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Citations for Promega :
8151 - 8200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). 250 ng of genomic was used in each PCR1 amplification with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... FuGENE 6 (Promega) transfection reagent was used according to the manufacturer’s instructions to transfect at a 3:1 ratio (using 1.5 µL FuGENE 6 and 0.5 µg DNA per well) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The first-strand cDNA was converted using a reverse transcription system (Promega, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... lactate dehydrogenase (LDH) release assay was performed using CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega, Madison, Wisconsin, USA) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... a mix was prepared consisting of 5 % PEG-8000 (Promega), 100 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted using the Wizard Genomic DNA purification kit (Promega, USA) and stored at −20 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 16S rRNA gene was amplified using primers 27F-YM 5’-AGAGTTTGATYMTGGCTCAG-3’ and 1391R 5’-GACGGGCGGTGWGTRCA-3’ and GoTaq DNA Polymerase (Promega, USA). The PCR was performed as follows ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then performed tissue lysis and DNA isolation using the Wizard SV Genomic DNA Purification kit (Promega). We fluorometrically quantified DNA using the PicoGreen dsDNA kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the cells were lysed using a passive lysis buffer (Promega) and equal amounts of lysates were used for luciferase reporter assay following the manufacturer’s instructions (Promega).
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bioRxiv - Evolutionary Biology 2023Quote: ... or a Maxwell® 16 LED DNA kit (Promega, Madison, WI, USA) according to standard procedure ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and equal amounts of lysates were used for luciferase reporter assay following the manufacturer’s instructions (Promega).
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bioRxiv - Evolutionary Biology 2023Quote: ... was combined with 9.6 μL FUGENE 6 (Promega #E2691) and incubated for 5 minutes ...
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bioRxiv - Genetics 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (Casabona et al. ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Genomics 2023Quote: ... and 0.5 U ml−1 RQ1 DNase I (Promega, M6101). The samples were incubated at 37 °C in successive 10-minute rounds with gentle vortexing every 2 minutes and trituration every 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Each product was cloned into the pANT vector (Nippon Gene) or pGEM-T easy (Promega) and sequenced with M13F-pUC or M13R-pUC primers (Table S3).
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bioRxiv - Developmental Biology 2023Quote: ... pGL4.74 containing the Renilla luciferase gene was used for normalization of reporter expression (Promega, E6921). Coding sequences of mouse Hoxd13 ...
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bioRxiv - Developmental Biology 2023Quote: ... Luciferase activity in the cell lysates was measured using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: The highly conserved 998-bp core sequence of Xenopus tropicalis MFCS1 was amplified by PCR with specific primers (Table S3) and inserted into the Acc65I and XhoI sites of the pGL4.23 vector (Promega, E8411). Then the minimal promoter of the pGL4.23 vector was replaced with a 486-bp sequence of Xenopus laevis shh promoter (Fig ...
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bioRxiv - Developmental Biology 2023Quote: ... hes7.1 and hes2 constructs were cloned from total reverse-transcribed cDNA into pCS107 for overexpression and/or pGEM-T Easy (Promega #A137A) for anti-sense probes using primers listed below ...
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bioRxiv - Developmental Biology 2023Quote: ... the QuantiFluor™dsDNA System from Promega was used ...
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bioRxiv - Developmental Biology 2023Quote: ... and synthesized with T7 (Promega #P207E) mRNA encoding membrane-GFP was used in some experiments as lineage tracers at 50ng/µL ...
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bioRxiv - Developmental Biology 2023Quote: ... All mRNAs were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B).
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of DNA was performed using GoTaq (Promega, Cat# M7123) or Phusion (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... were DNAse treated (Promega), and were further cleaned with an RNA clean and concentrator kit (Zymo Research) ...
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bioRxiv - Developmental Biology 2023Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910). Luminescence was measured using a microplate reader (TECAN infinite M200pro) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ghost Database KY21.Chr1.1715) we extracted genomic DNA from local Norwegian animals using the Wizard Genomic DNA Purification Kit (A1120, Promega). We then used the purified gDNA at a concentration of 100-150ng/μl ...
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bioRxiv - Developmental Biology 2023Quote: ... Q-PCR was performed using GoTaq qPCR Master Mix (Promega) with the carboxy-X-rhodamine (CXR ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Developmental Biology 2023Quote: ... including the identified m6A site (wildtype sequence or A-to-C mutation) was cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega), per manufacturer ’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was completed using Applied Biosystems QuantStudio3 Real-Time PCR system using GoTaq qPCR Master Mix with SYBR Green (Promega). All primers used for RT-qPCR are shown in Table S3 ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μl suspended streptavidin MagneSphere beads (Promega #Z5481) per sample were washed 3×5 minutes in 0.5x SSC ...
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bioRxiv - Immunology 2023Quote: ... or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice and incubated in RPMI with 5 mM sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA (cDNA) was generated using 1 μg RNA using M-MLV Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... destained and digested in-gel with 200 ng modified trypsin (sequencing grade, Promega) and Rapigest (TM ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed on the plate and incubated with luminescence glo regents per the luminescence glo assay kit’s instructions (Promega). Luminescence was recorded using a BioTek Synergy2 plate reader.
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bioRxiv - Immunology 2023Quote: ... The quantity of RNA was analyzed with the Quantus Fluorometer (Promega, Madison, USA). RNA quality control was done using the 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... Tissues were embedded in 3% low melting point agar (Promega). Formalin embedding ...
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bioRxiv - Immunology 2023Quote: ... and RNAsin (Promega). Reverse transcription and a first PCR was performed as described above (50 cycles ...
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bioRxiv - Immunology 2023Quote: HTR cells were transfected with the indicated plasmids using TransFast transfection reagent (Promega,Madison, WI, USA) according to the manufacturer’s protocol as previously described [9] ...
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bioRxiv - Immunology 2023Quote: ... and then subjected to overnight digestion at 37°C with sequencing-grade modified trypsin (Promega, Madison, WI, USA). The peptides were extracted ...
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bioRxiv - Immunology 2023Quote: ... Luciferase Assay System (E1501) and Passive Lysis 5 × Buffer (E1941) were purchased from Promega. C1q recombinant protein (A400 ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed with BrightGlow Lysis Reagent (Promega E2620) and the mean relative luminescence units (RLU ...
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bioRxiv - Immunology 2023Quote: ... RNA was treated with RQ1 RNase-Free DNase (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) release to the culture supernatant was quantified by using the LDH cytotoxicity assay kit (Promega).
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bioRxiv - Immunology 2023Quote: ... Cell cytotoxicity was measured using the CytoTox96 Non-radioactive Cytotoxicity Assay (Promega) and expressed as a percentage of total cellular LDH (100% lysis control) ...
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bioRxiv - Immunology 2023Quote: Proliferation of MC38 cells was measured using the CellTiter-glo 2.0 Assay (Promega) according to manufacturer’s instructions ...