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Citations for PacBio :
251 - 300 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The Short Read eliminator kit from PacBio was also used to increase average fragment size.
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bioRxiv - Genomics 2024Quote: ... the DNA was used for CCS (circular consensus sequencing) library preparation using the SMRTbell Express Template Prep Kit 2.0 and Enzyme Cleanup Kit 1.0 (PacBio). Each library was barcoded using PacBio Barcoded Overhang Adapters ...
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bioRxiv - Genomics 2024Quote: ... and the pellet was processed immediately using the Nanobind CBB DNA extraction kit (102-301-900, PacBio). Cell counts ranged from 1.6 to 4.22 x 106 cells ...
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bioRxiv - Genomics 2024Quote: ... including unique barcoding of each sample with the SMRTbell Adapter Index Plate 96A (102-009-200, PacBio). Size selection was performed with AMPure PB beads (102-182-500 ...
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bioRxiv - Genomics 2024Quote: ... Size selection was performed with AMPure PB beads (102-182-500, PacBio) at a 2.9× ratio ...
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bioRxiv - Genomics 2024Quote: ... Clean-up and concentration of the sheared material were conducted using SMRTbell clean-up beads (102-158-300, PacBio), and the DNA was eluted in 47 µL ...
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bioRxiv - Genomics 2024Quote: SMRTbell libraries were prepared using the SMRTbell® Prep Kit 3.0 (102-141-700, PacBio) following standard procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ligation product was split into two reactions and enriched using 10-18 cycles of PCR using barcoded amplification oligos (IDT) and SMRTbell® gDNA Sample Amplification Kit (PacBio). Up to sixteen libraries were pooled in equimolar concentrations and the pooled libraries were size-selected using the 0.75% agarose gel cassettes with Marker S1 and High Pass protocol on the BluePippin (Sage Science) ...
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bioRxiv - Molecular Biology 2024Quote: ... end-repair/A-tailing mix and ligated with amplification adapters using SMRTbell Express Template Prep Kit 3.0 (PacBio) and purified with SMRTbell cleanup beads ...
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bioRxiv - Microbiology 2024Quote: ... pbcoretools from PacBio github (https://github.com/PacificBiosciences) tools were used to determine the methylome of HSJ1 WT from PacBio data sequencing based on Ba HSJ1 genome assembly (120 ...
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bioRxiv - Microbiology 2024Quote: ... pbcoretools from PacBio github (https://github.com/PacificBiosciences ...
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bioRxiv - Microbiology 2024Quote: ... Ba strains CEP0996 and HSJ1 and their respective variant’s genomes were sequenced using either Oxford Nanopore Technology (ONT, by MIGS) or Pacific Biosciences technology (PacBio, by Genome Quebec) RS II complemented by Illumina technology (MIGS).
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bioRxiv - Microbiology 2024Quote: ... Long DNA fragments were enriched using SRE-XL short read eliminator buffer (PacBio) prior to library preparation and nanopore sequencing ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared using SMRT-bell Express Template Prep kit 2.0 (PacBio, #100-938-900) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Full-length cDNAs were used to construct sequencing libraries with the SMRTbell Template Prep Kit 2.0 (PacBio Biosciences), and sequenced on the PacBio Sequel II platform using the Sequel Binding Kit 2.0 (PacBio Biosciences).
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bioRxiv - Genomics 2024Quote: ... The environment for tree exploration (ETE) toolkit was used to calculate the normalized Robinson-Foulds (RF) symmetric difference between each tree pair (parent PacBio ASVs and pseudo-MiSeq ASVs) [25].
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bioRxiv - Genomics 2024Quote: ... and the sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The obtained DNA libraries were fractionated with BluePippin (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... Sequencing Primer v5 (PacBio™) and Sequel II Sequencing Plate v2.0 (PacBio™) ...
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bioRxiv - Genomics 2024Quote: All subreads from PacBio sequencing for each breed were assembled using Falcon (v2018.03.12 ...
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bioRxiv - Genomics 2024Quote: RNA was processed using the Iso-Seq Express 2.0 workflow (PacBio™), targeting transcripts up to 2 kb ...
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bioRxiv - Genomics 2024Quote: ... and SMRTbell Enzyme Clean-up kit v1 (PacBio™), and prepared using the Sequel II Binding Kit 2.2 (PacBio™) ...
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bioRxiv - Genomics 2024Quote: ... Long read sequencing was performed using the SMRT Link software version 10.2.0.133434 on a Sequel IIe Pacific Biosciences (PacBio™) device using 6 x SMRT Cells following size selection (> 3 kb ...
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bioRxiv - Genomics 2024Quote: ... Libraries were cleaned using the Express TPK 2.0 (PacBio™) and SMRTbell Enzyme Clean-up kit v1 (PacBio™) ...
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bioRxiv - Genomics 2024Quote: ... and prepared using the Sequel II Binding Kit 2.2 (PacBio™), Sequencing Primer v5 (PacBio™ ...
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bioRxiv - Genomics 2024Quote: ... A-tailed and ligated with overhang non-barcoded adaptors using SMRTbell Express 2.0 kit (PacBio Catalog #100-938-900). PacBio Sequencing primer is then annealed to the SMRTbell template library and sequencing polymerase was bound to them using Sequel II Binding kit 2.0 (PacBio Catalog #101-789-500) ...
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bioRxiv - Genomics 2024Quote: ... PacBio Sequencing primer is then annealed to the SMRTbell template library and sequencing polymerase was bound to them using Sequel II Binding kit 2.0 (PacBio Catalog #101-789-500). The prepared SMRTbell template libraries are then sequenced on a PacBio Sequel IIe sequencer using SMRT Link 10.2 ...
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bioRxiv - Genomics 2024Quote: ... and Version 2.0 sequencing chemistry with 1×1800 sequencing movie run times (PacBio, Menlo Park, CA).
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bioRxiv - Genomics 2024Quote: ... for 2-10 kb size selection and liked sizes are pooled at the equimolar ratios in a designated Degree-of-Pool in the worksheet using PacBio Multiplexing Calculator (PacBio, Benlo Park, CA). The pooled samples are end-repaired ...
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bioRxiv - Genomics 2024Quote: ... The amplified cDNA (11-14 cycles) is purified using 1x AMPure PB beads (PacBio Catalog #100-265-900) for non-size selection or BluePippin (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... SMRTbell libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, CA). Two single-molecule real-time (SMRT ...
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bioRxiv - Immunology 2024Quote: ... Publicly available long-read data (PacBio and Nanopore) validated the assemblies of chicken ...
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bioRxiv - Genomics 2024Quote: ... The methylation files (motifs and base modifications) of the genome was also obtained from PacBio’s SMRTAnalysis MotifMaker software.
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bioRxiv - Microbiology 2024Quote: ... Degenerate motifs obtained from PacBio SMRT sequencing analysis were then searched on Geneious Prime® 2024.0.5 (https://www.geneious.com ...
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bioRxiv - Microbiology 2024Quote: DNA samples were sent to Azenta Life Sciences (Suzhou, China) for Pacific Biosciences (PacBio) Single Molecule ...
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bioRxiv - Microbiology 2024Quote: ... and long-read (PacBio) shotgun sequences ...
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bioRxiv - Microbiology 2024Quote: ... was extracted with the Circulomics kit (PacBio) according to the manufacturer protocol for gram-positive and gram-negative bacteria ...
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bioRxiv - Genomics 2024Quote: High molecular weight genomic DNA was extracted from the milt using the Nanobind CBB kit (PacBio) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The Short Read Eliminator Kit (PacBio) was used to reduce the number of small DNA fragments according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ERGA’s sequencing strategy includes Oxford Nanopore Technology (ONT) and/or Pacific Biosciences (PacBio) for long-read sequencing ...
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bioRxiv - Genomics 2024Quote: ... The final HiFi libraries were constructed using a SMRTbell Prep Kit v3.0 (PacBio), size selected to 10-25 kbp using a PippinHT size selector (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... and the SMRTbell Express Template Prep Kit 2.0 (PacBio Cat. #100-938-900) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: The HiFi SMRTbell library was constructed using the SMRTbell gDNA Sample Amplification Kit (Pacific Biosciences [PacBio], Menlo Park, CA; Cat. #101-980-000) and the SMRTbell Express Template Prep Kit 2.0 (PacBio Cat ...
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bioRxiv - Genomics 2024Quote: ... We selected 2-4 genomes from each of these five STs and performed long-read sequencing using the Pacific Biosciences (PacBio) platform ...
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bioRxiv - Genomics 2024Quote: Assembled contigs were generated from PacBio HiFi reads using Hifiasm software with default parameters ...
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bioRxiv - Immunology 2024Quote: ... barcodes and amino acid variants were linked from PacBio sequencing using alignparse61 ...
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bioRxiv - Genomics 2024Quote: ... HiFi reads generated from PacBio sequencers were assembled using Hifiasm v0.19.7 using the ‘-primary’ option [34] ...
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bioRxiv - Genomics 2024Quote: ... and sequenced on the PacBio Sequel II platform using the Sequel Binding Kit 2.0 (PacBio Biosciences).
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bioRxiv - Genomics 2024Quote: ... The fractionated DNA libraries were sequenced on a SMRT Cell 8M on the Sequel II system (PacBio). HiFi reads were constructed using the CCS pipeline (https://ccs.how ...
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bioRxiv - Immunology 2024Quote: ... The high-fidelity reads obtained from PacBio sequencing were converted into a FASTA file and submitted to HighV-QUEST from IMGT33 ...
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bioRxiv - Genomics 2024Quote: ... and Sequel II Sequencing Plate v2.0 (PacBio™). Sequencing was performed using SMRT link software (10.2.0.133434 ...