Labshake search
Citations for PacBio :
401 - 450 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and SMRTbell Enzyme Clean-up kit v1 (PacBio™), and prepared using the Sequel II Binding Kit 2.2 (PacBio™) ...
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bioRxiv - Genomics 2024Quote: ... Long read sequencing was performed using the SMRT Link software version 10.2.0.133434 on a Sequel IIe Pacific Biosciences (PacBio™) device using 6 x SMRT Cells following size selection (> 3 kb ...
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bioRxiv - Genomics 2024Quote: ... Libraries were cleaned using the Express TPK 2.0 (PacBio™) and SMRTbell Enzyme Clean-up kit v1 (PacBio™) ...
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bioRxiv - Genomics 2024Quote: ... and prepared using the Sequel II Binding Kit 2.2 (PacBio™), Sequencing Primer v5 (PacBio™ ...
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bioRxiv - Genomics 2024Quote: ... A-tailed and ligated with overhang non-barcoded adaptors using SMRTbell Express 2.0 kit (PacBio Catalog #100-938-900). PacBio Sequencing primer is then annealed to the SMRTbell template library and sequencing polymerase was bound to them using Sequel II Binding kit 2.0 (PacBio Catalog #101-789-500) ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... de novo assemblies were generated from Pacbio-HiFi data using HIFIASM v19.5.2 (Cheng et al ...
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bioRxiv - Genomics 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (Pacbio) ...
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bioRxiv - Genomics 2024Quote: ... We made a DNA repair step with the “SMRTbell Damage Repair Kit SPV3” (PacBio). DNA were purified then sheared using the Megaruptor1 system (Diagenode ...
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bioRxiv - Genomics 2024Quote: The same DNA samples as used for Tell-Seq were sequenced by PacBio HiFi at the Norwegian Sequencing Centre ...
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bioRxiv - Genomics 2024Quote: ... and SMRTbell cleanup beads (Pacbio). We made a DNA repair step with the “SMRTbell Damage Repair Kit SPV3” (PacBio) ...
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bioRxiv - Bioengineering 2024Quote: High molecular weight genomic DNA was isolated from NHP liver tissues using Nanobind Tissue kit from PacBio (Cat. No. 102-302-100) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: Circulomics Short Read Eliminator XS (PacBio, Menlo Park, CA, United States) was used to remove small fragments ...
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bioRxiv - Biochemistry 2024Quote: ... Ten representative strains from each 95% ANI clade were selected for long-read Nanopore sequencing (Oxford) and compiled with public SRA data from previously sequenced strains (N=12 Illumina, N=3 PACBIO). Data were combined for each strain to perform a hybrid assembly with unicycler69 using a kmer count 31,41,51,61,71,81,91,95,101,105,111 and “mode” determined by ANI similarity to a reference strain (i.e. ...
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bioRxiv - Cancer Biology 2024Quote: Tumour genomic DNA was extracted from tissue with the Nanobind tissue kit (PacBio SKU 102-302- 100) and Germline DNA from blood with the Nanobind CBB kit (PacBio ...
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bioRxiv - Cancer Biology 2024Quote: ... and Germline DNA from blood with the Nanobind CBB kit (PacBio, SKU 102-301-900). After extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the SRE kit (Circulomics, SS-100-101-01, now PacBio) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Cancer Biology 2024Quote: ... or the SRE XS kit (Circulomics SS-100-121-01, now PacBio) when sample availability or concentration was limiting ...
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bioRxiv - Developmental Biology 2024Quote: We published the RNA-seq library preparation and sequencing (PacBio project PRJNA659550). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... this individual was used for all aspects of genome assembly (PacBio, Hi-C, RNAseq). The remaining specimens from the same collecting event are retained in the University Putra Malaysia Marine Collection (UPMMC) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genome assemblies and gene annotations based on long-read sequencing (PacBio) of the Z ...
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bioRxiv - Genomics 2024Quote: High molecular weight DNA of three MSR2 and three susceptible individuals were submitted to whole genome sequencing by Pacific Biosciences (PacBio) Revio™ system with high fidelity (HiFi ...
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bioRxiv - Genetics 2024Quote: SMRTbell libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences (PacBio)) ...
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bioRxiv - Genetics 2024Quote: ... The V2R mutagenesis libraries were multiplexed with other libraries and ran across two SMRT Cells on the Sequel IIe instrument (PacBio). The PacBio sequencing data was analyzed with alignparse75 ...
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bioRxiv - Genetics 2024Quote: ... and a PCR-free library was prepared using SMRTbell prep kit 3.0 (PacBio). Long-read whole-genome sequencing was done using a PacBio Revio system at an average coverage of approx ...
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bioRxiv - Genetics 2024Quote: ... and final libraries were eluted in 11–15 µl of Elution Buffer (PacBio).
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bioRxiv - Genetics 2024Quote: ... Sheared samples were further purified with 1X SMRTbell cleanup beads (PacBio), washed twice with 80% ethanol ...
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bioRxiv - Genetics 2024Quote: ... and eluted in 47 µl of Low TE buffer (PacBio). DNA concentration was again determined using the Qubit dsDNA HS assay (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... Sequencing primers and polymerase were bound to each library pool using the Sequel® II Binding Kit 3.2 (PacBio). Polymerase-bound SMRTbell complexes were purified ...
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bioRxiv - Genetics 2024Quote: Four PacBio long read-RNA Iso-Seq samples (three samples prepared following the PacBio standard workflow, 1 sample prepared following the optimized PacBio long-transcript workflow) were analyzed with IsoQuant (Prjibelski et al. ...
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bioRxiv - Genetics 2024Quote: Four PacBio long read-RNA Iso-Seq samples (three samples prepared following the PacBio standard workflow ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting nuclei/cellular compartment mix was used as input for the Nanobind plant nuclei big DNA kit from PacBio. Steps were followed as listed in the kit protocol except for large cell inputs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sing1 gDNA was sequenced using the Pacific Biosciences (PacBio) single- molecule real-time sequencing (SMRT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolated DNA from this protocol was processed with the Short Read Eliminator kit from PacBio to deplete DNA fragments < 25kb in length ...
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bioRxiv - Evolutionary Biology 2024Quote: ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: An initial draft hybrid assembly was constructed from raw Illumina short read data and PacBio longread data using the MaSuRCA pipeline (kmer size = 99, PacBio coverage = 30x). Contigs from this draft assembly were then arranged into scaffolds using the Hi-C and Chicago data using the Dovetail HiRise pipeline ...
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bioRxiv - Evolutionary Biology 2024Quote: ... High molecular weight DNA was extracted for Pacific Biosciences (PacBio) and Oxford Nanopore (ONT ...
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bioRxiv - Genomics 2024Quote: ... Positions where either long-read technology (ONT and PacBio HiFi) confidently maps (MAPQ ≥ 10 ...
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bioRxiv - Genomics 2024Quote: ... using input data from PacBio HiFi long read reads ...
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bioRxiv - Molecular Biology 2023Quote: ... SMRTbell library preparation from microbial gDNA and whole genome sequencing using the Pacific Biosciences Sequel II platform (PacBio) were performed by the NCI CCR Sequencing facility ...
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bioRxiv - Molecular Biology 2023Quote: ... Using the variant-barcode association table that was generated by PacBio sequencing (see (Flynn et al ...
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bioRxiv - Microbiology 2023Quote: ... The assemblies were produced using third generation long reads (PacBio Sequel / PacBio Hifi, BioNano®) and Hi-C ...
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bioRxiv - Plant Biology 2023Quote: ... A final polish was applied using Medaka (https://github.com/nanoporetech/medaka) (v0.11.2, ONT data) and Racon (Vaser, Sovic et al. 2017) (PacBio data) aiming at filling gaps resulting from the Hi-C and genetic map scaffolding ...
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bioRxiv - Genomics 2023Quote: ... and sequenced on a PacBio Sequel IIe platform (PacBio, Menlo Park, CA, USA). GenomeScope 2.0 [23] and KMC v3.2.1 [24] were employed for genome profiling.
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bioRxiv - Genomics 2023Quote: IsoSeq transcripts fasta file was generated using SMRT Link 12.0 (PacBio) with default parameters ...
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bioRxiv - Microbiology 2023Quote: ... CCS reads were filtered for those with size 3100- 3394bp and high-quality reads (Phred>20) using Filter FASTQ and mapped to the LASV reference seq (MH358389) with Minimap2 (PacBio HiFi reads vs reference mapping (- k19 -w19 -U50,500 -g10k -A1 -B4 -O6,26 -E2,1 -s200)) ...
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bioRxiv - Genomics 2023Quote: ... and 60× (PacBio). The same sample was also sequenced using Illumina Hi-C (Dovetail Genomics ...
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bioRxiv - Microbiology 2023Quote: ... although the precise number of mutations per spike is determined only after lentiviral genomes have been integrated into cells and sequenced with long-read sequencing (see Long-read PacBio sequencing for variant-barcode linkage section below) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The two assemblies (hybrid and PacBio only) were then combined using QuickMerge v0.2 (Chakraborty et al ...
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bioRxiv - Immunology 2023Quote: ... PacBio SMRTbell amplicon libraries for PacBio Sequel were constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...