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Citations for Promega :
6701 - 6750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: qPCR was performed using the intercalating dye based GoTaq qPCR master mix kit (Promega). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...
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bioRxiv - Neuroscience 2023Quote: ... and then retrotranscribed into cDNA using M-MLV reverse transcriptase (Promega) and Oligo(dT)15 primers (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested vector was subjected to invitro transcription (IVT) following the manufacturer’s protocol using T7 polymerase (Promega P2075) and biotin RNA labeling mix (Roche 11685597910).
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bioRxiv - Microbiology 2023Quote: ... 2 μL of furimazine substrate was added to the reaction well from a working reagent stock of a 2:100 NanoDLR Stop & Glo Substrate to Buffer ratio (Promega #N1610). A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin digest was allowed overnight at 37°C by the addition of 1 μg of trypsin (#V5111, Promega) to each sample ...
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bioRxiv - Microbiology 2023Quote: ... and then 50 μl of 5% (v/v) SteadyGlo reagent (Promega) in glycine buffer (50 mM glycine ...
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bioRxiv - Microbiology 2023Quote: ... Native firefly luciferase (Promega) was diluted in holdase buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... and cloned into the pCI plasmid (Promega, Madison, WI). Large-scale DNA plasmid was amplified in Escherichia coli DH5α and purified by using a plasmid giga prep kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance values were acquired using a GloMAX® Explorer microplate reader (Promega) at 600 nm ...
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bioRxiv - Genomics 2023Quote: ... in the presence of 0.02% ProteaseMax (Promega) overnight ...
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bioRxiv - Genomics 2023Quote: ... We examined the bisulfite conversion rate using unmethylated lambda phage DNA (Promega, D1521).
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega, Madison, WI, USA). RT-qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd. ...
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bioRxiv - Microbiology 2023Quote: Translation reactions were performed using the TNT Quick Coupled Transcription/Translation kit (Promega). 10-50 ml reactions were assembled according to kit directions and labelled using EasyTag L-[35S]-Methionine (Perkin-Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNAs were isolated using the ReliaPrep™ gDNA Tissue Miniprep System (Promega) following the Mouse tail protocol ...
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bioRxiv - Microbiology 2023Quote: ... we added 50 µl of Bright-Glo Luciferase Assay lysis/substrate buffer (Promega) and measured luminescence within 10 min using a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Microbiology 2023Quote: ... in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega). After overnight incubation at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... the CellTox Green Cytotoxicity Assay Kit from Promega was performed using a modified version Endpoint Step Protocol ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: Viral capped mRNA (rat HEV) was made from linearized DNA using the Promega Ribomax Large Scale RNA Production System SP6 (Promega PRP 1300) and ARCA CAP (TriLink Biotechnologies) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of modified trypsin (Promega) was added ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... measured using a Biotek Synergy 2 multimode plate reader and the BacTitr-Glo Cell Viability assay (Promega). There was a linear relationship between concentration and luminescence (R2 =0.998) ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 0.1M TEAB and overnight (∼16 h) digestion with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C was performed with constant mixing using a thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... and a GloMax+ plate reader (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Microbiology 2023Quote: ... cell viability was assayed using CellTiter-Glo (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Luminescence measurements were acquired using Nano-Glo Luciferase Assay System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were harvested into 30 µl passive lysis buffer (PLB; Promega), incubated for 15 min at room temperature and stored at -80°C until assayed ...
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bioRxiv - Neuroscience 2023Quote: Glucose levels in culture media were quantified using a bioluminescent NADH detection assay according to manufacturer’s instructions (Glucose-glo, Promega). Briefly ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the TMB substrate (Promega) was added and incubated before the reaction was stopped using 1 M sulfuric acid ...
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bioRxiv - Immunology 2023Quote: ... into the culture medium was quantitated using Cyto Tox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA) as described previously (20) ...
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bioRxiv - Immunology 2023Quote: The release of lactate dehydrogenase (LDH, Promega, USA) into the culture medium was quantitated using Cyto Tox 96® Non-Radioactive Cytotoxicity Assay (Promega ...
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bioRxiv - Microbiology 2023Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) was extracted using the Wizard gDNA purification kit (Promega Corporation). From the 35 wild-type strains that were cured from pOXA-48 ...
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bioRxiv - Molecular Biology 2023Quote: ... and western blue stabilized substrate for alkaline phosphatase (Promega, Madison, WI, USA) was used for chromogenic visualization of protein bands.
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding the Firefly luciferase sequence in negative polarity flanked by the 5’ and 3’ non-coding regions of the IAV NS segment was used and the pTK-Renilla plasmid (Promega) was used as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding a negative-sense viral-like RNA expressing the Firefly luciferase and the pTK-Renilla plasmid (Promega) as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed 20-24 hpt in Renilla lysis buffer (Promega) for 45 min at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... princeps luciferase enzymatic activity due to luciferase reconstitution was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies) using a reading time of 10 s after injection of 50 µl Renilla luciferase reagent (Promega). Mean relative light units (RLUs ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Protein digestion was achieved with 2.5 µg of trypsin (Promega) and overnight incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves from the amplification of the cloned target sequence in a pGEM-T vector (Promega) were used to quantify 16S total copy number per sample.
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bioRxiv - Microbiology 2023Quote: Cell pellets resulting from later experiments (optimization of medium composition and fiber- and drug-testing) were pre-processed using the Maxwell® HT 96 gDNA Blood Isolation Kit (Promega AG, Dübendorf, Switzerland) combined with a FastPrep homogenizer (FastPrep-24TM ...