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Citations for Promega :
701 - 750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were then single cell plated and positive colonies were selected with HaloTag TMR dye (Promega) and verified by PCR of genomic DNA.
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bioRxiv - Neuroscience 2024Quote: ... bIII-tubulin (1:1000, Promega, G7121), LMX1(1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and pGag/Pol using FuGENE reagent (Promega, WI, USA). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed with a Gotaq G2 Hot Start Polymerase system (#M7405, Promega). Pyrosequencing was conducted on a Pyromark Q96 Sequencer using matching reagents (#97204 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA conversion was performed using the GoScript™ Reverse Transcriptase kit (#A5001, Promega). Quantitative real-time PCR was conducted on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNAs were collected from a 10-cm dish directly in 400 μl of 1x Reporter lysis buffer (Promega) containing 0.16 U/μl Ribosafe RNase inhibitors (Bioline) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 30mM HEPES (pH 7.4) and Nano-Glo Live Cell Reagent (Promega), and incubated for 45 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Human male genomic DNA obtained from Promega (Ref:G1471; Madison, WI) was used to generate the input library ...
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bioRxiv - Molecular Biology 2024Quote: ... The level of ATP under different conditions was determined using the Mitochondrial ToxGlo™ Assay from Promega (UK), following the protocol provided by the manufacturer.
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bioRxiv - Molecular Biology 2024Quote: ... The Cas9-sgRNA vector and ssODN were transfected into cells for gene editing using FuGENE HD (Promega, Cat# E2311) transfection reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantification was performed using the Quantus Fluorometer and QuantiFluor RNA Dye (Promega GmbH, Mannheim, Germany). All cDNA libraries were generated from 1 µg total RNA using the TrueSeq Stranded Total RNA Library Preparation Kit with the Ribo-Zero Gold Kit according to the manufacturer’ ss instructions (Illumina Inc. ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts of equal protein amounts were first subjected to proteolysis with trypsin (V5113, Promega) at 37°C for 16 hours ...
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bioRxiv - Neuroscience 2024Quote: ... PCR reactions were performed with GoTaq G2 Flexi DNA Polymerase (Promega, Cat. #M7801) or Herculase II Fusion DNA Polymerase (Agilent ...
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bioRxiv - Genetics 2024Quote: 2000ng DNA (fixed or non-fixed) was mixed with 10μL Brilliant III SYBR Green qPCR Master Mix (Promega) and the DNA melting behaviour was analysed in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: The cell viability was measured every 24 hrs using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, #G4100) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and cell viability was performed using the MTS assay (Promega) 72 hours after initial damaging agent exposure ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 500 ng of Trypsin (V5111, Promega) for an additional 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system.
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bioRxiv - Molecular Biology 2024Quote: ... then 500 ng of Trypsin/LysC (V5071, Promega) for 16 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA isolation was performed by Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor and Maxwell® 16 LEV simplyRNA Purification Kit (PROMEGA, WI, The US) by following the supplier’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were cloned into the pGEM-T Easy vector (#A1360, Promega) and at least 10 clones from each sample were sequenced ...
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bioRxiv - Molecular Biology 2024Quote: ... The viability was measured by using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7572).
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was used to amplify the OGDH 3’UTR and sub clone it into pmirGLO vector (Promega, UK). 5 × 10^4 C2C12 cells were seeded into 12 well plates ...
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bioRxiv - Neuroscience 2024Quote: ... 50 mM triethylammonium bicarbonate and 1 μg of Trypsin/Lys-C mix (Promega) were added ...
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bioRxiv - Microbiology 2024Quote: ... and chromosomal DNA was obtained using the Wizard® Genomic DNA Purification Kit (Promega). DNA fragments were purified from agarose gels using the Zyppy gel extraction kit ...
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bioRxiv - Microbiology 2024Quote: RNA (500 ng) was treated with RQ1 DNase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The resultant PCR products were subsequently subjected to purification through agarose gel electrophoresis employing the Wizard Sv Gel Clean-up system (Promega). Following purification ...
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bioRxiv - Genetics 2024Quote: ... cell lysates were collected and Firefly and Renilla luciferase signals measured using the Dual-Luciferase Reporter Assay System (Promega) as per manufacturer’s protocol on Tecan infinite 200 PRO ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and concentrations assessed using Quantifluor dsDNA assay (Promega). DNA samples were fragmented enzymatically as part of the NEBNext Ultra II FS library preparation workflow.
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bioRxiv - Molecular Biology 2024Quote: ... 0.8 µl of 10 mg/ml BSA (Promega, Madison, Wisconsin, United States), 1 µl of 10 picomolar/µl primers ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg/µl BSA (#R396A, Promega), 10 mM vanadyl-ribonucleoside (VRC ...
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bioRxiv - Molecular Biology 2024Quote: ... 500-1,000 ng of RNA was reversely transcribed using GoScript™ Reverse Transcriptase (Promega) and random hexamers (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were washed with 1X PBS buffer and then lysed with 1X passive lysis buffer (Promega). The cells were cleared of any cell debris by centrifugation at 14000 rpm for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by permeabilization with wash buffer containing Digitonin (Promega). Samples were incubated with rabbit IgG (Millipore) ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 80μL Hank’s balanced salt solution (HBSS) containing 10mM HEPES and 10μM furimazine/NanoGlo Luciferase Assay Substrate (Promega). BRET measurements were performed at 37 °C using a PHERAstar Microplate Reader (BMG Labtech ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Zoology 2024Quote: ... 1 μl 10mM dNTPs (Promega), 2.5 μl of each primer at 10 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by luminescence detection using GloMax Explorer (Promega). The bioluminescence readings were averaged amongst the n=3 technical replicates for each construct and ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... or using Caspase-Glo® 8 Assay Systems® (Promega, G8200) to measure cleaved Caspase 8 activity.
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Biophysics 2024Quote: ... Cells were grown to 70-80% confluency before transfection with β1AR constructs using FuGENE HD reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Then Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega) was used to extract FFPE DNA ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Systems Biology 2024Quote: ... Cell viability was then quantified using CellTiter-Glo (Promega, catalog # G7572) according to the manufacturer protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Cell lines were routinely tested for mycoplasma and were periodically authenticated to its early passage using the GenePrint 10 System (Promega, catalog # B9510). VEM and DAS (Selleck Chemicals ...