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Citations for Promega :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and the activities of luciferase and Renilla luciferase were determined using the Dual-Luciferase Reporter Assay system (Promega). The luminescence was measured using a FilterMAX F5 microplate reader (MOLECULAR DEVICES) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... followed by digestion with the sequencing grade modified trypsin (V5111, Promega) overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA quality and concentration were estimated via PicoGreen (Promega). Genome library preparations took place at the Broad Institute using a Nextera XT Library Preparation Kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: Cellular and mouse serum cholesterol levels were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (Promega). Cells were cultured in 96-well plates and then treated with the compounds at various concentrations ...
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bioRxiv - Cell Biology 2024Quote: Nanoluciferase activity was measured from 100 μl of supernatants and lysates (Promega, N1110) using a Perkin Elmer Envision.
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bioRxiv - Cell Biology 2024Quote: ... and 0.2% Triton X-100 supplemented with 65 U/mL RNase out (Promega) and 2x protease inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s instructions and cDNA was generated by MMLV reverse transcription (Promega, Germany). Quantitative real-time PCR assays were carried out by using GoTaq® qPCR master mix (Promega ...
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bioRxiv - Immunology 2024Quote: ... coli strain BL21(DE3)pLysS (Promega).
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bioRxiv - Cell Biology 2024Quote: ... and 4 U/mL RQ1 DNase (Promega), samples were incubated at room temperature (25°C ...
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bioRxiv - Cell Biology 2024Quote: ... beads were equilibrated in 50 mM of ammonium bicarbonate with 2 washes followed by on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-rabbit IgG HRP Conjugate (1:10000, Promega, Cat no. W4011) in blocking buffer and developed using the SuperSignal West Femto Maximum Sensitivity Substrate kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed 3 times for 5 minutes in TBST or TBST-XL for mucin-2 and incubated with anti-mouse IgG HRP Conjugate (1:10000, Promega, Cat no. W4021) or anti-rabbit IgG HRP Conjugate (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: Cell viability was measured by measuring ATP production using Cell Titer Glo assay 2.0 (Promega, Cat no. G9241). Cells were seeded at a density of 15 000 cells/cm2 in an opaque 96 well plate (Greiner Bio-one ...
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bioRxiv - Developmental Biology 2024Quote: ... to visualize proteins in Transcend Chemiluminescence Substrate (Promega). The specificity of all MOs was verified by co-injection with the rescue mRNAs (see below).
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bioRxiv - Developmental Biology 2024Quote: ... The luciferase assay was carried out 48 h after transfection with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega N1610) per manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reaction contained 1x master-mix of the GoTaq qPCR kit (Promega). Reactions were carried out on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Firefly and Renilla luciferase activity was measured 24hr post transfection via dual luciferase assay reporter system (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Cell growth was quantified using the ATP-Glo assay (Promega, G7572). For each condition at least 3 replicates were measured ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were expressed using TnT® Quick Coupled Transcription/Translation System (Promega L1170), according to manufacturer’s instructions and stored at -80°C.
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bioRxiv - Cell Biology 2024Quote: Myc-tagged genes were cloned into pTNT vector (Promega L5620). Proteins were expressed using TnT® Quick Coupled Transcription/Translation System (Promega L1170) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µl biotinylated Transcend tRNA (Promega) was included in the reaction mix resulting in biotinylation of lysine residues ...
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bioRxiv - Genomics 2024Quote: ... the concentration was measured with Quantus Quantifluor ONE dsDNA system (Promega, E4870) and the sample volume was increased up to 100 μl with 10mM Tris pH 8.0 ...
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bioRxiv - Genomics 2024Quote: ... 435 ul of water and 75 μl of 10% Triton X-100 (Promega HP142) was added and 15 min (37°C ...
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bioRxiv - Immunology 2024Quote: ... Transposition was performed directly on nuclei using 25µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina and Digitonin #G9441 from Promega). After pelleting the nuclei by centrifuging at 500g for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified fragments were cloned into pGEM-T easy (Promega) and sequenced at the University of Michigan DNA Sequencing Core facility ...
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bioRxiv - Molecular Biology 2024Quote: ... AMV Reverse Transcriptase 5X Reaction Buffer (M515A, Promega), Aprotinin (BP250310 ...
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bioRxiv - Molecular Biology 2024Quote: ... + 0.5 μg of “reporter” plasmid (pJBM560 and derivatives thereof) + 0.25 μg of pCEP/GFP using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 0.5 μg of “driver” plasmid + 0.5 μg of SVA “reporter” plasmid (pAD26) using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were transfected with 3 μL of FuGENE 6 (Promega) and 1 μg pA2 plasmid DNA per well of a six well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were co-transfected with 0.5 μg of “driver” plasmid (pTMO2F3) + 0.5 μg of pAluneoTet using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... A two-stage enzymatic digestion with 2 hr lysyl endopeptidase (FUJIFILM Wako Chemicals U.S.A. Corporation) and o/n trypsin (Promega) was then performed to generate the crosslinked peptides.
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated oligo-dT (Promega Z5261) was used as a positive control ...
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bioRxiv - Molecular Biology 2024Quote: ... To each well 50 µl luciferase substrate coelenterazine-h (Promega) in Opti-MEM was added to obtain a final concentration of 5 µM and luminescence as well as fluorescence were detected immediately afterwards ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Immunology 2024Quote: ... 1X Gotaq master mix (Promega), and 18 μL of the extracted sample in a final volume of 60 μL ...
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bioRxiv - Immunology 2024Quote: ... Fragments were purified by Qiagen cloning standardized protocol and ligated to the pDUAL of interest by the T4 ligase enzyme (Promega) for 1 to 24 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... ATP (200 µM; V915B; Promega), AMP (200 µM ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with phosphate-buffered saline (PBS) and lysed for measuring luciferase activity by the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... SYBR Green Master Mix and Protease inhibitors were purchased from Promega, US ...
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bioRxiv - Immunology 2024Quote: ... an equal volume of One-Glo reagent (Promega, E6120) was added to culture medium for readout using a BMG PHERastar-FSX plate reader ...
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bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was quantified by using the QuantiFluor dsDNA System (Promega). ChIP was confirmed by qPCR using primers specific for known BfmR-bound DNA compared to negative controls.
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bioRxiv - Microbiology 2024Quote: ... 20 uL of Promega Nano-Glo reagent (Promega N1150) was dispensed into each well using a Multidrop Combi ...
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bioRxiv - Immunology 2024Quote: ... and viral titer was determined by measuring luminescence using Bright-Glo Luciferase Assay System (Promega) at 48 hours post-infection.
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bioRxiv - Immunology 2024Quote: ... The resuspended proteins were digested in trypsin/Lys-C mix (1:25 protease: protein ratio; Promega) for 16 hours at 37 °C in a thermomixer ...
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bioRxiv - Immunology 2024Quote: ... MS-grade trypsin (Promega, Madison WI) was added at an enzyme-to-substrate ratio of 1:50 for overnight digestion at 37 ⁰C ...
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bioRxiv - Immunology 2024Quote: ... caspase-1 biosensor mice were weighed and injected intraperitoneally with 150 mg/kg VivoGlo Luciferin (Promega). Mice were anesthetized with 2% isoflurane/air mixture and imaged 10 minutes following administration of the luciferase substrate using the IVIS 100 Imaging system (Xenogen) ...