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Citations for Promega :
101 - 150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed 48 hours after transfection in 100 μL of lysis buffer (Promega). Aliquots of the cell lysates were used to read luciferase emission using Dual-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl RNasin (Promega #N2611), and 1 μl of SuperScript III enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... FuGENE HD (Promega) was added at 30 µl/mL to form a lipid:DNA complex ...
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bioRxiv - Cell Biology 2024Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 µL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Cell Biology 2024Quote: ... The plates were read within 30 min on a GloMax Discover luminometer (Promega) equipped with 450 nm BP filter (donor ...
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bioRxiv - Cell Biology 2024Quote: ... and the cells were washed with PBS and lysed with passive lysis buffer (Promega) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Relative light units (RLUs) were measured using a luminometer (Promega; GloMax). Triplicate data was analyzed in Prism Graphpad to generate IC50 values.
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bioRxiv - Microbiology 2024Quote: Cells in BALF samples were pelleted and resuspended in 100 μL RBC lysis buffer (Promega) for 1 min ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 µl of Nano-Glo reagent (Promega, N1110, 1:1000) was added in each well using a multichannel ...
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bioRxiv - Cell Biology 2024Quote: ... Nano-Glo assay reagent consisted of a 1:1000 dilution of Nano-Glo substrate (Promega; N1110) in buffer (100mM 2-(N-Morpholino)ethanesulfonic acid hydrate (M8250 ...
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bioRxiv - Microbiology 2024Quote: ... Turbo-treated RNA was then reverse transcribed with AMV RT (Promega) primed with random 9-mer (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... FuGENE 6 (Promega) was used for all transfections according to the supplied protocol ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed using Passive Lysis buffer (Promega Madison, WI, USA). Luciferase activities were measured using a GloMax®-Multi+ Detection System (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... along with Luciferase Assay Reagent II and Stop & Glo® Reagent (Promega), following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...
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bioRxiv - Microbiology 2024Quote: ... UTP and CTP were purchased from Promega (Madison, WI). Total tRNAs from E ...
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bioRxiv - Microbiology 2024Quote: ... using GoTaq Flexi Polymerase (Promega, Madison, WI, USA), while for high fidelity amplification ...
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bioRxiv - Microbiology 2024Quote: ... BAL Lactate dehydrogenase (LDH) levels were measured with a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI). Perfused murine lungs were homogenized in 2 mL of PBS ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated with RNase-free DNase I (Promega, USA) at 37°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... Purified products were used as template for 50 µl PCR reactions containing 1x GoTaq (Promega), 0.3 µM each of oligonucleotides JelAP1 ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting products were checked on agarose gel (1%) and purified using the Wizard SV Gel and PCR Clean-Up System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... digested vectors and insert DNA fragments were ligated using T4 DNA Ligase (Promega, Madison, WI, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... RLUs were measured by a luminescence detector (Promega GloMax2020™) at 12 h intervals ...
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bioRxiv - Microbiology 2024Quote: ... and quantified with a Quantus fluorometer (Promega, Madison, WI, USA). Purified libraries were then combined in equimolar amounts prior to paired-end sequencing (2L×L250 bp ...
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bioRxiv - Cell Biology 2024Quote: ... After PCR pre-amplified cDNA were clean-up using 22% home-made PEG beads at a ratio of 0.7 beads:1 sample before concentration quantification using QuantiFluor® dsDNA System (Promega, #E2671). cDNA was normalised and diluted to 100pg µl-1.
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bioRxiv - Cell Biology 2024Quote: ... pre-amplified cDNA libraries were diluted with addition of 9µL water to each well before quantification using QuantiFluor® dsDNA System (Promega, #E2671) and normalisation and dilution to 100pg ul-1.
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bioRxiv - Microbiology 2024Quote: ... Lactic acid production was assessed for each representative strain after 24 hours incubation in the same conditions as for the growth assessment using the Lactate-Glo Assay (Promega) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: used the Maxwell 16 LEV simplyRNA purification system (Promega). C2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL of 0.2 mg/mL LysC protease (Promega) in 50 mM HEPES (pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... and digestion was allowed to proceed overnight at 37°C using trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated with drugs for 24hrs before dual luciferase assays were performed using the Nano-Glo®Dual-Luciferase® Reporter Assay System (Promega, Cat#: N1650) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were lysed in passive lysis buffer (Promega) and activity measured using a dual-luciferase reporter assay system (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... and activity measured using a dual-luciferase reporter assay system (Promega). All assays were performed in triplicate ...
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bioRxiv - Cell Biology 2024Quote: ... Trypsin Platinum (Promega, VA9000); Protease K (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... LDs isolated from BAT were incubated with trypsin (50 μg/ml, Promega) for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 μl cold cell lysis buffer (20 mM HEPES, pH 7.4, 100 mM KCl, 5 mM, MgCl2, 500U/ml RNasin-Plus (Promega), 1x protease inhibitor cocktail (200X ...
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bioRxiv - Microbiology 2024Quote: ... after which proliferation was measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng of trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the DNA was estimated using Nanodrop and the concentration was measured using the QuantiFluor ONE dsDNA System (Promega).
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bioRxiv - Microbiology 2024Quote: ... diluted 10 x into sterile water and processed with the Nano-Glo® Luciferase Assay System (Promega). The bioluminescence was measured using a Spark multimode microplate reader (Tecan).
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Chondrocytes were incubated in the presence of 100 nM of dexamethasone for 60-min prior to the media change ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Samples were maintained in 35 mm dishes sealed with 40 mm glass cover slips (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin/Lys C was purchased from Promega (Madison, USA). ITO slides were obtained from Delta technologies (Loveland ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (Promega) and grown on LB + 100 μg/ml carbenicillin (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... This was cleaned up using a PCR cleanup kit (Promega, A9282). The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... with GoTaq G2 DNA polymerase (Promega, M7845). The PCR was done using biotinylated primers primer CD21 (GAC-CGAGATAGGGTTGAGTG ...
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bioRxiv - Neuroscience 2024Quote: ... gDNA was extracted from a positive clone using the Wizard Genomic DNA Purification Kit (Promega) and subjected to whole genome sequencing (Novogene ...