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Citations for Promega :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation, Madison, WI, USA), without fragment separation ...
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bioRxiv - Immunology 2024Quote: ... and development using NBT/BCIP substrate mix (Promega) diluted in AP buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... a Firefly Luciferase with two destabilization sequences has been used (Luc2CP, Promega), while for the biosensing experiments ...
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bioRxiv - Synthetic Biology 2024Quote: ... using 1 μL of unpurified template LET and 0.5 μL of FluoroTectTM (Promega) per 10 μL reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... Firefly Luciferase Arbitrary Units (Luciferase [A.U.]) were calculated by normalizing each sample’s Firefly Luciferase activity to the constitutive Renilla (pRL-TK, Promega) activity detected in the same sample ...
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bioRxiv - Synthetic Biology 2024Quote: Firefly Luciferase and Renilla Luciferase expression were measured following the protocol of Dual Luciferase Assay (Promega) on a Glomax Explorer plate reader (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... on a Glomax Explorer plate reader (Promega). The cells were collected 48 hours after transfection and lysed with 5X Passive Lysis Buffer (Biotin ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg of sequencing-grade trypsin (Promega) and 125 μL of digestion buffer (50 mM TEAB ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse IgG (Promega, 1:10,000), goat anti-rabbit IgG (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... and splice acceptor (SA)) were derived from the chimeric intron included in the pCI mammalian expression vector (Promega). We used a P2A sequence to prematurely truncate the SEED-target and facilitate expression of a transgene payload ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rabbit IgG (Promega, 1:20,000), goat anti-rat IgG + IgM (L+M ...
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bioRxiv - Synthetic Biology 2024Quote: ... All amplicons were then verified by gel electrophoresis and purified using a Wizard Gel Purification kit (Promega, Madison, WI).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were isolated from bacterial cells using PureYield Plasmid Miniprep System (Promega) or QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: Mammalian two-hybrid assays were performed following the instruction of the CheckMate™ Mammalian Two-Hybrid System (Promega). Constructs were synthesized by Genscript (Fig ...
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bioRxiv - Biochemistry 2024Quote: ... The FXR LBD (residues 247-476) and the hinge region (residues 197-243) were cloned into the mammalian Gal4DBD fusion vector pBIND (Promega), to give Gal4DBD-(FXR-LBD ...
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bioRxiv - Biochemistry 2024Quote: ... or PKA (Promega) kinases in phosphorylation buffer ...
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bioRxiv - Biochemistry 2024Quote: ... CK1 (Promega), or PKA (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Genetics 2024Quote: ... 2.5mM dNTP mix(Promega), ERCC RNA Spike-In Mix (1:3.2×107 Thermo Fischer scientific 4456740)) ...
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bioRxiv - Genetics 2024Quote: ... The sgRNA templates were transcribed using T7 polymerase with RiboMAX™ Large-Scale RNA Production Systems (Promega, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using Bright-Glo luciferase substrate (Promega) per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were transfected using Fugene (Promega) with a plasmid encoding the socRNAs of interest ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... luciferase activity was analyzed using the dual-luciferase reporter kit (Promega, USA).
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bioRxiv - Systems Biology 2024Quote: ... ROS levels were quantified by ROS-Glo™ H2O2 Assay (Promega, catalog # G8820) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Cell viability of bacterial cultures was measured using the BacTiter-Glo Microbial Cell Viability Assay (Promega) by detection of present ATP ...
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bioRxiv - Systems Biology 2024Quote: ... before adding 2 µL of 0.5 ng/µL trypsin (Promega) in digestion buffer (50 mM TEAB ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were collected at 2 dpi sprayed with a solution of 1 mM D–luciferin (Promega, E1603) and 0.02% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... amplified fragments were subcloned into the pGEM-T-Easy Vector (Promega), and 6 clones were sequenced ...
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bioRxiv - Plant Biology 2024Quote: ... for CDS of the FLC gene cloned into the pGEM®-T Easy vector (Promega, Madison, Wisconsin, US). Oligonucleotides and construct IDs are listed in Table S4.
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bioRxiv - Plant Biology 2024Quote: ... using Maxwell 16 LEV Plant RNA kit (Promega, USA). For each genotype/treatment combination ...
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... on a GM-2000 luminometer (Promega).
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bioRxiv - Pathology 2024Quote: ... at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega, VA1170) at 37 °C for 16 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... the T7 RiboMAX Large-Scale RNA Production System (Promega, Madison, WI, USA) was used and the synthesized mRNAs were purified with NucleoSpin RNA Plus (Takara) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and luminescence was measured using Bright-Glo substrate and lysis buffer (Promega) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Leaf tissue was collected from plants for high-molecular weight DNA extraction (Wizard HMW DNA Extraction, Promega, Madison, WI). We used flow cytometry to estimate genome size relative to Conyza canadensis and Solanum lycopersicum ...
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bioRxiv - Plant Biology 2024Quote: ... and subcloned into the pGEM-T® Easy Vector (Promega, Mannheim, Germany). PCR-related mutations were excluded by confirming that sequences of the cloned genes were identical to sequencing results from PCR products ...
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bioRxiv - Plant Biology 2024Quote: ... RNA purification was carried out using ReliaPrep™ Minicolumns following the manufacturer (Promega) protocol ...
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Comparative metagenomic profiling of seed-borne microbiomes in a landrace and a hybrid maize varietybioRxiv - Plant Biology 2024Quote: ... we used the Wizard Genomic DNA Purification Kit (Promega) followed by sequencing using the Sanger method.
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bioRxiv - Physiology 2024Quote: ... were seeded onto 12-well plates at 150.000 cells per well and transfected the next morning with 1μg of the BSEPprom-Luc or SHPprom-Luc plasmid and 100ng each of FXR and RXR expression plasmids using Fugene HD (Promega) at a ratio of 2.5:1 (reagent:DNA) ...
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bioRxiv - Physiology 2024Quote: Luciferase assays were performed using the Dual Luciferase reporter assay (Promega) according to the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and digested by trypsin (Promega V5280) at 1:100 w:w enzyme-to-sample ratio for each sample in 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2024Quote: ... the LUC and REN values were measured 24 hours later using the Dual-Luciferase® 80 Reporter Assay System (E1910, Promega, WI, USA)34 ...
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bioRxiv - Plant Biology 2024Quote: ... Rosette leaf samples were ground in liquid nitrogen and total RNA was extracted using the SV total RNA isolation system (Promega, www.promega.com). After quantification with a Nanodrop system (www.nanodrop.com) ...
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bioRxiv - Plant Biology 2024Quote: ... and DNase I was added to digest the genomic DNA (Promega, no. Z3100) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The blot was washed as before and then visualised with Attophos Substrate Kit (Promega, USA) using the Bio-Rad ChemiDoc XRS+ system.
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bioRxiv - Synthetic Biology 2024Quote: ... 1:50 (v/v) dilution of Nano-Glo substrate (Promega), and 10 ng/μL of DNA template from encoding the HiBit peptide64 (final volume of 8 μL) ...