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Citations for Promega :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized with ImProm-II™ Reverse Transcription System (Promega). qRT-PCR was performed with a CFX96 Real-Time PCR System (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... In-gel digestion with trypsin (Promega, Mannheim, Germany) was performed essentially as described in 61 ...
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bioRxiv - Plant Biology 2024Quote: ... The DNA fragments were cloned into a pTV00 vector using T4 DNA ligase (Promega) and transformed into Agrobacterium tumefaciens strain GV3101.
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bioRxiv - Plant Biology 2024Quote: ... Luciferase activity in the protoplasts was quantified using the Luciferase Assay System (Promega) and a MicroLumat Plus LB96V microplate luminometer (Berthold Technologies).
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bioRxiv - Bioengineering 2024Quote: ... and purified using the SV Gel and PCR Clean-Up System (Promega Corporation) and were sequenced at the Eurofins genomics.
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bioRxiv - Biochemistry 2024Quote: Escherichia coli HB101 (Promega, Madison, WI, USA) was used for plasmid construction and cultivated in LB medium (Bertani 1951 ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) was digested and incubated at 37°C for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 μg of K562 cell tryptic digest (Promega) were added to 100 μL and 500 μL of 0.02% DMNG containing 0.1% TFA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sequencing-grade modified trypsin (Promega) of 1:100 (w/w ...
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bioRxiv - Immunology 2024Quote: ... 1 µg sequence grade trypsin (Promega) was added overnight at 25°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Luciferase reporter assays were conducted 48 hours later using the Dual-Luciferase Reporter Assay System (Promega; CatNo. E1980). Luciferase activity was measured on a CentroXS LB 960 (Berthold Technologies).
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bioRxiv - Systems Biology 2024Quote: ... and an additional 20 ng/µL LysC and 10 ng/µL trypsin (Promega) were added ...
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bioRxiv - Developmental Biology 2024Quote: 3’ UTR fragments of miRNA target genes (for primer sequences see Table S2) were cloned into the XhoI/NotI sites downstream of the synthetic Renilla luciferase gene of psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of MTS solution (CellTiter 96® Aqueous One solution reagent, Promega) was added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using the CellTiter-Glo assay (Promega). Cells were seeded into 96-well plates 24 hours prior to drug addition ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice in nuclei wash and resuspension buffer consisting of PBS with 0.1% BSA and 0.2 U/μl RNase inhibitor (#N2615, Promega, Madison, WI). Cells were passed through a 50 μm CellTricsTM filter (#NC9491906 ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was determined using CellTiter-Glo (#G9243, Promega) following the manufacturer’s protocol and assayed using a GloMax luminometer (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... using 15 µL of 7 ng/µL of modified porcine trypsin (Promega, Madison, WI, USA) in 25 mM NH4HCO3 ...
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bioRxiv - Cancer Biology 2024Quote: 143B cell proliferation was assessed using the CellTiter 96 AQueous Nonradioactive MTS Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Neuroscience 2024Quote: Split nanoluc fragments LgBit and SmBit (Promega) were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB) ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted using the Maxwell® RSC Blood DNA Kit (Promega) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The IL2 URR was cloned into Xho I and Hind III sites of PGL4 Luc vector (Promega) and then ∼500 bp individual sequence representing IL2-IL21 intergenic CNS at −46 ...
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bioRxiv - Molecular Biology 2024Quote: ... After AvrLm4-7 PCR amplification with external primers (Gautier et al., 2023) the PCR products were cloned into pGEM®-T Easy Vector System (PROMEGA) using standard protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then maintained for an additional 40 h and collected for Dual-Luciferase assays (Promega: E2920). In brief ...
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bioRxiv - Genomics 2024Quote: ... and psPAX2 was used for packaging in 293T cells using FuGENE® HD Transfection Reagent (Promega, cat no. E2311). Lentivirus was concentrated using Lenti-X Concentrator (Takara Bio cat no ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using Wizard® HMW DNA Extraction Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2024Quote: Released caspase-1 enzymatic activity of was quantitated with the Caspase-Glo 1 inflammasome assay (Promega), following instructions from the supplier ...
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bioRxiv - Microbiology 2024Quote: ... Adherent cells were measured with a luminescent probe BacTiter Glo (Promega) and read on a Clariostar Plus plate reader ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s protocol in a GloMax Multi-Dectection System with Dual Injectors (Promega).
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bioRxiv - Microbiology 2024Quote: ... ProNex® Size-Selective Purification System (Promega, cat no. NG2001) was used for bead purification/size selection ...
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bioRxiv - Microbiology 2024Quote: ... together with either 20 ng dsRNA targeting FFLuc (dsFFLuc) or 20 ng dsRNA control (dseGFP) and lysed at 24 hpt in 1X PLB (Promega). To determine FFLuc/RLuc levels ...
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bioRxiv - Microbiology 2024Quote: ... dual luciferase assays were performed using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol in a GloMax Multi-Dectection System with Dual Injectors (Promega).
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bioRxiv - Microbiology 2024Quote: ... digested using DpnI (Promega #R6231), and purified again.
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies diluted in 10% DS in PB1 for 3 days at 4°C followed by four washes over the following day with 0.2% Tween-20 (Promega UK Ltd, H5151) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... 100µL of CellTiter-Glo 3D Reagent (Promega, G9681) was aspirated and dispensed into the open media channel and ECM ports ...
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bioRxiv - Bioengineering 2024Quote: ... “Luciferase Assay Systems” protocol was used to measure expression capacity of either mRNA or pDNA LNPs in vitro (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA were reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega). q-PCR was performed using GoTaq q- PCR Mastermix (Promega ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and extracted the Rv DNA with Wizard Genomic DNA Purification Kit (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The cloned into a pGEM-T easy vector (A137A, Promega, Madison, WI, United States). The amplified products were ligated into the Xho I and Sal I sites of the 3’UTR of the firefly luciferase gene in the pmirGLO Dual-Luciferase miRNA Target Expression vector (E 1330 ...
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bioRxiv - Biochemistry 2024Quote: ... pH = 7.4) supplemented with protease and phosphatase inhibitors (Promega and Sigma Aldrich, respectively). Cells were lysed through a series of passages via syringes (25-gauge needle ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The amplified products were ligated into the Xho I and Sal I sites of the 3’UTR of the firefly luciferase gene in the pmirGLO Dual-Luciferase miRNA Target Expression vector (E 1330, Promega) to generate pmiR-Mecp2 ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested and resuspended in ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Promega and Sigma Aldrich, respectively). Lysates were centrifuged at 16,200 x g for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 µg of trypsin (Promega, #V5113) diluted in 20 µL 50 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin (Promega; V5111) was added at a 1:20 enzyme-to-protein ratio following incubation overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were incubated for 2 h at 37 °C followed by DNase RQ1 (Promega, Madison, WI) treatment (2 Units ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested atop the filters overnight at 37°C with mass spectrometry grade trypsin (Promega) at a ratio of 1:50 enzyme:protein ...
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bioRxiv - Bioengineering 2024Quote: ... Luminescent signals were immediately measured using a Glo Max 20/20 Luminometer (Promega).