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Citations for Promega :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The Dual-Glo Luciferase Assay System (Promega) was used to determine the relative activity of firefly luciferase to Renilla luciferase ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of the plasmid was co-transfected with 2.5µg of pGL4.75 Renilla luciferase control vector (Promega) into DT40 AIDR/puro UNG-/- cells ...
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bioRxiv - Microbiology 2024Quote: ... the target cells were incubated with 1:250 diluted EnduRen live cell substrate (Promega). After detachment ...
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bioRxiv - Microbiology 2024Quote: ... The Nano-Glo live cell reagent (Promega) was used as advised by manufacturer to determine effector translocation into RAW LgBiT macrophages ...
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bioRxiv - Microbiology 2024Quote: ... in vitro transcription was carried out using the Ribomax large-scale RNA production systems from Promega, and capping using the ScriptCap m7G capping system (Cellscript).
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity was assessed via the Renilla luciferase kit (Promega). In brief ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently Trypsin/LysC solution [100 ng/μL 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Microbiology 2024Quote: ... named rpsL*) was amplified by PCR with primers BlD-LLcfusARpsL/BlD-LLldacARpsL and cloned into the pGEM-T Easy vector (Promega), yielding plasmid pGEM-rpsL* ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Microbiology 2024Quote: HeLa cells were transfected with plasmids encoding ByeA or ByeAC197A according to the FuGENE transfection protocol (FuGENE® HD Transfection Reagent, Promega Cat. E2311) as advised by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... while the DNA was quantified using the GloMax Plate Reader System (Promega) using the QuantiFluor® dsDNA System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... we used the Nano-Glo HiBiT lytic detection system (Promega, Catv N3030). To determine the linear range of the luminescence signal we performed a dilution series with different numbers of bacteria ...
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bioRxiv - Microbiology 2024Quote: ... using the QuantiFluor® dsDNA System (Promega) chemistry ...
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bioRxiv - Microbiology 2024Quote: ... Each 25 µL reaction contained 1x GoTaq Colourless Master Mix (Promega, United States) and 0.3 µM Primers (invA_1869F ...
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bioRxiv - Microbiology 2024Quote: ... the nLuc activity of each antigen was adjusted to comprise 107 RLU per second (RLU/s) in a volume of 50 µL (NanoGlow, Promega, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from overnight cultures using Wizard® Plus SV Minipreps DNA Purification Systems (Promega, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Briefly, 100 µl elution buffer 1 (100 mM ammonium bicarbonate, 1 µg trypsin (Promega)) was added to each sample ...
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bioRxiv - Immunology 2024Quote: ... qPCR targeting the β-globin gene was performed for each sample and evaluated alongside a β-globin calibration curve (human gDNA, cat# G1521, Promega).
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bioRxiv - Immunology 2024Quote: DNA was extracted from 200 mg of each fecal sample via bead beating and column purification using a Maxwell 16 MDx Instrument (Promega, USA), as previously described (Sikder ...
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bioRxiv - Immunology 2024Quote: RNA from cells was isolated using the ReliaPrep RNA Miniprep System (Promega). RNA was reverse transcribed using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems ...
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bioRxiv - Immunology 2024Quote: Cell viability was determined by the CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7571), the Cell Counting Kit-8 (CCK-8 ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2024Quote: Total RNA extracted from BMDCs using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega, Madison, WI, USA) was reverse-transcribed to cDNA with ReverTra Ace qPCR RT Master Mix (#FSQ-201 ...
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bioRxiv - Immunology 2024Quote: ... A mix containing 1 µL of 10 mM dNTP (Promega), 5 µL of 0.25 µM NexteraXT primer index ...
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bioRxiv - Immunology 2024Quote: ... 0.4 µL of 10 mM dNTP mix (Promega), 0.4 µL of primers mix alpha ...
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bioRxiv - Immunology 2024Quote: ... One μg PCR product was used as the specific-template for synthesizing of dsRNA in vitro by using the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The concentration of dsRNA was quantified at 280 nm using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
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bioRxiv - Immunology 2024Quote: ... and the assay was performed using the Bio-Glo Luciferase Assay System (Promega). Additional controls include mock-(transfection with nuclease-free water ...
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bioRxiv - Immunology 2024Quote: ... Mek inhibitor U0126 (V1121) was obtained from Promega and re-constituted in DMSO (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... miiuy MDA5 gene was amplified using PCR with the gene-specific primers and ligated into a pmir-GLO luciferase reporter vector (Promega). Meanwhile ...
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA was extracted using a Tecan automation workstation (Promega). DNA whole-exome sequencing (WES ...
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... 1U of Taq DNA polymerase (Promega, Leiden, The Netherlands). Amplification was accomplished over 30 cycles as follows ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... using a GloMax® Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Microbiology 2024Quote: ... difficile strains carrying sLucopt reporter constructs was mixed with 20 µL of reconstituted Nanoglo substrate (50 fold diluted Nanoglo substrate in kit buffer, Promega) in a white ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were pre-digested with LysC (Promega) for 2 h at 25 °C and digested with trypsin (Promega ...
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bioRxiv - Microbiology 2024Quote: ... relative light units (RLU) were measured in a GloMax® Explorer Multimode Microplate Reader (Promega), using standard settings ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Digestion was performed using 10 ng/μl trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in purified CD8 T cell lysates with the luciferase assay system (Promega). Purified CD8 cells were lysed using cell lysis buffer 1X ...
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bioRxiv - Immunology 2024Quote: ... and total RNA extraction was performed (Promega) according to manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in native lysis buffer and luciferase activity was assayed using the Dual-Luciferase Reporter Assay (E1910, Promega) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... BrightGlo solution (Promega) was then added to the wells and luminescence was read on a SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Immunology 2024Quote: ... 120 µl of lysis buffer combined with Bright-Glo (Promega) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... DNA was combined with the transfection reagents FuGENE (Promega) and Transfectagro (Corning ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in Steady-Glo Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Firefly luciferase activity was measured after replacing the media with 50µl Bright-Glo luciferase reagent and measuring luminescence on a GloMax-MultiTm Detection system (Promega). Neutralization titers were calculated by interpolating the reciprocal serum dilution at which there was a 50% inhibition of luciferase activity relative to untreated controls (ND50).
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bioRxiv - Genomics 2024Quote: ... the RNase inhibitors (Promega, N2615 and Life Technologies, AM2696) were added to the lysis buffer ...