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Citations for Promega :
3501 - 3550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... FuGENETM HD transfection reagent (E2311, Promega); lipofectamine 3000 (L3000001 ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxic activity of the CAR T cells was evaluated with the Promega ONE-Glo™Luciferase Assay (Promega Corporation; cat# E6120) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability results (CellTiter-Fluor, Promega), and luciferase expression data (ONE-Glo Luciferase Assay ...
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bioRxiv - Bioengineering 2024Quote: ... and luciferase expression data (ONE-Glo Luciferase Assay, Promega) were collected 24 h post-treatment using a microplate reader ...
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bioRxiv - Biochemistry 2024Quote: ... immune precipitated samples were washed 5 times with lysis buffer and treated with Trypsin Gold (2 μg/ml; Promega) in 20 mM Tris-HCl (pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Pierce C18 Spin columns were obtained from Thermo Scientific (Pierce Biotechnology ...
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bioRxiv - Biochemistry 2024Quote: Full-length Cdc20 (residues 1-499) with a C-terminal HiBiT tag (GSVSGWRLFKKISGS, Promega) was cloned into a pcDNA3.1(- ...
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bioRxiv - Biochemistry 2024Quote: ... 9 μl translation reaction was mixed with 45 μl luciferase activity reagent (Promega), and the luminescence was measured.
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bioRxiv - Biochemistry 2024Quote: ... 2.5 µg of trypsin (Promega, #V5113) diluted in 20 µL 50 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2024Quote: ... Bovine Fetuin-A (P12763) was purchased from Promega (V4961). The plasmids for His-tagged SmE ...
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bioRxiv - Biochemistry 2024Quote: ... Gel was then placed into a gel drying apparatus (Promega) and allowed to sit at room temperature for 12-16 h with a fan on the apparatus ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 10 µL of 0.5mg/mL trypsin (Promega, V511C) for 6 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Tris-Base (Promega, USA) and other reagents.
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected 24 h post-transfection and lysate was collected and used for luciferase activity assay using the Dual-Luciferase Reporter Assay System (Promega). Data are represented as mean ± standard deviations (SD ...
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bioRxiv - Bioengineering 2024Quote: Cell viability of the inks screening assays and the bioprinted samples was assessed using the 96®AQueous One Solution Cell Proliferation assay (MTS assay, Promega, USA). The supplier protocol was used ...
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bioRxiv - Biochemistry 2024Quote: ... HaloTag-TMR ligand (Promega), HaloTag-biotin ligand (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... anti-HaloTag® (Promega, G9211) and secondary antibodies were HRP-linked anti-rabbit (7074S ...
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bioRxiv - Biochemistry 2024Quote: ... also used Trypsin Golden (Promega, USA), Dithiothreitol DTT (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Tris-Base (Promega, USA) and other reagents.
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bioRxiv - Biochemistry 2024Quote: NAD/NADH-Glo Assay (Promega catalog #G9071) was used to measure NAD+/NADH ratios in WT and GndA mutant cell lines for growth at 30 °C and heat shock ...
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bioRxiv - Biochemistry 2024Quote: BacTiter-Glo Microbial Cell Viability Assay (Promega catalog #G8230) was used to compare total cellular ATP in WT and GndA mutant lines between growth at 37 °C and heat shock conditions ...
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bioRxiv - Biochemistry 2024Quote: ... India. Streptavidin MagneSphere® Paramagnetic Particles (Catalog no. Z5481) were obtained from Promega France ...
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bioRxiv - Biochemistry 2024Quote: ... with on-column RQ1 RNase-Free DNase 1 treatment (Promega, USA). cDNA was synthesized using the SuperScriptTM IV First-Strand Synthesis System (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 U/µL RNasin Plus (Promega, #N261A) with 1 µM protein ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... in 20 mM Tris-HCl (pH7.5) containing 0.04% ProteaseMAX Surfactant (Promega) at 40°C for 2 hrs with shaking ...
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bioRxiv - Biochemistry 2024Quote: ... The final reduced and alkylated flow through from the column was mixed with 1 mg/mL solution of trypsin enzyme (Promega, V511A) in ratio 1:10 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... For digestion three different Trypsin enzymes (Trypsin-Promega; Trypsin/LysC-Promega and fastTrypsin-Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... For digestion three different Trypsin enzymes (Trypsin-Promega; Trypsin/LysC-Promega and fastTrypsin-Promega), 3 different time points incubation (24 ...
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... In a pre-warmed solution of Reconstitution Buffer (20mM HEPES, 250mM KCl, 2mM TCEP, pH 7.5) containing 40 U/mL RNAsin (Cat#N2111, Promega, Madison, WI, USA), each Sm protein sub-complex was combined to a final concentration of 8 µM and incubated for 5 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... bacmid DNA containing all subunits was transfected into Sf9 cells using FuGENE® HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Phosphorylated residues were confirmed by LC-MS after in-gel digestion with trypsin (Promega #V511A) according to standard protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then divided in two to obtain tryptic and Glu-C peptides and diluted to final concentration of 0.1 mg/mL with 50 mM NH4HCO3 for Trypsin digestion and with PBS for Glu-C (Promega) digestion ...
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bioRxiv - Biochemistry 2024Quote: ... and digested overnight with a 1:50 ratio of protein:trypsin (Promega, sequencing grade). Detergents were removed through organic-aqueous phase extraction with ethyl acetate ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining cells were resuspended in 1 mL lysis buffer + protease inhibitor cocktail (Promega) and sonicated using a microtip sonicator for 8 minutes total (8 rounds ...
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellin proteins at a concentration of 0.5 µg/µL in 25 mM ammonium bicarbonate were digested with trypsin (Promega, Madison, WI, USA) using a protein to enzyme ratio of 30:1 (w/w ...
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bioRxiv - Biochemistry 2024Quote: Each biological replicate was split into two 500 uL aliquots and incubated with either 3 µg of trypsin (Promega, sequencing grade) (“surface digest” ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was separated from the cell pellet and centrifuged again with protease inhibitor cocktail (Promega) (6,000 rcf ...
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bioRxiv - Biochemistry 2024Quote: ... Protein cleavage was initiated by the addition 5mM DTT (final concentration) and varying concentrations of ArgC protease (Promega, sequencing grade) with incubation at 37 °C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin digestion was performed with a 1 μg /μL trypsin solution (Trypsin Gold, V528A, Promega) at a ratio of 1:50 at 37 °C for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... Purified Haspin (465-498; 250 nM) was incubated for 1 hour at room temperature following addition of 1 mM ATP (Promega) in a reaction volume of 50 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was isolated with the Wizard Genomic DNA Purification Kit (Promega). KOD Hot Start DNA polymerase (MilliporeSigma ...
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bioRxiv - Zoology 2024Quote: ... and the dsGFP control were synthesized by T7 RiboMAX Express RNAi System (Promega, P1700) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of random hexamers (Promega), and brought to 20 μL total volume with nuclease-free water ...
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bioRxiv - Genetics 2024Quote: ... an additional DNA digestion was performed with RQ1 RNase-Free DNase following the manufacturer’s protocol for RT-PCR (Promega). cDNA synthesis was carried out using the Superscript IV VILO system (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Chemiluminescence was measured every 2.5 min in a GloMax® 96 Microplate Luminometer (Promega).
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bioRxiv - Molecular Biology 2024Quote: NADP(H) content in the yeast cells was assessed with NADP/NADPH-Glo Assay according to the manufacturer’s protocol (Promega) with the modification described by Kwolek-Mirek et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting mixed-population was transfected with 16 μg pcDNA5 TurboID-Mek1 + 1.6 μg pMAX GFP + 66 μL Fugene HD (Promega cat#E2311) + 880 μL DMEM ...