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Citations for Promega :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: DNA was extracted using the Maxwell RSC Purefood GMO and Authentication Kit (Promega, Madison, WI) (OSD-249) ...
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bioRxiv - Genomics 2024Quote: ... Proliferation was measured using the CellTiter96 AQueous One Solution Cell Proliferation Assay (Promega, G3582) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... the cells were collected and lysed for the reporter luciferase activities measured by using a dual-luciferase reporter assay system (Promega). All the luciferase activity values were gained against the Renilla luciferase control ...
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bioRxiv - Immunology 2024Quote: ... the cells were collected and lysed for reporter activity using the dual-luciferase reporter assay system (Promega). For the detection of m6A site ...
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bioRxiv - Immunology 2024Quote: ... and 100 of pGL4.70 (Promega, Madison WI), a vector encoding the human Renilla gene ...
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bioRxiv - Immunology 2024Quote: ... cellular lysate was assayed for luciferase and Renilla activities using the Dual-Luciferase Reporter assay system (Promega Madison WI). Luminescence was measured using Glomax 20/20 luminometer (Promega ...
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bioRxiv - Immunology 2024Quote: ... Luminescence was measured using Glomax 20/20 luminometer (Promega, Madison WI). Luciferase activities were normalized with Renilla activities.
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bioRxiv - Microbiology 2024Quote: ... followed by gel purification of the 4725 bp band using a Wizard spin column (Promega). A multiple cloning site linker was prepared by annealing the primers oMJF055 and oMJF056 at 8 µM each in a buffer of 50 mM Tris HCl pH7.5 ...
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bioRxiv - Microbiology 2024Quote: ... cell viability assays were performed using the Cell Titer Glo 3D kit (Promega) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Presence and orientation of the linker was confirmed by colony PCR using GoTaq polymerase (Promega) with primers oMJF042 and oMJF055 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were further purified using Wizard Genomic DNA Purification Kit (Promega) following the protocol for Isolation of Genomic DNA from Gram Positive and Gram-Negative Bacteria starting at step 3 ...
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bioRxiv - Microbiology 2024Quote: ... and pBiT2.1-C[TK/SmBiT] were obtained from Promega. ALTO ...
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bioRxiv - Microbiology 2024Quote: ... Wizard Plus SV Minipreps DNA Purification System kit (Promega) was used following the Centrifugation Protocol under Quick Protocol ...
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bioRxiv - Microbiology 2024Quote: ... 6.5 μL FuGENE HD (Promega) was added to this mixture and allowed to incubate for 15 minutes at RT prior to addition to cells ...
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bioRxiv - Microbiology 2024Quote: ... Cultures showing mOrange expression were subsequently miniprepped using a Wizard miniprep kit (Promega). To remove the mOrange insert and generate and empty cloning vector ...
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bioRxiv - Microbiology 2024Quote: ... anti-LgBiT (1:500, N7100, Promega), anti-MCPyV LT (1:500 ...
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bioRxiv - Immunology 2024Quote: The cytotoxicity of CAR T cells was evaluated using a luciferase assay system (Promega, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The presence of released LDH in the supernatant was assessed using the Cytotox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). The extended protocol can be found in the supplementary materials.
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bioRxiv - Microbiology 2024Quote: ... 50 µL of NanoGlow substrate (Promega) was added to each well before measuring in a luminometer (MicroLumat Plus ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence was measured using Bright-Glo Luciferase Assay System (Promega, Madison, WI, USA) in black 96-well plates using VICTOR® Nivo™ Plate Reader (PerkinElmer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 0.4 µl of Fugene 6 according to the manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 1.5 µl of Fugene HD according to manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Microbiology 2024Quote: ... The amplicon was ligated into the pGEM-T Easy vector (Promega, USA), which was then transformed into E ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... and TdT enzyme (Promega). Labelling reactions were carried out overnight at 37 degrees Celsius and were then terminated by incubating at 70 degrees Celsius for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... cDNA was generated with the ImPromII reverse transcription system (Promega #A3800). Quantitative PCR was performed using PerfeCTa qPCR FasMix II (QuantaBio #95119 ...
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bioRxiv - Immunology 2024Quote: ... 7.5% PEG-8000 (Promega, #V3011), 2 U/µL Maxima H-minus reverse transcriptase ...
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bioRxiv - Immunology 2024Quote: ... and reverse-transcribed by GoScript reverse transcription system (Promega). The qRT-PCR mixture consisted of 1 µL of cDNA template ...
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bioRxiv - Immunology 2024Quote: ... The CellTiter-Glo 2.0 assay (Promega) was then used to quantify ATP as a proxy for cell viability at 24h ...
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bioRxiv - Immunology 2024Quote: ... then lysed with Lysis Buffer (Promega) for 15 min on mechanical horizontal rotator ...
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bioRxiv - Immunology 2024Quote: ... LjISG15 and LjMx gene were constructed on basis of pGL3-Basic vector (Promega). All the primers used for vectors construction were shown in Table S1 ...
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bioRxiv - Immunology 2024Quote: ... and finally luciferase activity measured using a commercial luciferase assay system (Promega). The assays were carried out independently three times and the average results were used for statistical analysis.
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bioRxiv - Immunology 2024Quote: ... 100 μL of Bright-GloTM reagent (CAT#E2610, Promega) that was just warmed to room temperature was then added to each well ...
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bioRxiv - Immunology 2024Quote: ... cells was transfected with 250ng of each of 2 pX330 constructs (containing Cas9 and 1 sgRNA) and 500 ng of the plasmid template with FuGene HD transfection reagent (Promega Corporation, Madison, WI) in the recommended conditions ...
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bioRxiv - Immunology 2024Quote: ... mice were humanely killed by xylazine/ ketamine overdose and lungs inflated with 1 ml of 2% low melting temperature agarose (Promega, US) at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... furimazine (NanoGlo, Promega) was administered via intraperitoneal just before acquiring the image using the IVIS Spectrum analyzer (Caliper ...
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bioRxiv - Immunology 2024Quote: ... and the biophotonic signal quantified (Promega GloMax Multi) after 3min ...
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bioRxiv - Immunology 2024Quote: ... Diluted samples (10 μg) were digested with trypsin enzyme (Promega, Madison, WI, USA) at 37 °C for 18 hours ...
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bioRxiv - Immunology 2024Quote: ... pIFNβ-Luc contains the murine IFNβ promoter ligated into pGL3basic (Promega) upstream of the firefly luciferase coding sequence ...
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bioRxiv - Immunology 2024Quote: ... renilla luciferase under a CMV promoter is commercially available from Promega (Promega). pIFNβ-Luc contains the murine IFNβ promoter ligated into pGL3basic (Promega ...
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bioRxiv - Immunology 2024Quote: ... The transfected cells were washed with PBS and lysed with 100μl passive lysis buffer (Promega). The dual luciferase assay kit (Promega ...
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bioRxiv - Immunology 2024Quote: ... A total of 10 ng of cDNA was used for quantitative PCR in a total volume of 10 µl with GoTaq® qPCR Master Mix (Promega) and specific primers ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Immunology 2024Quote: ... RPS18 and UBE2D2 using the corresponding PCR master mix (Promega GmbH, Germany) on a CFX96 Detection System (Bio-Rad Laboratories GmbH ...
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bioRxiv - Immunology 2024Quote: ... and directly used for cDNA synthesis (Promega GmbH, Germany). qRT-PCR was performed for AhR ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from PBMCs using a column-based isolation kit (Promega GmbH, Germany). RNA concentration and purity was measured on a Nanodrop™ microvolume spectralphotometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and 0.1% (wt/vol) APS (Promega, #V3131) and 0.1% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... The dual luciferase assay kit (Promega) was used to determine the luciferase activity ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Immunology 2024Quote: The ADCC activity from sera samples collected at 20 dpi and 14 days post-boost (dpb) was measured using the mFcyRIV ADCC Reporter Bioassay (Promega). MDCK cells were seeded in a 96-well plate at 20,000 cells/well the day before the assay ...