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Citations for Promega :
751 - 800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 50 ng of genomic DNA were amplified with the GoTaq (Promega) using specific primers (IDT ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Immunology 2024Quote: ... renilla luciferase under a CMV promoter is commercially available from Promega (Promega). pIFNβ-Luc contains the murine IFNβ promoter ligated into pGL3basic (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Contaminating genomic DNA was removed by RQ1 DNase (M6101) treatment according to the manufacturer’s instructions (Promega Corporation, WI USA). RNA concentration was determined using Qubit (Q32855 ...
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bioRxiv - Molecular Biology 2024Quote: ... Dual-Luciferase Reporter Assay System (Promega) was used to measure luciferase activities ...
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bioRxiv - Molecular Biology 2024Quote: ... Firefly luciferase activity was read first on a GloMax Multi Detection System (Promega) followed by the measurement for Renilla luciferase activity ...
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bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected 24-hr post transfection and firefly and renilla luciferase were analyzed with the Dual Luciferase Reporter Assay system (Promega). Normalization was performed using Western blot analysis for protein concentration ...
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bioRxiv - Immunology 2024Quote: The mouse Il12b promoter (−355 to +55) was cloned into the pGL4.10 vector (Promega). Motif mutations were introduced using the GENEART site-directed mutagenesis system (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The promoter activity was measured based on the production of luminescence by using the Dual-Luciferase Reporter Assay system (#E1910, Promega) and the Glomax Multi+ Detection System (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... Percentage survival was measured photometrically using CellTiter-Glo 2.0 (Promega G9242) viability assay in a microplate reader (Beckman Coulter DTX 880 Multimode Detector) ...
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bioRxiv - Immunology 2024Quote: ... Proliferation was measured via CellTiter-Glo 2.0 Assay (Promega) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... or UAS-V5-Ten-m-ΔECD-FLAG constructs using the FuGENE HD transfection Reagent (Promega). After 48 hours ...
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Specific amplicons were prepared using several primer pairs (Table 1) in combination with GoTaq© DNA Polymerase (Promega).
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bioRxiv - Neuroscience 2024Quote: ... treated with DNaseI (Promega), ethanol precipitated ...
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bioRxiv - Neuroscience 2024Quote: The short TMEM106B 3’ UTR and the long TMEM106B 3’ UTR were amplified from human genomic DNA (H1) and then cloned into the pmirGLO Dual-Luciferase Vector (Promega, E1330) using Gibson assembly ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were digested on beads with trypsin (500 ng of Trypsin Platinum, Promega, Madison, WI, USA) in 100 µl of 50 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... VSV-G and Δ8.9 using FuGENE transfection reagent (Promega, E2311). 24 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed with a Gotaq G2 Hot Start Polymerase system (#M7405, Promega). Pyrosequencing was conducted on a Pyromark Q96 Sequencer using matching reagents (#97204 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA conversion was performed using the GoScript™ Reverse Transcriptase kit (#A5001, Promega). Quantitative real-time PCR was conducted on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNAs were collected from a 10-cm dish directly in 400 μl of 1x Reporter lysis buffer (Promega) containing 0.16 U/μl Ribosafe RNase inhibitors (Bioline) ...
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bioRxiv - Microbiology 2024Quote: ... trypsin digestion was performed after diluting urea to <1.2 M and using 0.5 µg trypsin (Promega), and processed peptides were dissolved in 20 µl of buffer A ...
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Microbiology 2024Quote: ... was prepared using analytical grade agarose powder (Promega, V3121). The GeneRuler 1kb Plus DNA Ladder (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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bioRxiv - Neuroscience 2024Quote: ... genotyping PCR was performed using Go Taq Green Master Mix (Promega, Madison, WI, USA) with the following four primer sets ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg of trypsin (Promega, V5280) was added ...
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bioRxiv - Neuroscience 2024Quote: A DPPIV-Glo Protease Assay Kit (Promega, G8350) was used for DPP9 enzyme activity analysis ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Microbiology 2024Quote: ... Trypsin Gold was purchased from Promega (Madison, WI). Hydrophilic–lipophilic balance (HLB ...
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bioRxiv - Molecular Biology 2024Quote: ... By using CellTiter Blue assay kit (Promega, G8081), 20 μL of CellTiter Blue reagent was added into each well ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... and Cd68 were quantified by using GoTaq PCR master mix (Promega, Madison, WI) in OneStep Plus qPCR (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (1000ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript reverse transcriptase kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Molecular Biology 2024Quote: ... Digoxigenin (Dig)-labeled antisense riboprobes were produced using using T7 RNA polymerase by in vitro transcription with RiboMAX Large Scale RNA Production Systems (Promega). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNAsin Plus (Promega) and vortexed ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by triple DNase I (Promega) treatment to eliminate any genomic DNA contamination ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 350 µL of this aqueous phase underwent automated extraction and cleaning using a Tecan Freedom EVO liquid handler with Promega Purefood protocol and reagents (cell lysis buffer, ReliaPrep™ resin, prepared wash buffer, isopropanol, 50% ethanol and Promega elution buffer). The total extracted and washed DNA was eluted in a final volume of 150 µL.
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated using the SV Total RNA isolation system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested in 1x passive lysis buffer (Promega) at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega) and a luminometer ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... After this colorization the plate was read using the Glomax multi microplate reader (Promega) at an absorbance wavelength of 560nm ...
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bioRxiv - Neuroscience 2024Quote: ... The coupling with the Halotag-Alexa488 ligand (Promega, G1002) to Nlg1-Halotag was performed by incubating Nlg1-HaloTag with an excess (5X ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...