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Citations for Promega :
6801 - 6850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cell death was measured by the LDH assay using CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) as per manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Assays for measuring cell toxicity were purchased from Promega (Madison, WI, USA). Anti-FLAG primary antibody was purchased from Sigma-Aldrich (#F1804) ...
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bioRxiv - Biochemistry 2023Quote: ... penicillin 100 U/ml and streptomycin 100 μg/ml) +/-HaloTag® NanoBRET™ 618 Ligand (0.5 μl/ml, Promega). 4 h later ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8M urea in PBS and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071) with vortexing ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Bioengineering 2023Quote: ... Fluc luminescence was measured 72 h after co-culture using the Bright-Glo Luciferase Assay System (Promega, E2610) and a Tecan Infinite M200 Pro plate reader ...
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bioRxiv - Bioengineering 2023Quote: The luminescence of each well was measured 24 h later using the Bright-Glo substrate (Promega, E2610) and a Tecan Infinite M200 Pro plate reader.
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bioRxiv - Biochemistry 2023Quote: ... Enzymatic digestion and detergent removal were performed using the S-Trap device and Trypsin Gold (Promega, Madison, Wisconsin, USA) following the manufactures recommended protocol ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega), and mixed gently at 37°C overnight to digest proteins ...
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bioRxiv - Biochemistry 2023Quote: ... The firefly luciferase activity was measured using luciferase assay reagent (Promega) according to manufacturer’s protocol on a Synergy Mx microplate reader (BioTek).
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing grade modified trypsin was purchased from Promega (Madison, Wisconsin, USA). MagReSyn® HILIC microspheres were purchased from ReSyn Biosciences (Edenvale ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Bioengineering 2023Quote: ... One microgram of RNA was converted to cDNA using ImProm-II™ Reverse Transcription System (PROMEGA, Madison, WI, USA) following the manufacturer’s instructions performed on T100™ Thermal Cycler (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was visualized using 500 μL of 1:1000 NanoGlo substrate (Promega, catalogue number: N1110).
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Biochemistry 2023Quote: ... 200 nM of biotinylated Arr3_ýC·IP6 complex was immobilized on 250 µl Streptavidin magnetic beads (Promega, Cat No: Z5482) and incubated with 100 μl of a phage library E (Miller et al. ...
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bioRxiv - Bioengineering 2023Quote: ... using a microplate reader (Glomax, Promega, Southampton, UK). Based on a standard curve ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Luciferase and renilla activities were measured with a dual-LUC Reporter Assay System (Promega, Madison, WI, USA) after 2 days of incubation in the dark and 1 day of cultivation under normal growth conditions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase inhibitors (Promega N2615 and Life Technologies AM2696)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 25 mM MgCl2 (Promega), 10 µM P7/P8 and either 50 µM oxo- dGTP or 1 µM dPTP (Jena Bioscience ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted using Wizard® Genomic DNA Purification Kit (Promega, A1120). After measuring DNA quantity by Qubit (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... And was then treated with DNase I (Promega). First-strand cDNA was then synthesized using M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Genetics 2023Quote: ... 0.2% SDS (Promega) and 1 mg/ml Proteinase K (Merck ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: ... We used lipofectamine 2000 to transfect 106 NIH-3T3 MEFs with 3 μg of the luciferase plasmid with a WT DBS or its mutant counterpart and 30 ng of a renilla luciferase expression plasmid (pGL4.73, Promega) for normalization ...
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bioRxiv - Genetics 2023Quote: ... cells were harvested and assayed using Dual-Glo® luciferase assay system (Promega). The luciferase activities were measured using luminescence reader (Mplex (Infinite M200) ...
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bioRxiv - Genetics 2023Quote: CellTiter 96 AQueous One Solution (Promega G3580) was added to fresh BM-MSC complete growth media ...
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Genetics 2023Quote: The input material for Cas9-directed LRS was the remaining Bionano-isolated ultra-high molecular weight DNA or Maxwell RSC (AS1400) (Promega, Madison, WI) or Nanobind CBB (Pacific Biosciences ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was isolated using the Promega Wizard gDNA kit (A1120; Promega) as directed ...
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bioRxiv - Genomics 2023Quote: ... minCMV (27) and minP (derived from pGL4 (Promega, Madison, WI, USA)) ...
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bioRxiv - Genomics 2023Quote: ... The guide and donor plasmids were transfected into cells using Fugene HD (Promega). After seven days ...
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bioRxiv - Genomics 2023Quote: ... PCR products were purified via gel excision and column purification (Promega, A9282), followed by insertion into the BsmBI–digested lentiV2-hU6-evopreQ1 vector by Gibson assembly (NEB ...
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bioRxiv - Genomics 2023Quote: ... All PCR analyses were performed on 0.5 ng cDNA using 0.5 U GoTaq® DNA polymerase (Promega). Quantification of PCR products was performed using the Image Lab software (BioRad ...
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bioRxiv - Genomics 2023Quote: ... we replaced the Luc2-IRES-ZsGreen reporter with a cassette encoding H2B fused to Nanoluciferase (Promega) to minimize background luminescence ...
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bioRxiv - Genomics 2023Quote: ... before 100 µL nanoluciferase substrate (Nano-Glo, Promega, N1110) were added to each well ...
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bioRxiv - Neuroscience 2023Quote: ... and RNase inhibitor (Promega N2515). We designed male-specific and female-specific forward RT-PCR primers ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: Viral capped mRNA (rat HEV) was made from linearized DNA using the Promega Ribomax Large Scale RNA Production System SP6 (Promega PRP 1300) and ARCA CAP (TriLink Biotechnologies) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of modified trypsin (Promega) was added ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...