Labshake search
Citations for Promega :
1001 - 1050 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... Luciferase activities were measured on a GloMax Luminometer (Promega) using the Steady-Glo Luciferase Assay System (Promega).
-
bioRxiv - Genetics 2024Quote: ... using the Steady-Glo Luciferase Assay System (Promega).
-
bioRxiv - Genetics 2024Quote: ... The CellTiter-Glo® Luminescent Cell Viability Assay by Promega was used to assess the proliferation ...
-
bioRxiv - Genetics 2024Quote: ... and digested on-bead using filter-aided sample preparation with sequencing-grade modified porcine trypsin (Promega) (106) ...
-
bioRxiv - Genetics 2024Quote: ... One µg of total RNA was reverse-transcribed by using the ImProm-ⅡTM Reverse Transcriptase (Promega). Three types of primers were used for the reverse transcription reaction ...
-
bioRxiv - Genomics 2024Quote: ... Cell Titer Glo (Promega G7573) was used to quantify survival 4 days post-transduction ...
-
bioRxiv - Genomics 2024Quote: ... 1x SYBR Green I] and calculated from a standard dilution series of a PCR product (quantified with QuantiFluor® dsDNA System, Promega with a DeNovix DS-11 spectrometer ...
-
bioRxiv - Genomics 2024Quote: ... and quenched with 0.125 M Glycine (Promega) for 5 min ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR was run for 30 cycles using GoTaq G2 Green master mix (Promega) and specific primers ...
-
bioRxiv - Immunology 2024Quote: ... using the Bright-Glo Luciferase Assay System (Promega), according to the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2024Quote: ... using the Bright-Glo Luciferase Assay System (Promega), according to the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2024Quote: Cell viability was determined by the CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7571), the Cell Counting Kit-8 (CCK-8 ...
-
bioRxiv - Genomics 2024Quote: ... pNL3.2 vectors (Promega) were digested and cloned using an Infusion master mix (Takara ...
-
bioRxiv - Genomics 2024Quote: Luciferase assays were performed using a Dual-Glo luciferase assay system (Promega). After 24h ...
-
bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
-
bioRxiv - Genetics 2024Quote: ... and Quantus Fluorometer (Promega, E6150), and then diluted to 0.2 ng/µL ...
-
bioRxiv - Genetics 2024Quote: ... Sample concentration was determined with the Promega QuantiFluor ONE dsDNA System (Promega, E4870) and Quantus Fluorometer (Promega ...
-
bioRxiv - Genomics 2024Quote: ... Cell Titer Glo (Promega G7573) was used to quantify survival after 48 hours of Puromycin selection ...
-
bioRxiv - Genetics 2024Quote: ... and the ATP levels were measured using the bioluminescence detection kit (Promega ENLITEN ATP Assay System, FF2000) and Spectra Max M2 multimode microplate reader (Molecular Devices) ...
-
bioRxiv - Developmental Biology 2024Quote: ... miniata cDNA and cloned into pGEM-T Easy (Promega). Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen) ...
-
bioRxiv - Genetics 2024Quote: ... with on-column DNase I digestion using RQ1 RNase-Free DNase (Promega catalog number M6101).
-
bioRxiv - Developmental Biology 2024Quote: ... NAD+/NADH measurements were performed according to the manufacturer’s instructions (Promega, G9071). In brief ...
-
bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
-
bioRxiv - Genetics 2024Quote: ... 1 μL cDNA was used in 10-μL reactions containing Brilliant III SYBR Green qPCR Master Mix (Promega). Biological replicates were analyzed as duplicates ...
-
bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
-
bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
-
bioRxiv - Developmental Biology 2024Quote: Probes were resuspended in 95μl fresh TE with 5μl RNase inhibitor (Promega: RNasin Plus RNase inhibitor cat:N2611). 5μl aliquots ...
-
bioRxiv - Developmental Biology 2024Quote: ... ORFs for fam83fa and fam83fb were amplified from cDNA libraries and either TA cloned using the pGEM-T Easy Vector System (Promega) or the pENTR/D-TOPO vector system (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 µg of RNA was then used to synthesize cDNA in a 20 µl reverse transcription reaction using 5X First Strand Buffer and MMLV-RT (Promega) with random hexamers ...
-
bioRxiv - Developmental Biology 2024Quote: ... rat anti-Fat (kind gift from Helen McNeill) was used at 1:500 and mouse anti-β-galactosidase (Z3781, Promega; RRID:AB_430877) was used at 1:500 ...
-
bioRxiv - Developmental Biology 2024Quote: ... For RT-qPCR four micrograms of the extracted RNA from each sample were reverse transcribed with gene specific primers using Goscript reverse transcription kit (Promega). Quantitative PCR was performed using Power SYBR Green (Life Technologies) ...
-
bioRxiv - Developmental Biology 2024Quote: ... buffer (containing 10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 1% BSA, 0.1% Tween, 1mM DTT, 1U/ul RNaseIn from Promega, 0.1% NP40, and 0.01% Digitonin) and incubated on ice for 4 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.1 µl 1% digitonin (Promega,#G9441), 0.1 µl 10% Tween-20 (Sigma ...
-
bioRxiv - Genetics 2024Quote: ... Single-stranded cDNA was prepared from the DNase-treated RNA using GoScript Reverse Transcriptase System (Promega).
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using Superscript III Reverse Transciptase (#18080044, ThermoFisher Scientific. qPCR assays were performed on the cDNA using Gotaq Green Master Mix (Promega) following the manufacturer’s instruction and read using CFX96 connect (BioRad) ...
-
bioRxiv - Genetics 2024Quote: ... 6 µL of Fugene 6 (Promega), and OptiMem (ThermoFisher Scientific ...
-
bioRxiv - Genetics 2024Quote: ... in 2x SSCT (0.1% Tween-20 in 2x SSC (Promega, Cat# V4261)) for 5 min at RT ...
-
bioRxiv - Genetics 2024Quote: ... PCR was performed using Go-Taq Green Master Mix (Promega, Madison, WI) and resulting products were resolved by 3% agarose gel electrophoresis ...
-
bioRxiv - Immunology 2024Quote: ... samples were taken and incubated with RealTime-Glo™ MT (Promega) per manufacturer’s instructions in a CO2 and temperature-controlled plate reader (Tecan Spark Cyto) ...
-
bioRxiv - Immunology 2024Quote: ... or Lactate-Glo (Promega) following manufacturers’ protocols ...
-
bioRxiv - Immunology 2024Quote: ... or pMXs-IRES-dsRed-mIRF4 into Plat-E34 with Fugene6 Transfection Reagent (#E2691, Promega) as described in our previous studies35,36 ...
-
bioRxiv - Immunology 2024Quote: Total RNA was extracted from cells using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega) and from skin using RNAzol RT Reagent (#RN190 ...
-
bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
bioRxiv - Immunology 2024Quote: ... CellTiter-Glo 2.0 Cell Viability Assay (Promega Cat. No. G9242) was used according to the manufacturer’s instructions as a measure of proliferation ...
-
bioRxiv - Immunology 2024Quote: ... Each mutation locus was amplified in separate PCR reactions using GoTaq Green Master Mix (Promega Cat. No. M7123). PCR reactions were held at 95 °C for two minutes followed by 35 cycles of 45 seconds at 95°C for denaturation ...
-
bioRxiv - Genetics 2024Quote: ... 40μL of Cell Titer Glo reagent (Promega) was added to each well ...
-
bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
-
bioRxiv - Genetics 2024Quote: ... We utilized the GoScript Reverse Transcriptase Kit (Promega, Madison, Wisconsin, USA) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: RNA from cells was isolated using the ReliaPrep RNA Miniprep System (Promega). RNA was reverse transcribed using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems ...
-
bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...