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Citations for Promega :
801 - 850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were seeded in 12 wells plates and transfected (Fugene HD, Promega) the next day with the luciferase reporter vectors (100 ng) ...
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bioRxiv - Microbiology 2024Quote: ... luciferase activity in the infected cells was measured with the Luciferase Assay System (Promega) using a Molecular Devices SpectraMax Microplate Reader.
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bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected 24-hr post transfection and firefly and renilla luciferase were analyzed with the Dual Luciferase Reporter Assay system (Promega). Normalization was performed using Western blot analysis for protein concentration ...
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bioRxiv - Immunology 2024Quote: The mouse Il12b promoter (−355 to +55) was cloned into the pGL4.10 vector (Promega). Motif mutations were introduced using the GENEART site-directed mutagenesis system (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The promoter activity was measured based on the production of luminescence by using the Dual-Luciferase Reporter Assay system (#E1910, Promega) and the Glomax Multi+ Detection System (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each gel plug was then digested with Trypsin Gold (Promega) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... Percentage survival was measured photometrically using CellTiter-Glo 2.0 (Promega G9242) viability assay in a microplate reader (Beckman Coulter DTX 880 Multimode Detector) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Immunology 2024Quote: ... renilla luciferase under a CMV promoter is commercially available from Promega (Promega). pIFNβ-Luc contains the murine IFNβ promoter ligated into pGL3basic (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Contaminating genomic DNA was removed by RQ1 DNase (M6101) treatment according to the manufacturer’s instructions (Promega Corporation, WI USA). RNA concentration was determined using Qubit (Q32855 ...
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bioRxiv - Molecular Biology 2024Quote: ... Dual-Luciferase Reporter Assay System (Promega) was used to measure luciferase activities ...
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bioRxiv - Molecular Biology 2024Quote: ... Firefly luciferase activity was read first on a GloMax Multi Detection System (Promega) followed by the measurement for Renilla luciferase activity ...
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bioRxiv - Microbiology 2024Quote: ... on the apical side containing a viability dye (Promega, G8080). After an overnight incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with RQ1 DNAse (Promega). 2 µg total RNA was reverse-transcribed by Superscript III with random hexamers (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cytotoxicity was measured by lactate dehydrogenase (LDH) release using CytoTox96 Non-radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of sequencing grade trypsin (Promega, Wisconsin, USA) was added and samples were incubated at 37 °C overnight.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM dithiothreitol (DTT) and 0.4 µg sequencing grade trypsin (Promega) for 1 h at 25°C with continuous agitation on a shaker operating at 1000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Infectivity was measured either by luciferase using the Luciferase Assay System Kit (Promega) or by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... samples were further purified using the ProNex Size-Selective Purification System (Promega). Concentrations were measured by Qubit dsDNA broad range assay ...
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bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was evaluated by quantification of lactate dehydrogenase (LDH) release in cell cultures using CytoTox 96 nonradioactive cytotoxicity assay (Promega, Charbonnières-les-Bains, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.2 U/µl RNasin plus (Promega) and purified recombinant protein as specified were incubated at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... (Promega, W4011).
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and RNasin Plus RNase Inhibitor (Promega N2611) at 60 U/mL for RNA experiments ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequencing grade trypsin was purchased from Promega (Madison, WI), and animal-Free Recombinant Human EGF from PeproTech (Cranbury ...
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bioRxiv - Pathology 2024Quote: ... and dNTP Mix (Promega) were added and cDNA synthesis was completed by incubation for 1h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl of CellTiter-Blue (Promega) was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with the plasmid using FuGENE HD (Promega, E2311). The indicated experiments were carried out 12–18 h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were processed according to the manufacturer’s recommendations to assess intra-cellular ATP levels as a measure of cellular proliferation and viability using CellTiter-Glo (Promega, Madison, WI). Nonlinear dose-response curves showing the effects of compound concentration on growth inhibition were generated using GraphPad Prism.
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bioRxiv - Cancer Biology 2024Quote: ... and luciferase activity was measured in triplicate using the dual luciferase reporter kit (Promega) and a Glo-Max 96 Multidetector system (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... Following by reverse transcription to cDNA using the GoScript Reverse Transcription System (Promega, A5001). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the GoTaq QPCR Master Mix (Promega, A6001) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with an HRP-conjugated secondary antibody (Promega, Cat# W4021, 1:10,000 dilution) in blocking solution ...
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bioRxiv - Plant Biology 2024Quote: ... 54] and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µl of the RNA from input and supernatant and 7 µl of the pre-eluates and eluates were digested with RQ1 DNase I (Promega, Walldorf, Germany) and cDNA synthesis was carried out with Superscript IV reverse transcriptase (Thermo Fischer ...
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bioRxiv - Neuroscience 2024Quote: ... and the luminescence was quantified by the GloMax Luminometer plate reader (Promega).
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bioRxiv - Neuroscience 2024Quote: The CellTiter-Glo assay (Promega; Madison ...
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bioRxiv - Plant Biology 2024Quote: ... following the instructions of the manufacturer (Promega). Two nanograms of cDNA were used as template in each qRT-PCR ...
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bioRxiv - Physiology 2024Quote: ... 1μg of RNA was loaded into each reaction including Oligo(dT) (Promega), dNTP mix (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were lysed with 0.05% aqueous SDS and luminescence was measured using the GloMax 20/20 Luminometer (Promega) [15].
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bioRxiv - Pathology 2024Quote: A BRE-MLP-Luc plasmid was constructed as described by Korchynskyi & Dijke (46) with the exception that the reporter was inserted into a pGL4.20 vector (Promega). For generating the cell line ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and nanoluc substrate (Promega, 1:200) diluted in 1X passive lysis buffer (Promega) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... diluted in 1X passive lysis buffer (Promega). Luminescence of the fractions was measured with a luminometer (Promega ...