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Citations for Promega :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... and LUC activity was quantified with the Promega GloMax Navigator microplate luminometer (Promega, USA).
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bioRxiv - Plant Biology 2024Quote: ... and DNAse treated using RQ1 (Promega, USA). Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated using the Maxwell 16 LEV simply RNA tissue kit (Promega, #AS1280), and RNA integrity was assessed using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCRs were conducted using GoTaq DNA Polymerase (Promega) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2024Quote: ... 10μl Recombinant RNasein© Ribonuclease inhibitor (N2515; Promega) was added to inhibit RNaseA activity and samples were flash frozen in liquid nitrogen until analyzed ...
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bioRxiv - Neuroscience 2024Quote: ... Prior to overnight digestion at 37 °C using modified porcine trypsin (Mass Spectrometry Grade, Promega, Madison, WI, USA) at an enzyme–protein ratio of 1:80 ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Developmental Biology 2024Quote: ... Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega) in a Synergy H1 Hybrid Multi-Mode Microplate Reader (Thermo).
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... The katna1 digoxigenin-labelled sense and antisense riboprobes were synthetized from the linearised recombinant TOPO® TA cloning vector using T7 and T3 RNA Polymerase (Promega) according to the supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the GoTaq DNA Polymerase (Promega), followed by direct sequencing of the amplified fragment ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μL of the CellTiter-Glo 2.0 Cell Viability Assay kit (Promega, Cat# G9241) was added to the cells ...
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bioRxiv - Developmental Biology 2024Quote: ... using a spinning disk confocal microscope (see in vivo confocal imaging paragraph above) after embedding the embryos in 1% low-melting agarose (Promega, Cat# V2111) in PBS 1X in a glass bottom 60μ- Dish (35mm high ...
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bioRxiv - Cancer Biology 2024Quote: ... Glucose uptake was measured using the Glucose Uptake-Glo™ Assay (Promega, Cat # J1341) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Probes were synthetized using the manufacturer recommendation (T7 RNA polymerase, Promega, Cat# P2075 ...
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bioRxiv - Cancer Biology 2024Quote: ... inner salt] assay using CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega, Cat# G3582) at 72 h after BKIDCs were added for all cell lines ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then embedded on the side position in 1% low melting agarose (Promega, Cat# V2111) in a glass bottom 60μ-Dish (35mm high ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were on-bead digested with trypsin (Promega) at 37 °C for 16 h with orbital shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... cell proliferation was determined using CellTiter-Glo™ 3D Cell Viability Assay (Promega, Cat# G9683.) on GloMax® plate reader ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated in secondary antibody conjugated to horseradish peroxidase (Promega) at a dilution of 1:5000 for 1h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter-Glo (#G9242, Promega) was used to compare cell viability after H2O2 treatment quantitatively.
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bioRxiv - Cancer Biology 2024Quote: ... The assay was terminated with the addition of Kinase-Glo® Luminescent Kinase Assays reagent (Promega, Cat# V6711) and read in an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: ... The non-radioactive Kinase-Glo® Luminescent Kinase Assays (Promega, Cat # V6711) was used to measure ATP consumption in the presence of its natural substrate glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... Mastermix was prepared with GoTaq green (Promega, M7123) containing primers (final concentration 0.5 pmol/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was assessed using the CellTiter-Glo (Promega, Madison, WI) reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... RNasin (Promega) at 0.2U/µl and the donor minicircle DNA at 10ng/µl combined in microinjection buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was quantified by detection of ATP content in the culture medium with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The dsDNA was quantified by QuantiFluor dsDNA System (Promega, #E2671) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the fluorescence was measured by GloMAX (Promega) with 504 nm excitation and 531nm of emission ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells at 70-80% confluency were transfected with FuGENE HD Transfection Reagent (Promega) according to the supplier’s protocol.
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bioRxiv - Plant Biology 2024Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Analysis of GTP turnover was performed by using a modified protocol of the GTPase-GloTM assay (Promega) described previously74 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... medium and drug solutions were removed and 20 µl per well of BrightGlo reagent (Promega) was added ...
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bioRxiv - Physiology 2024Quote: ... Cysteine residues were alkylated in presence of 20 mM iodoacetamide at 37 °C in the dark for 30 min and tryptic digestion (sequencing grade, Promega) was performed at a 100:1 protein to enzyme ration at 37 °C overnight ...
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bioRxiv - Physiology 2024Quote: Total RNA was extracted from powdered whole heart tissue samples using the SV Total RNA isolation kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions or from hiPSC-cardiomyocyte extracts with the TRIzolTM reagent (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions (25 µL) were set up with GoTaq Green Master Mix (Promega, Madison, WI, USA), 200 nM of forward and reverse primers (Table S40) ...
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bioRxiv - Systems Biology 2024Quote: ... and firefly and renilla luciferase levels determined using the Promega Dual-Luciferase Reporter Assay System and a GloMax 96 Microplate Luminometer (Promega).
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bioRxiv - Physiology 2024Quote: ... chow diet fed mice using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). Reverse transcription was performed using a SuperScript™ IV First-Strand Synthesis System (Catalog no ...
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bioRxiv - Plant Biology 2024Quote: ... using GoTaq® qPCR Master MixA6001 (Promega, Madison, WI, USA) with the following protocol ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were first transfected with 3′UTR luciferase constructs (10 ng) using FuGENE HD transfection reagent (Promega) for 4 hours then transfected with Dharmacon miR-199a-5p mimic (50 nM ...
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bioRxiv - Systems Biology 2024Quote: ... into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Southampton, U.K.) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... the SW1353 cells were washed and lysed using Reporter Lysis Buffer (Promega) and firefly and renilla luciferase levels determined using the Promega Dual-Luciferase Reporter Assay System and a GloMax 96 Microplate Luminometer (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... All the key viability experiments were confirmed by the MTS assay (Promega, Catalog# G3582) and CCK-8 assay (APExBIO company ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from lymphoma derived cell lines as described above and PCR amplified using sgRNA specific primers (Supplementary Table 3, p53 primers as previously described (13) and GoTaq Green Master Mix (Promega). PCR products were amplified a second time using indexing primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lactate-Glo Assay (#J5021, Promega) and Glucose-Glo Assay (#J6021 ...
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bioRxiv - Cancer Biology 2024Quote: ... GSH/GSSG-Glo Assay (#V6611, Promega), according to the manufacturer’s instructions ...