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Citations for Mirus Bio :
101 - 150 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Vero E6 cells cultured in 24-well plates were transfected with a mixture of 25 nM siRNAs using the IT-X2 reagent (Mirus Bio LLC, USA). A second transfection was performed 24 h after the first transfection ...
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bioRxiv - Immunology 2024Quote: ... in Opti-MEM (cat. 31985070, Thermo-Fisher) with Trans-IT-293 transfection reagent (cat. MIR 2705, Mirus Bio) and cultured at 37°C for 12-15 hrs prior to changing the medium and culturing for an additional 24 hrs at 37°C ...
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bioRxiv - Microbiology 2024Quote: 1x 105 HeLa-GL cells/well were grown on coverslips with 500 µL medium in 24-well plates and one day later transfected with a control vector or expression vectors of SARS-CoV-2 E (Hu-1) or E T9I by using Opti-MEM reduced serum media and TransIT-LT1 Transfection Reagent (Mirus, #MIR2306) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Transient transfections of DNA were performed with TransIT-LT1 (Mirus Bio) for HEK293T cells ...
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bioRxiv - Immunology 2024Quote: ... Cells to be transfected were grown to 70-90% confluence and transfected with the indicated plasmid as per the Transit-LT1 (Mirus bio, MIR 2304) manufacturer recommendations with the exception that 2ug DNA and 6uL transfection reagent were used per well.
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bioRxiv - Microbiology 2024Quote: ... The resultant plasmids were introduced into HEK293T cell using TransIT293 (Mirus), and these KIRs were expressed as secreted human-Fc fusion proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... and with 1.5μg of plasmid expressing shRNA-Control or shRNA-Npm1 using Transit-X2 transfection reagent (Mirus) in Opti-MEM GlutaMAX reduced-serum medium (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection was done with TransIT-X2 transfection reagent (Mirus Bio, MIR6004) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with TransIT-293 (Mirus Bio) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 40lJng of prime editor plasmid and 13.3 ng of pegRNA PCR product (and 4.4 ng nicking sgRNA PCR product for PE3) using 0.6 µl of TransIT-X2 (Mirus) lipofection reagent for experiments in 96-well plates ...
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bioRxiv - Microbiology 2024Quote: ... Each well was transfected with 2.5 μg Spike-pCAGGS plasmid using TransIT®-LT1 Transfection Reagent (Mirus). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... and K562 cells were transfected with TransIT-2020 (Mirus Bio). HeLa cells were transfected with HeLa-Monster (Mirus Bio) ...
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bioRxiv - Neuroscience 2024Quote: sgRNA transfer plasmid with packaging (pCMVΔ8.91) and VSVG envelope (pMD.G) plasmids were transfected into HEK293T cells (Horizon Discovery) with TransIT-293 (Mirus). Media was collected on day 2 and 3 post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were transfected with HeLa-Monster (Mirus Bio). HDFa cells were transfected with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: Plasmid DNA was transfected into Huh7OK1 or 293T cells by using TransIT®-LT1 (Mirus Bio, Madison, WI). Lysate preparation and immunoprecipitation were carried out as described previously (21) ...
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bioRxiv - Microbiology 2024Quote: ... RNA (250ng/mL) was transfected using Transit-mRNA transfection kit (Mirus Bio) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... The cells were transfected using TransIT®-2020 transfection reagent (Mirus Bio™) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then counted and resuspended in D10 medium supplemented with 10 μg/mL of TransduceIT™ Transduction Reagent (Mirus Bio, Cat# MIR6620) and transferred to the wells pre-added with viral supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... cells were first transfected with either empty vector or HA-tagged full-length RelA overexpression plasmid using TransIT-Lenti (Mirus) following the manufacturers recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... and pRSV-Rev expression constructs into HEK293T using TransIT-Lenti transfection reagent (Mirus) for generation of lentiviral particles ...
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bioRxiv - Genomics 2024Quote: ... comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [59] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 333 ng of pMD2.G per well) using 3 μL of Trans-IT 293T transfection reagent (Mirus Bio) per μg of DNA ...
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bioRxiv - Genomics 2024Quote: ... pGYL1 contains a 318 nt mouse Alb promoter sequence derived from pLIVE plasmid (Mirus Inc., Madison, WI, #MIR 5420) [59] followed by a 1,653 nt firefly luciferase reporter sequence derived from plasmid pGL4.10 (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.2 µL of TransIT®-2020 transfection reagent (Mirus Bio) was diluted into 4 µL of Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 µg plasmid containing the appropriate transgene were used for transfection using TransIT-293 transfection reagent (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using TransIT-LT1 transfection reagent (Mirus). Retrovirus was harvested 48 hours post-transfection from cell culture supernatant.
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bioRxiv - Genetics 2024Quote: HEK293T cells were transfected with the TransIT®-LT1 transfection reagent (Mirus Bio) in 6-well plates with 1.25μg of plasmids for the CRISPR-construct and 1.25μg of plasmids for single gRNA ...
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bioRxiv - Immunology 2024Quote: ... using transIT-LT1 transfection reagent (Mirus, MIR2306). Lentivirus was harvested 48 and 72 hrs post-transfection by ultracentrifugation from cell culture supernatant (50,000 g at 4°C for 3 hours) ...
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bioRxiv - Microbiology 2024Quote: LMH cells were seeded into 6-well plates at a density of 8.0×10⁵ and transfected with 2.5 µg of GFP-tagged cIL-4 or GFP-tagged vIL-4 using TransIT (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells at 90% conluency were transfected using TransIT (Mirus) with 7.5 µg of plasmid containing the sgRNAs and cas9 gene according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus expressing specific constructs was produced by transfecting HEK-293 cells in 6-well plates with a 1:1:0.1 ratio of lentiviral vector:pMD2.G:psPAX2 using the TransIT-LT1 transfection reagent (Mirus). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... media was supplemented with 200 μg/mL hygromycin (Mirus Bio cat#MIR 5930) to initiate selection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were transiently transfected with 500 ng total DNA and 1.5 µL of TransIT-X2 (Mirus Bio, Madison, WI) in 100 µL Opti-MEM (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified mRNAs were then transfected to specific HEK cell lines as needed by Mirus TransIT®-mRNA Transfection Kit following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... into HEK293T cells using TransIT-Lenti (Mirus Bio, Madison, WI). A 3:1 ratio of transfection reagent to total plasmid was used for all transfections ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and 0.2 µg of pC-VSV-G (VSV-G envelope) using a 3:1 ratio of TransIT-293 (Mirus) transfection reagent to DNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and lentiviral transfer plasmid pLENTI-puro using TransIT-293 Transfection reagent (Mirus). Viral harvests were conducted at 24 and 48 hours post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 5ug dsRNA (TransIT-2020 reagent (Mirus)) and harvested or subjected to imaging 3 days later ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were transfected with pMTBS and pCS2-ST at ∼80% confluence using TransIT-293 transfection reagent (Mirus). Cells were harvested for downstream applications 48 hours post-transfection.
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bioRxiv - Genetics 2024Quote: ... using Trans-IT-293 transfection reagent (Mirus, MIR2700). Media was collected on day 2 after transfection and the lentivirus was concentrated using Lenti-X Concentrator (Takarabio ...
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bioRxiv - Microbiology 2024Quote: Huh7.NTCP cells seeded in a 48-well plates were transfected using TransIT-LT1 transfection reagent (Mirus) and lysed 72 hours later using the Bright-Glo Luciferase reagent (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... using TransIT-Lenti Transfection Reagent (Mirus Bio, Madison, WI). A 3:1 ratio of transfection reagent to total plasmid was used for all transfections ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.2 µg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 transfection reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Kc cells were transiently transfected with 400ng of each plasmid using the TransIT-2020 reagent (Mirus). Live imaging was performed 72 hours after transfection ...
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bioRxiv - Genetics 2024Quote: ... using the TransIT-LT1 Transfection Reagent (Mirus Bio MIR2304) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA to be delivered to each mouse by HDI was resuspended in TransIT-EE delivery solution (MIR 5340, Mirus, Inc, Madison, WI) in a final volume of ∼3 ml ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections of HEK293T cells was done using TransIT-LT1 (Mirus Bio; MIR 2304) per manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... and Tat using TransIT-LTI Transfection Reagent (Mirus). Viral supernatant was harvested 2 days after transfection and frozen at -80°C prior to transduction.
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bioRxiv - Cancer Biology 2024Quote: ... 3.4μg TOB1-AS1 overexpression vector and 22.8μl TransIT-LT1 Transfection Reagent (Mirus, MIR 2306) were mixed and incubated at room temperature for 30 minutes ...