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Citations for Promega :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplicons were generated in 50 µL reactions with GoTaq Green Master Mix (Promega) using the following PCR cycle ...
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bioRxiv - Cancer Biology 2024Quote: GSH/GSSG ratios were measured using GSH/GSSG-Glo™ Assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected and digested in 100 µl of Passive Lysis buffer (Promega, #E1941). The intracellular concentrations of ATP in the cultured cells were assayed using the ATP Determination Kit (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with CellTiterGlo reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RealTime-Glo (Promega) was added at 1x or 0.5x and after overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Cuboids were grown in 1 mL of collagen on a transwell with an air liquid interface and analyzed for viability and drug response using RealTime-Glo (Promega), followed by immunostaining ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µg of the specific lentiviral plasmid/construct using FugeneHD (Promega, E2311) in Optimem (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was measured using CellTiter-Glo 2.0 Luminescent Cell Viability Assay (Promega) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activity was assayed 48 hr after transfection using Dual-Luciferase Reporter Assay System (Promega). To measure enzalutamide IC50 ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay was performed according to the manufacturers protocol (Promega, Madison, WI) after 24 ...
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bioRxiv - Cancer Biology 2024Quote: ... or the Maxwell® RSC simplyRNA Tissue kit (Promega) were utilized ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated and DNAse I-treated using Maxwell® RSC simplyRNA Tissue kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20U/mL RNAsin Ribonuclease Inhibitors (Promega) for 10 minutes in ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega), relative light units (RLU ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiterGlo (Promega) was used to measure cell viability and dose-response curves were generated by non-linear fitting ...
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bioRxiv - Cell Biology 2024Quote: ... and minipreps (Promega) were used to purify DNA as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested after 24 h and luciferase activity was measured using the Luciferase Reporter Assay System (Promega, Cat. #E1500) on Victor Nivo luminometer (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA fragments were PCR-amplified with primer pairs containing a XhoI and BamHI site from human genomic DNA (Promega) and subcloned into the pCLL-NoPromoter-FLuc-CMV-RLuc-dsRed2 vector ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysis was achieved by adding 50 μL of Steady-Glo Luciferase Assay System buffer (Promega, E2510) to the wells ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection of HeLa cells with VPS35 plasmids was achieved using FuGene HD (Promega, #E2311) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested at 48 h post-transfection and activity of Firefly and Renilla luciferases were sequentially determined using the Dual-luciferase Reporter Assay System (E1980, Promega) and a Beckman Coulter microtiter plate reader (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Digestion was carried out using sequence-grade modified trypsin (Promega, Madison, WI) at an enzyme-to-protein ratio of 1:50 (w/w ...
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bioRxiv - Cancer Biology 2024Quote: ... after DNase I treatment with RQ1 RNase-Free DNase (Promega - M6101), reverse transcription was done using OneScript Plus cDNA Synthesis Kit (abm-G236) ...
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bioRxiv - Cancer Biology 2024Quote: ... with 200U/mL RNAsin (Promega) (3x pellet volume ...
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bioRxiv - Cancer Biology 2024Quote: ... with 200U/mL RNAsin (Promega) (3x pellet volume ...
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bioRxiv - Cancer Biology 2024Quote: ... Assay run in technical triplicate following manufacturer instructions (G8080, Promega).
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 µg of trypsin/Lys-C mixture (V5073, Promega) added to each on-bead sample and set to rotate head-over-head overnight at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Viability of tumor organoids was assayed using RealTime-Glo™ MT Cell Viability Assay by Promega with heme treated organoids compared to their baseline activity.
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bioRxiv - Cancer Biology 2024Quote: ... Instructions from Glutamine/Glutamate-Glo Kit (J8021, Promega) were followed past this point.
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bioRxiv - Cancer Biology 2024Quote: ... The cell viability was measured by adding MTS reagent (Cat. No. G-111, Promega, Madison, WI) according to the manufacturer’s protocol and as described previously.(26 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was tested after 96hrs of transfection using MTS kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a CFX96 q–PCR System using SYBR Green qPCR master mix (Promega, USA). GAPDH was used as the internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase levels were then detected using the Dual-Glo® Luciferase Assay System (Promega). Changes in luciferase levels were normalized to Renilla luciferase levels and compared to luminescence from the empty (no 3’UTR ...
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bioRxiv - Cancer Biology 2024Quote: ... and 50 U/ mL DNAse (# M6101, Promega) at 37°C for 1 hr rotating incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... ATP was quantified using CellTiter-Glo luminescent cell viability assay (Promega, G7570) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μl GoTaq Hot Start (Promega M5005) and 200 ng UMI-4C DNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was evaluated using CellTiter 96® Aqueous MTS assay (Promega; Madison, WI) as previously described [17] ...
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bioRxiv - Cancer Biology 2024Quote: We developed a high-throughput cytotoxicity plate based-assay using the Cell Titer-Glo® 2.0 Luminescent Cell Viability Assay (Promega, G9242) to screen CBCS Oncoset collection of 172 compounds with known targets (listed in table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μl MgCl 25 mM (Promega A3511), 0.2 mM dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was then diluted to 4 mL with 50 mM ammonium bicarbonate and subjected to overnight digestion at 37°C with trypsin (Promega) using an enzyme to substrate ratio of 1:200 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... and the CellTiter-Glo assay (Promega) was performed based on the manufacturer’s instruction to determine cell survival rate ...
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bioRxiv - Cancer Biology 2024Quote: ... The assessment of cell viability post-treatment was conducted using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). This experimental procedure was replicated across four biological replicates ...
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin was purchased from Promega. C18 SPE Cartridge was purchased from Waters (Milford ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed by measuring luminescence using CellTiter-Glo 2.0 Cell Viability Assay (Promega) in 2014 Envision multilabel reader (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplification of the target genes was performed using 2x green mix (Promega) with gene-specific primers following the manufacturer’s protocol ...