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Citations for Promega :
3601 - 3650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... pooled PCR fragments > 600 bp in length (300 ng per tube x 2 tubes, each diluted with 100 μl of Promega RQ1 DNase 10x buffer (cat. # M6101)) were lightly digested with 0.2 μl of DNase-I (RQ1 RNase-Free DNase-I ...
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bioRxiv - Genomics 2024Quote: ... Renilla luciferin reagent (25 μl of Stop & Glo Reagent, Promega Dual-Luciferase Reporter Assay System ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Immunology 2024Quote: ... Viability was determined using Cell Titer-Glo® (Promega) and a SpectraMax L or SpectraMax iD3 Microplate Reader to read luminescence and measured as a percentage of live control cells (transfected ...
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bioRxiv - Immunology 2024Quote: ... and sequentially digested on the filter with LysC (Wako) and trypsin (Promega). Peptides were cleaned with StageTip C18 columns prior to MS analysis.53 NanoLC–MS/MS was performed on an EASY-nLC 1000 system (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... along with 6 μM EnduRen™ Live Cell Substrate (Promega). Luminescence was then measured over 12 h using the EnVision Multilabel Reader (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted using the ReliaPrep™ RNA Cell Miniprep System (Z6012, Promega) and 500 ng of RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: We performed each experiment in technical triplicate and followed the Dual-Glo Luciferase Assay System protocol (Promega). We transiently transfected adherent HEK293T cells (ATCC ...
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bioRxiv - Genomics 2024Quote: ... The MTS assay (CellTiter 96 AQueous One solution cell proliferation assay, Promega -Ref G3580) was performed by a medium change adding 100 μL of cell culture medium containing 10% MTS solution to the wells ...
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bioRxiv - Developmental Biology 2024Quote: ... The synthesis of cDNA was achieved by incubation in a 20 μl reaction mixture containing 200 U of MMLV Reverse Transcriptase (Promega) and 500 ng of random primer (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and mixed with 10 μl of NanoGlo luciferase assay reagent (Promega). Relative luminescence units (RLU ...
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bioRxiv - Systems Biology 2024Quote: ... samples were diluted 1:4 with 50 mM Tris–HCl pH 8 and sequencing grade trypsin (Promega) was added at 1:50 ratio ...
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bioRxiv - Systems Biology 2024Quote: PDTOs were dissociated into single cells when prepared for imaging and CellTiter-Glo (CTG; Promega, Madison, WI, G9681) cell viability assays ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 1:100 of trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Systems Biology 2024Quote: Tryptic digest of a human cell lysate (K562, Promega) was dissolved in 500 µl 0.1% formic acid (FA ...
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bioRxiv - Synthetic Biology 2024Quote: ... A Bench Top 1-kb DNA Ladder (Promega) was used to estimate the size of DNA.
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bioRxiv - Synthetic Biology 2024Quote: ... NanoGlo assay reagent was obtained from Promega (#N1110). Restriction digestion clean-up and plasmid isolation was performed using Thermofisher GeneJet PCR clean-up and miniprep kits (#K0701 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the final A600 of the culture was measured and 20 μl of culture was diluted with 80μl of fresh LB and added to 100 μl of NanoGlo luciferase assay (Promega) consisting of NanoGlo lysis buffer supplemented with the provided furimazine substrate at a v/v ratio of 50:1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ATP secretion was determined using the CellTiter-Glo® 2.0 (Promega) ATP kit ...
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bioRxiv - Plant Biology 2024Quote: ... methylase was amplified using M.SssI_HindIII forward and reverse primers (Sup Table 6) cloned into pGEMT-Easy (Promega, USA) and sequenced for verification ...
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bioRxiv - Plant Biology 2024Quote: ... using a luciferase assay system (Promega).
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification of WLV was done using PuReTag Ready-to-Go PCR beads (Illustra) or Go Taq DNA polymerase (Promega) according to the manufacturers’ instructions and WLV-specific primer pairs 4194+4195 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the media was discarded and Glosensor substrate reagent (Promega, Madison, WI) was diluted to 1% in 20 mM HEPES in 1X Hank’s balanced salt solution (HBSS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 10 µL of a 1:250 dilution of furimazine (Promega) in stimulation buffer was added ...
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bioRxiv - Physiology 2024Quote: Glucose uptake into pancreatic islets was assessed using the Glucose Uptake-Glo™ assay kit (ref: J1342, Promega). Groups of 5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1µL undiluted Nano-Glo luciferase assay substrate (Promega, N113A) was dispensed into each well and quickly mixed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell viability was assessed by adding an equal volume of Titer-Glo reagent (Promega) to the medium and incubating for 3 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL 1:25 diluted Nano-Glo luciferase assay substrate (Promega, N113A) in PBS was dispensed into each well and quickly mixed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the BTK-nLuc template was purchased from Promega (Cat. No. N2441). The gene coding for BTK kinase domain (residue 382-659 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The trophocidal activity of cyclic peptides was assessed using the CellTiter-Glo 2.0 (Promega, Madison, WI) luminescent viability assay as previously described.9,11 In brief ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20U RNAsin (#N2511; all from Promega, Madison, WI) and RNAse free water ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Plant Biology 2024Quote: ... Extraction of the DNA was carried out using Wizard® Genomic DNA Purification Kit (Promega, catalogue # A1125) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Physiology 2024Quote: ... The resulting cDNA product was inserted into the pCI-neo vector (Promega, Madison, WI, USA) between the EcoRI and XbaI sites ...
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bioRxiv - Physiology 2024Quote: ... Protein (50 μg per sample) was digested overnight at 37°C with LysC (Wako Chemicals) at 1:50 (w/w) ratio and trypsin (Promega, V5113) at 1:100 (w/w ...
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bioRxiv - Physiology 2024Quote: ... HRP Conjugate (1:2,500, Promega, W4011) secondary antibody for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... QuantiFluor® dsDNA System (Promega Corp., Madison, WI), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The 22F-Glosensor plasmid was purchased from Promega, Madison ...
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bioRxiv - Plant Biology 2024Quote: ... The GmPGIP amplicons were purified by the Wizard® SV Gel and PCR Clean-Up System and protocol (Promega). GmPGIP amplicons were ligated directionally into the pENTER/D-TOPO vector and protocol (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR primers were previously described in (Mihaljevic et al., 2016; Popovic et al., 2013) and qPCR was performed using the GoTAQ qPCR mix (Promega, A6001). The elongation factor (EF1α ...
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bioRxiv - Synthetic Biology 2024Quote: ... or PureYield (Promega) or GeneJET (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from pelleted bacterial cells grown in liquid YM medium using the Wizard Genomic DNA Purification System (Promega). Genes of interest were amplified from template DNA by PCR using GOTaq polymerase (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... Chemiluminescence was quantified on a GloMaxⓇ plate reader (luminescence module; Promega, Madison, WI) every 2 min for 30 min with a 1000 ms integration time.
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bioRxiv - Synthetic Biology 2024Quote: ... Successful DNA assembly was verified first by colony PCR using GoTaq® Green Master Mix (Promega) and then with double restriction digestion with EcoRI-HF (NEB ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Glutamate secretion was determined using Glutamate-Glo® Assay (Promega) with samples diluted in Krebs 1:40 prior to measurement ...
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bioRxiv - Plant Biology 2024Quote: The amplified DNA fragment was introduced into the KpnI/SacI sites of the AtS1pro:AlSRKb FLAG+AlSCRb transgene using the LigaFast Rapid DNA Ligation System (Promega, Fitchburg, WI, USA). The ligation products were transformed into Escherichia coli DH5a competent cells ...
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bioRxiv - Plant Biology 2024Quote: ... the genomic DNA from Escherichia coli was purified following the Wizard Genomic DNA Purification Kit (Promega) protocol and served as a template for PCR amplification ...
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bioRxiv - Plant Biology 2024Quote: The pF3A WG (Promega) vectors containing the CDS of αKNL2-C ...