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Citations for Promega :
151 - 200 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... UA buffer was exchanged with 50 mM ammonium bicarbonate and sequencing-grade trypsin (Promega, Madison, WI, USA) was added at an enzyme to protein ratio of 1:50 ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Systems Biology 2024Quote: ... we incorporated polyethylene glycol (PEG 8000; Promega) at a concentration of 10% w/v in each reaction ...
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bioRxiv - Systems Biology 2024Quote: ... 2.5 μL of DNase (1 U/μL, T7 RiboMAX; Promega) was added to the reaction and further incubated for 30 minutes at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... Residual DNA was removed using the RQI RNAse-Free DNase kit (Promega). Total RNA was then quantified using the Agilent RNA 6000 nano kit (catalogue number ...
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bioRxiv - Systems Biology 2024Quote: ... 0.001% Digitonin (Promega)) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Systems Biology 2024Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... in live cell substrate (LCS) dilution buffer (Nano-Glo® assay, Promega) was added to each well ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 μL of 20-fold diluted furimazine stock (Promega) in live cell substrate (LCS ...
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bioRxiv - Systems Biology 2024Quote: ... 25μL of CellTiter-Glo (Promega Corp, Madison, WI) were added to each well ...
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and luminescence was quantified using a GloMax Explorer microplate reader (Promega). Percentage cytotoxicity was determined as ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91 (1.33μg) using 10ul of Fugene HD (Promega #E2312), according to manufacturer procedure ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were transiently transfected using Fugene HD (Promega #E2311), according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miRNA plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... After 48 hours each plate was treated with 25 mL’s of CellTiter Glo (Promega Corp G7572) and read in enhanced luminescence mode on an EnVision Multi-Label plate reader (Revvity Corp.) ...
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bioRxiv - Systems Biology 2024Quote: ... the Saccharomyces cerevisiae tryptic digest was purchased from Promega (Cat No. V7461) and analyzed as described in Pabst et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... a stable Firefly Luciferase (Luc2, Promega) has been used in order to compare it with the original biosensor.
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bioRxiv - Microbiology 2024Quote: ... the genomic DNA of the mutants was isolated using a Wizard Genomic DNA Purification Kit (Promega). The isolated genomic DNA was analyzed by agarose electrophoresis and quantified using a NanoDrop spectrophotometer ...
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2024Quote: ... and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega). The substrate was prepared according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the Renilla Luciferase Assay System (Promega). The substrate was prepared according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then 30 µl of premixed Nano-Glo substrate/buffer (Promega #N1138 and Promega #N1128 prepared following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... was performed with the Nano-Glo® HiBiT Lytic Detection System (Promega). Precultures and cultures for the measurements were grown in 96-well plates in the Tecan infinite f200pro microplate reader as described above ...
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the ONE-Glo Luciferase Assay System (Promega) and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then 30 µl of premixed Nano-Glo substrate/buffer (Promega #N1138 and Promega #N1128 prepared following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated amplicons were cloned in pGEM-T-Easy (Promega, USA) and sequenced (Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... amoA gene amplicons were generated using 0.6 ng DNA template with GoTaq DNA polymerase (Promega) and 0.5 μM primers (CamoA_19F [5’-ATGGTCTGGYTWAGACG-3’] and TamoA_632R-4 [5’-GCKGCCATCCATCKRTANGTCCA-3’] ...
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bioRxiv - Synthetic Biology 2024Quote: Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with a modified pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). Primary T cells were thawed the same day ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using oligo(dT) primers and M-MLV Reverse Transcriptase (Promega, Madison, WI) at 42°C for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation, Madison, WI, USA), without fragment separation ...
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bioRxiv - Immunology 2024Quote: ... and development using NBT/BCIP substrate mix (Promega) diluted in AP buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... a Firefly Luciferase with two destabilization sequences has been used (Luc2CP, Promega), while for the biosensing experiments ...
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bioRxiv - Synthetic Biology 2024Quote: ... Firefly Luciferase Arbitrary Units (Luciferase [A.U.]) were calculated by normalizing each sample’s Firefly Luciferase activity to the constitutive Renilla (pRL-TK, Promega) activity detected in the same sample ...
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bioRxiv - Synthetic Biology 2024Quote: Firefly Luciferase and Renilla Luciferase expression were measured following the protocol of Dual Luciferase Assay (Promega) on a Glomax Explorer plate reader (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... on a Glomax Explorer plate reader (Promega). The cells were collected 48 hours after transfection and lysed with 5X Passive Lysis Buffer (Biotin ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg of sequencing-grade trypsin (Promega) and 125 μL of digestion buffer (50 mM TEAB ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse IgG (Promega, 1:10,000), goat anti-rabbit IgG (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... and splice acceptor (SA)) were derived from the chimeric intron included in the pCI mammalian expression vector (Promega). We used a P2A sequence to prematurely truncate the SEED-target and facilitate expression of a transgene payload ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rabbit IgG (Promega, 1:20,000), goat anti-rat IgG + IgM (L+M ...
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bioRxiv - Synthetic Biology 2024Quote: ... All amplicons were then verified by gel electrophoresis and purified using a Wizard Gel Purification kit (Promega, Madison, WI).
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bioRxiv - Biochemistry 2024Quote: ... or PKA (Promega) kinases in phosphorylation buffer ...
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bioRxiv - Biochemistry 2024Quote: ... CK1 (Promega), or PKA (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Genetics 2024Quote: ... 2.5mM dNTP mix(Promega), ERCC RNA Spike-In Mix (1:3.2×107 Thermo Fischer scientific 4456740)) ...
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bioRxiv - Genetics 2024Quote: ... The sgRNA templates were transcribed using T7 polymerase with RiboMAX™ Large-Scale RNA Production Systems (Promega, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using Bright-Glo luciferase substrate (Promega) per the manufacturer’s recommendations ...