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Citations for Promega :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Janelia Fluor 549 (JF549) or JF646 ligand (Promega GA1110 or GA1120) was added to media to a final concentration of 15 – 50 nM and incubated for 15 – 30 min or indicated time ...
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bioRxiv - Cell Biology 2024Quote: ... purchased from Promega in Madison ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted into the appropriate locations of pGL4.12 (Promega, Madison, Wisconsin, USA) to create the pGL-TK minimal promoter reporter plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... and a CMV-Renilla (pGL4.75[hRluc/CMV], Promega) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then lysed 24 hours after treatment and luciferase activity was measured using the Dual Luciferase Assay (E1910, Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A2-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... at 5 µM in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 5 µL per well of 20x-NanoBRET Tracer K10 (Promega) at 5 µM in Tracer Dilution Buffer (Promega N291B ...
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bioRxiv - Cell Biology 2024Quote: ... Gel pieces were destained and in-gel trypsin digested (Promega, V5280). The peptide mixture was purified on PepClean C18 spin columns (Thermo Scientific ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... Cells were stained with 10 nM Janelia Fluor® 549 (JF549) HaloTag Ligand (Promega, Madison, WI) for 30 min and were washed with the medium at least three times ...
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bioRxiv - Cell Biology 2024Quote: ... Modified porcine trypsin (Promega, Madison ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were measured for ATP content using the Enliten ATP assay kit (Promega, FF2000) and following the instructions provided by the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... a sample of the supernatants was removed for ATP measurement using the Enliten ATP assay kit (Promega, FF2000), following the instructions provided by the manufacturers ...
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bioRxiv - Cell Biology 2024Quote: ... a 2456 bp DNA fragment of Ccl5 enhancer region and 1500 bp Ccl5 promoter were cloned into a pGL3-basic (purchased from Promega) vector between MluI and HindIII sites.
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bioRxiv - Cell Biology 2024Quote: ... then incubated with Halo-TMR ligand (Promega) for 1 hour at 37°C with shaking at 500rpm ...
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bioRxiv - Cell Biology 2024Quote: ... luciferase expression was measured using the Bright-Glo luciferase assay system (Promega, E2650).
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bioRxiv - Cell Biology 2024Quote: ... or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega, G8471). After receptor labeling ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μg of dsRNA was transfected into 1x106 D-Mel cells using Transfast transfection reagent (#E2431, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (E1910, Promega corporation, Madison, WI), and subsequently used to infect suspension cultures of Sf9 cells (2 × 106 cells/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral replication was measured from supernatants collected 24 hrs post infection and viability determined at 48 hrs by 3D CellTiter-Glo assay following the manufacturer’s instructions (Promega).
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed to cDNA using random primers and GoScript Reverse Transcriptase kit (Promega). RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Biophysics 2024Quote: ... Promega Chemiluminescent Substrate (Promega) was used according to the manufacturer’s instructions to visualize TTR content.
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Genetics 2024Quote: ... total RNA was isolated from the transfected cells using Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA and reverse transcription was performed using the GoScript™ Reverse Transcription System (Promega, Madison, MI, USA). PCR amplification was performed using the pCAS2-RT-F and pCAS2-RT-R primers ...
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bioRxiv - Genetics 2024Quote: ... cell viability was determined using CellTiter-Glo Luminescence Cell viability Assay kit (Promega) by measuring luminescence (ATP level ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged CXCR2/CXCR3/CXCR7 and 3.5μg of F22 (Promega, Cat. no: E2301). 14-16h post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... Quenchbody PCR products were purified using Wizard Clean up kits (Promega; as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Bioengineering 2024Quote: ... A pHR expression plasmid and lentiviral packaging plasmids were co-transfected using the Fugene HD transfection reagent (Promega). 52 hours after transfection ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... emulsions of octanol + 5% (w/v) Span 80 + 40 µM Nile Red in an aqueous phase of nuclease free water (Promega) + 200 ng/µL miniprepped plasmid + 5% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... a released luciferase assay was performed using the luciferase assay system (Promega). Medium was removed and cells were washed with 100 uL of PBS ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... followed by the addition of ADP GloTM Kinase detection reagent (Promega #V6930) to convert ADP to ATP and introduce luciferase to detect ATP ...
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bioRxiv - Bioengineering 2024Quote: ... cells were lysed with Reporter Lysis Buffer (Promega, Madison, WI, USA, cat.: E3971) and freeze-thawed at −80°C ...
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bioRxiv - Biochemistry 2024Quote: ... Then membranes were incubated with secondary anti-rabbit IgG horse radish peroxidase (Promega, 1:5000) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: The ADP-Glo™ Kinase Assay kit (V9104) was purchased from Promega Corporation (Madison ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin Golden (Promega, USA), dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 100-fold diluted NanoBRET™ Nano-Glo® Substrate (Promega) was added and donor emission at 450 nm and acceptor emission at 618 nm were read within 10 min of substrate addition using a ClarioStar plate reader (Mandel Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... +/- 1000-fold diluted HaloTag® NanoBRET™ 618 Ligand (Promega) and incubated with indicated compounds concentrations or DMSO control for 4 h ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) was used to digest proteins at 37 °C for 16 h.
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bioRxiv - Biochemistry 2024Quote: ... Samples were lyophilized and dissolved in digestion buffer (50 mM ammonium bicarbonate [ABC], 1 mM calcium chloride) and digested overnight using trypsin (Promega, #V5117) at trypsin ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2024Quote: ... Tryptic digestion was performed on-bead with 1.5 μg of sequencing grade trypsin (Promega Corp.) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: The ADP-Glo™ assay (Promega) was performed according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). RT-qPCR primers and probes were listed in Supplementary Data 2.
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bioRxiv - Cancer Biology 2024Quote: ... the GSH/GSSG-Glo Assay (Promega) was performed following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction system qPCR Master Mix (GoTaq®, Promega) was utilized with the following specific protocol described by the company ...
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bioRxiv - Biochemistry 2024Quote: ... Nano-Glo® Live Cell Substrate (Promega) was added to all wells ...
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bioRxiv - Biochemistry 2024Quote: ... The proteases trypsin and Lys-C (Promega, V5073) were used to digest the LtEc proteins overnight at 37°C after reduction with 5 mM TCEP at 55°C and alkylation with 20 mM iodoacetamide at room temperature ...