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Citations for Promega :
901 - 950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1522bp using primers 5’-AAGGTACCTGAGGCTGGAGAGATGGCC-3’ and 3’-TAAAAGCTTCACCGGACTGGGCTAGTTCAG-5’ were PCR amplified and cloned in promoterless PGL3 enhancer empty vector (Promega, E1771) at the upstream of luciferase gene ...
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bioRxiv - Microbiology 2024Quote: ... mNeonGreen and Halotag fusion proteins were detected using polyclonal anti-mNeonGreen (NC, unpublished) and anti-Halotag (Promega) antibodies diluted at 1:10,000 and 1:2,000 respectively ...
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bioRxiv - Microbiology 2024Quote: ... or LDH-Glo Cytotoxicity Assay (Promega, J2380) were used to determine cell death ...
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bioRxiv - Microbiology 2024Quote: ... 6μL Promega PCR amplification-grade water (Promega) and 2 μL of the sample DNA (corresponding to about 20 ng) ...
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bioRxiv - Microbiology 2024Quote: ... 4,7 μL of PCR amplification-grade water (Promega Corporation, Wisconsin, USA) and 2 μL of sample DNA (about 20 ng) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µl of 5X Green GoTaq Buffer (Promega, M791A), 0.2 µl of GoTaq DNA polymerase (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... 1–5 µl of gDNA was mixed with 0.5 µl of 10 mM dNTP mix (Promega, C1141, Madison, WI), 10 µl of 25 mM MgCl2 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 µl of GoTaq DNA polymerase (Promega, M3005), 1 µl of each PCR primer stock (100 µM each) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted and transcribed using Maxwell RSC instrument and Maxwell RSC RNA kit (Promega) and quantified by subsequent qPCR using a LightCycler 480 ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were then single cell plated and positive colonies were selected with HaloTag TMR dye (Promega) and verified by PCR of genomic DNA.
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bioRxiv - Neuroscience 2024Quote: ... bIII-tubulin (1:1000, Promega, G7121), LMX1(1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... Each gel plug was then digested with Trypsin Gold (Promega) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... 1U/μL RNAsin Plus (Promega) and 100nM DNA probes ...
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bioRxiv - Immunology 2024Quote: ... permeabilized with 1% TRITON X-100/PBS (Promega) and blocked with 5% FBS/PBS ...
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bioRxiv - Immunology 2024Quote: ... Digitonin (Promega) and Proteinase K (Roche) ...
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bioRxiv - Immunology 2024Quote: ... 75 µL of Bio-Glo luciferase reagent (Promega) was then added to each well and the luminescence was read in a Synergy 4 (BioTek ...
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufactureŕs protocol.
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bioRxiv - Molecular Biology 2024Quote: LDH release was quantified according to the manufacturer’s recommendations (LDH-Glo Cytotoxicity Assay, Promega, Madison, WI). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μL of diluted Nano-Glo reagent (Promega, Walldorf, Germany) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of CellTiter-Blue reagent (Promega) was then added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was then cloned in pGEMT-easy cloning vector (Promega, USA). Cloning and HvHsfA6a gene was confirmed by sequencing followed by BLAST search using NCBI ...
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bioRxiv - Molecular Biology 2024Quote: ... The clear supernatant was incubated overnight at 4°C with 100 μl of prewashed Magne® HaloTag® Beads (Promega, WI, USA). Post-incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... with Halo-Tag TMRDirect ligand (Promega, WI, USA), as per the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were lysed in 300 μl of mammalian cell lysis buffer (Promega, WI, USA), containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... HaloTag® TMRDirect™ Ligand (G2991) was sourced from Promega. AcTEV™ Protease (12575015 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.2 U/µl RNasin plus (Promega) and purified recombinant protein as specified were incubated at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.7U/μL RNAsin Plus (Promega)) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and 40 U RNasinPlus (Promega). The temperature program was chosen as follows ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was isolated using the Promega Wizard gDNA kit (Cat.A1120, Promega) and quantified by QuBit 3.0 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Fifteen microliters of reconstituted One-Glo Ex Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... and pET-Duet-PpNSE6(aa1-370)-StrepTwin-Stag was performed using the TNT kit (Promega, USA). Radiolabeled methionines (Met S35 ...
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bioRxiv - Molecular Biology 2024Quote: ... Powdered plant material (150 mg) was aliquoted and extracted with 1.25 mL hexadecyltrimethylammonium bromide (CTAB) buffer (MC1411, Promega, Madison, WI) containing proteinase K (ref 25530049 ...
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bioRxiv - Molecular Biology 2024Quote: ... Mass Spectrometry grade trypsin (Promega), reconstituted at 10 ng/μL 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with 1 μg/μL of RNA concentration using MMLV-RT enzyme (Promega, USA). HvHsfA6a gene specific and Actin primers designed by using Primer express 3.0 software (Thermoscientific USA ...
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bioRxiv - Molecular Biology 2024Quote: ... stored at 4°C and assayed the following day for lactate dehydrogenase (LDH) activity as a measure of cell death using a Cytotox 96® Non-radioactive Cytotoxicity Assay (Promega, Madison, Wisconsin, USA).
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bioRxiv - Immunology 2024Quote: ... and 1 μg of RNA used as a template for cDNA synthesis with random primers and ImProm-II reverse transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... CellTiter-Glo (Promega, G9241) was added to the media ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing-grade trypsin (Promega, 1:25 enzyme:protein ratio) diluted in 20 µl digestion buffer (50 mM TEAB ...
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bioRxiv - Immunology 2024Quote: ... To promote transfection mixes were supplied by Fugene HD transfection reagent (Promega Madison WI). On the third day ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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bioRxiv - Immunology 2024Quote: ... 40 μL of One-GloEX (Promega) was added to the cells and incubated in the dark for 5 min prior to reading on an Agilent BioTek Neo2 plate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with 2 µg Trypsin (Promega) overnight at 37 °C shaking at 1200 RPM on a thermomixer ...
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bioRxiv - Molecular Biology 2024Quote: Magne® HaloTag® beads (G7281) and SNAP-Capture Magnetic Beads (S9145) were acquired from Promega (Madison, WI) and New England Biolabs (Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit anti-HaloTag® polyclonal antibody (G9281) was obtained from Promega. Mouse anti-tubulin monoclonal antibody (66031–1-Ig ...
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bioRxiv - Molecular Biology 2024Quote: MTS assays were undertaken using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega) or Abcam MTS assay kit (ab197010) ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was executed with the GoTaq qPCR Mastermix (Promega) employing the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then a final solution comprised of 17% formamide + 5% Triethalomine + 70% glycerol + 3% Tris (w/v, Tris, Promega, H5135) made in basic DDH20 ...
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bioRxiv - Molecular Biology 2024Quote: ... the relative luciferase activity was measured according to manufactureŕs instructions (Promega, #E1910). The relative luciferase activity was calculated by the ratio of firefly luciferase activity to internal Renilla luciferase activity.