Labshake search
Citations for Promega :
3201 - 3250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: The 3’UTR of human Akt or Pin1 was amplified by PCR from fibroblast genomic DNA and cloned downstream of the Renilla luciferase gene in the psiCHECK-2 dual reporter construct (Promega) by XhoI (Akt ...
-
bioRxiv - Microbiology 2024Quote: ... The larvae were immobilized using a 1% low-melting-point agarose (Promega; Cat#: V2111) solution and covered with Volvic water containing 0.2mg/ml tricaine ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 U/μL RNasin Plus RNase inhibitor (Promega, Madison, WI, USA) for 1 h at 30°C ...
-
bioRxiv - Microbiology 2024Quote: ... gonorrhoeae dedA gene and a kanamycin resistance cassette (Primer: kanR (kanamycin resistance) cassette F/R) were amplified using GoTaq master mix (Promega). The fragments were then assembled using NEBuilder HiFi DNA assembly master mix (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were lysed and the firefly luciferase and Renilla activities were measured on a GloMax Luminometer (Promega) using a Dual-Glo Luciferase Assay System (Promega).
-
bioRxiv - Biochemistry 2024Quote: ... Mass Spec Grade (V5072) was purchased from Promega (Madison, Wisconsin, USA). Pierce™ Quantitative Colorimetric Peptide Assay (23275 ...
-
bioRxiv - Biochemistry 2024Quote: ... using a Dual-Glo Luciferase Assay System (Promega).
-
bioRxiv - Microbiology 2024Quote: SAM-dependant N6mA DNA methylation activity of PglX was probed in vitro using an MTase-Glo Methytransferase Assay kit (Promega). The kit allows indirect measurement of SAM-dependent methyltransferase activity via production of the SAH reaction product ...
-
bioRxiv - Immunology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: Modifications to the pGL3-promoter plasmid (Promega, Madison, WT) were made using restriction enzymes and T4 DNA ligase from New England Biolabs (NEB ...
-
bioRxiv - Immunology 2024Quote: ... The luciferin reagent (ONE-Glo luciferase reagent, Promega) was added and live target cells were quantified according to the manufacturer’s protocol with a Victor Nivo® multimode plate reader (Perkin Elmer) ...
-
bioRxiv - Immunology 2024Quote: Luciferase assays were performed using the dual luciferase Reporter Assay System (Promega). The pGL3-promoter-EiH plasmid served as positive control ...
-
bioRxiv - Immunology 2024Quote: ... and lysed using the Dual luciferase assay Kit (Promega). Luciferase activity was normalized to the Renilla luciferase activity and expressed as fold-induction relative to the basal activity of non-stimulated cells ...
-
bioRxiv - Immunology 2024Quote: ... and samples were digested overnight with a 1:100 (w/w) Trypsin/Lys-C Mix (Promega) at 37 °C ...
-
bioRxiv - Immunology 2024Quote: ... while pGL3-basic and pGL3-promoter-EiH-R1 were negative controls in different experiments (Promega). For each transfection ...
-
bioRxiv - Immunology 2024Quote: ... after which the live cells were quantified using the CellTiter-Glo reagent (Promega). The cell titer reagent ...
-
bioRxiv - Immunology 2024Quote: ... Fluorescence intensities were measured with AttoPhos Substrate Solution (Promega, Madison, WI) using the Varioskan LUX reader (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2024Quote: ... AMP (200 µM; V506B; Promega) and SAMsite (0,2 µg/µl ...
-
bioRxiv - Immunology 2024Quote: ... supernatant was removed for immediate analysis using the LumitTM HMGB1 (Human/Mouse) Immunoassay (Promega, #W6110). Luminescent samples were read in opaque white 96-well plates using a CLARIOstar plate reader (BMG Labtech).
-
bioRxiv - Immunology 2024Quote: The screening was performed using the kit ADP-Glo kinase Assay (V6930; Promega). Specifically ...
-
bioRxiv - Immunology 2024Quote: HEK293T cells (∼6 × 105 cells per 6-well) were transfected with 1-3 μg of the indicated constructs using FuGENE® HD Transfection Reagent (#E2311, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... 30 μL of the live cell dye CellTiter Glo® (Promega, Southampton, UK) was added and luminescence counts read using a TopCount NXT (Perkin Elmer) ...
-
bioRxiv - Microbiology 2024Quote: ... Luminescence signals were detected by a GloMax Discover Microplate Reader (Promega, USA), and polymerase activity was quantified by normalizing Gaussia luciferase activity to Cypridina luciferase activity.
-
bioRxiv - Microbiology 2024Quote: ATP levels were quantified with the BacTiter-GloTM reagent (Promega). The indicated strains were first pre-grown to an OD600 of 0.5-1.0 in 7H9-OADC ...
-
bioRxiv - Microbiology 2024Quote: ... 2 μl of Nano-Glo Luciferase Assay Reagent (Promega) and 18 μl ddH2O were added to induce luminescence development ...
-
bioRxiv - Microbiology 2024Quote: ... and the amount of intracellular ATP was measured using the CellTiter-Glo assay according to the manufacturer’s protocol (Promega, Madison, WI, USA).
-
bioRxiv - Cell Biology 2024Quote: The NAD+/NADH and NADP+/NADPH measurements were conducted using the NAD+/NADH-Glo Assay (Promega, Cat # G9071) and NADP+/NADPH-Glo Assay (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 μL of the UDP-Glo luminescence detection reagent (Promega) were added and let incubate for 1 h at 25 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... The plates were then transferred into a GloMax Discovery plate reader (Promega) configured according to manufacturer’s instructions for luminescence detection ...
-
bioRxiv - Immunology 2024Quote: ... and further digestion with trypsin (Promega, 1:50 w/w) at 21 °C overnight ...
-
bioRxiv - Immunology 2024Quote: ... Then 100 μl of the co-culture was transferred to a white plate with an equal volume of Bright-Glo reagent (Promega, Madison, WI) and was incubated for 5 minutes before luminescence was measured.
-
bioRxiv - Immunology 2024Quote: ... cells were lysed in Steady-Glo Luciferase Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... A T cell’s response to antigen was measured as luciferase activity using the Nano-Glo Luciferase assay system (Promega) according to the manufacturer’s instructions (as above) ...
-
bioRxiv - Immunology 2024Quote: ... the NanoLuciferase activity was measured using the Nano-Glo Luciferase assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Electro-transformation was achieved using plasmids (PureYield™ Plasmid Midiprep System, Promega, United States) and transformed to recipient E ...
-
bioRxiv - Microbiology 2024Quote: ... A 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based viability assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega) was performed as previously described (25).
-
bioRxiv - Microbiology 2024Quote: ... Each 25 μl PCR reaction was prepared using GoTaq G2 Flexi DNA Polymerase (Promega) and contained 0.5 U μl−1 GoTaq G2 Flexi DNA Polymerase ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50ul (50.000 cells) were transferred to a new 1.5ml Eppendorf tube and 0.5ul 1% Digitonin (Promega #G9441) was added by pipetting well few times ...
-
bioRxiv - Immunology 2024Quote: ... together with a pRL-CMV Renilla reporter construct (Promega). After 24 hours ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1 % ProteaseMax (Promega, USA)) ...
-
bioRxiv - Microbiology 2024Quote: ... cells or biofilms were resuspended in 1x PBS (Difco) measurements carried out using BacTiterGlo™Microbial cell viability Assay (Promega) in accordance with manufacturer’s protocol.
-
bioRxiv - Microbiology 2024Quote: ... Enzymatic surface shaving was carried out with 0.05 µg/µL of trypsin (Promega, Madison, WI, USA) for 30 min at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: Proteins were digested for 11 hours at 37°C under agitation at 600 rpm on a ThermoMix (Thermo) using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
-
bioRxiv - Immunology 2024Quote: ... potassium salt (Promega, Ref. E1605).
-
bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
-
bioRxiv - Microbiology 2024Quote: ... using Bright-Glo™ Luciferase Assay System (E2610, Promega) according to the manufacturer’s instruction.
-
bioRxiv - Microbiology 2024Quote: ... either 0.4 μg/mL or 0.05 μg/mL of trypsin (Promega, V5113) was added to the sucrose buffer ...
-
bioRxiv - Immunology 2024Quote: ... 6 µg of plasmid DNA was then transfected using FuGENE 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Samples were further diluted to 0.5 M GuHCl and an additional 20 ng/µL LysC was added along with 10 ng/µL Trypsin (Sequencing grade modified, Promega #V5111) and incubated at 37°C for 16 h ...
-
bioRxiv - Immunology 2024Quote: ... and luciferase levels were quantified using the Promega Luciferase Assay (Promega) on a BioTek Synergy Neo2 microplate reader.