Labshake search
Citations for Promega :
601 - 650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... Cells were stained with 10 nM Janelia Fluor® 549 (JF549) HaloTag Ligand (Promega, Madison, WI) for 30 min and were washed with the medium at least three times ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells with HaloTag-Sec61 were stained with 200nM of JaneliaFluor 646 (JF646, Promega) for 30min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with the Ribo m7G Cap Analog (Promega), as described previously41 ...
-
bioRxiv - Cell Biology 2024Quote: mRNA was synthesized in vitro with linearized template plasmids using the RiboMax Large Scale RNA Production System-T7 (Promega) supplemented with the Ribo m7G Cap Analog (Promega) ...
-
bioRxiv - Cell Biology 2024Quote: RAW 264.7 cells were transfected using FuGene HD (Cat# E2311, Promega) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... and then cells were homogenized according to the manufactureŕs instructions (Dual-Luciferase resporter assay system, Promega, Madison, WI, USA)
-
bioRxiv - Cell Biology 2024Quote: ... samples were treated with RNase-Free DNase Kit (#M6101; Promega), quantified ...
-
bioRxiv - Cell Biology 2024Quote: ... Modified porcine trypsin (Promega, Madison ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were measured for ATP content using the Enliten ATP assay kit (Promega, FF2000) and following the instructions provided by the manufacturer.
-
bioRxiv - Cell Biology 2024Quote: ... a sample of the supernatants was removed for ATP measurement using the Enliten ATP assay kit (Promega, FF2000), following the instructions provided by the manufacturers ...
-
bioRxiv - Cell Biology 2024Quote: ... a 2456 bp DNA fragment of Ccl5 enhancer region and 1500 bp Ccl5 promoter were cloned into a pGL3-basic (purchased from Promega) vector between MluI and HindIII sites.
-
bioRxiv - Cell Biology 2024Quote: ... then incubated with Halo-TMR ligand (Promega) for 1 hour at 37°C with shaking at 500rpm ...
-
bioRxiv - Cell Biology 2024Quote: ... luciferase expression was measured using the Bright-Glo luciferase assay system (Promega, E2650).
-
bioRxiv - Cancer Biology 2024Quote: ... cell viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... CellTiter-Glo 2.0 (#G9243, Promega, Madison, Wisconsin, USA) was added to each well in a 1:1 v/v ratio ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2 ng of Renilla luciferase expressing pRL SV40 plasmid (E2231, Promega), as internal control ...
-
bioRxiv - Genetics 2024Quote: ... and DNase (Promega, M6101) for 15 min each at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... genomic DNA from cell pellets were extracted using the Wizard Genomics DNA Purification Kit (Promega) and quantified using the Qubit dsDNA Broad Range Assay kit (Invitrogen) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and random hexamers (Promega). Quantitative real-time RT-PCRs were performed in a QuantStudio 7 Flex System (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation and 200 µL of supernatant was incubated for 20 min at 21°C with 5 µg of RNA in 200 µL of 2xTENT buffer (100 mM Tris pH 7.8, 2 mM EDTA, 500 mM NaCl, 0.1% Tween) supplemented with RNAsin (Promega). RNA-protein complexes were then incubated with MyOne Streptavidin T1 DynaBeads (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... reagents from RealTime-Glo™ Annexin V Apoptosis Assay (Promega) were added to the culture ...
-
bioRxiv - Genetics 2024Quote: Total cholesterol and cholesterol esters were measured using the Cholesterol Ester-Glo Assay (Promega cat # J3190) using the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... Dual luciferase assay reagents (Promega, VPE1910) with the Renilla luciferase gene as an internal control were used for luciferase imaging ...
-
bioRxiv - Genetics 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were then separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
-
bioRxiv - Genetics 2024Quote: ... 5 µL of GoTaq G2 Green Master Mix (Promega), and 200 µM of primers ...
-
bioRxiv - Genetics 2024Quote: ... and 10 µL of power SYBR green PCR master Mix (Promega). The real-time qPCR was performed using a fluorescent quantitative detection system (FQD-96A ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
-
bioRxiv - Molecular Biology 2024Quote: ... Trypsin/Lys-C (Promega) solution was added ...
-
bioRxiv - Molecular Biology 2024Quote: Biotinylated RNA probes were produced by in vitro transcription using T7 RNA polymerase (Promega), and by adding 1:10 CTP-biotin analog:CTP to the reaction mix ...
-
bioRxiv - Genetics 2024Quote: ... We prepared triplicate tissue homogenates from ten F1 progeny from each cross using the Luciferase Cell Culture Lysis 5X Reagent (Promega) to extract total proteins by following the quick-freeze homogenization method outlined by the manufacturer ...
-
bioRxiv - Molecular Biology 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the QuantiFluor dsDNA System (Promega Corp.). Paired end ...
-
bioRxiv - Molecular Biology 2024Quote: ... RT-qPCR was performed using SYBR Green PCR Master Mix (A6002, Promega) with the gene-specific primers (Table S9) ...
-
bioRxiv - Molecular Biology 2024Quote: The cytotoxicity of venoms was assessed in Madin-Darby canine kidney II (MDCK II) cells using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, cat. no. G7570) as previously reported (Hurka et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
-
bioRxiv - Microbiology 2024Quote: ... Trypsin Gold was purchased from Promega (Madison, WI). Hydrophilic–lipophilic balance (HLB ...
-
bioRxiv - Microbiology 2024Quote: ... trypsin digestion was performed after diluting urea to <1.2 M and using 0.5 µg trypsin (Promega), and processed peptides were dissolved in 20 µl of buffer A ...
-
bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
-
bioRxiv - Microbiology 2024Quote: ... 250 and 560 μg/mL of G418 (Promega, Madison, WI, USA) was added to the culture medium ...
-
bioRxiv - Microbiology 2024Quote: ... an inhibition test was performed by mixing the soil DNA extracts with a control plasmid DNA (pGEM-T Easy Vector, Promega, Madison, WI, USA) or water ...
-
bioRxiv - Cell Biology 2024Quote: ... Sequencing grade trypsin (Promega) was added 1:50 enzyme:protein for 2 h at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
-
bioRxiv - Microbiology 2024Quote: ... the extracted RNA was reverse transcribed by using the reverse transcription system (Promega Corporation, Fitchburg, WI, USA). For this ...
-
bioRxiv - Microbiology 2024Quote: The denatured protein samples from co-immunoprecipitation were run into stacking gel and the sample gel band stained by Coomassie Brilliant Blue G250 were digested by trypsin (V5280, Promega) with prior reduction and alkylation in 50 mM ammonium bicarbonate at 37°C overnight ...
-
bioRxiv - Microbiology 2024Quote: Final library purification was performed using the ProNex Size-Selective DNA Purification System (Promega, NG2001) and eluted in water before libraries pooling and sequencing using a paired-end 150 method on a Illumina NovaSeq System by Novogene.
-
bioRxiv - Microbiology 2024Quote: ... on extracted genomic DNA (Promega Wizard DNA Extraction) testing 3 amplifications using primers P4 and P5 ...
-
bioRxiv - Microbiology 2024Quote: ... followed by treatment with RNAse (Promega, 0.3 μg/μl). Total genomic DNA was then extracted through phenol-chloroform extraction and ethanol precipitation ...
-
bioRxiv - Microbiology 2024Quote: ... or directly used with the ONE STEP TAQ QPCR kit (Promega). For RT-qPCR assays ...
-
bioRxiv - Developmental Biology 2024Quote: ... Luciferase assays were performed and measured using the Dual Luciferase Assay System (Promega). Expression was calculated as the ratio between the firefly and Renilla luciferase activities ...
-
bioRxiv - Microbiology 2024Quote: ... and 0.5 µL RNasIN (Promega Cat#N2511) at 37°C/30m ...