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Citations for Promega :
3751 - 3800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence was measured with the Nano-Glo Luciferase Assay system kit from Promega (cat. No. N1110) in a Tecan Infinite 200 PRO microplate reader ...
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bioRxiv - Genetics 2024Quote: ... Proteins were reduced and treated with Trypsin Gold (Promega). The peptide abundance of each sample was measured and each sample was labeled with non-isobaric mass tags ...
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bioRxiv - Genetics 2024Quote: ... Each cDNA sample was amplified in quadruplicate in a 25-μL mixture containing 0.75 U GoTaq DNA polymerase (Promega), 200 μM dNTPs ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were diluted to 1 M urea with 50mM ammonium bicarbonate and digested with chymotrypsin (Promega) at 25°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids were isolated by Wizard® SV minipreps by protocol (Promega). GmPGIP gene sequences were confirmed against the original G ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM DTT) optimized for TEV protease cleavage (ProTEV Plus, Promega, V6101). Samples were adjusted to a final volume of 10 mL with 100U TEV protease and incubated at 4°C for overnight ...
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bioRxiv - Immunology 2024Quote: ... BODIPY-FL labeled tRNA-Lys (Promega L5001) were included in initial test reactions at 1:200 v/v scale ...
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bioRxiv - Molecular Biology 2024Quote: Commercially sourced human DNA (#G1471; Promega Corporation) was diluted using nuclease-free water to prepare four sample dilution pools containing 0.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 µg Trypsin/LysC mix (Promega #V5073) for 1h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Trypsin digestion was performed by adding 10μg Trypsin/ LysC mixture (V5071, Promega) overnight at 37C ...
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bioRxiv - Systems Biology 2024Quote: ... using the NADPH/NADP+ Glo Assay (Promega) with a modification in manufacturer instruction ...
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bioRxiv - Systems Biology 2024Quote: The measurements were performed using the NADPH/NADP+ Glo Assay (Promega) with a modification in manufacturer instruction ...
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bioRxiv - Plant Biology 2024Quote: ... on GloMax® 20/20 Luminometer (Promega).
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bioRxiv - Microbiology 2024Quote: ... except that RNasin 0.36 U/µL (Promega) was also added ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NanoGlo Live Cell Assay System substrate was then added and donor emission (450/8 nm BP filter) and acceptor emission (600 nm LP filter) were immediately measured on a GloMax Discover plate reader (Promega). BRET binding signal was defined as acceptor/donor ratio ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of each of the 3 HIV-envelope expression plasmids were co-transfected with 8 μg of backbone plasmid pSG3ΔEnv in HEK 293T cells using FuGene 6 transfection reagent (Promega, catalog # E5911). Virus was harvested 48 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... PBMCs viability was confirmed using a CytoTox96 non-radioactive cytotoxicity assay kit (Promega). For cytokine quantification experiments ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 µL GoTaq master mix (Promega, Madison, WI, USA) and 1 µL (10 ng µL−1 ...
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bioRxiv - Microbiology 2024Quote: ... and the luciferase signal was developed using the Nano-Glo luciferase assay system (Promega) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then lysed with 40 µL of Renilla luciferase assay lysis buffer (Promega). Luciferase activity was quantified as previously described.
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bioRxiv - Microbiology 2024Quote: ... The cells were then lysed 7 h later in 20 µl of Renilla luciferase assay lysis buffer (Promega), and luciferase activity was quantified using a Tristar LB 941 luminometer (Berthold Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The luciferase activity was then measured with Firefly luciferase assay kit (Promega) according to the manufacturer recommendations and using a luminometer (Berthold).
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bioRxiv - Genomics 2024Quote: ... 2.5 μl of dNTP (2mM, Promega), 1 μl of each primer (10 μM) ...
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bioRxiv - Genomics 2024Quote: ... or a test enhancer sequence cloned upstream (Promega), the pRL plasmid acts as a transfection control with Renilla luciferase driven by an upstream CMV enhancer and CMV promoter (Promega) ...
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bioRxiv - Genomics 2024Quote: ... and lysed in 65 μL 1X passive lysis buffer (in PBS) for 15 min (Promega). 20 μL lysate was then transferred to an opaque flat-bottomed plate for reading with a luminometer (Veritas) ...
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bioRxiv - Genomics 2024Quote: ... the pRL plasmid acts as a transfection control with Renilla luciferase driven by an upstream CMV enhancer and CMV promoter (Promega). Test enhancers were cloned by either PCR of genomic DNA with primers containing NheI and XhoI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... and 0.3 mg/mL RNase A (Promega, Madison, WI, USA). Next ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted from each lysate using a Maxwell® RSC instrument (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The PCR product was cleaned using a PCR clean-up kit (Promega, A9281). The PCR product was then cloned into a lentiviral vector using Golden Gate cloning (Benchling ...
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bioRxiv - Genomics 2024Quote: ... radicosa plant using 100 mg of lyophilized leaf tissue and a Maxwell RSC Genomic DNA Kit (Promega). Sequencing libraries were constructed using a MGIEasy PCR-Free DNA Library Prep Set (MGI) ...
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bioRxiv - Microbiology 2024Quote: The oxidized glutathione (GSSG) and reduced glutathione (GSH) were individually measured by employing the Glutathione Assay Kit (Promega, USA) as described by Nakagawa et al ...
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bioRxiv - Microbiology 2024Quote: ... The preparation of the protein extract of day-14 worms grown on heat-killed OP50 or MKAK9 and bioluminescence were measured for each treatment group using a multimode reader (GloMax Discoverer Promega, Madison, WI, USA). ATP concentration for each treatment group was measured using a log-log plot of the standard curve and expressed as nmol of ATP concentration per mg protein.
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were then lysed and analyzed for firefly and renilla luciferase activities using a GloMax 96 microplate luminometer (Promega). The function of ANP32 was examined using a polymerase assay by co-transfection of DKO cells with different ANP32 proteins or empty vector (20 ng ...
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bioRxiv - Genomics 2024Quote: ... colonies were scrapped from plate for genomic DNA isolation using genomic DNA isolation kit (Promega). The spliced and retrotransposed Alu-Neo cassette (1kb ...
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bioRxiv - Microbiology 2024Quote: ... which was performed using the Wizard Genomic DNA Purification kit (Promega, Leiden, The Netherlands). In brief ...
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bioRxiv - Microbiology 2024Quote: ... M-MLV Reverse Transcriptase (Promega) was used for reverse transcription according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with the RQ1 DNase (Promega) according to the manufacturer’s instructions to avoid genomic DNA contamination ...
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bioRxiv - Microbiology 2024Quote: ... suspensions were subjected to RNase A treatment (Promega) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA was dissolved in DNA Rehydration Buffer (Promega). Presence and integrity of DNA were confirmed using 1·5% agarose Tris-Acetate-EDTA (TAE ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µl of the lysed samples were added to 25 µl PCR reactions with GoTaq Green mastermix (Promega, Cat. No. M7123). After amplification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The target DNA was amplified in PCR reactions prepared with GoTaq Clear Master Mix (Promega, Cat. No. M7123) using 2 µL of the lysed samples per 25 µL PCR reaction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA was further cleaned using the ProNex® Size-Selective Purification System (Promega, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Cell viability was assessed using the CellTiter-Glo luminescent cell viability assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and the luciferase activity was measured using the Dual-Luciferase reporter assay system (Promega). Measurements were acquired by a BioTek synergy HTX multimode reader (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... 15 μl of methyl thiazolyl tetrazolium (MTT, Promega, Madison, WI, USA.) was added to each well and incubated for another 4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cell viability was measured using the RealTime-Glo™ MT Cell Viability Assay (Promega Cat. #G9713) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Dried pieces of gel were rehydrated for 45 min at 4°C in 30 μL of a solution of 50 mM AB containing trypsin at 10 ng/ μL and 0.01% of Protease Max (PM) Surfactant trypsin enhancer (Promega). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega) as previously described (Casabona et al. ...