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Citations for Promega :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... using ADP-Glo Kinase Assay (Promega). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... NLuc-N112C-CCNE1-3xFLAG plasmids were cloned into PvuI and EcoRI digested NLuc-CDK4 plasmid (Promega) using gBlocks obtained from IDT ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Biochemistry 2024Quote: ... samples were digested using MS-grade trypsin (Promega) at 37 ℃ overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Normalized cell lysate was first mixed with vivazine (Promega) to reach 1% vol/vol as recommended by Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The expression plasmids were transiently transfected by using FuGENE HD (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA was purified using the Wizard SV Gel and PCR Clean Up Kit (Promega). Immunoprecipitated DNA was analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell growth inhibition was determined using Cell Titer Glo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The GoScript Reverse Transcriptase System (Promega, Madison, WI) was used to synthesize the first strand cDNA from 1μg of the extracted RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The growth inhibition was quantified using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (Promega) and the Incucyte SX5 Live-Cell Analysis System (Sartorius) ...
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bioRxiv - Cancer Biology 2024Quote: ... and the CellTiter-Glo assay (Promega) was then performed according to the manufacturer’s instructions to determine cell proliferation ...
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bioRxiv - Cancer Biology 2024Quote: ... reflecting cell-number and –viability was assessed using the CellTiter-Glo® 3D Cell Viability Assay (Promega, G9682) and sigmoidal dose-response curves were generated to calculate the mean IC50 values of each drug that were used in the subsequent study ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activity was quantified using a dual luciferase assay kit (Promega). Relative luciferase activity was calculated by normalizing relative luciferase activities to Renilla activities in each well.
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bioRxiv - Cancer Biology 2024Quote: ... Baseline CellTiterGlo (Promega) reading was taken at specified time points.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed at 72 h post-treatment by performing CellTiter-Glo Luminescent Cell Viability Assays (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were washed twice with ice-cold ethanol followed by overnight incubation at 37°C in 100 mM ABC containing 1 mM CaCl2 and trypsin (Sequencing grade, modified, Promega Co, Madison, WI, USA). Digested proteins were evaporated and each sample was freed from salts and buffers by solid-phase extraction on C18 resin using cartridges prepared in-house ...
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bioRxiv - Cancer Biology 2024Quote: The apoptotic effect of MK-1775 was determined by means of caspase 3/7 activity via Apotox-Glo Triplex Assay (Promega, #G6320) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... using Enduren (Promega Corporation, Madison, WI) as the substrate for Renilla luciferase.
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bioRxiv - Cell Biology 2024Quote: ... or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega, G8471). After receptor labeling ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μg of dsRNA was transfected into 1x106 D-Mel cells using Transfast transfection reagent (#E2431, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Janelia Fluor 549 (JF549) or JF646 ligand (Promega GA1110 or GA1120) was added to media to a final concentration of 15 – 50 nM and incubated for 15 – 30 min or indicated time ...
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bioRxiv - Cell Biology 2024Quote: ... purchased from Promega in Madison ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted into the appropriate locations of pGL4.12 (Promega, Madison, Wisconsin, USA) to create the pGL-TK minimal promoter reporter plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... and a CMV-Renilla (pGL4.75[hRluc/CMV], Promega) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then lysed 24 hours after treatment and luciferase activity was measured using the Dual Luciferase Assay (E1910, Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A2-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... at 5 µM in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 5 µL per well of 20x-NanoBRET Tracer K10 (Promega) at 5 µM in Tracer Dilution Buffer (Promega N291B ...
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bioRxiv - Cell Biology 2024Quote: ... Gel pieces were destained and in-gel trypsin digested (Promega, V5280). The peptide mixture was purified on PepClean C18 spin columns (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: ... samples were digested using trypsin enzyme (Promega) overnight at 37 °C at pH 8 in 50 mM ammonium bicarbonate buffer ...
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bioRxiv - Biophysics 2024Quote: ... Ninety microliters of the oocyte supernatant were added to 40 µl of luciferase-luciferin solution (Promega, Madison, WI) for assaying luciferase activity ...
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bioRxiv - Biophysics 2024Quote: ... HRP conjugate (Promega, W4021) as the secondary antibody.
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bioRxiv - Biophysics 2024Quote: ... Cells transduced with MCP-HALOnls were incubated for 24 hours before overnight staining with HaloTag-JF646 (Promega, #GA1120) at 10 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... transfected with 8 µg of pLib_EF1A_LuciNeo (kindly provided by Ralf Graeser, ProQinase, Freiburg) or pLib_EF1A_nlstdTomato-2A-puro using FugeneHD (Promega). Medium was exchanged after 4-8 h ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg of RNA was reverse-transcribed using M-MLV reverse transcriptase (Promega) with random hexamer primers (Amersham).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in 50 mM TEAB containing trypsin (Promega) at a 1:20 enzyme-to-protein ratio overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The cell viability was assessed using the Celltiter-Glo kit (Promega) through measurement of the luminescence signal on a Polar Star Optima plate-reader (BMG Labtech ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 µL of Steady-Glo Luciferase Reagent (Promega, E2510) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: Three feeding late-third-instar larvae for each sample were collected into Glo Lysis Buffer (Promega, E2661) in 2-mL Eppendorf tubes ...
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bioRxiv - Cell Biology 2024Quote: ... Histones were then digested with 1 µg of sequencing grade trypsin (Promega) diluted in 50 mM ammonium bicarbonate (1:20 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µL of CellTiter Glo reagent (Promega, Madison, WI) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... All plasmid cDNA transfections were performed using the FuGENE HD Transfection Reagent (E2311; Promega) in Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using GoTaq qPCR Master Mix (Promega, Madison, WI, USA) with the StepOne Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... using a Griess assay (Griess Reagent System; Promega) [21].
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bioRxiv - Cell Biology 2024Quote: ... rabbit (CST 7074S) or chicken (Promega G135A) in TBST containing 1% BSA for 1 hour at room temperature on shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 0.75 µg trypsin (Sequencing Grade Modified Trypsin, V5111, Promega) overnight at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed with CellTiter Glo (Promega). Surviving fractions at the two radiation doses were calculated and compared.