Labshake search
Citations for PacBio :
651 - 700 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II ...
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bioRxiv - Microbiology 2023Quote: ... For those strains we sequenced the genome by PacBio as described for the MRB collection19 ...
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bioRxiv - Genomics 2023Quote: ... we relied on a combination of protein and transcript evidence constructed from PacBio Iso-Seq and bulk Illumina RNA-seq for our focal species as well as related species in the tribe Sileneae ...
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bioRxiv - Genomics 2023Quote: ... using the Nanobind CBB DNA extraction kit (PacBio), according to the manufacturer’s instructions with modifications ...
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bioRxiv - Genomics 2023Quote: ... using Ampure-XP beads (0.5/0.68 for Pacbio, 0.68/0.8 for Illumina).
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bioRxiv - Evolutionary Biology 2023Quote: ... and long read (PacBio) datasets were assembled with hybrid assembler OPERA-MS69 ...
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bioRxiv - Plant Biology 2023Quote: ... Short read elimination was performed using the Circulomics Short Read Eliminator kit (formerly SS-100-101-01, now SKU 102-208-300; PacBio, Menlo Park, California, USA). The size selected DNA was quantified using a Qubit 1.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... This observation confirmed the high quality of the primary assembly achieved from PacBio reads including a post-processing step using Arrow (part of the GenomicConsensus package from PacBio) ...
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bioRxiv - Genomics 2023Quote: ... This observation confirmed the high quality of the primary assembly achieved from PacBio reads including a post-processing step using Arrow (part of the GenomicConsensus package from PacBio). Polishing ...
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bioRxiv - Genomics 2023Quote: ... DNA was pooled and sequenced at the Australian Genome Research Facility (Brisbane, Queensland, Australia) using a SMRTbell® prep kit 3.0 (PacBio), and circular consensus sequencing (CCS ...
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bioRxiv - Genomics 2023Quote: ... and sequenced with SMRT Cell 8 M on the Sequel II system (PacBio). The obtained HiFi reads were assembled with Hifiasm (Cheng et al ...
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bioRxiv - Immunology 2023Quote: ... the detected barcode sequences of both the antibody-screened and reference library were aligned to the barcode-variant dictionary generated using dms_variants (v0.8.9) from PacBio sequencing data of the BA.5 DMS library ...
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bioRxiv - Genomics 2023Quote: ... any amount of long-read sequences (e.g., PacBio) were used ...
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bioRxiv - Genomics 2023Quote: ... size selection using AMPure PB Beads (PacBio) was used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... based on long-read (PacBio) and short-read (Illumina ...
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bioRxiv - Neuroscience 2023Quote: Raw reads were processed and de-multiplexed with Iso-Seq (v3), aligned to the mouse reference genome (mm10, GENCODE) using pbmm246 (v1.10.0, a Minimap2 wrapper adapted for PacBio data ...
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bioRxiv - Genomics 2023Quote: We used the hifiasm v.0.15.2-r334 (Cheng, et al. 2021) assembler to generate contigs from PacBio HiFi sequencing data ...
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bioRxiv - Evolutionary Biology 2023Quote: A subsample of 10 individuals was selected for a complementary R gene analysis using Pacific Biosciences (PacBio) long-read sequencing ...
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bioRxiv - Microbiology 2023Quote: ... although the precise number of mutations per spike is determined only after lentiviral genomes have been integrated into cells and sequenced with long-read sequencing (see Long-read PacBio sequencing for variant-barcode linkage section below) ...
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bioRxiv - Genomics 2023Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II) ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... a custom origin set to position 7222 (between two tRNA molecules, tRNA-s1 and tRNA-s2, lacking both gene predictions and read coverage from the PacBio RNA sequence dataset) was used.
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bioRxiv - Genomics 2023Quote: ... The equal mass pool of eight samples was processed for sequencing using the standard IsoSeq protocol (PacBio protocol: PN 101-763-800 v2). The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England BioLabs Inc ...
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bioRxiv - Genomics 2023Quote: PacBio SMRT-Seq subreads for flow sorted MAC and MIC DNA were indexed with pbindex (PacBio SMRT Link v12.0.0). Falcon assemblies were indexed with samtools faidx.
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bioRxiv - Genomics 2023Quote: Purification steps were performed using 0.45X AMPure PB beads (PacBio). Thirty µg of DNA was purified to perform 3 libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... we searched the barcodes from PacBio data against the cell barcodes detected in the same sample in the 10x Illumina sequencing data to calculate the expression (UMI counts ...
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bioRxiv - Bioengineering 2023Quote: ... 2 weeks later and subjected to large deletions detection (PCR and PacBio sequencing). Single cells were isolated from bulk edited cells into 96-well plates 2 weeks after electroporation and genotyped 2 weeks after isolation ...
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bioRxiv - Genomics 2023Quote: ... Iso-Seq template preparation using the Iso-Seq Express kit followed by PacBio SMRT Cell sequencing on a PacBio Sequel II platform was performed by the Australian Genome Research Facility ...
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bioRxiv - Genomics 2023Quote: ... and 60× (PacBio). The same sample was also sequenced using Illumina Hi-C (Dovetail Genomics ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on the PacBio Sequel II (PacBio, CA, USA) at the Functional Genomics Center (FGCZ ...
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bioRxiv - Genomics 2023Quote: ... A Covaris G-Tube was then used to shear 10 µg of DNA to a size range of ∼10-30 kb followed by bead purification with PB Beads (PacBio). The HiFi sequencing library was constructed following manufacturer’s protocols using SMRTbell Express Template Prep Kit 2.0 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 following the manufacturer’s protocols (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900). HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat ...
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bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
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bioRxiv - Genomics 2023Quote: ... HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat. #100-265-900) for the removal of single-strand overhangs at 37°C for 15 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cortezi generated by PacBio HiFi technology (see above) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). PacBio HiFi whole-genome sequencing in circular consensus sequencing (ccs ...
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bioRxiv - Genomics 2023Quote: ... and two libraries with 15-20 kb inserts were prepared and sequenced on a PacBio Sequel II platform using Circular consensus sequence (CCS) mode (PacBio, USA). For Hi-C sequencing ...
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bioRxiv - Genomics 2023Quote: ... The transcript hints were obtained by mapping RNA sequences from PacBio Iso-Seq sequencing to the genome using GMAP v2020-10-27 (Wu and Watanabe ...
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bioRxiv - Molecular Biology 2023Quote: ... Then libraries were prepared (100-938-900, SMRTbell® express template prep kit 2.0, PacBio, CA, USA) and sequenced on the PacBio Sequel II (PacBio ...
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bioRxiv - Immunology 2023Quote: ... and the correspondence of variant and N26 barcode was obtained by PacBio sequencing on Sequel ll platform in Peking University throughput sequencing center (HTSC) ...
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bioRxiv - Molecular Biology 2023Quote: ... SMRTbell library preparation from microbial gDNA and whole genome sequencing using the Pacific Biosciences Sequel II platform (PacBio) were performed by the NCI CCR Sequencing facility ...
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bioRxiv - Molecular Biology 2023Quote: ... Using the variant-barcode association table that was generated by PacBio sequencing (see (Flynn et al ...
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bioRxiv - Microbiology 2023Quote: ... The assemblies were produced using third generation long reads (PacBio Sequel / PacBio Hifi, BioNano®) and Hi-C ...
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bioRxiv - Plant Biology 2023Quote: ... A final polish was applied using Medaka (https://github.com/nanoporetech/medaka) (v0.11.2, ONT data) and Racon (Vaser, Sovic et al. 2017) (PacBio data) aiming at filling gaps resulting from the Hi-C and genetic map scaffolding ...
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bioRxiv - Cell Biology 2023Quote: Genomic data generated in this study (RNA-seq, PacBio-seq and UMI4C-seq) was submitted to GEO with the Accession GSE160390.
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bioRxiv - Genomics 2023Quote: ... cerasus ‘Montmorency’ only) or flash-frozen in liquid nitrogen and stored at −80°C until extraction (for both PacBio SMRT sequencing and Illumina HiSeq) from a clone of ‘Montmorency’ and an accession of P ...
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bioRxiv - Genetics 2023Quote: ... which preserves 3’ and 5’ end information (PacBio, Menlo Park, CA). A PacBio SMRTbell library was constructed using these PCR-amplified full-length cDNA transcripts and sequenced using a Sequel II ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and their genomes were sequenced by PacBio HiFi mode according to the instructions from the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: The Cas9-mediated knock-in injection was performed by modifying previously described protocols for CRISPR/Cas9-mediated mutagenesis and used 0,75μg/μl nls-Cas9 protein (PacBio), 75ng/μl of each guide RNA ...
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bioRxiv - Genomics 2023Quote: ... both GATK and DeepVariant indel calls when using Illumina germline sequencing data, and only DeepVariant indel calls when using PacBio germline sequencing data). For GATK indel calls ...