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Citations for Promega :
3651 - 3700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... GoTaq polymerase (Promega), T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: The supernatant of treated cells was removed and cells were then lysed in 1x passive lysis buffer (5x passive lysis buffer diluted in deionized water; Promega, #E194A). For measuring metabolic activity ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 μg LysC (WAKO Chemicals) and 0.5 μg trypsin (Promega) at 30 °C overnight at 300 rpm shaking ...
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bioRxiv - Microbiology 2024Quote: ... the CellTiter-Glo luminescent cell viability assay (Promega, #G7571) according to the manufacturer’s instructions was performed and the luminescent was measured by using an Orion II microplate Luminometer (Berthold ...
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bioRxiv - Microbiology 2024Quote: ... using a spectrophotometer and a Glomax Discover microplate reader (Promega) respectively as described in (Derbise et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The well plates were incubated at 30 °C and the optical density at 560 nm was measured using a plate reader (Promega ProMax). We defined a growth inhibition of at least 50 % in comparison to the growth in the DMSO-supplemented medium as the cut-off to determine the MIC.
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bioRxiv - Biophysics 2024Quote: ... The luciferase activities were measured by using a ONE-Glo Luciferase Assay System (Promega, Madison, WI) and an Ensight Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Biophysics 2024Quote: ... in which the interaction between a Gα subunit and a Gβγ subunit was monitored by the NanoBiT system (Promega). Specifically ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were digested to peptides by adding 25 µL of 50 mM ammonium bicarbonate containing 5 µg of trypsin (Promega) and incubating at 37 °C for 18 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular viability was determined using CellTiter-Glo assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The next day cells were treated with compounds and assessed for cell growth 5 days later using CellTiterGlo (Promega).
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bioRxiv - Microbiology 2024Quote: ... and RNase ONE Ribonuclease (Promega) to deplete host and bacterial nucleic acid ...
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bioRxiv - Bioengineering 2024Quote: ... and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... after 100 µL of luciferin solution (Promega) was added well by well by an autosampler ...
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bioRxiv - Bioengineering 2024Quote: ... after 100 µL of luciferin solution (Promega) was added well by well by an autosampler ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of undiluted sample was loaded onto a white 96 well-plate and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Immunology 2024Quote: ... 6 µg of plasmid DNA was then transfected using FuGENE 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Samples were further diluted to 0.5 M GuHCl and an additional 20 ng/µL LysC was added along with 10 ng/µL Trypsin (Sequencing grade modified, Promega #V5111) and incubated at 37°C for 16 h ...
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bioRxiv - Immunology 2024Quote: ... and luciferase levels were quantified using the Promega Luciferase Assay (Promega) on a BioTek Synergy Neo2 microplate reader.
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bioRxiv - Immunology 2024Quote: ... and the activities of luciferase and Renilla luciferase were determined using the Dual-Luciferase Reporter Assay system (Promega). The luminescence was measured using a FilterMAX F5 microplate reader (MOLECULAR DEVICES) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... followed by digestion with the sequencing grade modified trypsin (V5111, Promega) overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA quality and concentration were estimated via PicoGreen (Promega). Genome library preparations took place at the Broad Institute using a Nextera XT Library Preparation Kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: Cellular and mouse serum cholesterol levels were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (Promega). Cells were cultured in 96-well plates and then treated with the compounds at various concentrations ...
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bioRxiv - Microbiology 2024Quote: Proteins were digested for 11 hours at 37°C under agitation at 600 rpm on a ThermoMix (Thermo) using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Immunology 2024Quote: ... potassium salt (Promega, Ref. E1605).
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bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg trypsin (Promega, Fitchburg, USA) was used to digest 50 μg of total solubilized protein from each sample ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed in triplicate using SYBR Green Master Mix (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed and luciferase activity was measured using the DualLuciferase reporter assay system (Promega) on a Cytation 5 Multi-Mode Reader (BioTek ...
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bioRxiv - Microbiology 2024Quote: ... CellTiter-Glo® 2.0 Cell Viability Assay (Promega) was used to investigate the cytotoxicity of AEVs on THP1-Blue cells ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed and subjected to luciferase assays (Promega). Results were expressed as NLRs calculated as follows ...
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bioRxiv - Microbiology 2024Quote: Cells or tissues were lysed with Passive Lysis Buffer (Promega, USA) before centrifugation for 10 minutes at 13,000g followed by a ten times dilution in Phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were subsequently digested with trypsin (sequencing grade, Promega, Madison, WI), at an enzyme/substrate ratio of 1:50 ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... luminescence activity was analyzed by the Nano-Glo® HiBiT Lytic Detection System (Promega). Based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... After dehydration enough trypsin (6ng/ul, Promega Sequencing grade) was added to just cover the gel pieces and the digestion was allowed to proceed overnight (∼16 hrs. ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s instructions and cDNA was generated by MMLV reverse transcription (Promega, Germany). Quantitative real-time PCR assays were carried out by using GoTaq® qPCR master mix (Promega ...
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bioRxiv - Immunology 2024Quote: ... coli strain BL21(DE3)pLysS (Promega).
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bioRxiv - Microbiology 2024Quote: ... and reverse transcription (RT) was performed using the M-MLV Reverse Transcriptase (Promega). The genomic DNA of mouse tissue was extracted using DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... Transposition was performed directly on nuclei using 25µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina and Digitonin #G9441 from Promega). After pelleting the nuclei by centrifuging at 500g for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with DNAse (RQ1 Rnasa-Free Dnase, Promega, Madison, WS, USA) and retrotranscribed with FireScript KIT Reverse Transcriptase (Solis BioDyne ...
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bioRxiv - Cell Biology 2024Quote: TUNEL staining was performed as previously described using the DeadEnd kit (Promega, Madison, WI, USA, Cat # G3250) (Wubben et al. ...
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bioRxiv - Immunology 2024Quote: ... and RNasin® Ribonuclease Inhibitor (#N2615, Promega; 1:1000)) ...
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bioRxiv - Microbiology 2024Quote: ... (Promega) 0.4 mM dNTP Mix (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 mM dNTP Mix (Promega), 4 μM of each primer (final concentrations) ...