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Citations for Promega :
6451 - 6500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 5 μM Bright-Glo (Fluc Luciferase Assay System, Promega) was then added to the wells ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNA samples were treated with DNase I (Promega, Madison, WI, USA) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: We used the dual-luciferase psiCHECK2 system (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 40 μl 2.5 ng/μl trypsin (V5111 sequencing grade modified trypsin, Promega) for overnight digestion at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (1.2 µg) was utilized to reverse transcript with M-MLV Reverse Transcriptase kit (M1701, Promega), Random Hexamer Primers (48190011 ...
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bioRxiv - Pathology 2023Quote: ... Absorbance was measured at 450 – 540 nm using the GloMax® Explorer Multimode Microplate Reader (#GM3500, Promega).
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bioRxiv - Neuroscience 2023Quote: TUNEL assay: DeadEND Fluorometric TUNEL system kit was purchased from Promega (cat. # G3250) and TUNEL assay was performed according to the manufacturer’s description ...
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bioRxiv - Systems Biology 2023Quote: ... Luciferase assays were performed using luciferase assay system (E1500, Promega) and activity measured using a Sirius luminometer (Tiertek Berthold) ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... CellTiter-Glo Luminescent Cell Viability Assay was purchased from Promega. Pico488 dsDNA Quantification Reagent and the dsDNA quantitative standard (100 ng/µL ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Systems Biology 2023Quote: ... genes were cloned in pFN19A (HaloTag®7) T7 SP6 Flexi® vector (Promega, USA) by Gibson assembly (Gibson et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-fusion proteins were purified with Magne® HaloTag® Beads (Promega, USA) and then incubated with 50 ng ampDAP library ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were digested using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Microbiology 2023Quote: ... Luminescence readings were performed using a Glomax 20/20 Luminometer system (Promega). Each experiment contained a minimum of three technical replicates and experiments were repeated at least three times ...
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bioRxiv - Biophysics 2023Quote: ... we used the organic dye JF 646 conjugated to the HaloTag ligands (Promega). For conventional imaging of Atg13 (Supplementary Fig ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and 135uL Fugene transfection reagent (Promega). The vector structure was as follows ...
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bioRxiv - Biochemistry 2023Quote: ... and BL21 (DE3) pLysS (Promega, L1195) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... pGlosensor-22F (Promega). Surface expression levels of constructs were titrated to similar levels with doxycycline and measured by flow cytometry using an Alexa-647 conjugated anti-M1 Flag antibody ...
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bioRxiv - Biochemistry 2023Quote: The recruitment of PTH1R to β-arrestin was detected in HEK293 cells using the NanoLuc Binary System (NanoBiT; Promega). The Lgbit subunit was fused to the C-terminus of PTH1R and the SmBiT subunit was fused to the N-terminus of β-arrestin ...
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bioRxiv - Biochemistry 2023Quote: ... Nano luciferase (NLuc) was expressed from pNL1.1.PGK (Promega). Constructs for protein expression in E.coli were cloned with an N-terminal 6xHis-tag into pET15b ...
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bioRxiv - Biochemistry 2023Quote: VIP2R and CRF2R were constructed into the pcDNA6.0 vector (Promega) for cAMP accumulation ...
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bioRxiv - Biochemistry 2023Quote: ... the transfection potency of mLuc was measured using the One-Glo Luciferase Assay System (Promega), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase activity was measured using Luciferase Assay reagent (Promega) and analyzed on a Biotek Synergy Neo2 plate reader.
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bioRxiv - Biochemistry 2023Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R with a 17-amino acid linker ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM Tris-HCl (pH7.5)) supplemented with proteinase inhibitor tablet and 10 µl RNasin (Promega). Fresh puromycin was added to a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were diluted to a final concentration of 1e6 cells per mL in Expi293 expression medium plus 2% (v/v) Glosensor assay reagent (Promega) and intubated for 75 min at room temperature with gentle rotation ...
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bioRxiv - Biochemistry 2023Quote: ... sequencing-grade trypsin (Promega, V5117) was added to each sample at a ratio of 1:20 and allowed to digest overnight at 37 °C.
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from cell culture using Maxwell RSC simplyRNA Cells Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-HiBiT western blot was then performed with 1:5,000 dilution of mouse anti-HiBit (Promega, Clone 30E5) and 1:10,000 dilution of goat anti-mouse-HRP secondary antibody (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... was from American Radiolabeled Chemicals Inc. (St. Louis, MO, USA) and PCR reagents and restriction enzymes from Promega Corporation (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Biochemistry 2023Quote: ... and real-time quantitative PCR experiments were performed using SYBR Green (Promega) in CFX connect Real-Time PCR detection system (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence values were normalized by amount of live cells in vehicle control-treated wells (CellTiter-Glo 2.0 Assay Kit, Promega). CTG reagent was added to vehicle control-treated wells in triplicate 15 min after addition of the cAMP-glo Max Assay kit ONE buffer.
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bioRxiv - Bioengineering 2023Quote: ... V Variant (Promega, Cat#G7015) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... GFP intensity of cells treated with each drug library was measured by the fluorescence microreader (GloMax Discover, Promega). The inhibition rate for GFP intensity was calculated ...
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bioRxiv - Biochemistry 2023Quote: TPCK-treated sequencing-grade modified trypsin was obtained from Promega (Madison, WI). UltraPure™ Tris Buffer was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2023Quote: GsaB-modified GsaA precursor protein was digested with trypsin (Promega, sequencing-grade) at 37 °C for 20 min. ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Cancer Biology 2023Quote: ... + 0.1% RNasin Plus RNase Inhibitor (Promega) for 15-30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo® 3D cell viability reagents (Promega) were used to analyse spheroid viability as per manufacturer’s instrcutions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.2U/µl RNAsin (Promega, cat.no N2615)) were added to the sections and the tissue was cut into small pieces using scissors ...
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bioRxiv - Biochemistry 2023Quote: ... using 3 µl of the in vitro translation reaction mixture in 150 µl of luciferase assay reagent (Promega, Fitchburg, WI). The graphs in Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected by using SYBR Green I (GO-Taq PCR Master mix, Promega, Walldorf, Germany; A6002). Suitable reference genes were identified by using geNorm [47] ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell viability was measured by CellTiter-Glo assay (Promega). To assess in vivo gene silencing ...