Labshake search
Citations for Promega :
551 - 600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ADP-Glo™ (Promega) was performed according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Crosslinks were reversed by addition of 2 uL Proteinase K (Promega) and incubation at 65 °C for 12-16 hours in a Thermomixer ...
-
bioRxiv - Genetics 2024Quote: 2000ng DNA (fixed or non-fixed) was mixed with 10μL Brilliant III SYBR Green qPCR Master Mix (Promega) and the DNA melting behaviour was analysed in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Genetics 2024Quote: The cell viability was measured every 24 hrs using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, #G4100) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The viability was measured by using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7572).
-
bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was used to amplify the OGDH 3’UTR and sub clone it into pmirGLO vector (Promega, UK). 5 × 10^4 C2C12 cells were seeded into 12 well plates ...
-
bioRxiv - Neuroscience 2024Quote: ... 50 mM triethylammonium bicarbonate and 1 μg of Trypsin/Lys-C mix (Promega) were added ...
-
bioRxiv - Microbiology 2024Quote: Final library purification was performed using the ProNex Size-Selective DNA Purification System (Promega, NG2001) and eluted in water before libraries pooling and sequencing using a paired-end 150 method on a Illumina NovaSeq System by Novogene.
-
bioRxiv - Microbiology 2024Quote: ... on extracted genomic DNA (Promega Wizard DNA Extraction) testing 3 amplifications using primers P4 and P5 ...
-
bioRxiv - Microbiology 2024Quote: ... followed by treatment with RNAse (Promega, 0.3 μg/μl). Total genomic DNA was then extracted through phenol-chloroform extraction and ethanol precipitation ...
-
bioRxiv - Microbiology 2024Quote: ... or directly used with the ONE STEP TAQ QPCR kit (Promega). For RT-qPCR assays ...
-
bioRxiv - Microbiology 2024Quote: ... Luminescence was quantified using the Nano-Glo Substrate (Promega) and measured in a GloMax Explorer (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... Luminescence in supernatants and cell lysates was determined using the Nano-Glo Substrate (Promega) and measured in a GloMax Explorer (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... and measured in a GloMax Explorer (Promega) luminometer ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were examined using the Dual-Glo® Luciferase Assay System (Promega, E2920) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2024Quote: ... Kanamycin-sensitive exconjugants were screened for the mutant with colony PCR using altgenoF and altgenoR primers designed outside of the deletion flanks with the following PCR reaction mix: 10 µl of GoTaq Green master mix (Promega), 0.5 µl of each primer at 10 µM concentration ...
-
bioRxiv - Microbiology 2024Quote: ... and FuGENE HD (Promega, Madison, WI) per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Overnight digestion was performed by adding trypsin (ratio 1:50, Promega) at 37°C while shaking ...
-
bioRxiv - Microbiology 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Peptides were separated on an Acquity BEH C18 column (100 x 1.0 mm ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µL of extracted DNA was added to an amplification mixture containing 1 µl (10 µM) of primers and 12.5 μl of PCR Master Mix Plus (Promega, USA). To each mixture ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.125 μl dNTPs mix (100 mM, Promega), 0.25 μl 5× Q5 Polymerase Buffer (NEB #M0491) ...
-
bioRxiv - Cell Biology 2024Quote: ... HaloTag TMR ligand (5mM, Promega, #G8251); LIPIDURE (0.2% NOF ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
-
bioRxiv - Cell Biology 2024Quote: ... a solution containing 50 nM JF549-HTL (Promega GA1110) was applied for 15 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... alternative HaloTag labeling using Alexa660-HTL (Promega G8471) at 3.5 µM concentration did not generate a sufficient labeling outcome.
-
bioRxiv - Molecular Biology 2024Quote: ... 500 ng of sequencing grade trypsin (Promega) were added for digestion carried out overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10X Primer extension buffer and 2 U AMV Reverse Transcriptase (Promega) were added to the annealing mixture and incubated for 1 hour at 42 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA samples (from three independent plants in all cases) were subjected to reverse transcription with the AMV enzyme (Promega) and random hexanucleotides as primers ...
-
bioRxiv - Microbiology 2024Quote: ... the extracted RNA was reverse transcribed by using the reverse transcription system (Promega Corporation, Fitchburg, WI, USA). For this ...
-
bioRxiv - Microbiology 2024Quote: LDH in neutrophil supernatants was quantified using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega®) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the isolates using the Wizard Genomic DNA purification kit (Promega Corporation, Madison, WI) and the final concentration was measured with a Qubit 3.0 Fluorometer (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: Cell viability post-drug treatment was measured via both MTT or WST assays according to manufactorer’s protocol (Promega) and automatic cell counter measurements (Luna II automatic cell counter) ...
-
bioRxiv - Microbiology 2024Quote: Real-time RT-qPCR assays (GoTaq 1-step qRT-PCR, Promega) were performed using standard fast cycling parameters (i.e ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was incubated with HaloLink Resin (Promega) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... the resin crosslinked with Halo-Nwd1 was incubated with HaloTEV protease (Promega) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... a radioactive probe was made using 50 ng of linearized pCtrl and the Prime-a-Gene Labeling System (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and chromosomal DNA was obtained using the Wizard® Genomic DNA Purification Kit (Promega). DNA fragments were purified from agarose gels using the Zyppy gel extraction kit ...
-
bioRxiv - Microbiology 2024Quote: RNA (500 ng) was treated with RQ1 DNase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
-
bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
-
bioRxiv - Microbiology 2024Quote: ... Firefly and Renilla luciferase production were evaluated using the Dual Luciferase Reporter Assay System (Promega Cat#E1910) and the Multimode Plate Reader (Molecular Devices).
-
bioRxiv - Microbiology 2024Quote: Intracellular ATP levels were measured using BacTiter-Glo Microbial Cell Viability Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... and absorbance measured at 490nm using a spectrophotometer (Glomax Multi+; Promega, UK).
-
bioRxiv - Microbiology 2024Quote: ... Fragment ends were repaired using T4 DNA Polymerase (M4211, Promega, Madison, Wisconsin), Klenow DNA Polymerase (KP810250 ...
-
bioRxiv - Microbiology 2024Quote: ... The only exceptions being the use of 2.5 mL of 10x FastBreak Cell Lysis Reagent (Promega) to lyse the 1 L cell pellets and the Gel Filtration Buffer (25 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2024Quote: In vitro transcription and translation reactions using rabbit reticulocyte lysates as previously described (29) were performed by TNT Quick Coupled Transcription/Translation System (Promega) in the presence of [35S] Met (Perkin-Elmer) ...
-
bioRxiv - Microbiology 2024Quote: ... GSH and GSSG were quantified using the luminescence-based GSH-Glo assay kit (Promega). GSH standards of 0.5 – 20 µM were quantified to determine the linear range of luminescence readings ...
-
bioRxiv - Microbiology 2024Quote: Correct integration of the homology template at the desired location was assessed by PCR (GoTaq DNA Polymerase, Promega) on extracted genomic DNA (Promega Wizard DNA Extraction ...
-
bioRxiv - Cell Biology 2024Quote: Genotyping was performed by PCR in 20 µl volume reactions that included 1x GoTaq Green Mas-ter Mix (Promega #M7123), 1.1 mM MgCl2 ...