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Citations for Promega :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 200 U/ml RNAsin (Promega), 100 µg/ml cycloheximide and 1 mM DTT (Sigma-Aldrich)) ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out with oligo(dT) primers using Moloney murine leukemia virus reverse transcriptase according to the manufacturer’s instructions (Promega). The qPCR reaction was performed on an Applied Biosystems QuantStudio3 thermocycler using a SYBR™ Green PCR Master Mix (Thermo Fisher scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). The DNase treatment was performed according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (66) ...
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bioRxiv - Plant Biology 2024Quote: ... The Dual-Luciferase Reporter Assay System kit (Cat#E2940, Promega) was used to quantify fluorescence signals ...
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bioRxiv - Plant Biology 2024Quote: ... Dual luciferase assay reagents (Promega, VPE1910) with the Renilla luciferase gene as an internal control were used for luciferase imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... a Firefly Luciferase with two destabilization sequences has been used (Luc2CP, Promega).
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total RNA was purified of genomic DNA contamination using DNase (RQ1 RNAse-free DNAse, Cat. # M610A, Promega, Madison, WI) and reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Cat.# 4368814 ...
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bioRxiv - Systems Biology 2024Quote: ... Sequencing-grade modified trypsin was from Promega.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Trypsin digested peptides were diluted to 200 μL with final concentration of 100 mM NH4HCO3 and 0.02% ProteaseMAX Surfactant prior to the addition of sequencing grade chymotrypsin (Promega) to a final 1:20 protease:protein ratio (w:w) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequencing grade-modified trypsin (Promega) was added to a final 1:20 protease ...
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bioRxiv - Systems Biology 2024Quote: ... 50 µL of Cell Titer Glo reagent (Promega) was added to each well and incubated for 10 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The samples were further digested overnight at 37 °C with the addition of sequencing grade chymotrypsin (Promega) to a final protease:protein (w:w ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bands were dehydrated and dried by speed vac before resuspension in 100 μL 0.2% ProteaseMAX™ surfactant (Promega) and 50mM NH4HCO3 solution containing trypsin (sequencing grade ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 50mM NH4HCO3 solution containing trypsin (sequencing grade, Promega) at a 1:20 protease:protein ratio (w:w) ...
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bioRxiv - Synthetic Biology 2024Quote: ... to prevent nicking of the DNA and its purification was carried out using the ReliaPrep™ DNA Clean-Up and Concentration System (Promega, USA) according to manufacturer specifications ...
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bioRxiv - Neuroscience 2024Quote: ... Anesthetized zebrafish were embedded in low melting point agarose gel (Promega, V2111) in a confocal dish ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 80μL Hank’s balanced salt solution (HBSS) containing 10mM HEPES and 10μM furimazine/NanoGlo Luciferase Assay Substrate (Promega). BRET measurements were performed at 37 °C using a PHERAstar Microplate Reader (BMG Labtech ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Zoology 2024Quote: ... 1 μl 10mM dNTPs (Promega), 2.5 μl of each primer at 10 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by luminescence detection using GloMax Explorer (Promega). The bioluminescence readings were averaged amongst the n=3 technical replicates for each construct and ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... or using Caspase-Glo® 8 Assay Systems® (Promega, G8200) to measure cleaved Caspase 8 activity.
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Systems Biology 2024Quote: ... Cell viability was then quantified using CellTiter-Glo (Promega, catalog # G7572) according to the manufacturer protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Cell lines were routinely tested for mycoplasma and were periodically authenticated to its early passage using the GenePrint 10 System (Promega, catalog # B9510). VEM and DAS (Selleck Chemicals ...
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bioRxiv - Systems Biology 2024Quote: ... then samples were incubated with sequencing-grade modified trypsin (Promega, catalog # V5113) for 18 hours at a trypsin:protein ratio of 1:50 (w/w) ...
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bioRxiv - Systems Biology 2024Quote: ... 2.5 μL of Express Mix (T7 RiboMAX; Promega), and an appropriate volume of nuclease-free water to achieve a final reaction volume of up to 25 μL ...
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bioRxiv - Biophysics 2024Quote: ... Cells were grown to 70-80% confluency before transfection with β1AR constructs using FuGENE HD reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Then Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega) was used to extract FFPE DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was quantitated using the CellTiter-Glo 3D Cell Viability Assay (G9681, Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... with 10ul/ml rRNasin (Promega) and Superasin (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Samples were dehydrated again as before and digested with sequencing grade modified trypsin (Promega) shaking overnight at RT ...
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bioRxiv - Immunology 2024Quote: ... luciferase signal was assayed using Nano-Glo Luciferase assay system (Promega) and the signal for each mAb tested was normalized to a no-antibody control.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the treatment was removed from the wells and the cells were incubated with 100 μl of EGM™-2 complete medium with 20 mM HEPES and 20 μl of MTS (Promega, CellTiter 96® AQueous One Solution Reagent ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... RNase inhibitor (N2615) was from Promega, RNase A (EN0531) ...
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bioRxiv - Immunology 2024Quote: ... sourced from the Promega Nano-Glo Luciferase Assay kit (Promega, N1110), with a substrate to buffer ratio of 1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Mouse antibodies (CV801, CV804, CV820, CV925, CV1117) and effector cells (Promega) expressing mouse FcγRIV and NFAT luciferase reporter were added to the cells ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were reverse transfected using Lipofectamine RNAimax Transfection Reagent (Promega) 6-well plates for 24 hours prior to splitting to final destination plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed after five days using CellTiter-Glo 3D (Promega G9683), with luminescence measured ...
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bioRxiv - Biophysics 2024Quote: ... Furimazine (or NanoGlo substrate) was obtained from Promega (Cat #N2012). FrameStar® 384 Well Skirted PCR plates (#4ti-0385 ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Plant Biology 2024Quote: ... The pIX-HALO Vector (cat#G184A, Promega) was used to fuse the GhDREB2A and GhHSFA6B into the HaloTag ...
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bioRxiv - Plant Biology 2024Quote: ... Magens HaloTag Beads (G7281, Promega) was used to purify the fusion protein ...
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bioRxiv - Plant Biology 2024Quote: ... We further used the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) to express the four TFs-HaloTag fusion protein ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... distilled water) containing 0.1% NP40 and 0.1% Tween-20 and 0.01% Digitonin (Promega, G9441) and incubated for 3 min to allow cells permeabilization ...
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bioRxiv - Biophysics 2024Quote: cDNAs for human Cx26 and Cx30 were synthesized and subcloned by Epoch Life Science into pGEM-HA (Promega), or pcDNA 3.1+ (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Tat and Rev and plasmid encoding spike of SARS-CoV-2 variants Delta or Omicron were transfected in HEK 293T cells using Fugene6 reagent (Cat. No. E2691, Promega) following manufacturer’s guidelines ...