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Citations for Promega :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... after cellular lysis by glass beads and treated with RQ1 RNase-free DNase I (Promega). RNA integrity and concentration were assessed using a NanoDrop Lite Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... the RNA was reverse transcribed to cDNA using the ImProm-II reverse transcription system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and viability determined by MTS assays (Promega, #G5430). Synergy scores were calculated using https://synergyfinder.fimm.fi/ ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were prepared using the M-MLV Reverse Transcriptase protocol (Promega, M1705) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... one of which was incubated with 50 µg /ml of RNase A (Promega) at 37 °C for 60 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with Lys-C (Fujifilm-Wako) or Lys-C / Trypsin mix (Promega). Then samples were extracted by ethyl acetate ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Injection of 40 µl firefly luciferase substrate (Promega) and measurement of luminescence was performed with a plate reader (Viktor Nivo ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 50 ng/well of pGloSensorTM-22F cAMP sensor plasmid (Promega), 50 ng/well of the 3x HA MC4R plasmid and 50 ng/well pcDNA3.1 or MRAP2-FLAG were added to 25 mL/well OptiMEM® media ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The stimulation was stopped by discarding the media and cell lysis was induced by addition of 1x passive lysis buffer (PLB, Promega) and horizontal shaking for 15 min at room temperature.
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bioRxiv - Immunology 2024Quote: ... Anti-Rabbit IgG HRP (1:20,000 Promega W401B). Western blot quantification was done by using ImageStudioLite.
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from bacterial cultures using the Wizard® HMW DNA Extraction Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... We used GoTaq G2 (Promega) with denaturation 94°C (2 min) ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted from the whole body of each individual (male and female nymphs) using ReliaPrep RNA Tissue Miniprep Systems (Promega, Madison, WI, USA). The quality and quantity of the extracted RNA were evaluated using a NanoVue spectrophotometer (GE Healthcare Bio-Sciences ...
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bioRxiv - Genomics 2024Quote: ... [59] followed by a 1,653 nt firefly luciferase reporter sequence derived from plasmid pGL4.10 (Promega). Similarly ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2024Quote: ... following the manufacturer’s instructions (Promega). The concentration of DNA was determined using Qubit 3.0 Fluorometer (Thermo Fischer Scientific ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: One μl of RNA per sample was reverse-transcribed with Promega GoScript reverse transcription system (Promega #A5001), using random hexamer primers ...
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bioRxiv - Immunology 2024Quote: ... Plates were read on GloMax Multi Detection System (Promega) at wavelengths of 480 nm and 520 nm for excitation and emission ...
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bioRxiv - Immunology 2024Quote: ... Absorbance of wells was measured on a GloMax Multi Detection System (Promega) at wavelength of 450nm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we digested tissues using Proteinase K enzyme and used a Maxwell® Rapid Sample Concentrator Instrument with Maxwell® 16 Tissue DNA Purification Kit (Promega Corporation). We used 34 cycles polymerase chain reaction (PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Enzymatic digestion was performed by adding 2 μg trypsin (Gold, Promega, Madison USA) in TEABC 100 mM and incubating overnight at 30 °C ...
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bioRxiv - Immunology 2024Quote: ... The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH) assay to assess cell death using a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega). Both the supernatants and cell pellets were collected for immunoblotting analyses.
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bioRxiv - Immunology 2024Quote: Cell pellets from cells stimulated with inflammasome activators were used to measure cell viability through checking cellular ATP levels with a CellTiter-Glo luminescent cell viability assay kit (Promega). The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH ...
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bioRxiv - Immunology 2024Quote: ... CellTiter-Glo luminescent cell viability assay kit (G7570) and CytoTox 96 non-radioactive cytotoxicity assay kit (G1780) were purchased from Promega.
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentrations were determined using the QuantiFluor ONE dsDNA assay system (Promega). Single-digest SbfI RADseq libraries were prepared following the protocol of (Etter et al. ...
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bioRxiv - Genomics 2024Quote: ... and MgCl2 (final 5 mM) and 5 units of RQ1 RNase-free DNase I (Promega) were added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA digestion was performed with DNase I (Promega), to remove any DNA contamination ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were performed under standard conditions for High Performance GoTaq® G2 Flexi DNA Polymerase (Promega; M7801), with the addition of 5x combined enhancer solution [35] per reaction ...
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bioRxiv - Genomics 2024Quote: ... 1µl PGL3 Basic Vector (Promega, USA, Cat No: E1751) plasmid was added into all prepared standards ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Developmental Biology 2024Quote: ... the pCS2+empty and pCS2*HySp5 plasmids were incubated using the TNT Quick Coupled Transcription/Translation Systems (Promega L2080) and 1 µl was loaded on 10% SDS-PAGE.
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bioRxiv - Genomics 2024Quote: ... Fluorometry (Quantus, Promega) was used for accurate quantification of the gDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... and transcribed using T7 (Promega, P207E) in the presence of Dioxygen-labelled nucleotides (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed 3x 10 minutes in TBS-T before being incubated for 2 hours with the secondary anti-mouse-HRP- or anti-rabbit HRP antibody (1:5000, Promega anti-mouse, W4021; anti-rabbit W4011). The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104) ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNA was used as a template for PCR reactions using Taq polymerase (Promega, Cat. M8305) and specific primers (Supplementary Table S2) ...
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bioRxiv - Immunology 2024Quote: ... Cells were left to rest over night before luciferase activity was measured by using the Dual-Glo Luciferase Assay System (Promega, USA).
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bioRxiv - Genetics 2024Quote: ... 800 µg of total proteins were diluted with HEPES lysis buffer to a final volume of 1.2 mL and then incubated with 80 µL of 20% magnetic streptavidin beads (Promega, cat. # V7820) overnight at 4°C on a tube rotator.
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bioRxiv - Genetics 2024Quote: ... Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The total RNA of each species was extracted from male and female nymphs (each one individual, whole body) using ISOGEN □ (Nippon Gene) and ReliaPrep RNA Tissue Miniprep Systems (Promega). Total RNA extracted from a male and female nymph was mixed in equal amounts ...
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bioRxiv - Evolutionary Biology 2024Quote: ... satsumensis was extracted from male and female last instar nymphs using the ReliaPrep RNA Tissue Miniprep Systems (Promega). Total RNA was extracted from one individual (head and thorax or abdomen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1X of GoTaq® Flexi DNA Green Buffer (Promega, Cat # M891A), 2 mM of MgCl2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... formosanus was extracted from male and female reproductives using the ReliaPrep RNA Tissue Miniprep Systems (Promega). Total RNA was extracted from one individual (head and thorax ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 nM Janelia Fluor® HaloTag® Ligand (Promega, GA112A) was used ...
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bioRxiv - Genomics 2024Quote: ... were lightly digested with 0.2 μl of DNase-I (RQ1 RNase-Free DNase-I, Cat. # M6101, Promega) for 15 sec at 37◦C ...
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Genomics 2024Quote: ... A frozen piece of each liver (∼ 50 mg) was homogenized in 500 μl ice cold 1x Passive lysis buffer (Promega, cat. #E1910) using a rubber-tipped micro pestle fitting the bottom of a 1.7 ml centrifuge tube ...
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bioRxiv - Genomics 2024Quote: ... Renilla luciferin reagent (25 μl of Stop & Glo Reagent, Promega Dual-Luciferase Reporter Assay System; Promega, Madison, WI, cat. #E1910) was injected into each well through one of the injectors of a CentroXS3 instrument (Berthold Technologies) ...