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Citations for Promega :
6551 - 6600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... obtained using GLoMax plate reader (Promega), was interpolated against the standard curve to calculate the neurotrophin concentration ...
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bioRxiv - Neuroscience 2023Quote: ... GloSensor-22F plasmid was obtained from Promega Corporation (E2301) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HMT activity was evaluated using an MTase-Glo Methyltransferases Assay Kit (Promega). The luminescent signal that corresponds to the production of SAH was measured using synergy H1 microplate reader in a white 96-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... transfected cells grown for 48 hours in 96-well plates were washed with Hanks′ Balanced Salt solution (HBSS) and incubated with 1/1,000 dilution of furimazine stock solution (Promega, WI, USA). After incubation for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Bright-Glo reagent (Fluc Luciferase Assay System, Promega) was added to a final concentration of 5 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The vector was subsequently linearized by the DraI restriction digestion enzyme (Promega) and in vitro transcribed using mMessage mMachine T7 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
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bioRxiv - Molecular Biology 2023Quote: ... Based on the migration of the amplified bands in relation to the industry-standard 100-bp DNA ladder (Promega), the size of the bands was further estimated (in nucleotide base pairs).
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bioRxiv - Molecular Biology 2023Quote: ... HRP conjugate (Promega, W4021) used at dilution 1:5000 for Western Blot
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bioRxiv - Molecular Biology 2023Quote: Plasmids used as DNA template in PCR reactions to generate donor DNAs for gene deletion/disruption strategies were obtained by amplifying blasticidin S deaminase (BSD) and puromycin-N-acetyltransferase (PAC) genes and cloning them individually into pGEM®-T Easy Vector (Promega). The reverse primers to amplify both genes included GTGA as four last nucleotides ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions (Promega). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Trypsin/LysC (2.4 μg) (Promega) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Transient transfections were performed using Viafect transfection reagent (E4981-Promega, USA) following the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2023Quote: ... and random primers (Promega). RT-qPCR was performed using Power SYBR Green (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... Dual Luciferase assay kit and PsiCHECKTM-2 vector were purchased from Promega. Oligonucleotides were synthesized and obtained from Eurofins Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted for HaloTag7 (Promega G928A) and Rpt3 (Abcam 140515-1001) ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μg of sequencing-grade trypsin (Promega) was added and the mixture was incubated for 1 hour at 47 °C ...
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bioRxiv - Molecular Biology 2023Quote: The inserts were amplified from the HaloTag-PSMD3 fusion vector (Promega N2701). 10 ng of template DNA was combined with PrimeStarMax DNA Polymerase (Takara #R045Q ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Molecular Biology 2023Quote: The psiCHECK-2 plasmid (Promega) has a special NheI restriction site that was used to insert a synthetic DNA duplex encoding the G-rich region of the PRCC-TFE3 fusion gene in the upstream of the renilla luciferase initiation codon to create the plasmids G4Q27 and G4M27 (mutated G-rich region of the PRCC-TFE3 fusion gene) ...
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bioRxiv - Cell Biology 2023Quote: Trypsin lysis was carried out by first rehydrating the bands in 300ng trypsin (Sequencing Grade Modified Trypsin (Promega Product no. V5111) in 60μl 100mM NH4HCO3 and incubated overnight at 37°C ...
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bioRxiv - Genomics 2023Quote: ... one replicate was used to determine cell viability using CellTiter-Glo® (Promega) according to the manuals ...
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bioRxiv - Molecular Biology 2023Quote: ... was digested with Not I restriction digestion enzyme (Promega) and transcribed using mMessage mMachine SP6 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were sampled by washing twice in PBS and lysed in Glo Lysis Buffer (Promega, E2661) for 5 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1:100 RNasin (Promega). Cells were then indirectly fluorescently immunolabeled against Somatostatin and Insulin and sorted ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Gel pieces were reduced (DTT) and alkylated (iodoacetamide) and subjected to enzymatic digestion with sequencing grade trypsin (Promega) overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and stained in Diamond Nucleic Acid Stain (Promega, H1181). All experiments were performed in 8-12 week old animals ...
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bioRxiv - Neuroscience 2023Quote: ... Both HEK293T cells and N2A cells were transfected with indicated plasmids via Fugene (Promega, E2311) as previously described46 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5U/uL RNasin (Promega, N2615), 0.5U/uL SUPERase RNase inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was reverse transcribed using random decamers and M-MLV reverse transcriptase (Promega)/Superscript II RNase H reverse transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged and resuspended in EZ lysis buffer, followed by three washes in wash/resuspension buffer (1x PBS, 1% BSA, 0.2U/μL RNase Inhibitor (Promega)) and labeled with propidium iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was isolated with the Maxwell 16 DNA Tissue DNA Purification Kit (AS1030, Promega) following the manufacturer’s protocal ...
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bioRxiv - Neuroscience 2023Quote: ... Frozen tissue was placed into a petri dish on ice and cut into 0.2cm cubed pieces or smaller using clean scissors and forceps in 1mL of HB (0.25M sucrose, 25mM KCl, 5mM MgCl2, 20mM Tricine-KOH pH 7.8, 0.1U/uL RNasin (Promega, N2615), 0.5U/uL SUPERase RNase inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... mouseStoml3-GFP10::mCHERRY and mouseElkin1-GFP11::eBP2 plasmids using Fugene HD and following manufacturer’s instructions (Promega). 7 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... after which the slice pieces were fixed in 4% PFA in PBS (with 0.1 U/ml RNase inhibitor, Promega) for 15 mins and dissociated into single cells through careful trituration ...
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bioRxiv - Neuroscience 2023Quote: ... and Oligo(dT)15 primers (Promega) according to manufacturer’s instructions in a ProFlex PCR machine (Applied Biosystems).
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bioRxiv - Neuroscience 2023Quote: ... plus helper plasmids pVSV-G (18 mg) and psPAX2 (27 mg) using FuGENE HD (Promega). DNA was incubated with 210 ml FuGENE HD in 4.5 ml Opti-MEM (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... ROS-Glo H2O2 Assay (Promega, G8820). All other chemicals were obtained from Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 µg/ml herring sperm DNA (Promega), 50 µg/ml heparin ...