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Citations for Promega :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... MS-grade trypsin (Promega, Madison WI) was added at an enzyme-to-substrate ratio of 1:50 for overnight digestion at 37 ⁰C ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with lysis reagent (Promega) and the cell lysate was collected ...
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bioRxiv - Microbiology 2024Quote: The 3’UTR of human Akt or Pin1 was amplified by PCR from fibroblast genomic DNA and cloned downstream of the Renilla luciferase gene in the psiCHECK-2 dual reporter construct (Promega) by XhoI (Akt ...
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bioRxiv - Microbiology 2024Quote: ... The larvae were immobilized using a 1% low-melting-point agarose (Promega; Cat#: V2111) solution and covered with Volvic water containing 0.2mg/ml tricaine ...
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bioRxiv - Microbiology 2024Quote: ... and 1 U/μL RNasin Plus RNase inhibitor (Promega, Madison, WI, USA) for 1 h at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... gonorrhoeae dedA gene and a kanamycin resistance cassette (Primer: kanR (kanamycin resistance) cassette F/R) were amplified using GoTaq master mix (Promega). The fragments were then assembled using NEBuilder HiFi DNA assembly master mix (NEB ...
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bioRxiv - Microbiology 2024Quote: Northern blot of fungal extracts for MUT4379 was performed as previously described71 using a riboprobe (detecting the positive sense viral RNA) obtained by in vitro transcription of a cDNA fragment cloned into pGEM-T-Easy (PROMEGA, Madison, WI) corresponding to the genomic region between nt 4,800 and nt 4,980 of the deposited fusarivirus sequence (NCBI NC_028470).
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bioRxiv - Microbiology 2024Quote: ... At 48 hours after infection Bright-Glo Luciferase Assay System (Promega, E2610) was used to measure virus neutralization ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-four hours after transfection cells were harvested for luciferase assay using the Dual-Glo Reporter Assay Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by serially diluting virus stocks on 293T cells in the presence of 1 µM oseltamivir and 2.5 µg/ml amphotericin B and measuring luciferase signal 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610), as described previously80.
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bioRxiv - Microbiology 2024Quote: ... PCR products were cloned into pGEM-T Easy Vector (Promega) and standard curves were generated using 10-fold serial dilutions (102 -109 ...
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bioRxiv - Microbiology 2024Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15 min for reverse transcription ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Immunology 2024Quote: ... 1X Gotaq master mix (Promega), and 18 μL of the extracted sample in a final volume of 60 μL ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with phosphate-buffered saline (PBS) and lysed for measuring luciferase activity by the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... SYBR Green Master Mix and Protease inhibitors were purchased from Promega, US ...
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bioRxiv - Immunology 2024Quote: ... an equal volume of One-Glo reagent (Promega, E6120) was added to culture medium for readout using a BMG PHERastar-FSX plate reader ...
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bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
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bioRxiv - Immunology 2024Quote: ... caspase-1 biosensor mice were weighed and injected intraperitoneally with 150 mg/kg VivoGlo Luciferin (Promega). Mice were anesthetized with 2% isoflurane/air mixture and imaged 10 minutes following administration of the luciferase substrate using the IVIS 100 Imaging system (Xenogen) ...
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bioRxiv - Immunology 2024Quote: ... Plates were read two days later by measuring luminescence using Bright-Glo Luciferase Assay System (Promega). Background values were subtracted using the “cells only” condition as background signal ...
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bioRxiv - Immunology 2024Quote: ... Sequencing grade modified trypsin (Promega Corp., Madison, WS) was loaded onto the column (1µg per column ...
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bioRxiv - Immunology 2024Quote: ... pVSVg and pAdVAntage (Promega) using Genejammer (Agilent ...
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bioRxiv - Immunology 2024Quote: ... and M-MLV reverse transcriptase (Promega). Real-time PCR was performed in a QuantStudio 6 Flex PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... The Renilla luciferase internal control vector (pRL-TK) was purchased from Promega. The ubiquitin mutant expression plasmids Lys-48 and Lys-63 (all lysine residues were mutated except Lys-48 or Lys-63 ...
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bioRxiv - Immunology 2024Quote: ... cultures were pulsed with CellTiter 96 Aqueous One Solution Reagent (Promega), 20 μl/well ...
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bioRxiv - Immunology 2024Quote: ... The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with plasmids using FuGENE® HD (Promega). At 72 h post-transfection ...
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bioRxiv - Immunology 2024Quote: ... Quality control for library quantity and size distribution was done using the QuantiFluor dsDNA System (Promega) and High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... the NanoLuciferase activity was measured using the Nano-Glo Luciferase assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... after which the live cells were quantified using the CellTiter-Glo reagent (Promega). The cell titer reagent ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence intensities were measured with AttoPhos Substrate Solution (Promega, Madison, WI) using the Varioskan LUX reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... AMP (200 µM; V506B; Promega) and SAMsite (0,2 µg/µl ...
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bioRxiv - Immunology 2024Quote: ... Fragments were purified by Qiagen cloning standardized protocol and ligated to the pDUAL of interest by the T4 ligase enzyme (Promega) for 1 to 24 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... ATP (200 µM; V915B; Promega), AMP (200 µM ...
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bioRxiv - Immunology 2024Quote: ... and further digestion with trypsin (Promega, 1:50 w/w) at 21 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Then 100 μl of the co-culture was transferred to a white plate with an equal volume of Bright-Glo reagent (Promega, Madison, WI) and was incubated for 5 minutes before luminescence was measured.
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bioRxiv - Immunology 2024Quote: ... cells were lysed in Steady-Glo Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... A T cell’s response to antigen was measured as luciferase activity using the Nano-Glo Luciferase assay system (Promega) according to the manufacturer’s instructions (as above) ...
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bioRxiv - Immunology 2024Quote: ... supernatant was removed for immediate analysis using the LumitTM HMGB1 (Human/Mouse) Immunoassay (Promega, #W6110). Luminescent samples were read in opaque white 96-well plates using a CLARIOstar plate reader (BMG Labtech).
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bioRxiv - Immunology 2024Quote: The screening was performed using the kit ADP-Glo kinase Assay (V6930; Promega). Specifically ...
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bioRxiv - Immunology 2024Quote: HEK293T cells (∼6 × 105 cells per 6-well) were transfected with 1-3 μg of the indicated constructs using FuGENE® HD Transfection Reagent (#E2311, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 30 μL of the live cell dye CellTiter Glo® (Promega, Southampton, UK) was added and luminescence counts read using a TopCount NXT (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described 18,19 ...
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bioRxiv - Microbiology 2024Quote: ... NSP5 B to J were cloned in pCI-Neo (Promega) between MluI/NotI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... leaves were sprayed with 1□mM luciferin (Promega, E1603) and 0.02 % (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3X with TBST and incubated with secondary antibody: anti-rabbit IgG HRP conjugate (Promega, catalog no. W4011, 1:20,000), anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG HRP conjugate (Promega, catalog no. W4021, 1:40,000), goat anti-rabbit IgG HRP conjugate (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL Trypsin (Promega), 2 M urea ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were extracted using a standard Qiagen Miniprep kit and genomic DNA (gDNA) was extracted using a standard Wizard DNA Extraction Kit (Promega) and all DNA was quantified using a Qubit ...