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Citations for Promega :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... PCR products from the CAPS analysis were cloned into the pGEM T-easy vector (Promega) and purified using ExoSAP-IT reagent (Affymetrix) ...
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bioRxiv - Neuroscience 2024Quote: ... Luciferase activity was determined at 48 h post-transfection using the Dual-Glo Luciferase Assay System (Promega, Madison, WI; E2940) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... BMSCs were transfected with miR-335-3p mimic, miR-335-3p inhibitor and NC that synthesized by RiboBio (Guangzhou, China) via Fugene HD (Promega, Madison, WI, USA; E2311) when cells confluency reached 50%.
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bioRxiv - Neuroscience 2024Quote: ... using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega: A6020) in the CFX96 Touch Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification of genes utilized GoTaq qPCR Master Mix (Promega) as per the manufacturer’s protocol on the LightCycler® II 480 system machine (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... along with a Ten-m expression construct and Actin-GAL4 using the FuGENE HD transfection reagent (Promega). After 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% TritonX-100) supplemented with protease inhibitor cocktail (Promega). The cell lysates were rotated at 4°C for 30 minutes and sonicated on ice for a total of 1 minute with a “10-second ON ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cholesterol depletion was validated using a Cholesterol/Cholesterol ester-glo assay (Promega: J3190). Endocytosis was blocked using two drugs Dynosore (selleckchem ...
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bioRxiv - Molecular Biology 2024Quote: ... together with standard DNA ladders (1 Kb plus; Promega Corp.) to confirm amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to the cells and incubated for at least 5 minutes on a plate shaker in the dark ...
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bioRxiv - Microbiology 2024Quote: ... Relative light units (RLU) were determined using the GloMax 20/20 Luminometer (Promega). GFP intensity was analyzed using a fluorescence microscope (Mshot ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence from the activity of luciferase was measured at 48 h post-infection using a Tecan Infinite M200 Pro plate reader after applying Bright-Glo reagent (Promega) to the cells.
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bioRxiv - Microbiology 2024Quote: ... Proteins were then solubilized in 50 mM ammonium bicarbonate for a reduction-alkylation step (dithiothreitol 5 mM – iodoacetamide 10 mM) and an overnight digestion with 300ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA). Digested peptides were resuspended in 0.1% formic acid (solvent A ...
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bioRxiv - Microbiology 2024Quote: ... with 8% FBS and 1.6% agarose (Promega). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... supernatants containing trVLPs were collected and the remaining cells were lysed for luciferase assay using the 5x Promega Passive lysis buffer and luciferase activity was measured using a The Dual-Luciferase® Reporter (DLR™) Assay System (Promega, Madison, WI, USA). After confirming that firefly luciferase values remained consistent between conditions ...
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bioRxiv - Microbiology 2024Quote: ... ChIP eluates were purified with Wizard SV Gel and PCR clean-up system (Promega, Cat# A9282). Primer sequences are shown in Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were transfected using Fugene (Promega) with a plasmid encoding the socRNAs of interest ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and subsequent digestion with sequencing-grade trypsin (Promega). To extract the resulting peptides ...
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bioRxiv - Microbiology 2024Quote: ... and luciferase activity was quantified utilizing a commercially available assay kit (Promega). In addition ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase was measured using Brioght-Glo reagent (Promega) approximately 18 hours post- infection ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed using GoScript™ Reverse Transcriptase (Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing grade trypsin/lysC (Promega, Madison, WI) was added in 1:20 (wt/wt ...
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bioRxiv - Microbiology 2024Quote: ... (2) addition of 600μL of Nuclei Lysis Buffer (Promega), and (3 ...
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bioRxiv - Microbiology 2024Quote: Aliquots of the final populations were thawed and the gDNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Microbiology 2024Quote: ... and rehydrated with DNA Rehydration Solution (Promega) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl 5x optimized transcription buffer (Promega), 2 µl T7 RNA polymerase (20 U.ml-1 ...
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bioRxiv - Microbiology 2024Quote: ... 60 µM CTP (Promega, 10 mM stock) and 2-4 µl 10 mCi.ml-1 of radiolabelled CTP [α-32P] ...
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bioRxiv - Microbiology 2024Quote: ... which included a 5 µl nucleotide mix containing 2.5 mM NTPs (Promega). For each reaction ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of 5x optimized transcription buffer (Promega), 2 µl of T7 RNA polymerase (20 U.ml-1) ...
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bioRxiv - Microbiology 2024Quote: ... along with 1.5 µl of rRNasin (40 U.ml-1, Promega), 5 µl of 5x optimized transcription buffer (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... A non-template control consisting of nuclease free-water (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were gel-purified using Wizard® SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 4 Fluorometer (Thermofisher scientific ...
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bioRxiv - Microbiology 2024Quote: DNAase treatment using the kit RQ1 RNase-free DNase (Promega, ref. M6101) was performed ...
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bioRxiv - Microbiology 2024Quote: ... The beads were washed thrice with 180 µl 80 % (v/v) ethanol prior to the addition of the digestion enzyme mix (0.6 μg of trypsin (V5111; Promega, Madison, WI, USA) and 0.6 µg LysC (125-05061 ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was completed by adding 5μL of nuclei lysis buffer (Promega) and 180μL of ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was completed by adding 10μL of nuclei lysis buffer (Promega) and diluting with 100μL of ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... x10 coculture evolved) and gDNA concentration was quantified using the QuantiFluor® ONE dsDNA system kit (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... samples were resuspended in 0.5% SLS with 1 μg trypsin (Promega) and incubated for 30 min at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from 1 ml concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [74] ...
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bioRxiv - Microbiology 2024Quote: ... grown overnight in 50 ml LB with ampicillin and the resulting mutant plasmid pool was purified by midiprep (Promega). The mutant plasmid pool was transformed to BY4742 using the high-efficiency LiOAC/PEG method (Gietz and Schiestl 2007) ...
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bioRxiv - Cancer Biology 2024Quote: ... Authenticity of cell lines was evaluated by detection of ten genetic loci using the GenePrint® 10 System (Promega) and cross referencing to ATCC or internal genetic profiles.
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bioRxiv - Cancer Biology 2024Quote: ... Analyses were carried out using Promega PCR master mix (Promega) on an LC-480 Real-Time PCR system (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... we quantified the luciferase and Renilla signals using the dual-luciferase reporter gene assay kit (Promega, E1960), following the protocol provided by the manufacturer.