-T2A-RFP) using TransIT-LT1 (Mirus), and Hoil1-/- and Hoil1f/f single-cell clones isolated and identified by PCR ...
-
bioRxiv - Neuroscience 2024Quote: ... and maintained in the media described above supplemented with either 1.5ug/mL (HEK-293T and HeLa) or 10µg/mL puromycin (MDCK) (Mirus Bio, MIR 5940). A clonal MDCK-ALPL line with high transcytosis activity (MDCK-ALPL clone 21 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA and siRNA transfections were performed with TransIT-LT1 (Mirus Bio) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... as described previously,17 using TransIT Lenti Transfection Reagent (Mirus Bio; Cat. No. MIR 6600) according to manufacturer’s protocol.
-
bioRxiv - Plant Biology 2024Quote: ... This sgRNA-SpCas9 complex solution was then combined with TransIT-LT1 reagent (Mirus Bio) and incubated at room temperature for 5 minutes ...