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Citations for Mirus Bio :
301 - 350 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 1.5 µg of the sgRNA plasmid were cotransfected into the cell line using TransIT transfection reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then transfected upon reaching 60% confluency using TransIT transfection reagents (Mirus). The images were taken 24 hours post-transfection.
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bioRxiv - Cell Biology 2024Quote: ... Transfections of wild-type and Arg719Cys COL2A1-encoding plasmids were performed using Transit-2020 transfection reagent (Mirus), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... into HEK293T cells using TransIT-Lenti transfection reagent (Mirus Bio) or calcium phosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... and 9μg transfer plasmid with 36μL of TransIT Leti reagent (Mirus MIR 6604). Cells were changed to fresh media the next day and viral media was collected on day 3 for concentration with Lenti-X concentrator (Takara 631232) ...
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bioRxiv - Cancer Biology 2024Quote: ... Mirus LT1 transfection reagent was purchased from Mirus company (Life Technology ...
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bioRxiv - Cell Biology 2024Quote: ... pCMV or pGFP using TransIT-2020 (Mirus) transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: Retrovirus was produced by transfecting Platinum-E (Plat-E) cells with pBMN plasmids using TransIT-LT1 (Mirus) according to the manufacturer’s standard instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection was carried out using TransIT®-LT1 (Mirus) or Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... using Trans IT-Virus GEN transfection Reagent (Mirus). Lentiviral supernatants were harvested ...
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bioRxiv - Cell Biology 2024Quote: Early passage HEK293T cells were used to package lentivirus: they were transfected using Transit Lenti transfection reagent (Mirus Bio) and Opti-MEM (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2μg pMD2.G using TransIT (Mirus). Viral supernatant was collected 48 and 72h after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus ...
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bioRxiv - Biochemistry 2024Quote: ... using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus ...
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bioRxiv - Biochemistry 2024Quote: ... and then transfected with 1.5 μg FLAG-tagged BRD4 plasmid using TransIT-LT1 transfection reagents (Mirus Bio, Madison, WI, USA). The transfected cells were cultured for another 36 h ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells at 80% confluence were transiently transfected with the plasmids encoding FLAG-tagged TFEB or TFE3 using TransIT-X2 Dynamic Delivery System (Mirus, MIR6000). The next day after plating the cells in 6-well plates ...
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bioRxiv - Bioengineering 2024Quote: ... which included a supplemented weight of a stuffer plasmid BPK1098 to reach 100 ng total DNA) mixed with 0.72 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... and 12.5 ng ngRNA plasmid mixed with 0.79 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Lentivirus was produced by transfecting HEK293T with pLVX lentiviral plasmid containing RUSH constructs and standard packaging vectors using TransIT-LTI Transfection Reagent (Mirus, MIR 2306). Viral particles in the supernatant was harvested at 2 days after transfection and stored frozen at -80°C until used for infection.
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bioRxiv - Cell Biology 2024Quote: ... cells were split 1:2 onto pre-coated 12-well plates and 4 h later were transfected with 500 ng ERGFP_NS using TransIT-293 Transfection Reagent (Mirus). Media was changed after 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells grown in four 10cm dishes were transfected with 6 µg CAT-tail mimetic DNA using TransIT293 (Mirus Bio). Cells were expanded for 72 h and then harvested in ice cold PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from passages 15 to 35 were utilized and seeded at 250,000 cells per 6-well and transfected 24 hours after plating with plasmids using TransIT-2020 (#MIR 5404, Mirus Bio) and Opti-MEMTM (#31985070 ...
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bioRxiv - Bioengineering 2024Quote: ... the phage particles were heated-inactivated by immersing them in water at 96 °C for 10 minutes and subsequently mixed with TransIT-X2 (0.2 µL/well, Mirus Bio). Following overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... TransIT-LT2 transfection reagent (Mirus Bio, Madison, WI) was used for all transient transfection according to manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... the lentiviral p53 target gene sgRNA library was transfected into 293AH cells with TransIT®-LT1 Transfection Reagent (Mirus, MIR2304). 48 hours later lentivirus was transduced into target cells ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected (Mirus Trans-IT) with 1.6μg of lentiviral vector ...
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bioRxiv - Cancer Biology 2024Quote: ... OptiMEM and TransIT-293 Transfection Reagent (Mirus, MIR2700) were used following manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.3µL of the TransIT®-LT1 transfection reagent (Mirus, MIR2305) was added before the mix was incubated for 15 minutes in a 96-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-cultured with 15 μL of TransIT-LT1 (Mirus Bio) reagent and 5 μg of plasmids comprising sgRNA and packaging vectors PLP1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and transfer plasmid at equimolar ratios using TransIT-293 transfection reagent (Mirus Bio, Cat#MIR2704) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: MDA-MB-231 cells were transfected using TransIT®-BrCa Transfection reagent (Mirus) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells were transfected using TransIT®-293 Transfection reagent (Mirus) for the generation of retrovirus and lentivirus.
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were transfected with HA-CXCR4 using TransIT-LT1 transfection reagents (Mirus Bio, Madison, WI) as previously described [79].
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bioRxiv - Bioengineering 2024Quote: ... The transfection solution was prepared using Trans-IT (MIR2304, Mirus Bio) as described by the manufacturer using a total of 15 µg of pLenti-Lifeact-GFP ...
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bioRxiv - Bioengineering 2024Quote: ... Transfections were performed using Transit LT1 reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: All transfections were performed using Transit LT1 reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into cells using siQuest (Mirus; Cat# 2114). The cells were transfected with the siRNA for 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were cultured in a 6-well plate and transfected at 50% confluence with 2μg of px330 plasmids (and 1μl of 10μM single stranded donor oligo when applicable) using Mirus TransIT-293 Transfection reagent (Mirus, MIR2705). 48h post transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... in a ratio (4:3:1) using TransIT®-LT1Transfection reagent (MIR 2304, Mirus Bio) as described by manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... which included a supplemented weight of a stuffer plasmid BPK1098 to reach 100 ng total DNA) mixed with 0.72 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... and 0.3 µL of TransIT-X2 (Mirus). Genomic DNA was extracted ∼72 hours post transfection using the DNAdvance Kit (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... and transfected with 2 μg HaloTag-DCAF and 0.1 μg NanoLuc-BRD4 plasmids using TransIT-LT1 transfection reagents (Mirus Bio). The cells were incubated overnight before replating in an opaque white 384-well plate at 2 x 105 cells/mL in FluoroBrite DMEM (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected using TransIT (Mirus Bio) and ∼292 fmol ACTB-4xenv8-FL-3’antiPNA (as described previously,13 Addgene plasmid #112058) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected using TransIT (Mirus Bio) and ∼448 fmol env8-AD-U1 as described previously,13 Addgene plasmid #112059 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected 24 hours after plating with 1.0 µg D2HGlo constructs and 3.0 µL Trans-IT®-LT1 transfection reagent (Mirus Bio, Madison, WI). The cells were allowed to express for 48 hours before fluorescence imaging.
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bioRxiv - Cell Biology 2024Quote: Constructs were forward transfected in HEK293T cells using transIT®-LT1 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: HEK293T were forward co-transfected with a plasmid encoding the protein of interest and a plasmid encoding the AFvariant of the Methanosarcina mazei pyrrolysine tRNA synthetase (pylRS-AF) using transIT®-LT1 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using transIT®-LT1 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected with the packaging vectors pCMV-dR8.91 and pMD2-G and the sgRNA library or dCas9-BFP-KRAB transfer vector using LT1 transfection reagent (Mirus MIR2300). Lentivirus-containing media was harvested after 48-72 hrs and filtered before use in downstream applications.
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bioRxiv - Cancer Biology 2024Quote: ... and 1 μg of the target shRNA plasmid using TransIT-LT1 transfection reagent (Mirus). After 48h ...