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Citations for Promega :
6851 - 6900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... then harvested for genomic DNA using the Wizard® Genomic DNA Purification Kit (Promega). Genomic DNA was sent for whole- genome sequencing at the Mutualized Platform for Microbiology at Institut Pasteur.
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bioRxiv - Microbiology 2023Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity using a CellTiter-Glo assay (CTG, Promega) at 48 hours post treatment ...
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bioRxiv - Microbiology 2023Quote: ... and so proteins were instead digested using sequencing grade gluC (Promega Corporation) added to the solution in a 1:20 mass ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... FuGENE HD reagent (Promega, #E2311) was used for the co-transfection ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rRNA region was amplified with the standard bacterial primers 335F and 769R with GoTaq Mastermix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... in a GloMax navigator luminometer (Promega). Data are expressed as normalized ratios (firefly:Renilla signal) ...
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bioRxiv - Molecular Biology 2023Quote: Firefly and Renilla luciferase activity was measured in cell lysates using the Dual-Luciferase Reporter assay system (Promega) in a GloMax navigator luminometer (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... following the instructions of the supplier and the digested vector was dephosphorylated by TSAP enzyme to avoid its recircularization (Promega, Madison, WI, USA). After electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega, https://www.promega.com). Three biological replicates for each type of sample were used for sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNA samples were treated with DNase I (Promega, Madison, WI, USA) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Cell viability assay was performed using CytoTox-Glo™ Cytotoxicity Assay (Promega) as suggested by the manual.
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from the selected monoclonal population (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI) following the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... were synthesised by in vitro transcription using the RiboMAX™ system (Promega, Cat # P1300) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 40 μl 2.5 ng/μl trypsin (V5111 sequencing grade modified trypsin, Promega) for overnight digestion at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (1.2 µg) was utilized to reverse transcript with M-MLV Reverse Transcriptase kit (M1701, Promega), Random Hexamer Primers (48190011 ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were digested using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Neuroscience 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Synthetic Biology 2023Quote: ... backbone to contain the large or small fragments of the NanoBiT luciferase (Large BiT or Small BiT) used for the split luciferase complementation assay (Promega, Madison, WI, USA)38 ...
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bioRxiv - Plant Biology 2023Quote: ... The NanoGlo® Dual-Luciferase® Reporter Assay System (Promega N1610) was used to quantify NanoLuc and LucF luminescence in a CLARIOstar Plus plate reader with each well measured for ten seconds at 3600 gain with one second settling time.
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bioRxiv - Plant Biology 2023Quote: ... Samples were ground to fine powder using pestles on dry ice and then 100 μl 2x cell culture lysis buffer was added (Promega, E1531; with 150 mM Tris-Hcl pH7.5). 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were lysed with Luciferase Cell Culture Lysis Reagent (Promega) and luciferase activity was measured according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified fragment cloned into pGEM-T Easy vector (Promega K. K., Tokyo, Japan) was used to synthesize DIG-labeled sense and antisense RNA probes using DIG RNA labeling kit (SP6/T7 ...
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bioRxiv - Plant Biology 2023Quote: ... Horseradish peroxidase-conjugated antibodies (anti-rabbit and anti-mouse; Promega) were applied followed by chemiluminescent ECL detection (Amersham ...
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bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.
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bioRxiv - Systems Biology 2023Quote: ... genes were cloned in pFN19A (HaloTag®7) T7 SP6 Flexi® vector (Promega, USA) by Gibson assembly (Gibson et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-fusion proteins were purified with Magne® HaloTag® Beads (Promega, USA) and then incubated with 50 ng ampDAP library ...
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bioRxiv - Systems Biology 2023Quote: ... Luciferase assays were performed using luciferase assay system (E1500, Promega) and activity measured using a Sirius luminometer (Tiertek Berthold) ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... CellTiter-Glo Luminescent Cell Viability Assay was purchased from Promega. Pico488 dsDNA Quantification Reagent and the dsDNA quantitative standard (100 ng/µL ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Pathology 2023Quote: Drug cytotoxicity was measured by the lactate dehydrogenase (LDH)-GloTM Cytotoxicity Assay Kit (#J2380, Promega) following manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The GoTaq G2 Polymerase (Promega) was used for genotyping PCR using PCR primers as listed in Table S1.
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bioRxiv - Pathology 2023Quote: ... Luminescence was measured at 400 nm using the GloMax® Explorer Multimode Microplate Reader (#GM3500, Promega).
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bioRxiv - Pathology 2023Quote: ... Absorbance was measured at 450 – 540 nm using the GloMax® Explorer Multimode Microplate Reader (#GM3500, Promega).
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bioRxiv - Neuroscience 2023Quote: TUNEL assay: DeadEND Fluorometric TUNEL system kit was purchased from Promega (cat. # G3250) and TUNEL assay was performed according to the manufacturer’s description ...
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bioRxiv - Physiology 2023Quote: ... DNA was extracted from each sample using the Wizard Genomic DNA Purification Kit (Promega). Quality of DNA samples were assessed on a 1.0 Nanodrop (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... and was subsequently treated with RQ1 DNAse (Promega). 150 ng (P ...
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bioRxiv - Plant Biology 2023Quote: ... and HiBiT Control Protein (N3010, Promega). We were unable to obtain soluble protein of AtARF9 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein yield was quantified by measuring luminescence with the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010 ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) 18 primers and the GoScript™ Reverse Transcriptase (Promega). qRT-PCR was performed using gene-specific primers (Supplementary Table 1 ...
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bioRxiv - Systems Biology 2023Quote: ... Dual luciferase reporter activity was measured using the Nano-Glo Dual-Luciferase Reporter assay (Promega, N1630). The luciferase activity data shown is relative to the control pGL4.S4 luc TK activity ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were incubated for one hour at 47 oC with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (w/w ...
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bioRxiv - Zoology 2023Quote: ... then ligated to pGEM-T easy vector (Promega, USA) prior to transforming Escherichia coli-DH5α (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: We used the dual-luciferase psiCHECK2 system (Promega) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... and digested by 10 ng trypsin (Promega) at 37 °C for 60 min ...