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Citations for Promega :
3901 - 3950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: RNA was extracted using Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA). After quality and quantity control ...
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification was performed in an 8 µl reaction mixture comprising GoTaq Green Master Mix (Promega), primers ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP-IRES-BlastR + 3 μg pMDLg/pRRE-Gag & Pol + 1.5 μg pRSV-Rev + 1.5 μg VSV-G envelope + 42 μL Fugene 6 (Promega cat#E2691) + 600 μL serum-free DMEM ...
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bioRxiv - Molecular Biology 2024Quote: Eluted viral DNA was quantified using Quantus Fluorometer with the QuantiFluor ds DNA system kit (Promega). Minimum concentration of DNA that was considered as sufficient was 1 ng/μl and samples with lower dsDNA concentration were excluded ...
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bioRxiv - Molecular Biology 2024Quote: ... each plate of cells was transfected with 17 μg (pCDNA5) Myc-tdMCP-SNAP + 1.7 μg (pMAX) GFP + 70 μL Fugene HD (Promega cat# E2311) + 935 μL serum-free DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... and GSSG levels) was determined in the yeast cells with GSH/GSSG-Glo Assay according to the manufacturer’s protocol (Promega) adapted for yeast cells as was done by Kwolek-Mirek et al ...
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bioRxiv - Molecular Biology 2024Quote: ... each 15 cm plate was transfected with 17 μg pcDNA5 CRaf-IRES-pErk reporter + 1.7 μg pMAX GFP + 70 μL Fugene HD (Promega cat#E2311) + 950 μL serum-free DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP-IRES-BlastR + 3 μg pMDLg/pRRE-Gag & Pol + 1.5 μg pRSV-Rev + 1.5 μg VSV-G envelope + 42 μL Fugene 6 (Promega cat#E2691) + 600 μL serum-free DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... 16.5 μg attB-plasmid library + 3 μg pCAG-NLS-Bxb1 + 2 μg pMAX-GFP + 2150 μL serum-free DMEM + 80 μL FugeneHD (Promega cat# E2311), then incubated at room temperature for 15 min and added dropwise to cells ...
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bioRxiv - Systems Biology 2024Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the Maxwell RSC Purefood Purification Pathogen kit (Promega Corporation, Madison, Wisconsin, US). We screened all samples for the presence of pathogenic Leptospira spp ...
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bioRxiv - Molecular Biology 2024Quote: ... an initial PCR was performed using gDNA (100 ng) with 1x GoTaq Green Master Mix (Promega #M7123), and 0.5 μM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed at 4 °C in RIPA buffer containing protease and phosphatase inhibitors cocktail (Promega/Sigma). Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... reagents from GoTaq® qPCR Mastermix (Promega, A6002) were used ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 0.5 µg Trypsin/LysC (MS grade, Promega) prepared in 25 mM ammonium bicarbonate ...
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bioRxiv - Evolutionary Biology 2024Quote: In vitro transcription was performed using T7 RNA polymerase (Promega RP2075) and DIG RNA Labeling Mix (Roche 11277073910) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and target bands were purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9282).
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bioRxiv - Evolutionary Biology 2024Quote: ... WT or RIPK1 KO HEK293T cells were seeded in a white 96-well plate and transfected with firefly luciferase fused to either the NF-κB response element (pGL4.32, Promega) or the human IFN-beta promoter (IFN-Beta-pGL3 ...
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bioRxiv - Genomics 2024Quote: ... Relative luciferase activity in the cell lysates was measured using a luciferase assay kit (Promega).
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bioRxiv - Neuroscience 2024Quote: ... 100U/ml RNasin Ribonuclease Inhibitors (Promega, N2515), 100µg/ml Cycloheximide) ...
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bioRxiv - Plant Biology 2024Quote: ... combined with the RQ1 RNase-Free DNase Protocol (Promega, Madison, WA, USA) using ∼100 mg ground tissue ...
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bioRxiv - Plant Biology 2024Quote: ... Gel pieces were rehydrated by adding 6 ng/μL trypsin (Promega SequencingGrade – V5113) in 50 mM TEAB ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified and cloned into the pGEMT vector (Promega, Madison, WI, USA). A minimum of 10 clones per PCR product were then sequenced by the Sanger method to characterize the edited alleles.
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bioRxiv - Genetics 2024Quote: ... and cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) for bisulfite sequencing ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was extracted from blood using the Wizard Genomic DNA Purification Kit (Promega) and from ear biopsies or semen using the Gentra Puregene Cell and Tissue Kit (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... using primers 5’-TCCCTTCCTTCAAGGCTACA-3’ and 5’-GTTAGGAGCCAGAGCAGCAC-3’ and the Go-Taq Flexi DNA Polymerase (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 1 mM dithiothreitol and 1U/µl RNasin ribonuclease inhibitor (Promega) in nuclease-free water ...
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bioRxiv - Systems Biology 2024Quote: ... islets were collected and a DNA assay (QuantiFluor® dsDNA System, Promega, Madison, WI, USA) was conducted for data normalization.
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bioRxiv - Systems Biology 2024Quote: ... 12.5 μl GoTaq qPCR Master Mix (Promega), 9.3 μl nuclease-free H2O and 1 μl of each of the corresponding sense and antisense primers (10 pmol/μl) ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by an overnight digestion with trypsin (sequencing grade, Promega Corporation, Madison, WI, USA) at a 1:100 weight ratio at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then 50 uL of 1x Bright-Glo (2x diluted 1:1 in DPBS; Promega) was added to each well and incubated for 1 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then transferred to 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... The activity of firefly luciferase was then quantified using the bright-Glo™ luciferase assay (Promega). To measure RNA replication and late stages ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR was carried out using GoTaq qPCR Master Mix with SYBR Green (Promega). Primers used are provided in table S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was then amplified with the GenomiPhi V2 DNA Amplification Kit (Cytiva Life Sciences) and again quantified using the Quantus Fluorometer and QuantiFluor dsDNA Kit (Promega). Illumina DNA Prep (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100 ng trypsin (MS grade, Promega) was added (1:100 enzyme to protein ratio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified PCR products were ligated into the pGEM-T Easy Vector (Promega A1360) using 5 μl of 2X Rapid Ligation Buffer ...
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bioRxiv - Immunology 2024Quote: ... 1 U/µL RNaseIn Plus RNase Inhibitor (Promega, Madison, WI), 10 U/µL Superscript II (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Measurements were taken every 10 min in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega) with 5s integration time and 2s delay ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... according to the manufacturer’s instructions and DNase (Promega) treated to maximise RNA quality ...
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bioRxiv - Synthetic Biology 2024Quote: ... FuGENE HD transfection reagent (Promega E2312) was used as specified in the user manual to transfect 400 ng of the hypoxia and/or Wnt recording cassettes into the 24-well cultures ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the TdT variant of interest (750 ng) were transiently transfected using FuGENE HD Transfection Reagent (Promega E2312) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: Cellular ATP content was assessed using the CellTiter-Glo luminescent assay (#G7570, Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were digested with 5 µL of 100 mM Ambic pH 8.8 that contained ∼250 ng/µL of Trypsin/rLysC enzyme mix (Promega) (Total amount 1.25 µg ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution, Promega) for 1-4 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.25 units RNase One (Promega) at 30 °C for 1-2 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... pre-ran reaction solutions were incubated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 nM of linear template DNA) and supplemented with 1 µL of BODIPY-Lys-tRNALys (FluoroTect GreenLys, Promega) to incorporate fluorescently labelled lysine residues in the synthesized proteins ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which they were treated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...