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Citations for Promega :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... amplified fragments were subcloned into the pGEM-T-Easy Vector (Promega), and 6 clones were sequenced ...
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bioRxiv - Plant Biology 2024Quote: ... for CDS of the FLC gene cloned into the pGEM®-T Easy vector (Promega, Madison, Wisconsin, US). Oligonucleotides and construct IDs are listed in Table S4.
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bioRxiv - Plant Biology 2024Quote: ... using Maxwell 16 LEV Plant RNA kit (Promega, USA). For each genotype/treatment combination ...
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... on a GM-2000 luminometer (Promega).
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bioRxiv - Pathology 2024Quote: ... at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega, VA1170) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2024Quote: ... and digested by trypsin (Promega V5280) at 1:100 w:w enzyme-to-sample ratio for each sample in 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2024Quote: ... the LUC and REN values were measured 24 hours later using the Dual-Luciferase® 80 Reporter Assay System (E1910, Promega, WI, USA)34 ...
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bioRxiv - Plant Biology 2024Quote: ... Rosette leaf samples were ground in liquid nitrogen and total RNA was extracted using the SV total RNA isolation system (Promega, www.promega.com). After quantification with a Nanodrop system (www.nanodrop.com) ...
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bioRxiv - Plant Biology 2024Quote: ... and DNase I was added to digest the genomic DNA (Promega, no. Z3100) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The blot was washed as before and then visualised with Attophos Substrate Kit (Promega, USA) using the Bio-Rad ChemiDoc XRS+ system.
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bioRxiv - Synthetic Biology 2024Quote: ... 1:50 (v/v) dilution of Nano-Glo substrate (Promega), and 10 ng/μL of DNA template from encoding the HiBit peptide64 (final volume of 8 μL) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Plant Biology 2024Quote: ... Amplicon concentration was quantified with a ND 1000 UV–vis spectrophotometer (NanoDrop Technologies) and Quantus (Promega, USA).
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Plant Biology 2024Quote: ... HaloTag beads (Promega) were aliquoted with 2x buffer volume ...
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bioRxiv - Plant Biology 2024Quote: Total plant RNA was treated with DNase I (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... mRNAs were translated in wheatgerm extract (Promega) supplemented with 20 mM amino acids (lacking methionine ...
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bioRxiv - Plant Biology 2024Quote: ... RNAsin (Promega) was added to the cleared lysate at 1.7 U/μL ...
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bioRxiv - Plant Biology 2024Quote: ... The second PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega) and the Agencourt AMPure XP Kit (Beckman Coulter) ...
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bioRxiv - Physiology 2024Quote: ... The optical density of the standards and samples was recorded at 450 nm wavelength using a microplate reader (Promega, GloMax 9301). All samples were run in triplicates.
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bioRxiv - Cancer Biology 2024Quote: ... where cell viability/ATP production was determined using CellTiter-Glo (Promega).
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Physiology 2024Quote: ... or anti-mouse HRP (Promega) was diluted in TBST containing 5% dried nonfat milk ...
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bioRxiv - Physiology 2024Quote: ... anti-rabbit HRP (Promega) or anti-mouse HRP (Promega ...
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bioRxiv - Physiology 2024Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: RNA from gonadal white adipose tissue was extracted with the SV Total RNA Isolation System (Promega, Madison, WI). RNA concentration was measured by a NanoDrop (Thermo-Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... Enzymatic digestion was performed using Trypsin (Promega, V528X) at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, US) as previously described (56) ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...
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bioRxiv - Plant Biology 2024Quote: Each 20 µl endpoint PCR reaction contained 10 µl of Gotaq Green Master Mix (2X) (Promega, Madison, WI), 1 µl (5 µM ...
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bioRxiv - Plant Biology 2024Quote: ... Dual luciferase assays (Promega) with four independent replicates were employed as reported previously (Schmidt et al. ...
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bioRxiv - Immunology 2024Quote: Cell death was analyzed by using a CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: NAD+/NADH ratio measurements were made using the NAD/NADH-Glo assay (Promega, G9071) according to the manufacturer’s instructions with modifications for measuring NAD+ and NADH individually (rather than pooled) ...
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bioRxiv - Biochemistry 2024Quote: ... trypsin (Promega, sequencing grade) was added at a 1:10 (trypsin ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Genomics 2024Quote: ... Individual DNAs were quantified using the QuantiFluor® dsDNA System kit (Promega), adjusted and equimolarly mixed to yield a female and a male pool ...
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bioRxiv - Genomics 2024Quote: ... (viii) checking libraries on the Bioanalyser for expected insert size and concentration (QuantiFluor® dsDNA System kit, Promega), (ix ...
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bioRxiv - Genomics 2024Quote: ... the activity of firefly luciferase was analyzed using the Dual-Glo® Luciferase Assay System (Promega, E2920), along with the Renilla luciferase control ...
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bioRxiv - Genomics 2024Quote: ... we constructed reporter plasmids based on pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega). We used ClonExpress II One Step Cloning Kit (Vazyme ...
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bioRxiv - Immunology 2024Quote: The PCR products representing genomic and cDNA sequences were purified using PCR purification kit (Promega) and subsequently sequenced using Sanger sequencing at Micromon Genomics ...
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bioRxiv - Biochemistry 2024Quote: ... Halo-SIN3A was cloned from ORF Clone # FHC11647 (Promega) by first mutating the codon for amino acid 109 from GCT (A ...
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bioRxiv - Genetics 2024Quote: ... pRLSV40 (Promega), expressing Renilla luciferase ...
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bioRxiv - Biophysics 2024Quote: ... FuGENE® HD transfection reagent was purchased from Promega (Madison, WI). CF®568 succinimidyl ester (CF568-NHS ...
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bioRxiv - Genetics 2024Quote: ... Purified PCR products (deletion-bearing, second-step amplicon) and the pGL4.23 vector (Promega) were double digested with NheI-HF (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... using FuGENE HD Transfection Reagent (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... were cloned upstream of a minimal promoter driven firefly luciferase reporter gene in the pGL4.23 vector (Promega). Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions ...
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bioRxiv - Biophysics 2024Quote: ... 800 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...