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Citations for Promega :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 20 μl per sample resulting from PCR were mixed with Blue/Orange loading buffer loading dye 6x (PROMEGA, G190A) and loaded onto gel ...
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bioRxiv - Cell Biology 2024Quote: ... however bleaching and imaging of fluorescence recovery was performed using S6l nm excitation of the Halo-TMR (G82S2, Promega) ligand coupled to endogenous CTCF-Halo (excitation power ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was transferred to a new tube and digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µl 50 mM ammonium bicarbonate at 37 °C for 5 h ...
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bioRxiv - Cell Biology 2024Quote: ... they were transfected with either control plasmid or Flag-GSK-3α WT human plasmid using Fugene 6 (Promega, #E2693) at a 3:1 ratio (DNA:Fugene 6) ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was cleaned up with Promega Wizard SV gel and PCR Cleanup System (Promega). The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1.5 μL of FuGENE HD (Promega, catalog no. E2691), all diluted in Opti-MEM I Reduced-Serum Medium (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were harvested for measurement using the Dual-Luciferase Assay System (Promega, catalog no. E4550), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The transfection mix included 0.5 μg of pGL3-enhancer plasmids with firefly luciferase gene sequences driven by an SV40 promoter (Promega, catalog no. E1761), 0.02 μg of pRL-TK Renilla luciferase (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... 8.62 µl ViaFect (Promega E4981) and 103 µl of Opti-MEM (Gibco 31985062) ...
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bioRxiv - Cell Biology 2024Quote: ... 20µL of CellTiter-96®Aqueous and One Solution MTS assay (Promega; #G3580) was added to each well using a multichannel pipette (Transferpette ...
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bioRxiv - Cell Biology 2024Quote: ... and further digested with trypsin (Promega) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... the Firefly and Renilla Luciferase activity was measured in triplicate for each sample using the Dual-Luciferase Reporter Assay System (Promega, Australia). Luciferase activity was expressed as a ratio of Renilla to Firefly activity (Van Etten et al. ...
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bioRxiv - Cell Biology 2024Quote: ... LDH was measured using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, cat. no. G1780). The protocols for BUN and LDH assay were modified to a 384 well microplate format with no efflux dilution.
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bioRxiv - Cell Biology 2024Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol–chloroform to remove protein components ...
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bioRxiv - Cell Biology 2024Quote: ... the 5′-end of each mRNA was capped using Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3582) reagent was added to each well upon completion of experiment duration ...
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bioRxiv - Cell Biology 2024Quote: ... mRNAs were synthesized using RiboMAXTM Large Scale RNA Production Systems-T7 (Promega, Madison, WI, USA) using the corresponding plasmid as a template as previously described (Yamagata et al. ...
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bioRxiv - Cell Biology 2024Quote: ... transfected U2OS cells were incubated with 200 nM Janelia Fluor 646 HaloTag ligand (Promega, Cat# GA1120) for 30-60 minutes and subsequently washed out with DMEM prior to imaging at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... the GSH/GSSG-Glo Assay (Promega) was conducted according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated overnight with 2000 U/mL of universal Type I IFN (PBL Bioscience) and lysed at 24 h post treatment using Passive Lysis Buffer (Promega). Samples were processed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... the CellTox Green assay (Promega) was performed ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaved with the trypsin/endoproteinase LysC mixture (Promega, V5072) using the paramagnetic beads-based method41 ...
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bioRxiv - Cell Biology 2024Quote: ... The mixture of cell lysate/streptavidin agarose was transferred to a WizardR Minicolumn (Promega) and the resins were washed with buffers in the following order ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 0.8 µg/µL trypsin solution (proteomics grade trypsin, Promega). Peptides were then eluted in 65 µl Digestion buffer (50 mM TEAB pH 8.5) ...
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bioRxiv - Cell Biology 2024Quote: ... using Fugene 6 (Promega) at a ratio of 3 µL per 1 µg DNA ...
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bioRxiv - Cell Biology 2024Quote: ... then transfected with 4 μg pLenti-CMV-PuroDEST-ERK-KTR-mClover DNA plus 12 μL FuGENE 6 (Promega Cat. #E2691) in 800 μL of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Cell Biology 2024Quote: Hap1 cells were grown on coverslips and then incubated with 400 nM Janelia Fluor HaloTag ligand 646 (Promega) for 30 minutes then washed three times with media and incubated in fresh media for 30 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The luciferase activity was detected using Dual-Luciferase® Reporter Assay System (Promega, E1910) by Infinite F200 Fluorescence Microplate Reader (Tecan).
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bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: Phosphorylation of HUB2 by SnRK2s was examined with ADP-Glo Protein Kinase Activity Assay Kit (Promega, V9101) with slight modifications (Kathania et al. ...
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bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
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bioRxiv - Physiology 2024Quote: ... revelation was done by incubation with ECL Western Blotting Substrate (Promega, W1001). Signals were recorded with Amersham IQ800 imager (Cytiva) ...
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bioRxiv - Physiology 2024Quote: CellTiter 96® Aqueous One solution Cell Proliferation Assay was used accordingly to manufacturer instruction (Promega). Briefly ...
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bioRxiv - Physiology 2024Quote: ... the PCR products were ligated into the pGEM-T Easy Vector (Promega, Madison, WI), and the sequences were confirmed by Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by the addition of 30 µL of Bright-Glo Luciferase Assay System (Promega, E2610). Luciferase values were immediately read on a Tecan Infinite M1000 plate reader ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fruticulosa plants hydroponically cultivated under 0 mM or 150 mM NaCl was extracted using the Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 24 hours later cells were transfected with 50ng regulatory element plasmid and 50ng Renilla plasmid (pGL 4.74; Promega, E6921) using 0.3µL Fugene HD (Promega ...
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bioRxiv - Genetics 2024Quote: Regulatory elements were amplified by PCR from human genomic DNA using primers listed in Supplementary Table 10 and cloned into the pGL4.23 Luciferase Reporter Vector (Promega, E8411) linearized with NheI and EcoRV (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Luciferase Assay System (Promega, E1501) and the Betafluor Beta Galactosidase Assay Kit (EMD Millipore ...
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bioRxiv - Developmental Biology 2024Quote: ... or experimental constructs along with ptcΔ136-GL3 or TOP-FLASH luciferase reporter constructs and β-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081). Transfections were performed using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... digested with 80 ng/μL trypsin (Promega) at 47°C for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1:10000 HRP-conjugated Anti-Rabbit IgG (Promega W401B). The SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 U/mL RNasin (Promega Cat.#: N2111), 100 ug/mL cycloheximide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... mass spectrometry grade trypsin (Promega Gold) was added at 1:40 w/w and the digestion were let to proceed at 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression of the NLuc-fusion proteins was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega). After the NLuc signal was confirmed 4-5 hours later ...
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bioRxiv - Developmental Biology 2024Quote: See (60) for detailed LipoGlo methods and reagents (Promega Corp., N1110; (106)) ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 10 U/mL DNase (Promega, #M6101) for 1 hour at 4°C with agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Each RNA sample was reverse-transcribed with the GoScript Reverse Transcription system (A5001, Promega) using Oligo(dT ...
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bioRxiv - Developmental Biology 2024Quote: ... Luciferase intensity was measured by the Luciferase Assay System kit (Cat #, E1500; Promega, Madison, WI) on Promega GloMax Explorer (Cat # ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA template was converted to cDNA using M-MLV-Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...