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Citations for Promega :
951 - 1000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... each WT ZNF410 peak was amplified by PCR from genomic DNA and cloned into pGL4.24 vector (Promega, #E8421), digested by KpnI and XhoI ...
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bioRxiv - Molecular Biology 2024Quote: Each ZNF410 peak and all the variants of ZNF410 motif mutant sequences were sub-cloned into pGL4.24 plasmid (Promega, #E8421). About 1.2 μg WT or constructed pGL4.24 plasmid and 0.2 μg pGL4.74 control plasmid (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... About 1.2 μg WT or constructed pGL4.24 plasmid and 0.2 μg pGL4.74 control plasmid (Promega, #E6921) were co-transfected into K562 cells by Neon™ NxT Electroporation System (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega), 0.125 µCi/µL 35S-methionine (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega) and 0.125 mg/mL of home-made recombinant T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega), 0.125 µCi/µL 35S-methionine (Perkin-Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... cell viability was quantified using the CellTiterGlo Luminescent Cell Viability Assay (Promega) measuring luminescence on a SpectraMax M3 plate reader or BioTek Synergy 2 plate reader ...
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bioRxiv - Genetics 2024Quote: ... dNTP mix (0.3mM each) (Promega)) with 10 cycles of PCR ...
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bioRxiv - Immunology 2024Quote: ... Luminescence was measured on a GloMax Navigator Luminometer (Promega) using Bright-Glo luciferase substrate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short Solexa primers P5 and P3 (Suppl. Tab. 9) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... and primers were removed using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.4 (v/v ...
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bioRxiv - Immunology 2024Quote: The PCR products representing genomic and cDNA sequences were purified using PCR purification kit (Promega) and subsequently sequenced using Sanger sequencing at Micromon Genomics ...
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bioRxiv - Biochemistry 2024Quote: ... Halo-SIN3A was cloned from ORF Clone # FHC11647 (Promega) by first mutating the codon for amino acid 109 from GCT (A ...
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bioRxiv - Biochemistry 2024Quote: ... 1:100 Endurazine Luciferase live cell substrate (Promega) were added to each well ...
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bioRxiv - Biochemistry 2024Quote: ... and digested with mass spectrometry grade trypsin (Promega). The extracted peptides were analysed by LC-MS/MS on an Ultimate 3000 RSLCnano System (Dionex ...
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bioRxiv - Genomics 2024Quote: ... and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Firefly luciferase activity and Renilla luciferase activity were measured using the Dual-Glo kit (Promega) using a SpectraMax iD5 plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... using Fugene HD (Promega). Controls included wells with empty pACT vector ...
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bioRxiv - Biochemistry 2024Quote: ... The FXR DBD (residues 120-196) and the hinge region (residues 197-243) were cloned into the pACT plasmid (Promega) to generate fusion proteins with the VP16 activation domain ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability assay was performed using Cell titer blue (Promega, Madison, WI). MDS-L cells were seeded in a 96 well plate at a density of 5000 cells / well and treated with different concentrations of PRT543 ranging from 50nM ...
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bioRxiv - Cancer Biology 2024Quote: The target sites for each crRNA were PCR amplified from genomic DNA extracted from tumor-bearing lungs using GoTaq Green® Mastermix (Promega) and the following primer pairs:
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bioRxiv - Cancer Biology 2024Quote: ... were assessed for cell viability by the addition of cell titer blue (Promega). Fluorescence was measured using the Fluostar Omega Microplate reader (BMG lab tech).
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bioRxiv - Neuroscience 2024Quote: ... with 0.5% RNasin Plus (Promega; N2615), 0.5% SUPERase-In (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Firefly luciferase activity was quantified using the ONE-Glo™ EX Luciferase Assay System (Promega, E8130) and read with the Synergy NEO2 multimode reader (BioTek) ...
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bioRxiv - Neuroscience 2024Quote: ... the firefly and renilla luciferase activities were measured using a dual luciferase reporter assay (Promega, Cat. E1960) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
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bioRxiv - Genetics 2024Quote: ... pRLSV40 (Promega), expressing Renilla luciferase ...
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bioRxiv - Biophysics 2024Quote: ... FuGENE® HD transfection reagent was purchased from Promega (Madison, WI). CF®568 succinimidyl ester (CF568-NHS ...
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bioRxiv - Genetics 2024Quote: ... Purified PCR products (deletion-bearing, second-step amplicon) and the pGL4.23 vector (Promega) were double digested with NheI-HF (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... using FuGENE HD Transfection Reagent (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... were cloned upstream of a minimal promoter driven firefly luciferase reporter gene in the pGL4.23 vector (Promega). Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions ...
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bioRxiv - Biophysics 2024Quote: ... 800 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions) cloned in the pGL4.23 vector (Promega) were used as the initial template in PCR ...
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bioRxiv - Genetics 2024Quote: ssDNA constructs generated by M13KO7 helper phage were run on a 1% agarose 1X TBE gel at 100 V for 45 min then size selected ssDNA purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega Madison, WI cat no. PR-A9281) and quantified via Nanodrop 6000 (Thermo-Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Resulting amplicons were purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega cat no. PR-A9281) then cloned into TOPO TA pcR2.1 (Invitrogen cat no ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from NMRI mouse cortical neuron cultures at DIV1 and DIV20 using the Reliaprep RNA Miniprep system (Promega) followed by deoxyribonuclease I (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, # V5117) directly on top of the column and incubating overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 U/mL RNasin (Promega), complete protease inhibitor (Roche)] ...
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bioRxiv - Molecular Biology 2024Quote: ... Dual luciferase reporter assays were performed using the Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Luciferase activity was detected using the GloMax Discover Microplate Reader (Promega). All experiments were done in triplicates.
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with Passive Lysis Buffer and analyzed by dual luciferase assay (Promega). Experiments were performed in triplicate ...
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bioRxiv - Neuroscience 2024Quote: ... and pGag/Pol using FuGENE reagent (Promega, WI, USA). 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were developed with ECL Western Blotting Substrate (#W1001, Promega) or ECL Select Western Blotting Detection Reagent (#RPN2235 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... The gradients were centrifuged in an SW41 rotor at 200,000xg for 1hr Fractions were collected from air-gradient interfaces and complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Blood and iPSC genomic DNA were extracted through Promega Wizard™ Genomic DNA Purification Kits (Promega, Madison, WI, USA). Array comparative genome hybridisation (array-CGH ...
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bioRxiv - Neuroscience 2024Quote: ... (#A1360, Promega) according to the kit manual ...
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bioRxiv - Neuroscience 2024Quote: ... were calculated as the ratio of the fluorescence intensities between the initial (F0) and the last (F) 100 seconds by R-CEPIA1er in the Glomax Discover Microplate Reader (Promega). Caffeine-induced Ca2+ transients were also measured with Fura2 AM at room temperature using an ECLIPSE Ti/L100 epifluorescence microscope (Nikon ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-Halo (1:100; G9211, Promega). Donkey secondary antibodies conjugated to Alexa Fluor 405/488/568/647 (Jackson ImmunoResearch or Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 100 U/ml RNasin (Promega #N2611), and 1x cOmplete EDTA-free Protease Inhibitor Cocktail (Roche #11873580001) ...