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Citations for Promega :
6601 - 6650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Immunology 2023Quote: ... and luciferase activity was measured using Luciferase Assay System (Promega).
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bioRxiv - Microbiology 2023Quote: ... DNA samples were treated with a final concentration of 0.2mg/ml RNaseA (Promega #A7973) and 0.2mg/ml Proteinase K (NEB #P8107S ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Bioengineering 2023Quote: ... the GoTaq qPCR Master Mix (Promega) was used with input template 4ng cDNA per reaction while the reaction was detected on a CFX 96 Real-Time PCR detection system (BIO-RAD) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 0.2 mM of each dNTP and 0.02 U/μL G2 Taq polymerase (Promega). All cycles consisted of 5mn at 94°C followed by 20 (first PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega, USA). In parallel experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Dual-Glo® Luciferase Assay System (Promega, USA) was used to detect the luciferase activities ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Plasmids were transfected into HEK293T cells using the ViaFect™ Transfection Reagent (Promega) according to manufacturer instructions 45.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed with M-MLV reverse transcriptase using random primers according to the manufacturer’s protocol (Promega, Madison, USA). qRT-PCR was performed in a 25 μl reaction volume containing ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was treated with RQ1 RNase-free DNase (Promega) prior to cDNA synthesis ...
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bioRxiv - Plant Biology 2023Quote: Luciferase levels were detected using the Dual-Luciferase® Reporter Assay System (Promega, Madison, USA). Cells were centrifuged at 850 xg for 10 min and the supernatant was discarded ...
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bioRxiv - Plant Biology 2023Quote: ... The LUC activity was assayed in the supernatant using the Luciferase Assay System (Promega, USA). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Injectors were loaded with either NanoGlo reagent (Promega, 1:250 dilution in imaging buffer) or test compounds and addition was automated by the plate reader ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... neutralized with 1M Tris solution and lysates were further processed for measurement using the Glucose-Glo Assay (Promega, GmbH) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... the cells were lysed using passive lysis buffer (Promega). The transduction efficiency was quantified by measuring the GFP fluorescence in the prepared cell lysates using a Tecan infinite 200 pro plate reader ...
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bioRxiv - Immunology 2023Quote: ... and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using FuGENE HD (Promega, Madison, WI). Twenty-four htr after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1 μL/mL CellTox Green (Promega). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: Glucose uptake was detected by 2-DG uptake assays using a Glucose Uptake-Glo™ Assay (Promega, J1341, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 nM JF646 ligand (Promega) was added 15 min prior to imaging ...
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bioRxiv - Cancer Biology 2023Quote: Glutathione estimation was performed according to the manufacturer’s instructions using GSH-Glo™ Glutathione Assay kit (Promega, USA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... 1μl of ATP (100mM, Promega®), 0.5μl of GTP (100mM ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Biochemistry 2023Quote: ... 5 ng pRL-SV40 (Promega), and 375 ng total amount of testing plasmids individually or in combinations ...
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bioRxiv - Biochemistry 2023Quote: ... in a Glomax 20/20 luminometer (Promega), as per manufacturer’s directions ...
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bioRxiv - Biochemistry 2023Quote: ... Mass spectrometry grade trypsin was obtained from Promega (Promega Corporation, Madison, WI, USA). Reagents used for enzymatic digestion (1,4-Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2023Quote: ... Firefly-Luciferase and Renilla-luciferase activities were determined using the Dual-Luciferase Reporter Assay System (Promega) in a Glomax 20/20 luminometer (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... Then after another 24 hr cells were lysed and luciferase activity was measured by using the luciferase assay system (Cat no E4030) from Promega.
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bioRxiv - Biochemistry 2023Quote: ... site directed mutagenesis was performed using Pfu DNA polymerase as per manufacturer’s instructions (Promega, Cat. # M7741). The resulting sequence verified plasmids having the desired mutation were individually transformed into E ...
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bioRxiv - Biochemistry 2023Quote: ... Nitrite concentration in spent media was measured using Griess Reagent System (Promega, G2930) per manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence from HiBiT-tagged proteins was measured with GloMax Navigator (Promega). Luminescence from CMs and lysates of nontransfected cells were used for background correction.
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bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
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bioRxiv - Biochemistry 2023Quote: Split-luciferase assay was performed using the Nano-Glo Luciferase Assay System (Promega). The coding sequence of small-BiT was fused to the gene of peptide binders and the coding sequence of large-BiT was fused to the coding sequence of the target peptide (Genscript) ...
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Biochemistry 2023Quote: ... The methyltransferase reaction product S-adenosylhomocysteine was quantified using MTase-Glo kit (Promega, Wisconsin, USA) following the manufacturer’s protocol (43) ...
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bioRxiv - Biochemistry 2023Quote: ... U2OS PINK1 KO cells seeded at a density of 40,000 cells per 18mm glass coverslip in a 12 well plate were transiently transfected with 0.5 ug of plasmid DNA expressing WT-HA PINK1 under the control of a CMV weakened promoter using FuGene HD (Promega, E2311) at a ratio of 1:3 ...
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bioRxiv - Biochemistry 2023Quote: ... TSAP and sequencing-grade modified trypsin were purchased from Promega. Recombinant kinases were purchased from Carna Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... cells were replaced with 90 μL fresh growth medium supplemented with 10 μL MTT reagents (Promega), followed by incubation at 37 °C for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega). All experiments were performed in biological triplicates.
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Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... cell proliferation was measured by CellTiter-Glo (CTG) Luminescent Cell Viability Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... viability was measured by Cell Titer Glo 2.0 assay (Promega) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP Conjugate (cat no. W4021) were purchased from Promega. Goat anti-Rat IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter 96(R) AQueous MTS Reagent Powder were provided by Promega (Madison, Wisconsin, USA). NdCl3 (>99% ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase digested and reverse-transcribed using the Improm-II Reverse Transcription System (Promega). qPCR was performed from 100 ng of gDNA using the Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: In ovo tumors were collected and lysed in the buffers for the GSH/GSSG-Glo assay kit (Promega) according to the manufacturer’s instructions and normalized for protein concentration (Pierce BCA protein assay kit ...