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Citations for Mirus Bio :
301 - 350 of 1473 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were cultured in a 100 mm plate overnight and plasmids (15 μg) were transfected using 45 μl of TransIT-LT1 (Mirus). The cells were cultured for 48 h ...
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bioRxiv - Cell Biology 2023Quote: ... using TransIT-LT1 transfection reagent according to the manufacturer’s instructions (Mirus 2304). Viral supernatants were collected for 3 days and filtered through a 0.45μm PES filter ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach 50%-70% confluency before transient transfection using TransIT-2020 (Mirus Bio, catalog #: MIR 5400) according to the manufacturer’s instruction.
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were grown in 15-cm dishes and allowed to reach ~70-80% confluency before transient transfection using TransIT-2020 (Mirus), according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with indicated experimental plasmid via TransIT-2020 (Mirus MIR5400) with a total of 0.5 µg DNA amount/condition/well ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... individual wells were transfected with plasmids encoding Venus fused BH3-proteins using TransIT-X2 reagent (Mirus). Non-transfected wells were treated with transfection reagent alone (no DNA added) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Cell Biology 2023Quote: ... The construct was transfected into low pass S2 cells using TransIT Insect transfection reagent (Mirus) and stable cells carrying indels for this locus were selected for with 5 μg/ ml puromycin for 3 weeks ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected with Cre-recombined ROSA-Kaleidoscope using TransIT-2020 (MIR5404, Mirus Bio). For western blots ...
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bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
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bioRxiv - Cancer Biology 2023Quote: ... Sanger sequencing-verified pX330 plasmids containing the correct sequences of gRNAs were transfected into KB1P-G3 cells using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or TransIT-LT1 (Mirus) reagents according to manufacturer instructions ...
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bioRxiv - Biophysics 2023Quote: ... The cells were transfected at a confluency of ∼80% with 600-1000 ng of plasmid and 6 uL of TransIT293T (Mirus) transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... Control and BCAT1-KO U251 cells were reverse-transfected with 1µg/ml of TC3-R9P-3NLS pDNA using 1:2 of Trans-iT (Mirus). 48h after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... DNAs were transfected into 293T cells using TransIT®-Lenti Transfection Reagent (Mirus, MIR2304). Viral supernatants were collected ...
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bioRxiv - Immunology 2023Quote: ... 1μg each of the eight plasmids were mixed well with 16 μL of TransIT-LT1 (Mirus Bio) and transfected into the hMDCK-HEK293 cocultured cells at 70% confluence ...
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bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 293T cells were transfected with a plasmid expressing SARS-CoV-2 Spike (kindly provided by Markus Hoffmann and Stefan Pöhlmann Hoffmann et al., 2020 PMID: 32142651) using TransIT-X2 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid transfections to generate stable cell lines were performed with TransIT®-LT1 transfection reagent (Mirus). 2×105 cells per well were seeded in 6-well plates the day before transfection ...
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bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection mixtures were prepared with 24 μl LT1 (Mirus-Bio), 800 μl Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... using TransIT-LT1 transfection reagent (Mirus Bio). The pseudoviruses were then harvested and concentrated at 48 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
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bioRxiv - Microbiology 2023Quote: ... along with 4.5 uL of TransIT-LT1 reagent (Mirus Bio LLC; MIR2305). VSVG pseudotyped lentivirus was harvested and filtered through a 0.22 um filter (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... or TransIT®-293 Transfection Reagent (MIR2700, Mirus Bio LLC, Madison, WI, USA). For production of VSV-G-pseudotyped HIV-1 ...
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bioRxiv - Immunology 2023Quote: ... All plasmid DNA transfections were conducted using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 60 µl transfection reagent TransIT-293 (Mirus Bio) was added and incubated for 30 min at RT and added to the 10 cm dish with HEK293T cells ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes of Transit-X2 (Mirus #MIR6000), Opti-MEM and siRNA were prepared in a concentration of 25nM and incubated at room temperature for 20 minutes before the siRNA complexes were added to the cells ...
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bioRxiv - Cell Biology 2023Quote: ... Ingenio® electroporation buffer (Mirus, Ref. MIR 50111), HiPerFect transfection reagent (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: HeLa cells inducibly expressing GFP and Flag-HA-tagged UNKWT were seeded in 6-well dishes and transfected 24 hours later at about 40% confluence using the TransIT-X2 Dynamic Delivery System (Mirus, MIR6003) with a pool of siRNAs targeting CNOT7 (Horizon ...
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bioRxiv - Molecular Biology 2023Quote: ... and 7.5ul TransIT-LT1 (Mirus) transfection reagent ...
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bioRxiv - Developmental Biology 2023Quote: ... we transfected S2 cells with pAC5.1-mcd8-CIBN-NTEV-NICD-GFP and pAC5.1-Cry2-mCherry-CTEV using the TransIT-Insect Reagent (Mirus, MIR #6104) according to manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected using TransIT-LT1 (Mirus Bio LLC, Madison, WI) with 0.75 μg third-generation lentiviral packaging mix (pMD2.G (Addgene #12259) ...
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bioRxiv - Genetics 2023Quote: ... Transfections of wild-type and Gly1170Ser COL2A1-encoding plasmids were performed using Transit-2020 transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using TransIT-293 transfection reagent (Mirus). Lentivirus was harvested 48 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were transfected using the TransIT-X2 reagent (Mirus #MIR6000). For transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... into HEK293T cells using TransIT-Lenti (Mirus Bio, Madison, WI). A 3:1 ratio of transfection reagent to total plasmid was used for all transfections ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This mix was combined with 9 μL of TransIT-LT1 (Mirus) and 15 µL of OptiMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivector were transfected into 10 million 293T using TransIT-LT1 reagent (Mirus). Supernatants were harvested at 48 and 72 hrs after transfection ...
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bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.4μg TOB1-AS1 overexpression vector and 22.8μl TransIT-LT1 Transfection Reagent (Mirus, MIR 2306) were mixed and incubated at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... spCas9 Nuclease V3 (IDT) and TransIT-X2® (Mirus Bio). Following initial transfection for 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected at 60-90% confluence with TransIT®-LT1 Transfection Reagent (#2300, Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Transfections were performed 20 hours following seeding of 2x104 HEK 293T cells per well in 96-well plates and contained 70 ng of ABE8e plasmid and 30 ng of sgRNA expression plasmid mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Co-transfection of siRNA was performed with TransIT-X2 (Mirus Bio) and experiments perfomed 48 h later.