Labshake search
Citations for Promega :
6501 - 6550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... in a GloMax Multi Plus Multiplate Reader (Promega, Walldorf, Germany) according to the preset protocol with an integrity time of 10 seconds for each read ...
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bioRxiv - Cancer Biology 2023Quote: ... Dual-Luciferase Reporter Assay (Promega, Walldorf, Germany, E1910) was conducted in accordance with the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected using SYBR Green I (GO-Taq PCR Master mix, Cat: A6002; Promega, Walldorf, Germany). Sequences of the primers used in this study are listed in Supplementary Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: Mouse and human MIR122 promoter regions were amplified from genomic DNA and cloned in the pGL4.10[luc2] promoter-less luciferase plasmid (Promega, Walldorf, Germany,). Restriction sites for NheI ...
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bioRxiv - Cancer Biology 2023Quote: ... Promegás Dual-Luciferase Reporter Assay (Promega, Walldorf, Germany) was conducted in accordance with the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The pGL4.32[luc2P/ NF-κB-RE/Hygro] vector for NF-κB luciferase reporter assays was purchased from Promega. shRNA constructs targeting BRD2 were purchased from Sigma (Mission shRNA).
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured by ATP content using the Cell Titer-Glo luminescent assay kit (Promega). For cell imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... the assay was performed using the Bio-Glo Luciferase Assay System (Promega). The following experimental controls of TCR transfection were included ...
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bioRxiv - Biochemistry 2023Quote: ... 200 ng of Trypsin/Lys-C mix (Promega) was added ...
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bioRxiv - Physiology 2023Quote: ... Samples were incubated for 60 minutes at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (w/w ...
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bioRxiv - Physiology 2023Quote: ... and luciferase reporter assays were carried out using the Dual-Glo Luciferase Assay System (Promega).
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bioRxiv - Synthetic Biology 2023Quote: ... and assayed using the ONE-Glo EX luciferase assay system (Promega) on a SpectraMax L luminometer (Molecular Devices ...
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bioRxiv - Genetics 2023Quote: ... Indexed samples were pooled and quantified by fluorometric quantification (QuantiFluor® dsDNA System, Promega) and visualized on QIAxcel Capillary Electrophoresis System (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: ... RNA samples were treated with RQ1 RNase-Free DNase (Promega M6101) and cleaned up using a Zymo RNA clean and concentrator kit.
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bioRxiv - Bioengineering 2023Quote: ... and reverse transcribed into complementary DNA with Gotaq Reverse Transcriptase (Promega) using a reverse primer matching on the 3’and of viral mRNA ...
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bioRxiv - Bioengineering 2023Quote: ... RNA samples prepared were treated with DNase according to the manufacturer’s instructions (RQ1 RNase-Free DNase from Promega), quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Genetics 2023Quote: ... a reverse transcriptase produced by Promega (USA), was used to synthesize cDNA ...
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bioRxiv - Cell Biology 2023Quote: The caspase 1 glo (Promega; cat. G9951) was followed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... while cell viability was measured using CellTiter-Glo (Promega). Bortezomib and DMSO-only wells were included as positive and negative controls ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell viability was measured using CellTiter-Glo (Promega). Bortezomib and DMSO-only/No Treatment Control (NTC ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted total RNA was used to produce cDNA using the GoScript reverse transcriptase system (Promega) and qRT-PCR was subsequently performed using IQ SYBR green mix (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: Dry IgG was redissolved in 25 µL 25 mM ammonium bicarbonate and digested with 0.2 µg sequencing grade modified trypsin (Promega Corp., Madison WI, USA) at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed twice with 1X phosphate-buffered saline (PBS) and lysed using 1X passive lysis buffer (Promega). The luciferase activity was measured using the dual luciferase assay kit (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... puromycin selection and viability assay (CellTiter-Glo 2.0, Promega). Two pools of 1.2 · 108 cells each were transduced ...
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bioRxiv - Microbiology 2023Quote: ... Shigella conjugants that grew at 30°C and were Cb60 resistant were screened by PCR for the presence of lpxE and the large virulence plasmid using GoTaq (Promega) and primer sets lpxE-F/lpxE-R (lpxE ...
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bioRxiv - Microbiology 2023Quote: ... Go-Taq polymerase (Promega) was used for genetic screening ...
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bioRxiv - Microbiology 2023Quote: ... using GoTaq® qPCR Master Mix (Promega). Analyses were performed following the manufactureŕs protocol using gene-specific qPCR primers ...
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bioRxiv - Microbiology 2023Quote: ... The Dual luciferase miRNA target vector (pmirGLO) was purchased from Promega, Madison ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was measured with an CellTiter-Glo® Luminescent Cell Viability Assay (Promega) and values were compared to mock treatment ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... All amplicons were pooled and size-selected using ProNex Size-Selective Purification System (Promega). Cleaned pools were quantified on a Qubit Fluorometer and then ran on an Agilent Bioanalyzer to check sizing and purity ...
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bioRxiv - Microbiology 2023Quote: ... 100 μL of this suspension containing 106 parasites were transferred into black clear-bottom 96-well plates and serial 2-fold dilutions were performed in triplicate adjusting the final volume to 200 μL with 300 μg/mL of beetle luciferin (Promega, France). Luciferase activity was quantified after 10 min of incubation with an IVIS Spectrum imager (PerkinElmer) ...
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bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Molecular Biology 2023Quote: ... or nanoLuciferase (nLuc) levels that were generated by co-transfected pGL4.53 or pNL1.1 (Promega, #E5011, #N1441) plasmids ...
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bioRxiv - Molecular Biology 2023Quote: Luciferase assays were carried out using Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega #1620) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... RNA was reverse-transcribed into cDNA using Maloney murine leukemia virus reverse transcriptase Taq polymerase (Promega, Madison, WI, USA). For quantitative RT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... the cells were lysed using passive lysis buffer (Promega). The transduction efficiency was quantified by measuring the GFP fluorescence in the prepared cell lysates using a Tecan infinite 200 pro plate reader ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... luciferase activity was quantified using the bright glo luciferase assay system (Promega, Cat. #E2610).
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions (Promega). The PCR conditions were 25 °C for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... using GoTaq® qPCR Master Mix (Promega Cat. No. A6001) following ‘manufacturer’s instructions on a Veriti Thermo Cycler (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... chemiluminescent substrate or alkaline phosphatase (AP)-conjugated secondary antibody and Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega). Membranes were imaged using a ChemiDoc MP imaging system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Antisense probe was generated by cleavage of pSC-dmist plasmid with XbaI and in vitro transcribed with T3 polymerase (Promega P2083) using 1 µg DNA template according to the standard in vitro transcription protocol (see the full protocol at dx.doi.org/10.17504/protocols.io.ba4pigvn) ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Plant Biology 2023Quote: ... under the GloMax Discover plate reader system from Promega. Primers used in the dual-LUC assays were given in Supplementary Table 8.
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bioRxiv - Physiology 2023Quote: ... with adipose transcripts amplified with GoTaq® qPCR master mix (Promega, Cat. No A6001) using a ViiA 7 PCR system (Applied Biosystems ...