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Citations for Promega :
6651 - 6700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1.2 μl of MgCl2 (25 mM) and 0.1 μl of Taq polymerase (Promega, Madison, WI, USA, 1U/μl), 8.7 μl H2O ...
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bioRxiv - Microbiology 2023Quote: Total RNA from colon tissues was isolated using Maxwell® 16 LEV simplyRNA tissue kits (Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Digestion was performed first with Endoprotease LysC (Promega) and then with trypsin (ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... following the manufacturer protocol (Promega). Luciferase values were normalized by the corresponding ENV levels ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNAsin (Promega)) as previously described41 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with tRNA and RNasin (Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µl of bacteria were mixed with 50 µl of BacTiter-Glo™ (Promega) and incubated at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of DNAsed RNA were retro-transcribed using random hexamer primers with the M-MLV-RT enzyme (Promega). cDNA were amplified with the GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2023Quote: After coelenterazine H (Coel H, Promega, 2.5 µM) addition ...
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... treated with RQ1 Rnase-free Dnase (Promega), and converted to cDNA using the Promega Reverse Transcription system (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA were amplified with the GoTaq qPCR Master Mix (Promega) and the fluorescence was measure on a QuantStudio 6 Flex System (Applied Biosystems) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... and converted to cDNA using the Promega Reverse Transcription system (Promega). SYBR green RT-qPCR was performed in triplicate using cDNA and run on the Bio-Rad CFX384 real time thermocycler ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability and caspase 3/7 activity was measured with CellTiter Flo (Promega) or CaspaseGlo (Promega ...
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bioRxiv - Molecular Biology 2023Quote: Cell proliferation was measured with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: The P450-Glo™ CYP2D6 Assay and P450-Glo™ CYP3A4 Assay Luciferin-IPA (Promega) kits were used to measure Cytochrome P450 2D6 and P450 3A4 activity employing a luminometer (Lumat LB 9507 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2µg of total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... abd a GloMax plate-reader (Promega). Samples were prepared by boillling with 2x Laemmli sample buffer (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: Luciferase activity was analyzed 48 h post-transfection using DualGlo (Promega), essentially as previously described (25) ...
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bioRxiv - Molecular Biology 2023Quote: ... the strips were washed in TBS-T for 15 min and incubated with the secondary antibody anti-mouse IgG-HRP (Promega, Cat. no: W4028) at 1:10000 dilution in TBS-T 3 % BSA for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ng/µL trypsin (Promega, USA) in water to saturate the inner surface and to prevent sample loss by nonspecific adsorption ...
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bioRxiv - Molecular Biology 2023Quote: ... Reduction, alkylation and in-gel digestion were performed as described (Shevchenko et al., 2006) except that Trypsin/LysC mix (Promega) was used instead of trypsin ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with CellTiter 96® AQueous One Solution Cell Proliferation Assay reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed and luciferase emission was measured using Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Molecular Biology 2023Quote: ... primer extension reactions were done using AMV reverse transcriptase (Promega) and 32P-labeled primer (Pisarev et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Firefly luciferase activity was quantified using the Dual-Luciferase Reporter Assay System (Promega) and normalized against Gaussia luciferase activity.
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bioRxiv - Molecular Biology 2023Quote: ... The digoxigenin-labeled antisense and sense sybu riboprobes were synthesized from a SacII-or SacI-linearized pGEM-T easy (Promega), which contained an 823 bp cDNA-fragment from sybu-mRNA (NCBI Reference Sequence ...
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bioRxiv - Molecular Biology 2023Quote: Native total calf liver tRNA (Promega) and in vitro transcribed tRNAiMet were aminoacylated using recombinant E ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Neuroscience 2023Quote: ... and RNase inhibitor (Promega N2515). We designed male-specific and female-specific forward RT-PCR primers ...
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bioRxiv - Immunology 2023Quote: ... Caspase-3/7 activity was detected using the Caspase-Glo 3/7 Assay Kit (Promega) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... ATP was detected using the CellTiter-Glo 2.0 Assay Kit (Promega) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... The samples were then diluted with Tris-HCl (100 mM, pH 8.0) and digested with 0.5 µg sequencing-grade trypsin (Promega). The generated peptides were desalted and analyzed in duplicates by LC-MS/MS using a timsTOF fleX mass spectrometer equipped with a nanoElute LC system (Bruker) ...
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bioRxiv - Genomics 2023Quote: ... following the recommendations of the manufacturer’s capping protocol (https://international.neb.com/protocols/0001/01/01/capping-protocol-m2080) with two changes: 3.5 µl of RNAse inhibitors (RNasin Plus RNase Inhibitor, catalog num N2611, Promega) were added to the capping reaction to avoid RNAse degradation ...
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bioRxiv - Genomics 2023Quote: The RNase ONE Ribonuclease (catalog num. M4261, Promega) was used at this point to eliminate the Biotin groups at the 3’ end by degrading only the single-strand RNA present into the mixture ...
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bioRxiv - Genomics 2023Quote: ... and 1% unmethylated lambda DNA (cat#: D1521, Promega) was spiked in to measure bisulfite conversion efficiency ...
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bioRxiv - Microbiology 2023Quote: ... and AMV Reverse Transcriptase (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... GluC (Pierce) or Chymotrypsin (Promega) at a concentration of 12.5 ng/µl in 50 mM AB ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with 10µM Coelenterazine-h (Promega) in the dark for 2 hours before recording luminescence in a plate reader (Optima FLUOStar ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tg(elavl3:EGFP)knu3 incross were individually placed in wells of 24-well plates in water containing 0.5 mM beetle luciferin (Promega). From ZT14 (the light to dark transition ...
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bioRxiv - Neuroscience 2023Quote: LDH release from macrophages treated with Aβ profect complexes was measured using the CytoTox-ONE Homogeneous Membrane Integrity Assay (cat no: G7891, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay, Promega, Catalog #G7570). A subset of cells was rinsed twice with phosphate buffered saline and incubated with lysis buffer (1M Tris HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2023Quote: ... 12.5 µl 40 U/ µl RNasin (Promega Cat# N2615), 10 µl protease and phosphatase inhibitor cocktail (100X ...
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bioRxiv - Microbiology 2023Quote: The protein was digested overnight with 0.1 μg LysC (Promega) and 0.8 μg trypsin (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was subsequently confirmed using sanger sequencing and transcribed in vitro using T7 RNA polymerase (Promega) and Digoxygenin (DIG ...
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bioRxiv - Microbiology 2023Quote: Approximately 100 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert ...