Labshake search
Citations for PacBio :
401 - 450 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... (referred to as “1KGP” 9, 4,718 SVs detected using PacBio RSII by Peter et al ...
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bioRxiv - Genomics 2024Quote: ... and SMRTbell cleanup beads (Pacbio). We made a DNA repair step with the “SMRTbell Damage Repair Kit SPV3” (PacBio) ...
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bioRxiv - Plant Biology 2024Quote: ... Sixty grams of leaf tissue was collected and immediately flash frozen in liquid nitrogen before being shipped on dry ice to the Arizona Genomics Institute (AGI, University of Arizona) for high-molecular weight DNA extraction and PacBio Sequel II (PacBio, https://www.pacb.com) sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... Ten representative strains from each 95% ANI clade were selected for long-read Nanopore sequencing (Oxford) and compiled with public SRA data from previously sequenced strains (N=12 Illumina, N=3 PACBIO). Data were combined for each strain to perform a hybrid assembly with unicycler69 using a kmer count 31,41,51,61,71,81,91,95,101,105,111 and “mode” determined by ANI similarity to a reference strain (i.e. ...
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bioRxiv - Evolutionary Biology 2024Quote: Genome assembly from PacBio CLR reads was performed with FALCON-Unzip (Chin et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing primer annealing and polymerase binding to the PacBio SMRTbell Templates were performed according to the manufacturer’s recommendations (PacBio, US). The library complex was then sequenced using SMRT Cell 8M on sequel IIe system (PacBio).
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bioRxiv - Developmental Biology 2024Quote: ... The library complex was then sequenced using SMRT Cell 8M on sequel IIe system (PacBio).
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bioRxiv - Plant Biology 2024Quote: ... The sequencing chemistry used was Sequel® II Sequencing Plate 2.0 (PacBio®, P/N 101-820-200) and the Instrument Control Software v 10.1 ...
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bioRxiv - Plant Biology 2024Quote: ... The HiFi libraries were prepared according to the HiFi protocol version 03 (PacBio®, P/N 101-853-100) and the final libraries were size fractionated using the SageELF® system (Sage Science® ...
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bioRxiv - Plant Biology 2024Quote: ... Each sheared aliquot underwent AMPure® PB bead (PacBio®, P/N 100-265-900) purification and concentration before undergoing library preparation using the SMRTbell® Express Template Prep Kit 2.0 (PacBio® ...
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bioRxiv - Plant Biology 2024Quote: ... purification and concentration before undergoing library preparation using the SMRTbell® Express Template Prep Kit 2.0 (PacBio®, P/N 100-983-900). The HiFi libraries were prepared according to the HiFi protocol version 03 (PacBio® ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing internal control complex 1.0 (PacBio, 101-717-600) was spiked into the final complex preparation at a standard concentration before sequencing for all preparations ...
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bioRxiv - Plant Biology 2024Quote: ... The sequencing chemistry used was Sequel® II Sequencing Plate 2.0 (PacBio®, 101-820-200) and the Instrument Control Software v10.1.0.125432.
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting phylogenetic trees led to the manual removal of up to 16 sequences (6 OTUs from PR2 and 10 OTUs from the PacBio dataset) due to their long branches and dubious positions (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the SRE kit (Circulomics, SS-100-101-01, now PacBio) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Cancer Biology 2024Quote: ... or the SRE XS kit (Circulomics SS-100-121-01, now PacBio) when sample availability or concentration was limiting ...
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bioRxiv - Cancer Biology 2024Quote: Tumour genomic DNA was extracted from tissue with the Nanobind tissue kit (PacBio SKU 102-302- 100) and Germline DNA from blood with the Nanobind CBB kit (PacBio ...
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bioRxiv - Cancer Biology 2024Quote: ... and Germline DNA from blood with the Nanobind CBB kit (PacBio, SKU 102-301-900). After extraction ...
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bioRxiv - Genomics 2024Quote: ... using input data from PacBio HiFi long read reads ...
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bioRxiv - Genomics 2024Quote: ... Positions where either long-read technology (ONT and PacBio HiFi) confidently maps (MAPQ ≥ 10 ...
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bioRxiv - Genomics 2024Quote: High molecular weight DNA of three MSR2 and three susceptible individuals were submitted to whole genome sequencing by Pacific Biosciences (PacBio) Revio™ system with high fidelity (HiFi ...
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bioRxiv - Genetics 2024Quote: Four PacBio long read-RNA Iso-Seq samples (three samples prepared following the PacBio standard workflow, 1 sample prepared following the optimized PacBio long-transcript workflow) were analyzed with IsoQuant (Prjibelski et al. ...
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bioRxiv - Genetics 2024Quote: Four PacBio long read-RNA Iso-Seq samples (three samples prepared following the PacBio standard workflow ...
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bioRxiv - Genetics 2024Quote: SMRTbell libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences (PacBio)) ...
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bioRxiv - Genetics 2024Quote: ... The V2R mutagenesis libraries were multiplexed with other libraries and ran across two SMRT Cells on the Sequel IIe instrument (PacBio). The PacBio sequencing data was analyzed with alignparse75 ...
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bioRxiv - Genetics 2024Quote: ... and a PCR-free library was prepared using SMRTbell prep kit 3.0 (PacBio). Long-read whole-genome sequencing was done using a PacBio Revio system at an average coverage of approx ...
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bioRxiv - Genetics 2024Quote: ... and final libraries were eluted in 11–15 µl of Elution Buffer (PacBio).
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bioRxiv - Genetics 2024Quote: ... Sheared samples were further purified with 1X SMRTbell cleanup beads (PacBio), washed twice with 80% ethanol ...
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bioRxiv - Genetics 2024Quote: ... and eluted in 47 µl of Low TE buffer (PacBio). DNA concentration was again determined using the Qubit dsDNA HS assay (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... Sequencing primers and polymerase were bound to each library pool using the Sequel® II Binding Kit 3.2 (PacBio). Polymerase-bound SMRTbell complexes were purified ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting nuclei/cellular compartment mix was used as input for the Nanobind plant nuclei big DNA kit from PacBio. Steps were followed as listed in the kit protocol except for large cell inputs ...
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bioRxiv - Evolutionary Biology 2024Quote: ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: An initial draft hybrid assembly was constructed from raw Illumina short read data and PacBio longread data using the MaSuRCA pipeline (kmer size = 99, PacBio coverage = 30x). Contigs from this draft assembly were then arranged into scaffolds using the Hi-C and Chicago data using the Dovetail HiRise pipeline ...
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bioRxiv - Evolutionary Biology 2024Quote: ... High molecular weight DNA was extracted for Pacific Biosciences (PacBio) and Oxford Nanopore (ONT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sing1 gDNA was sequenced using the Pacific Biosciences (PacBio) single- molecule real-time sequencing (SMRT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolated DNA from this protocol was processed with the Short Read Eliminator kit from PacBio to deplete DNA fragments < 25kb in length ...
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bioRxiv - Developmental Biology 2024Quote: We published the RNA-seq library preparation and sequencing (PacBio project PRJNA659550). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... this individual was used for all aspects of genome assembly (PacBio, Hi-C, RNAseq). The remaining specimens from the same collecting event are retained in the University Putra Malaysia Marine Collection (UPMMC) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genome assemblies and gene annotations based on long-read sequencing (PacBio) of the Z ...
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bioRxiv - Genomics 2023Quote: ... Library construction for HIFI protocol was done using SMRTbell Express Template Prep Kit 2.0 (PacBio), followed by another round of AMPure PB Beads Purification and Bluepippin size selection (< 8kbp) ...
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bioRxiv - Genomics 2023Quote: ... Samples were sequenced with Sequel II Sequencing Kit 2.0 on PacBio Sequel II (PacBio).
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bioRxiv - Genomics 2023Quote: ... DNA samples were purified and concentrated using AMPure PB beads (PacBio, Menlo Park, USA) to remove small fragments and impurities prior to library preparation ...
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bioRxiv - Microbiology 2023Quote: High-quality HiFi reads were extracted from PacBio sequencing data requiring a minimum of 3 full passes in CCS command (v6.0.0 ...
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bioRxiv - Microbiology 2023Quote: ... The assemblies were produced using third generation long reads (PacBio Sequel / PacBio Hifi, BioNano®) and Hi-C ...
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bioRxiv - Microbiology 2023Quote: The assembly of the mitochondrial genome sequence was done with ALADIN (https://github.com/GDKO/aladin) using the mitochondrion mode from PacBio HiFi reads ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared with the Illumina Nextera DNA flex and Pacific Bioscience (PacBio) SMRTbell express template prep kit 2.0 ...
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bioRxiv - Microbiology 2023Quote: ... the sequencing library was prepared from PacBio high fidelity (HiFi ...
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bioRxiv - Genomics 2023Quote: ... input amounts of 500 ng provide sufficient library yield for approximately one full (non-multiplexed) Pacific Biosciences (PacBio) Sequel II instrument sequencing run ...
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bioRxiv - Genomics 2023Quote: De novo variant calling was done using deepvariant (Poplin et al. [2018]; v1.1.0; model_type=PACBIO) using the HIFI host reads aligned to GRCm39 with pbmm2 (v1.4.0 ...
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bioRxiv - Immunology 2023Quote: ... Tools from PacBio & Bioconda were used to process these data (https://github.com/PacificBiosciences/pbbioconda) ...