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Citations for PacBio :
601 - 650 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Base-level CpG methylation was called using jasmine (PacBio). The percent CpG methylation at each genomic position was identified from a pileup of reads using pb-CpG-tools (https://github.com/PacificBiosciences/pb-CpG-tools) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Running Clair3 on a model trained with R9.4 and Guppy6 reads (or alternatively with HiFi for the two PacBio sequenced individuals), we generated gVCF files for each individual and combined them into multi-sample VCF files using GATK4 (Van der Auwera and O’Connor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... two samples were used for Pacific Biosciences (PacBio) HiFi sequencing and 21 samples for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the chromosome-level assembly dataset (PacBio reads; samples 3D1, 3D7, 1E4 and 1A5). We first subset gene sets to unambiguous CNV calls (i.e. ...
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bioRxiv - Genomics 2023Quote: 1,984,661 Pacific Biosciences high fidelity (PacBio HiFi) reads containing a total of 29,667,167,600 bases and a mean length of 14,948 bp were used for the assembly ...
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bioRxiv - Genomics 2023Quote: Three HPG1 accessions (Exposito-Alonso et al. 2018) were sequenced with one SMRT Cell on the Sequel II platform (PacBio). Plant growth (Contreras-Garrido et al ...
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bioRxiv - Genomics 2023Quote: ... For 5µg of DNA an extra repair step with SMRTbell DAMAGE REPAIR KIT (PACBIO, 100-465-900) was performed before the one step DNA damage repair + END-repair + dA tail of double stranded DNA fragments and adapters ligation ...
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bioRxiv - Genomics 2023Quote: We produced whole de novo genome assemblies of Anso77 and Doublon by combining long-read sequencing: ONT for Anso77 and ONT combined with Pacific Biosciences (PacBio, Menlo Park, CA, USA) for Doublon ...
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bioRxiv - Genomics 2023Quote: ... The sheared DNA was concentrated by bead purification using PB Beads (PacBio). The sequencing library was constructed following manufacturers protocols using SMRTbell Express Template Prep kit 2.0 ...
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bioRxiv - Genomics 2023Quote: ... and sequenced using Pacific Biosciences (PacBio) High Fidelity (HiFi ...
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bioRxiv - Genomics 2023Quote: ... and then subjected to HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2023Quote: ... the resulting SMRTbell library was purified and concentrated with 0.45X Ampure PB beads (PacBio, Cat. #100-265-900) for size selection using the BluePippin system (Sage Science ...
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bioRxiv - Genetics 2023Quote: ... DNA was then cleaned and sheared to an average size of 19 kb before generating four HiFi libraries using SMRTbell template express kit 2.0 (PacBio). Final libraries were size-selected in the size range of 7-50kb and sequenced across 18 SMRT cells (see SI for details).
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bioRxiv - Developmental Biology 2023Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Samples were sequenced using PacBio Sequel II 8M SMRT cell (version 2.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Iso-Seq Express Oligo Kit (PacBio) and SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Genomics 2023Quote: ... and telomeric reads were identified directly from PacBio CCS reads as previously described 20.
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bioRxiv - Evolutionary Biology 2023Quote: ... were subsequently constructed for single-molecule real-time (SMRT) sequencing to generate long high-fidelity (HiFi) reads with the Pacific Biosciences (PacBio) Sequel II system at the West German Genome Sequencing Center of HHU Düsseldorf.
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared a PacBio HiFi library with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was sequenced on a PacBio Sequel II instrument on an 8M SMRT cell in CCS mode with a 30-hour movie time ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted fasta files from the PacBio raw data (SMRT Link XML) using the ccs and bam2fastq commands from smrtools v9.0.0.92188 (PacBio). We assembled genomes using canu 2.1.1 [58] with options genomeSize=430m ...
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bioRxiv - Evolutionary Biology 2023Quote: We utilized the Pacific Biosciences (PacBio) HiFi long-read sequencing technology [81] ...
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bioRxiv - Genomics 2023Quote: PacBio HiFi libraries were prepared following PN 101-853-100 version 03 (PacBio) protocol ...
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bioRxiv - Genomics 2023Quote: ... Using SMRTBell template Prep Kit 1.0 (PacBio), a DNA and END damage repair step was performed on 15µg of unshared sample ...
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bioRxiv - Genetics 2023Quote: ... or spleen tissue from all eight founder strains and the HSRA strain were processed with the Pacific Biosciences (PacBio) Nanobind tissue kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... These WGAs were generated mostly with long-read sequencing data (PacBio or ONT), however we also included three WGAs generated with short-read sequencing data for S ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... The size selected and purified >10 kbp fraction of libraries were used for sequencing on Sequel II (Pacbio).
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bioRxiv - Plant Biology 2023Quote: ... a fraction of short fragments was removed from 9 μg of total DNA using the Short-Read Eliminator Kit XL (PacBio, SKU 102-208-400), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Library preparation of the amplified cDNA products was then performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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bioRxiv - Plant Biology 2023Quote: ... the DNA was sheared using Diagenode Megaruptor system and concentrated with AMPure PB Beads (PacBio, CA, USA). The libraries were size selected on a BluePippin System ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA peaks on the electropherogram were considered high quality and processed further for sequencing by PacBio.
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bioRxiv - Cancer Biology 2023Quote: The SMRTbell Express Template Kit (PacBio, cat. no. 100-938-900) was used following manufacturer’s instructions for DNA damage repair ...
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bioRxiv - Microbiology 2023Quote: ... CCS reads were filtered for those with size 3100- 3394bp and high-quality reads (Phred>20) using Filter FASTQ and mapped to the LASV reference seq (MH358389) with Minimap2 (PacBio HiFi reads vs reference mapping (- k19 -w19 -U50,500 -g10k -A1 -B4 -O6,26 -E2,1 -s200)) ...
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bioRxiv - Genomics 2023Quote: ... and loaded onto PacBio Sequel II (PacBio) on 8M SMRT cells (SRR25687008).
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bioRxiv - Genomics 2023Quote: ... and sequencing (PacBio HiFi ...
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bioRxiv - Genomics 2023Quote: ... barcoded universal primers (barcoded universal F/R primers plate 96 v2, PacBio) were attached to the forward and reverse universal sequences in the amplicons from the first-round L-PCR ...
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bioRxiv - Genomics 2023Quote: ... size selection and removal of excess primers was conducted using AMPure PB Beads (PacBio).
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from frozen pellets using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... this dataset was generated by PacBio sequencing of ∼4500 bp of the ribosomal operon (18S ...
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bioRxiv - Microbiology 2023Quote: The BHN418 genome assembly was generated by combining long read sequencing (PacBio) and short read sequencing (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... (London, UK) using a combination of Pacific Biosciences (PacBio) RS II single-molecule real-time (SMRT ...
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bioRxiv - Molecular Biology 2023Quote: ... A ready-to-sequence SMRTBell Polymerase Complex was created using a Binding Kit 3.0 (PacBio) and the primer V4 ...
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bioRxiv - Microbiology 2023Quote: ... Pacific Biosciences (PacBio), and Oxford Nanopore Technology (ONT ...
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bioRxiv - Molecular Biology 2023Quote: ... subreads coming from PacBio SEQUEL SMRTcells were processed via the SMRTLink’s IsoSeq v3.0 pipeline with default parameters (https://github.com/PacificBiosciences/IsoSeq/blob/master/isoseq-clustering.md) ...
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bioRxiv - Microbiology 2023Quote: We combined ASVs from the three Askov datasets (PacBio, and Nanopore UMI/UMI_clonal). ASVs were inferred using Unoise3 with -minsize 2 for the Nanopore UMI data and -minsize 3 for the Nanopore UMI_clonal and PacBio CCS data ...
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bioRxiv - Genomics 2023Quote: ... The library with 0.5-6 kb cDNA fragments was sequenced with Iso-Seq mode on PacBio Sequel II system (PacBio, USA).
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bioRxiv - Genomics 2023Quote: We performed both long read sequencing (Nanopore and PacBio) as well as short read sequencing (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and prepared into a sequencing library using the SMRTbell 3.0 Kit (Pacbio). The library was sequenced on the Pacbio Sequel IIe and the sequences were processed using SMRTlink 12.0.0 and Sequencher 3.4.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Using the variant-barcode association table that was generated by PacBio sequencing17 ...
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bioRxiv - Bioengineering 2023Quote: ... Bead purification was performed using PB Beads (PacBio, Menlo Park, CA). A PacBio sequencing library was constructed following the manufacturer’s protocol using a SMRTbell Express Template Prep kit 2.0 and size selected on a BluePippin instrument (Sage Science ...
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bioRxiv - Evolutionary Biology 2023Quote: ... elegans female of each genotype (Ao, Io, oo), using the Nanobind® Tissue Big Extraction Kit (Cat. No. NB-900-701-01, Circulomics Inc. (PacBio), MD ...