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Citations for Promega :
3301 - 3350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Plates were read two days later by measuring luminescence using Bright-Glo Luciferase Assay System (Promega). Background values were subtracted using the “cells only” condition as background signal ...
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bioRxiv - Immunology 2024Quote: ... Sequencing grade modified trypsin (Promega Corp., Madison, WS) was loaded onto the column (1µg per column ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with plasmids using FuGENE® HD (Promega). At 72 h post-transfection ...
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bioRxiv - Immunology 2024Quote: ... Quality control for library quantity and size distribution was done using the QuantiFluor dsDNA System (Promega) and High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
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bioRxiv - Microbiology 2024Quote: ... Then they were diluted again to ¼ and digested overnight at 37 °C with sequencing grade-modified trypsin (Promega) at a ratio of 1:50 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then diluted to ½ using ammonium bicarbonate and digested with LysC (Promega) at a ratio of 1:200 for 4 hours at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... and digested on-bead with trypsin (Promega) in 100 mM HEPES pH 8.5 for 3h ...
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bioRxiv - Microbiology 2024Quote: ... and deoxynucleotide triphosphates (dNTPs) (Promega). NTP concentrations were chosen to match levels reported in macrophage cytoplasm67 and were as follows ...
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bioRxiv - Microbiology 2024Quote: ... were quantified using the BacTiter GloTM Microbial Cell Viability Assay (Promega). S ...
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bioRxiv - Microbiology 2024Quote: ... 20 µL of Promega Bright-Glo reagent (Promega E2650) was dispensed into each well ...
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bioRxiv - Microbiology 2024Quote: ... and an equal volume of CellTiter-Glo Luminescent Cell Viability Assay reagent (Promega G7571) was added ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Microbiology 2024Quote: ... copy of the 16S rRNA gene of Bacillus paralicheniformis and a copy of the 18S rRNA gene of Aspergillus fumigatus were separately cloned using the pGEM®-T Easy Vector System (Promega). Positive clones were selected and subsequently grown overnight ...
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bioRxiv - Microbiology 2024Quote: ... Extraction of gDNA was performed using Wizard genomic DNA Kit (Promega). After quantification by nanodrop ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were performed using a 2x GoTaq PCR master mix (Promega; cat# M7122), a 5’-biotinylated M13For and a 5’-biotinylated M13Rev primers (Supp ...
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bioRxiv - Immunology 2024Quote: ... pVSVg and pAdVAntage (Promega) using Genejammer (Agilent ...
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bioRxiv - Immunology 2024Quote: ... and M-MLV reverse transcriptase (Promega). Real-time PCR was performed in a QuantStudio 6 Flex PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... The Renilla luciferase internal control vector (pRL-TK) was purchased from Promega. The ubiquitin mutant expression plasmids Lys-48 and Lys-63 (all lysine residues were mutated except Lys-48 or Lys-63 ...
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bioRxiv - Immunology 2024Quote: ... cultures were pulsed with CellTiter 96 Aqueous One Solution Reagent (Promega), 20 μl/well ...
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bioRxiv - Immunology 2024Quote: ... The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously ...
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bioRxiv - Immunology 2024Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described 18,19 ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual DNA was removed by 0.05 U/µL RQ1 RNase-Free DNase (Promega, M6101) treatment at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% FBS and substrate Nano-Glo Endurazine Live Cell Substrate (Promega, N2570). Immediately post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 µL of HiBit buffer (LgBit 1:200, Promega, N112A; Furimazine 1:100 Promega, N113A in nuclease-free water) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT containing 0.3 µg trypsin (Promega) per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 µL of HiBit buffer (LgBit 1:200, Promega, N112A ...
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bioRxiv - Molecular Biology 2024Quote: ... was incubated with 100 ng FAK tyrosine kinase (Promega #V1971) 50μM DTT ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase activity was measured using the dual-luciferase reporter assay system (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Bacmid DNA was transfected into Sf9 cells using FuGENE transfection reagent (Promega) and virus was passaged twice in the same cell line before large-scale infection ...
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bioRxiv - Molecular Biology 2024Quote: ... H1299 or HEK293 cells were transfected using Fugene HD (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed in 1x passive lysis buffer (PLB; Promega).
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bioRxiv - Molecular Biology 2024Quote: ... DNA was digested using Exonuclease III (Promega) dissolved in the appropriate buffer according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and first strand cDNA was synthesized using the GoScript Reverse Transcription System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Monarch Plasmid miniprep kit (Promega; Wisconsin, USA), and NucleoSpin Plasmid Transfection-grade (Machrey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: ... Signals were detected with ECL Western Blotting Substrate (Promega, Madison, WI, USA) and visualized with Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested 48 hours after transfection and were assayed for the luciferase activity using the luciferase assay system (Promega) and a luminometer (BMG LABTECH ...
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bioRxiv - Molecular Biology 2024Quote: All assays with the HeLaScribe Nuclear Extract in vitro Transcription System (Promega) were performed according to manufacturer protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell Titer-Glo 2.0 Assay Kit was purchased from Promega (G9242). The mycoplasma contamination detection kit was purchased from InvivoGen (rep-mys-50).
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl H2O (Promega, P1199). Samples were incubated 1 hour at 56°C followed by 16 to 24 hours at 80°C and a holding step at 4°C ...
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bioRxiv - Microbiology 2024Quote: Samples of Pst DC3000 subpopulations were collected from colonies grown on swimming medium using 0.13 x 0.33 inch gel extractors (x-tracta™, Promega) and resuspended in TriZol reagent (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The luminescent was measured a GloMax 96 luminometer (Promega). The fold change in luciferase activity was calculated by luminescence in cells transfected with the same amount of minigenome plasmid and empty pCAGGS as a standard.
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bioRxiv - Microbiology 2024Quote: Northern blot of fungal extracts for MUT4379 was performed as previously described71 using a riboprobe (detecting the positive sense viral RNA) obtained by in vitro transcription of a cDNA fragment cloned into pGEM-T-Easy (PROMEGA, Madison, WI) corresponding to the genomic region between nt 4,800 and nt 4,980 of the deposited fusarivirus sequence (NCBI NC_028470).
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bioRxiv - Microbiology 2024Quote: ... At 48 hours after infection Bright-Glo Luciferase Assay System (Promega, E2610) was used to measure virus neutralization ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-four hours after transfection cells were harvested for luciferase assay using the Dual-Glo Reporter Assay Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by serially diluting virus stocks on 293T cells in the presence of 1 µM oseltamivir and 2.5 µg/ml amphotericin B and measuring luciferase signal 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610), as described previously80.
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bioRxiv - Microbiology 2024Quote: ... The samples were analysed for lactate using Lactate-GloTM Assay Kit (Promega) and Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit (AAT Bioquest ...
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bioRxiv - Microbiology 2024Quote: ... NCCR sequences spanning between the ATG start codons for VP2 and LT/ST were synthesized as dsDNA (IDT) and cloned into pGL4.11 (Promega). NCCR sequences are detailed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed and luciferase/renilla activity was analyzed using Dual-Glo luciferase assay system (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RelA and p50 were in vitro transcribed and translated using TnT SP6 coupled wheat germ extract system (Promega) following manufacturer’s instructions for the non-radioactive reaction ...