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Citations for Promega :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Protein A biosensors (GatorBio) were equilibrated for 20 min in BLI buffer (PBS buffer supplemented with 0.02% tween-20 (Promega) and 0.1% bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: Ten microliters of supernatant from all the above reactions were incubated with 10 µl of freshly prepared UDP nucleotide detection reagent (Promega, Cat#: V6961) according to the manufacturer’s specifications ...
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bioRxiv - Bioengineering 2024Quote: ... cell viability was quantified using the CellTiterGlo Luminescent Cell Viability Assay (Promega, Walldorf, Germany). Luminescence was measured in black 96-well plates in a Synergy H4 microplate reader (Biotek ...
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bioRxiv - Bioengineering 2024Quote: Coupled transcription-translation assays were carried out using the TNT SP6 Quick Coupled transcription/translation system (Promega). Reactions were assembled containing 8 μl TnT Quick Master Mix ...
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bioRxiv - Bioengineering 2024Quote: ... RNAP sequences were synthesised and cloned into XhoI and XbaI restriction sites on the pTNT vector (Promega). To create the corresponding transcription templates ...
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bioRxiv - Cancer Biology 2024Quote: Using CellTiter-Glo® Luminescent Cell Viability Assay (Promega), cell viability was assessed according to the manufacture’s’ guidelines ...
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bioRxiv - Cancer Biology 2024Quote: Organoid viability was measured with CellTiter-Glo® 3D assay (Promega) accordingly to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... WST-1 analyses were performed using a microplate reader from Promega, and CLARIOstar Plate Reader for MTT analyses ...
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bioRxiv - Cell Biology 2024Quote: ... Prior to imaging of mRNAs cells were exposed to 200 nM JF646 HaloTag ligand (Promega) for 20 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase-Glo 3/7 Assay System (Promega, Cat. G8091) was used to measure the activity of caspase-3 and caspase-7 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5uL of CellTiterGlo reagent (Promega, Madison, WI, Cat. G7570) was added to each well and plates were incubated at room temperature for 10 mins prior to luminescence readout ...
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bioRxiv - Bioengineering 2024Quote: ... (Promega, Madison, WI) using a heat shock protocol ...
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bioRxiv - Bioengineering 2024Quote: ... or a GloMax Plate Reader (Promega). Percent infection was calculated using background-subtracted signals from wells containing virus only as a 100% infection reference ...
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bioRxiv - Bioengineering 2024Quote: The expression level of CYP3A4 was analyzed by using the P450-GloTM CYP3A4 assay kit (Promega; V9001) and following the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2024Quote: The viability of cells was analyzed by using the CellTiter-Glo® 3D cell viability assay kit (Promega; G9681) following the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The viability of HLOs was assessed with CellTiter-Glo® 3D cell viability assay kit (Promega), and luminescence was measured by using the BioTek® Cytation 5 plate reader ...
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bioRxiv - Biophysics 2024Quote: ... EDTA-free protease inhibitor cocktail (Promega) and DNase ...
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bioRxiv - Biophysics 2024Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT Rnase H-(Promega) for 1 h at 42 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The Glucose-Glo and Lactate-Glo kits (Cat #’s J6021 and J5021, Promega, Madison, WI) were used to measure the apparent glucose and lactate concentrations in media samples according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Single-strand oligonucleotides were purchased from Sigma-Aldrich annealed and cloned into LRG2.1 (addgene, #108098) using the BsmBI endonuclease restriction site and T4_DNA ligase (Promega) for subsequent expression of the sgRNA together with EGFP fluorescent protein ...
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bioRxiv - Cancer Biology 2024Quote: The CellTiter-Glo luminescent cell viability assay (Promega) was used to measure cell viability according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x GoTaq Master mix with ROX as a reference dye (Promega) on a Thermo ABI 7500 fast real-time PCR machine (Applied Biosystem ...
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bioRxiv - Bioengineering 2024Quote: ... incubated in 200 µl of 40-fold dilution of Nano-Glo substrate (Promega) in PBS per lung at room temperature for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Bioengineering 2024Quote: ... the NanoLuc gene (Promega) was cloned into XhoI and XbaI restriction sites in pTNT ...
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bioRxiv - Bioengineering 2024Quote: ... were: β-tubulin (1:1500, TUJ1, Promega, G7121), sox10 (1:300 ...
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: ... or a GloMax Plate Reader (Promega). The concentrations of the samples were determined by extrapolation from a standard curve made with a four-parameter nonlinear regression model in Prism.
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bioRxiv - Molecular Biology 2024Quote: Cytotoxicity was measured by Lactate Dehydrogenase (LDH) release using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Microbiology 2024Quote: PCR was performed using GoTaq Master Mix (Promega) using 5 ng of DNA extracted from RS collected after growth on MHA containing 0 or 32 μg/mL cefiderocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2024Quote: ... HeLa cells were routinely tested negative for Mycoplasma (Promega PK-CA20-700-20).
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bioRxiv - Cancer Biology 2024Quote: ... Forty-eight hours later, cells were treated with Thapsigargin (100nM, 4h) and cells were resuspended in passive lysis buffer (Promega). Firefly and Renilla luciferase activities were measured using a dual-luciferase reporter assay system (#E1960 ...
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bioRxiv - Cancer Biology 2024Quote: ... Firefly and Renilla luciferase activities were measured using a dual-luciferase reporter assay system (#E1960; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The PC-3 cell line was authenticated by Macrogen (Korea) using short tandem repeat (STR) profiling (Powerplex 21 System, Promega). All cell lines tested negative for mycoplasma contamination using the Microsart AMP Mycoplasma Kit (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cells with the Maxwell RSC simplyRNA Cells Kit (Promega) on a Maxwell RSC instrument and mRNA reverse transcribed with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase-Glo 3/7 3D Assay (Promega, #G8981) was added and the mixture incubated per manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega) using lipofectamine 3000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested and luciferase activity of cell lysates was measured using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega) with the ViiA7 Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: cDNA synthesis was carried out using GoScript (Promega) and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega ...
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bioRxiv - Cancer Biology 2024Quote: RNA from mouse tissue and from cell lines was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC instrument according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Reagents from Promega were CytoTox 96® Non-Radioactive Cytotoxicity Assay ...
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bioRxiv - Cell Biology 2024Quote: ... subjected to protein precipitation using Chloroform/Methanol and digested with trypsin (Promega). Desalted and acidified peptides (0.1% TFA ...
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bioRxiv - Cell Biology 2024Quote: ... FuGENE® HD Transfection Reagent (Promega), or Lipofectamine 2000 Transfection Reagent (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was measured for LDH (Lactate dehydrogenase) release using the kit of CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... followed by HRP-conjugated secondary anti-mouse antibody (1:5000, Promega W402B), diluted as indicated ...
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bioRxiv - Cell Biology 2024Quote: ... they were injected with SNAP dye JF549 (GA1110, Promega), dissolved in DMSO (Grimm et al. ...
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bioRxiv - Cell Biology 2024Quote: ... as per manufacturer’s protocol (Promega, Madison, WI), followed by a selection of stably engineered cells in media supplemented with 2 µg/mL puromycin for a period of 2-3 weeks.