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Citations for Promega :
3451 - 3500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 100 µL of the induced cell culture was mixed with 25 µL of NLuc substrate (Promega) consisting of Nano-Glo® Live Cell Substrate and Nano-Glo® LCS Dilution Buffer at a ratio of 1:19 ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of CellTiter-Glo 2.0 Reagent (Promega, G9241) was added to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin Gold (Promega, Fitchburg, WI, USA) was added at the ratio of 1:50 for enzyme digestion ...
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bioRxiv - Biochemistry 2024Quote: Radioactively labelled proteins were synthesized in the presence of [35S]methionine in a standard or in a transcription and translation coupled reticulocyte lysate system (Promega). Mitochondria (final concentration 0.5 mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... ffLuciferase activity in the lysate was determined by a luciferase assay (Promega, E1500) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... passive lysis buffer (Promega, E194A) was added ...
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bioRxiv - Biochemistry 2024Quote: ... ffLuciferase activity was determined from the lysate’s supernatant by a luciferase assay (Promega, E1500) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was digested using sequencing-grade trypsin (Promega, V511) at 1:50 (20 µg trypsin:1 mg protein ...
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bioRxiv - Molecular Biology 2024Quote: ... The pro(ALBA1):ALBA1 gDNA fragment was subcloned into pGEM-T Easy by A-tailing (Promega) prior to BsaI-restriction cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR amplification was carried out with GoTaq qPCR mix (Promega) in a BioRad CFX-384 thermocycler using a 1/200 dilution of corresponding cDNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... Beta-galactosidase activity was measured using ONPG as substrate (Promega). EGFP and dsRed accumulation was measured by western blot (see below ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... renilla and NanoLuc luciferases were measured in a Berthold luminometer using D-luciferin (Promega), collantrazine (Biotium ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s protocol in GloMax Multi-Detection System with Dual Injectors (Promega). For the production of samples for small RNA sequencing ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were transfected using ProFection Mammalian Transfection System (Promega, PAE1200) with targeted LentiCrispr V2 plasmid (Addgene Plasmid #98290) ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from WBC by using Eastep Super Total RNA Extraction Kit (Promega, China) according to the manufacturer’ s instruction ...
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bioRxiv - Microbiology 2024Quote: Cell viability assays were performed with CellTiter-Glo 2.0 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... the cells were infected with SFV-FFLuc (MOI=0.1) and lysed at 48 hpi using 1X Passive Lysis Buffer (PLB; Promega). FFLuc levels were measured using a Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Relative luciferase intensities were determined using a Dual-luciferase reporter assay Kit (Promega), according to standard operating procedures.
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bioRxiv - Immunology 2024Quote: ... Reactions were performed with the GoTaq Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed in Reporter lysis buffer (Promega, Cat: E397A). The samples stored at -80°C were thawed and kept at RsT for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell viability was assessed utilizing CellTiter-Glo® luminescence assay (Promega, #G755A) as per manufacturer’s instructions and measured with the SPARK 10M reader (Tecan) ...
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bioRxiv - Pathology 2024Quote: ... primers are listed in Table S1) using the Green Master Mix (Promega) specific primers.
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bioRxiv - Immunology 2024Quote: ... PCR was done on gDNA from at least 6 individual larvae using GoTaq Green Master Mix (Promega) (Supp Fig 1A ...
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bioRxiv - Immunology 2024Quote: ... To identify both light chains we performed subcloning of the light chain PCR products into pGEM-T-Vector System (Promega, A3600). First ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µL of DNA was amplified in a 25 µL mixture containing 12.5 µL of GoTaq® Green Master Mix 2X (Promega, Madison, Wisconsin, United States), 2.5 μL of each primer at 10 μM DFA (5’-GAYTTYGCNAGYYTNTAYCC-3’ ...
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bioRxiv - Systems Biology 2024Quote: ... cerevisiae protein lysate (V7461, Promega) to generate three samples ...
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bioRxiv - Bioengineering 2024Quote: ... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (25 µg per sample) was digested overnight at 37 °C with trypsin endoproteinase (Trypsin Gold, Promega), and the digested peptides were desalted using 1 cc C18 cartridges (Waters) ...
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bioRxiv - Bioengineering 2024Quote: CYP3A4 activity was measured using the P450-GloTM CYP3A4 Assay kit (Promega, #V9001) as per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 kbp DNA handles were produced using PCR from λ-DNA (Promega, USA) with a primer carrying either a 5’ biotin or 5’ triple-digoxigenin modification for specific binding to the beads (primers listed in Table S3A ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were plated in black 96-well plates (Grenier Bio One) and furimazine (Promega, Nano-Glo Luciferase Assay System) was added to each well ...
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bioRxiv - Bioengineering 2024Quote: ... Live Cell Substrate (Promega, catalog no. N2011) was prepared according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... urea was diluted to 1.0 M and incubated with AspN (Promega) overnight at 30°C ...
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bioRxiv - Biochemistry 2024Quote: Trypsin Golden (Promega, USA), dithiothreitol DTT (Sigma ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Nano-Glo Live Cell Assay System kit was purchased from Promega (Beijing) Biotech Co. ...
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bioRxiv - Bioengineering 2024Quote: ... FuGENETM HD transfection reagent (E2311, Promega); lipofectamine 3000 (L3000001 ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxic activity of the CAR T cells was evaluated with the Promega ONE-Glo™Luciferase Assay (Promega Corporation; cat# E6120) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability results (CellTiter-Fluor, Promega), and luciferase expression data (ONE-Glo Luciferase Assay ...
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bioRxiv - Bioengineering 2024Quote: ... and luciferase expression data (ONE-Glo Luciferase Assay, Promega) were collected 24 h post-treatment using a microplate reader ...
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bioRxiv - Biochemistry 2024Quote: ... immune precipitated samples were washed 5 times with lysis buffer and treated with Trypsin Gold (2 μg/ml; Promega) in 20 mM Tris-HCl (pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Pierce C18 Spin columns were obtained from Thermo Scientific (Pierce Biotechnology ...
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bioRxiv - Biochemistry 2024Quote: Full-length Cdc20 (residues 1-499) with a C-terminal HiBiT tag (GSVSGWRLFKKISGS, Promega) was cloned into a pcDNA3.1(- ...
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bioRxiv - Biochemistry 2024Quote: ... 9 μl translation reaction was mixed with 45 μl luciferase activity reagent (Promega), and the luminescence was measured.
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then divided in two to obtain tryptic and Glu-C peptides and diluted to final concentration of 0.1 mg/mL with 50 mM NH4HCO3 for Trypsin digestion and with PBS for Glu-C (Promega) digestion ...
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bioRxiv - Biochemistry 2024Quote: ... and digested overnight with a 1:50 ratio of protein:trypsin (Promega, sequencing grade). Detergents were removed through organic-aqueous phase extraction with ethyl acetate ...