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Citations for Promega :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Luminescence of the fractions was measured with a luminometer (Promega, GloMax Explorer). EV associated peak fractions were 2-fold serially diluted in PBS and analyzed by nanoparticle tracking analysis (Nanosight NS300 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Fractions were complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Luminescence of the fractions was measured with a luminometer (Promega, GloMax Explorer). EV-associated HiBiT-Arc was identified in gradient fractions 15-17 as these fractions have a density consistent with the known densities of EVs (1.10-1.15g/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... After cDNA conversion with a GoScript™ Reverse Transcriptase kit (#A5001, Promega), quantitative real-time PCR was performed on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Pathology 2024Quote: ... Lys-C (Promega) was added in an enzyme:substrate ratio of 1:75 for 4 hours at 25 °C ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 100 mM HEPES pH 7.5 and digested at 37°C overnight with Trypsin/Lys-C (Promega) at a protease-to-proteome ratio of 1:100 (wt/wt) ...
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bioRxiv - Neuroscience 2024Quote: ... corresponding HRP-conjugated secondary antibodies (Anti-Rabbit, #W4011 and Anti-Mouse #W4021, both from Promega) were incubated (1:4,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (sequencing grade, Promega) at 1:100 (enzyme ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... GoTaq qPCR Master Mix (Promega, Madison, WI, USA). Gene expression changes were normalised to 36B4 or Ppia gene expression by using the ΔΔCT method.
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bioRxiv - Neuroscience 2024Quote: ... The luminescence readings were acquired using a GloMax Microplate Reader (Promega). The percentage of viability was determined by normalizing the raw luminescence values to those of the vehicle condition (DMSO-treated wells ...
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bioRxiv - Neuroscience 2024Quote: ... viability was assessed with an ATP-based chemiluminescent assay (Cell Titer-Glo®, Promega; Cat#G7570) following the manufacturer’s instructions ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and nanoluc substrate (Promega, 1:200) diluted in 1X passive lysis buffer (Promega) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Arc-Hibit signal was identified by complementation with LgBiT (equal volumes) and measurement of Nanoluc-mediated luciferase [JDS1] (Nano-Glo Luciferase Assay, Promega, 565nm) on a microplate reader (SpectraMax iD3).Total Arc in the cell lysate is calculated by dividing the luciferase value of Arc cell lysate by the % of Arc lysate sample used from total sample collected ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized with AttoPhos (Promega) and scanned with an FLA-5000 fluorescent image analyzer (Fujifilm) ...
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bioRxiv - Molecular Biology 2024Quote: ... By using CellTiter Blue assay kit (Promega, G8081), 20 μL of CellTiter Blue reagent was added into each well ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... and Cd68 were quantified by using GoTaq PCR master mix (Promega, Madison, WI) in OneStep Plus qPCR (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (1000ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript reverse transcriptase kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Molecular Biology 2024Quote: ... Digoxigenin (Dig)-labeled antisense riboprobes were produced using using T7 RNA polymerase by in vitro transcription with RiboMAX Large Scale RNA Production Systems (Promega). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNAsin Plus (Promega) and vortexed ...
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bioRxiv - Immunology 2024Quote: ... and 1 μg of RNA used as a template for cDNA synthesis with random primers and ImProm-II reverse transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... CellTiter-Glo (Promega, G9241) was added to the media ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing-grade trypsin (Promega, 1:25 enzyme:protein ratio) diluted in 20 µl digestion buffer (50 mM TEAB ...
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bioRxiv - Immunology 2024Quote: ... To promote transfection mixes were supplied by Fugene HD transfection reagent (Promega Madison WI). On the third day ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µL of digestion solution (1.25 mM TCEP, 5 mM chloroacetamide, 0.2 µg trypsin/Lys-C mix (Promega, cat#V5073), in 100 mM HEPES pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... LDH activity was assayed using a CytoTox 96 assay kit (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and luminescence was measured using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and then a final solution comprised of 17% formamide + 5% Triethalomine + 70% glycerol + 3% Tris (w/v, Tris, Promega, H5135) made in basic DDH20 ...
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bioRxiv - Molecular Biology 2024Quote: ... the relative luciferase activity was measured according to manufactureŕs instructions (Promega, #E1910). The relative luciferase activity was calculated by the ratio of firefly luciferase activity to internal Renilla luciferase activity.
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bioRxiv - Molecular Biology 2024Quote: ... each WT ZNF410 peak was amplified by PCR from genomic DNA and cloned into pGL4.24 vector (Promega, #E8421), digested by KpnI and XhoI ...
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bioRxiv - Molecular Biology 2024Quote: Each ZNF410 peak and all the variants of ZNF410 motif mutant sequences were sub-cloned into pGL4.24 plasmid (Promega, #E8421). About 1.2 μg WT or constructed pGL4.24 plasmid and 0.2 μg pGL4.74 control plasmid (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... About 1.2 μg WT or constructed pGL4.24 plasmid and 0.2 μg pGL4.74 control plasmid (Promega, #E6921) were co-transfected into K562 cells by Neon™ NxT Electroporation System (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega), 0.125 µCi/µL 35S-methionine (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega) and 0.125 mg/mL of home-made recombinant T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 U/µL RNase inhibitor (Promega), 0.125 µCi/µL 35S-methionine (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μL of diluted Nano-Glo reagent (Promega, Walldorf, Germany) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of CellTiter-Blue reagent (Promega) was then added to each well ...
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bioRxiv - Immunology 2024Quote: ... permeabilized with 1% TRITON X-100/PBS (Promega) and blocked with 5% FBS/PBS ...
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bioRxiv - Immunology 2024Quote: ... Digitonin (Promega) and Proteinase K (Roche) ...
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bioRxiv - Immunology 2024Quote: ... 75 µL of Bio-Glo luciferase reagent (Promega) was then added to each well and the luminescence was read in a Synergy 4 (BioTek ...
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufactureŕs protocol.
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bioRxiv - Molecular Biology 2024Quote: LDH release was quantified according to the manufacturer’s recommendations (LDH-Glo Cytotoxicity Assay, Promega, Madison, WI). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Amplicons were cloned into pGEM-T-Easy (Promega, A1360) after adding an A-overhang with Taq DNA polymerase (New England Biolabs ...