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Citations for Promega :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: pSBtet-BP plasmids encoding various AAK1 WT and AAK1 mutant constructs alongside pCMV(CAT)T7-SB100 were co-transfected into ARPE-19 cells using FuGene HD transfection reagent (Promega), as per manufacturer’s protocol (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... and also further supplemented with CellTox CellTox Green Cytotoxicity Assay reagent (Promega G8741) by adding 1µL of the dye in 10mL of media ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase-Glo® 3/7 Assay was then performed according to the manufacturers’ protocol (Promega, Madison, Wi, USA) and 20 µM Ac-DEVD-CHO was added to select wells for each condition as a negative control ...
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bioRxiv - Cell Biology 2024Quote: ... The CellTiter-Blue® Cell Viability Assay was then performed according to the manufacturers’ protocol (Promega, Madison, Wi, USA). Plates were read using SpectraMax™ i3/i3x multi-mode plate reader.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then reverse transcribed to cDNA using the AMV reverse transcriptase (Promega, M510F). qPCR experiments were performed to assess the levels of cDNA using primers referenced in Extended Data Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... 15 µL of lysis solution (Promega G182A) was added 1 h prior to XTT working solution ...
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bioRxiv - Genomics 2024Quote: ... Fugene HD (Promega) was used for transfection at a 3:1 reagent:DNA ratio ...
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bioRxiv - Genetics 2024Quote: ... 500–800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR using GoTaq green master mix (Promega M7122).
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bioRxiv - Molecular Biology 2024Quote: ... One microgram sequence-grade modified trypsin (#V5111, Promega) was then added for on-bead trypsin digestion at 37°C for 16 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included goat anti-rabbit IgG HRP-conjugated antibody (1:5,000, #7074S, CST) and goat/rabbit/donkey anti-mouse IgG HRP Conjugate (1:5,000, #W402B, Promega). The membranes for streptavidin blotting were blocked with 3% BSA ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 before adding 4 µL of 0.5 µg/µL sequencing grade trypsin (Promega) and incubated over night at 37°C ...
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bioRxiv - Microbiology 2024Quote: Amplification was set up with the qPCR kit (Promega, Madison, WI) and carried out on an ABI7500 with an initial melting temperature of 95°C for 2 mins and 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% ProteaseMAX (Promega) and 5% acetonitrile (ACN ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 40 U/mL rRNasin (Promega), 1% sodium deoxycholate ...
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bioRxiv - Molecular Biology 2024Quote: ... Luciferase activity was detected by a Dual-Luciferase assay kit (Beyotime) with Glomax 96 microplate luminometer (Promega) in luminometer mode ...
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bioRxiv - Genomics 2024Quote: ... gRNAs were transfected into cell using Fugene HD (Promega) at a manufacturer suggested 3:1 reagent:DNA ratio ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability and proliferation was assayed using CellTiter 96 Aqueous One Solution Reagent (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... followed by the determination of viable cells using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). The experiment was carried out in four biological replicates ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation in vitro was assessed using CellTiter (Promega) per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... and reverse-transcribed with AMV-RT (Promega) and random 9-mer primers ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were labelled at least 30 minutes prior to imaging with Halo ligand Janelia Fluor 646 (Promega) at a 1:1,000 dilution of 20 μM stock in DMSO and 10 uM Hoesct dye ...
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bioRxiv - Cancer Biology 2024Quote: ... and MTT-based cell viability reagent (CellTiter96, Promega, Madison, WI) was added at 48 h and allowed to incubate for ∼2 h before reading ...
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bioRxiv - Biochemistry 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... 840 ng trypsin (Promega) in 30 μL ABC was added to each tube ...
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bioRxiv - Biochemistry 2024Quote: ... For digestion with chymotrypsin (Promega), the same process was followed but digestion was allowed to proceed at 25°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-mouse IgG HRP conjugate (W4021; Promega, Madison, WI, USA) as secondary antibodies for 1.5 to 2.0 h at room temperature with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected in a 6-well plate with 1 μg ADGRG6 DNA and 3 μL transfection reagent Fugene 6 (Promega, PRE2693) at 60-70% confluency and incubated for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... followed by the addition of Nano-Glo® Substrate (Promega) (5 µL per well ...
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bioRxiv - Biochemistry 2024Quote: ... Firefly and Renilla luciferase activities were measured using a luminometer (Promega GloMax(R) Navigator with Dual Injectors) ...
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bioRxiv - Biochemistry 2024Quote: Cell viability was measured using CellTiter-Glo (Promega)’s protocol ...
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bioRxiv - Biochemistry 2024Quote: pcDNA5/FRT/TO-SFB-HELB (WT, D506G, and 3xA) plasmids (1 µg) were transfected into 1.0 × 105 cells using FuGENE HD (Promega) according to the manufacturer’s protocol followed by incubation at 37°C with 5% CO2 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega) was used as secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: The amount of intracellular Glu was measured in 1×106 cells using the kit Glutamate-Glo (J7021; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... followed by an overnight digestion with Trypsin (Promega, Madison, Wisconsin, USA) at a 1:100 ratio at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... followed by overnight digestion with Trypsin (Promega, Madison, Wisconsin, USA) at a 1:100 ratio at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were performed with the transfection reagent FuGENE® HD (Promega, Ref ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were performed with the transfection reagent FuGENE® HD (Promega, Ref: E2311, Promega GmbH, Walldorf, Germany) at a 2:1 FuGENE® HD:DNA ratio according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and 75 µL of Bright-Glo™ lysis buffer (ProMega) was added ...
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bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
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bioRxiv - Biochemistry 2024Quote: ... the luciferase activity was detected using OneGlo (Promega, Cat# E6130) and luminescence was recorded by an EnVision Plate reader using EnVision Manager v1.14 (Perkin Elmer) ...
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bioRxiv - Biochemistry 2024Quote: ... The luciferase activity was measured with ONE-Glo Luciferase reagent (Promega) with an Analyst HT plate reader ...
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bioRxiv - Biochemistry 2024Quote: The assay was performed as described previously.38 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table S5 ...
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bioRxiv - Biochemistry 2024Quote: Each sample was rehydrated at 4 °C for 30 min by adding the same volume of 10 ng/µL trypsin in 50 mM ammonium bicarbonate containing 0.01% ProteaseMAX Surfactant (Promega). During rehydration ...
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bioRxiv - Biophysics 2024Quote: CaMKIIα activity was assessed using the ADP-Glo Kinase Assay kit (#V9101, Promega) with CaMKIIα holoenzyme (#PR4586C ...
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bioRxiv - Biophysics 2024Quote: ... gel plugs were crushed into small pieces and 5–10 μg of sequencing-grade trypsin (Promega) per ∼100 μg protein were added ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...