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Citations for Promega :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... f1 and f2 were cloned into the pGEM-T Easy vector (Promega) to generate pGEMT-3’gSMR1-f1 and pGEMT-3’gSMR1-f2 ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Adherent cells were harvested with trypsin/EDTA (Merck or Promega, Madison, WI). For lipid droplet staining with Oil Red O ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were verified by sequencing cloned into the pGL3-Control vector (Promega) by Gibson Assembly ...
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bioRxiv - Neuroscience 2024Quote: ... and was reverse transcribed to first strand cDNA using the Reverse Transcription System (A3500, Promega, Madison, Wisconsin, USA). RT–PCR with the forward primer (5’-TGCTTCGAATGCACTCATCACAAC-3′ ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 10 μL/ml RNasin® Plus Ribonuclease Inhibitor (Promega, Madison, WI, USA). The tissue lysates were then homogenized on ice and 1% of each lysate volume was separated as Input samples ...
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bioRxiv - Genetics 2024Quote: ... using the Dual-Luciferase Reporter Assay System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the plates were equilibrated to room temperature before ATP levels were measured with the CellTiter Glo kit (Promega) as the cell viability read-out ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The Bright-Glo™ Luciferase Assay System (Promega, Madison, WI) was used as cell growth indicator for the H37Rv-Luc strain ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequencing grade trypsin was purchased from Promega (Madison, WI), and animal-Free Recombinant Human EGF from PeproTech (Cranbury ...
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bioRxiv - Pathology 2024Quote: ... and dNTP Mix (Promega) were added and cDNA synthesis was completed by incubation for 1h at 37°C ...
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bioRxiv - Pathology 2024Quote: ... following the manufacturer’s instructions and using the optional RNAse H digestion and oligodT15 primers (Promega). qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing grade trypsin (Promega) was added at an enzyme substrate ratio of 1:100 and digested at 37°C for 16h ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was performed with GoTaq Green Master Mix (Promega; #M7121) following a standard protocol ...
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bioRxiv - Pathology 2024Quote: The pGL4.11 Luciferase Reporter Vector (E6661, Promega) was used to clone the nucleotides −2000 to +99 relative to the transcription start site of the human ACTA2 locus ...
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bioRxiv - Neuroscience 2024Quote: ... cells plated on glass-bottomed dishes were treated with Janelia Fluor 646 HaloTag Ligand (Promega, Cat. No. GA1120) at a final labelling concentration of 200nM (in cell culture media) ...
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bioRxiv - Neuroscience 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were diluted with seven volumes of 50 mM ammonium bicarbonate and incubated for 16 h at 37 °C with 400 ng of trypsin (ratio 1:50; “Trypsin Gold”, Promega).
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bioRxiv - Cancer Biology 2024Quote: ... and bioluminescence was detected by GLOMA MULTI detection system (Promega).
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bioRxiv - Cell Biology 2024Quote: ... NanoLuc expression was measured using NanoGlo® Luciferase Assay System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 200 ng of sequencing grade modified trypsin (Promega) was added to each sample ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (70% confluent) were transfected using FuGene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The activity of in vitro synthesized luciferase was assessed using 5 μL of the substrate from the Steady-Glo Luciferase Assay System (Promega, US). The signal was detected on plate reader Victor X5 2030 (Perkin Elmer ...
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bioRxiv - Microbiology 2024Quote: ... coli S30 Extract System for Linear Templates (Promega, USA). The reactions were carried out in 5 μL aliquots containing 2 μL of S30 Premix ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the Caspase-Glo® 8 Assay (G9952, G8091, G8201, all three from Promega), respectively ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus infectivity was determined 48 hours post inoculation for luciferase activity by luciferase assay reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and chymotrypsin (Promega) to generate peptides for LC-MS/MS using a Thermo Scientific Orbitrap Exploris 480 mass spectrometer coupled to an Ultimate 3000 UHPLC (Dionex) ...
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bioRxiv - Immunology 2024Quote: Adherent HEK293T cells were co-transfected with 0.3μg NF-kB luciferase reporter (pcDNA3.1-luc) (Promega), 0.2μg of β-galactosidase plasmid (CMV.β-galactosidase ...
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bioRxiv - Microbiology 2024Quote: ... and proteins were digested with LysC and trypsin (Promega) in two steps with a final enzyme-to-protein ratio of 1:25 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using ImPromII reverse transcriptase system (Promega, Madison, WI) from 5 μl of stool or tissue RNA ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of samples were mixed with 100 µl BacTiter-GloTM reagent (Promega) and incubated for 5 min in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 30 µl of BacTiter-Glo™ 1X (Promega) was added to each well and luminescence was measured by the Varioskan Lux microplate reader (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... using Fugene HD transfection reagent (Promega). Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3 ...
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bioRxiv - Immunology 2024Quote: ... During the experiment tumor growth was monitored weekly by BLI measurement after intraperitoneal (IP) Luciferin (Promega) injection.
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in cell lysates collected 8 hours after transfection (Promega). To correct for transfection efficiency ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1:20 of the extended live cell substrate EndurazineTM (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... Lambda phage DNA (Promega Corporation) was also fragmented and included as a spike-in to all DNA samples at 0.5%w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with M-MLV reverse transcriptase (Promega) and oligo (dT ...
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bioRxiv - Microbiology 2024Quote: ... 30 µl of BacTiter-Glo™ 1X (Promega) was added to each well and luminescence was measured by the Varioskan Lux microplate reader (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... wells were washed with PBS and biofilms evaluated using the MTS metabolic assay (Promega) as per manufacturer recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was measured with the CellTiter (Promega), and the absorbance was read at 490 nm with the plate reader GloMax® Discover instrument ...
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bioRxiv - Cancer Biology 2024Quote: ... using the GloMax® Discover instrument (Promega) according to the manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... or the CellTiter (Promega), respectively ...
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bioRxiv - Microbiology 2024Quote: ... and the RLUs were quantified using the GLOMAX 20/20 luminometer (Promega, Madison, USA).
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bioRxiv - Microbiology 2024Quote: ... or a GloMax 20/20 Luminometer (Promega Corporation).
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bioRxiv - Microbiology 2024Quote: ... Sequencing-grade trypsin (Promega, 1:25 enzyme:protein ratio) diluted in 20 µL digestion buffer (50 mM TEAB ...
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bioRxiv - Microbiology 2024Quote: Glucose concentrations in spent medium were quantified using the Glucose-Glo™ Assay kit (Promega, Walldorf, Germany). Luminescence measurements of diluted medium samples were performed in white 384-well plates (Greiner BioOne ...