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Citations for Promega :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Plant Biology 2024Quote: ... Amplicon concentration was quantified with a ND 1000 UV–vis spectrophotometer (NanoDrop Technologies) and Quantus (Promega, USA).
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Plant Biology 2024Quote: ... HaloTag beads (Promega) were aliquoted with 2x buffer volume ...
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bioRxiv - Plant Biology 2024Quote: Total plant RNA was treated with DNase I (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... mRNAs were translated in wheatgerm extract (Promega) supplemented with 20 mM amino acids (lacking methionine ...
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bioRxiv - Plant Biology 2024Quote: ... RNAsin (Promega) was added to the cleared lysate at 1.7 U/μL ...
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bioRxiv - Plant Biology 2024Quote: ... The second PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega) and the Agencourt AMPure XP Kit (Beckman Coulter) ...
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bioRxiv - Physiology 2024Quote: ... The optical density of the standards and samples was recorded at 450 nm wavelength using a microplate reader (Promega, GloMax 9301). All samples were run in triplicates.
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bioRxiv - Cancer Biology 2024Quote: ... where cell viability/ATP production was determined using CellTiter-Glo (Promega).
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bioRxiv - Pathology 2024Quote: ... Enzymatic digestion was performed using Trypsin (Promega, V528X) at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, US) as previously described (56) ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...
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bioRxiv - Plant Biology 2024Quote: Each 20 µl endpoint PCR reaction contained 10 µl of Gotaq Green Master Mix (2X) (Promega, Madison, WI), 1 µl (5 µM ...
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bioRxiv - Plant Biology 2024Quote: ... Dual luciferase assays (Promega) with four independent replicates were employed as reported previously (Schmidt et al. ...
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bioRxiv - Pathology 2024Quote: ... Cell Titer-Glo® Buffer and Substrate were mixed per the manufacturer’s instructions (Promega, Southampton, UK), and then added 100 µl to each well containing the treated cells ...
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bioRxiv - Pathology 2024Quote: ... (Promega, Madison, WA, USA)] was added and left on ice for 30 min to allow the solution to penetrate the gel ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Pathology 2024Quote: ... was purchased from Promega. IL-6 polyclonal antibody (Cat 21865-1-AP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcribed using the Reverse Transcription System (Promega). Mouse thyroid tissue was homogenized in buffer RLT using TissueLyser II (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... The hDF metabolic activity was measured with the CellTiter 96 ® Aqueous MTS Kit (Promega Corp., Madison, WI, USA). At each timepoint ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA accumulation was assessed by real-time qPCR (GoTaq® qPCR Master Mix, Promega) and expression values were normalized to the expression of plant genes At4g26410 and At3g01150 as previously described as a stable reference genes (54) ...
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bioRxiv - Plant Biology 2024Quote: ... it ligated in a pGEMT-Easy vector (Promega Madison, USA) (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA extraction and DNAse treatment were performed using SV Total RNA Isolation System (Promega) as described by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... f1 and f2 were cloned into the pGEM-T Easy vector (Promega) to generate pGEMT-3’gSMR1-f1 and pGEMT-3’gSMR1-f2 ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Pathology 2024Quote: ... Luminescence was detected following addition of 25 µL luciferase substrate (Steady-GLO, Promega). Dose-response curves were fitted using a four-parameter logistic regression and potencies were defined as the EC50s of these fits ...
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bioRxiv - Genetics 2024Quote: ... A 1:10 ratio (enzyme: protein) of Trypsin (Promega) and LysC (Wako ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Adherent cells were harvested with trypsin/EDTA (Merck or Promega, Madison, WI). For lipid droplet staining with Oil Red O ...
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bioRxiv - Plant Biology 2024Quote: ... 5.5 µL of enzyme solution (275 ng of Trypsin Gold, Promega V5280 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were verified by sequencing cloned into the pGL3-Control vector (Promega) by Gibson Assembly ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 μg of pCMV-VSV-G plasmids using FugeneHD (Promega, E2311) in Opti-MEM (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and was reverse transcribed to first strand cDNA using the Reverse Transcription System (A3500, Promega, Madison, Wisconsin, USA). RT–PCR with the forward primer (5’-TGCTTCGAATGCACTCATCACAAC-3′ ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 10 μL/ml RNasin® Plus Ribonuclease Inhibitor (Promega, Madison, WI, USA). The tissue lysates were then homogenized on ice and 1% of each lysate volume was separated as Input samples ...
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bioRxiv - Genetics 2024Quote: ... using the Dual-Luciferase Reporter Assay System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the plates were equilibrated to room temperature before ATP levels were measured with the CellTiter Glo kit (Promega) as the cell viability read-out ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The Bright-Glo™ Luciferase Assay System (Promega, Madison, WI) was used as cell growth indicator for the H37Rv-Luc strain ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... All BRET measurements were made in buffer solutions containing the substrate furimazine (Promega or ChemShuttle ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CMV-LgBit was made by amplifying LgBit from pBiT1.1-N (Promega) and ligating into pcDNA3.1 (+ ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequencing grade trypsin was purchased from Promega (Madison, WI), and animal-Free Recombinant Human EGF from PeproTech (Cranbury ...
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bioRxiv - Pathology 2024Quote: ... and dNTP Mix (Promega) were added and cDNA synthesis was completed by incubation for 1h at 37°C ...
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bioRxiv - Pathology 2024Quote: ... following the manufacturer’s instructions and using the optional RNAse H digestion and oligodT15 primers (Promega). qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing grade trypsin (Promega) was added at an enzyme substrate ratio of 1:100 and digested at 37°C for 16h ...