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Citations for Promega :
701 - 750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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bioRxiv - Neuroscience 2024Quote: ... genotyping PCR was performed using Go Taq Green Master Mix (Promega, Madison, WI, USA) with the following four primer sets ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg of trypsin (Promega, V5280) was added ...
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bioRxiv - Neuroscience 2024Quote: A DPPIV-Glo Protease Assay Kit (Promega, G8350) was used for DPP9 enzyme activity analysis ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Microbiology 2024Quote: ... Trypsin Gold was purchased from Promega (Madison, WI). Hydrophilic–lipophilic balance (HLB ...
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bioRxiv - Molecular Biology 2024Quote: ... By using CellTiter Blue assay kit (Promega, G8081), 20 μL of CellTiter Blue reagent was added into each well ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Digoxigenin (Dig)-labeled antisense riboprobes were produced using using T7 RNA polymerase by in vitro transcription with RiboMAX Large Scale RNA Production Systems (Promega). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by triple DNase I (Promega) treatment to eliminate any genomic DNA contamination ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 350 µL of this aqueous phase underwent automated extraction and cleaning using a Tecan Freedom EVO liquid handler with Promega Purefood protocol and reagents (cell lysis buffer, ReliaPrep™ resin, prepared wash buffer, isopropanol, 50% ethanol and Promega elution buffer). The total extracted and washed DNA was eluted in a final volume of 150 µL.
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated using the SV Total RNA isolation system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested in 1x passive lysis buffer (Promega) at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega) and a luminometer ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... After this colorization the plate was read using the Glomax multi microplate reader (Promega) at an absorbance wavelength of 560nm ...
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bioRxiv - Neuroscience 2024Quote: ... The coupling with the Halotag-Alexa488 ligand (Promega, G1002) to Nlg1-Halotag was performed by incubating Nlg1-HaloTag with an excess (5X ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of ADP Glo reagent then kinase detection reagent as specified by the manufacturer (Promega). Luminescence was measured in a 384-well plate using a TECAN Infinite M1000 microplate reader (Mannedorf ...
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bioRxiv - Microbiology 2024Quote: ADP-GloTM (Promega) kinase assays were carried out to examine the effect of inhibitors ML-10 and C2 on the activity of recombinant PfPKG proteins ...
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bioRxiv - Microbiology 2024Quote: ADP-GloTM (Promega) kinase assays were also used to determine the Km for ATP ...
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bioRxiv - Neuroscience 2024Quote: ... Afterwards the samples were diluted to decrease the concentration of urea up to 1 M and supplemented with additional amounts of sequencing-grade trypsin (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples) ...
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bioRxiv - Neuroscience 2024Quote: ... and any potential DNA contamination was removed by RNase-free DNase treatment (Promega, M6101). mRNA was reverse-transcribed into cDNA using the high-capacity cDNA reverse transcription kits (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... and 60 U/mL RNasin Plus RNase Inhibitor (Promega, N2611). A 6% IGEPAL CA-630 solution was added to bring the homogenate to 0.3% IGEPAL CA-630 ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Neuroscience 2024Quote: ... and the Caspase-Glo 3/7 assay kit (#G8090, Promega, Nacka, Sweden) were used in 96-well plates (Sarstedt #82.1581.001 ...
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bioRxiv - Neuroscience 2024Quote: ... Absorbance at 450nm was measured using a GloMax® Multimode Microplate Reader System (Promega). Cortisol data from only 70 participants were analyzed due to the limited availability of kits and insufficient saliva samples from some participants.
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bioRxiv - Molecular Biology 2024Quote: ... Glucose uptake assay was obtained from Promega (Madison, WI). phospho-ACLY (Ser455 ...
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bioRxiv - Neuroscience 2024Quote: ... Tunel assay (Promega) was performed following manufacturer’s instructions before staining for MBP and NF ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in reporter lysis buffer (Promega; Table S4). WCE was spotted in a 96-well dish and luciferase activity was measured in arbitrary light units (LU ...
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bioRxiv - Microbiology 2024Quote: ... SL100688) with the exception of BCBL-1 cells that were transfected with FuGENE HD transfection reagent (Promega, Cat. No. E2311,). Competition reporter assays were performed with co- transfection of the TR7 reporter plasmid with or without LANA expression plasmid (DY52) ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Cell viability assays were conducted in 96 well-plate format using Promega’s RealTime-Glo MT Cell Viability Assay (Promega G9712) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted with the Maxwell® RSC simplyRNA tissue kit (Promega) and cDNA was generated with SuperScript II kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Neuroscience 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21ºC overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... using FuGENE HD Transfection Reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... with a GloMax plate reader (Promega). Firefly luciferase activities were normalized based on the Renilla luciferase activity in each well.
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bioRxiv - Neuroscience 2024Quote: ... and Kcan1 genes in a pGL4.11 plasmid (Promega) by Gibson assembly (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: DNA of strain JS3066 was extracted with a Wizard Genomic Purification Kit (Promega, USA). The genome sequencing and assembly was done by the BGI Medical Examination Co. ...
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bioRxiv - Microbiology 2024Quote: ... The reaction consisted of 0.5 µL Luna RT (Promega Cat#E3005S), 2.5 µL of 2X reaction mix ...
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bioRxiv - Microbiology 2024Quote: ... 5ng of renilla luciferase (pRL-TK - Promega E2241), and 20ng of each putative PDE using the Mirus TransITX2 transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... CellTiter-Glo (Cat# G7570, Promega) was resuspended with buffer and added 1:1 vol/vol directly to the media and placed 2 min on an orbital shaker ...
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bioRxiv - Microbiology 2024Quote: ... using DNA extracted from the relevant organisms with the Wizard® genomic purification kit (Promega) according to manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... and measured in a GloMax Explorer (Promega) luminometer.
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).