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Citations for Promega :
6901 - 6950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Molecular Biology 2023Quote: The psiCHECK-2 plasmid (Promega) has a special NheI restriction site that was used to insert a synthetic DNA duplex encoding the G-rich region of the PRCC-TFE3 fusion gene in the upstream of the renilla luciferase initiation codon to create the plasmids G4Q27 and G4M27 (mutated G-rich region of the PRCC-TFE3 fusion gene) ...
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bioRxiv - Molecular Biology 2023Quote: ... was digested with Not I restriction digestion enzyme (Promega) and transcribed using mMessage mMachine SP6 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were sampled by washing twice in PBS and lysed in Glo Lysis Buffer (Promega, E2661) for 5 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
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bioRxiv - Molecular Biology 2023Quote: ... the strips were washed in TBS-T for 15 min and incubated with the secondary antibody anti-mouse IgG-HRP (Promega, Cat. no: W4028) at 1:10000 dilution in TBS-T 3 % BSA for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ng/µL trypsin (Promega, USA) in water to saturate the inner surface and to prevent sample loss by nonspecific adsorption ...
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bioRxiv - Molecular Biology 2023Quote: ... Reduction, alkylation and in-gel digestion were performed as described (Shevchenko et al., 2006) except that Trypsin/LysC mix (Promega) was used instead of trypsin ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with CellTiter 96® AQueous One Solution Cell Proliferation Assay reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed and luciferase emission was measured using Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1:100 RNasin (Promega). Cells were then indirectly fluorescently immunolabeled against Somatostatin and Insulin and sorted ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HMT activity was evaluated using an MTase-Glo Methyltransferases Assay Kit (Promega). The luminescent signal that corresponds to the production of SAH was measured using synergy H1 microplate reader in a white 96-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... transfected cells grown for 48 hours in 96-well plates were washed with Hanks′ Balanced Salt solution (HBSS) and incubated with 1/1,000 dilution of furimazine stock solution (Promega, WI, USA). After incubation for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Bright-Glo reagent (Fluc Luciferase Assay System, Promega) was added to a final concentration of 5 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The vector was subsequently linearized by the DraI restriction digestion enzyme (Promega) and in vitro transcribed using mMessage mMachine T7 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
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bioRxiv - Molecular Biology 2023Quote: ... Based on the migration of the amplified bands in relation to the industry-standard 100-bp DNA ladder (Promega), the size of the bands was further estimated (in nucleotide base pairs).
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... treated with RQ1 Rnase-free Dnase (Promega), and converted to cDNA using the Promega Reverse Transcription system (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA were amplified with the GoTaq qPCR Master Mix (Promega) and the fluorescence was measure on a QuantStudio 6 Flex System (Applied Biosystems) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... and converted to cDNA using the Promega Reverse Transcription system (Promega). SYBR green RT-qPCR was performed in triplicate using cDNA and run on the Bio-Rad CFX384 real time thermocycler ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability and caspase 3/7 activity was measured with CellTiter Flo (Promega) or CaspaseGlo (Promega ...
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bioRxiv - Molecular Biology 2023Quote: Cell proliferation was measured with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of DNAsed RNA were retro-transcribed using random hexamer primers with the M-MLV-RT enzyme (Promega). cDNA were amplified with the GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... coated dishes with 30 ng/ml of mouse NGF (G5141, Promega). Then the neurons were microinjected with METTL3/14 activators (200 nl ...
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bioRxiv - Neuroscience 2023Quote: ... and converted to cDNA using the Promega Reverse Transcription system (Promega). SYBR green RT-qPCR was performed in triplicate using cDNA and run on the Bio-Rad CFX384 real time thermocycler ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: The P450-Glo™ CYP2D6 Assay and P450-Glo™ CYP3A4 Assay Luciferin-IPA (Promega) kits were used to measure Cytochrome P450 2D6 and P450 3A4 activity employing a luminometer (Lumat LB 9507 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2µg of total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... abd a GloMax plate-reader (Promega). Samples were prepared by boillling with 2x Laemmli sample buffer (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: Luciferase activity was analyzed 48 h post-transfection using DualGlo (Promega), essentially as previously described (25) ...
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bioRxiv - Neuroscience 2023Quote: After coelenterazine H (Coel H, Promega, 2.5 µM) addition ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Molecular Biology 2023Quote: ... primer extension reactions were done using AMV reverse transcriptase (Promega) and 32P-labeled primer (Pisarev et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Firefly luciferase activity was quantified using the Dual-Luciferase Reporter Assay System (Promega) and normalized against Gaussia luciferase activity.
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bioRxiv - Molecular Biology 2023Quote: ... The digoxigenin-labeled antisense and sense sybu riboprobes were synthesized from a SacII-or SacI-linearized pGEM-T easy (Promega), which contained an 823 bp cDNA-fragment from sybu-mRNA (NCBI Reference Sequence ...
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bioRxiv - Molecular Biology 2023Quote: Native total calf liver tRNA (Promega) and in vitro transcribed tRNAiMet were aminoacylated using recombinant E ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Microbiology 2023Quote: ... and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Microbiology 2023Quote: ... DNA extraction of fecal cultures was performed using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega AG, Dübendorf, Switzerland). Library preparation ...
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bioRxiv - Microbiology 2023Quote: ... and analysed using the Dual-luciferase® Reporter Assay System (Promega), a FLUOstar Omega Microplate reader and Optima software (BMG LABTECH).
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bioRxiv - Microbiology 2023Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega).
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed at the indicated timepoints using passive lysis buffer (Promega) and analysed using the Dual-luciferase® Reporter Assay System (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...